池沼公鱼耳石日轮的观察研究

于1989年3－8月，从辽阳汤河水库捕取成熟的池沼公鱼，获得人工授精卵。用压片法光镜下连续观察胚胎发育后期和前仔鱼期耳石轮纹的形成，并采集已知月龄的幼、成鱼耳石，制片镜检轮纹数量和排列。结果表明，仔鱼在印黄接近吸收完并开始摄食外界食物时，即孵出后第6d至第7d形成第1个轮纹，之后每天一轮，这些轮纹就是日轮；日轮间距呈规律性变化，这些变化与鱼体生长发育及生态条件密切相关。     

Factors involved in growth plasticity of cockles Cerastoderma edule (L.), identified by field survey and transplant experiments

In Arcachon Bay, a macrotidal coastal lagoon in southwest France, growth rates of cockles Cerastoderma edule (L.) differ greatly between locations. Sampling of populations at different tidal levels showed that the mean shell length was significantly and positively correlated with immersion time, whereas no correlation was found with population density and microphytobenthos biomass. Transplants of cockles between two intertidal sites were used to examine the relative importance of habitat and site of origin for growth rate and condition index. Artifacts due to manipulation were assessed, i.e. the impact of enclosures on growth, conditon index and mortality. During a 5-mo reciprocal transplant experiment, growth rates of the transplanted cockles and the cockles already present were similar, whereas the sites of origin did not affect growth rate. Condition index, however, displayed significant differences in relation to both transplant and origin sites. During a further 6-mo experiment in which cockles were transplanted from one site to four new sites, growth was mainly influenced by tidal level. These results indicate that net growth started when cockles were immersed 30% of the time. It is concluded that, on the scale of Arcachon Bay (156 km²), differences in growth and condition between cockle populations are largely phenotypic. Tidal level (i.e. food supply and emersion stress) accounts for most of these differences, but the existence of genetically or physiologically different populations cannot be excluded.     

Upstream dams and downstream clams: growth rates of bivalve mollusks unveil impact of river management on estuarine ecosystems (Colorado River Delta, Mexico)

We studied how the extensive diversion of Colorado River water, induced by dams and agricultural activities of the last 70 years, affected the growth rates of two abundant bivalve mollusk species (Chione cortezi and Chione fluctifraga) in the northern Gulf of California. Shells alive on the delta today (‘Post-dam’ shells) grow 5.8–27.9% faster than shells alive prior to the construction of dams (‘Pre-dam’ shells). This increase in annual shell production is linked to the currently sharply reduced freshwater influx to the Colorado River estuary. Before the upstream river management, lower salinity retarded growth rates in these bivalves. Intra-annual growth rates were 50% lower during spring and early summer, when river flow was at its maximum. Growth rates in Chione today are largely controlled by temperature and nutrients; prior to the construction of dams and the diversion of the Colorado River flow, seasonal changes in salinity played an important role in regulating calcification rates.Our study employs sclerochronological (growth increment analysis) and geochemical techniques to assess the impact of reduced freshwater influx on bivalve growth rates in the Colorado River estuary. A combination of both techniques provides an excellent tool to evaluate the impact of river management in areas where no pre-impact studies were made.     

Ecological characteristics of Walleye pollock eggs and larvae in the southeastern Bering Sea during the late 1970s

Walleye pollock (Theragra chalcogramma) is an ecologically and economically important groundfish in the eastern Bering Sea. Its population size fluctuates widely, driving and being driven by changes in other components of the ecosystem. It is becoming apparent that dramatic shifts in climate occur on a decadal scale, and these “regime shifts” strongly affect the biota. This paper examines quantitative collections of planktonic eggs and larvae of pollock from the southeastern Bering Sea during 1976–1979. Mortality, advection, and growth rates were estimated, and compared among the years encompassing the 1970s’ regime shift. These data indicate that pollock spawning starts in late February over the basin north of Bogoslof Island. Over the shelf, most spawning occurs north of Unimak Island near the 100 m isobath in early or mid April. Pollock eggs are advected to the northwest from the main spawning area at 5–10 cm/sec. Larvae are found over the basin north of Bogoslof Island in April, and over the shelf between Unimak Island and the Priblof Islands in May. Compared to 1977, the spawning period appeared to be later in 1976 (a cold year) and earlier in 1978 (a warm year) in the study area. At the lower temperatures in 1976, egg duration would be longer and thus egg mortality would operate over a longer period than in the other years. Mean larval growth appeared to be lower in 1976 than in 1977 and 1979. Estimated egg mortality rate in 1977 was 0.6 in April and 0.3 in early May.     

Particulate peptidoglycan in seawater determined by the silkworm larvae plasma (SLP) assay

Peptidoglycan (PG) is a biopolymer found exclusively in the cell wall of bacteria. Recent chemical analysis of particulate organic matter suggests that a major amount of the muramic acid, an amino sugar present only in PG, could not be accounted for in terms of bacterial cells (Benner and Kaiser, 2003); however, data on particulate PG is quite sparse. In the present study, conducted in 1996, the PG was examined at 5 sampling sites in the northwestern Pacific Ocean, and in natural seawater cultures. Particulate PG, which was concentrated using a 96-well filtration plate equipped with Durapore filters (pore size, 0.22 μm), was measured by the silkworm larvae plasma (SLP) assay. The PG concentration generally decreased with depth and correlated significantly with bacterial abundance throughout the entire water column. However, the ratio of particulate PG to bacterial abundance varied with depth. The average ratio was 0.61 ± 0.53 (average ± SD, n = 40) between 50 and 2000 m, which agreed with the bacterial cellular PG content from 0.63 to 1.1 fg cell⁻¹ obtained in seawater cultures. On the other hand, the ratios of PG to bacteria from the surface to 50 m (3.7 ± 2.6, n = 29) and below 2,000 m (2.1 ± 1.7, n = 7) were significantly higher than that between 50 and 2,000 m. These results may suggest that, in the surface and deep layers, a significant fraction of particulate PG was present in bacterial detritus, whereas this fraction was reduced in the middle layer.