手机信令数据支撑下广州市过剩通勤及其空间分异格局

利用手机信令数据构建居委会尺度下的职住联系数据库,分析广州过剩通勤及其空间分异格局。研究发现,广州市过剩通勤为76.01%,与国内和西方国家城市相比处于较高水平,说明广州市职住空间组织效率偏低。过剩通勤的空间分异特征主要表现为：功能相对单一的郊区大型工业或房地产集聚区过剩通勤率偏高;传统市中心虽然就业机会集中,但因为高居住成本也出现较高的过剩通勤率;近郊区的特定功能区域,如大学城、偏农业发展型城郊村等区域出现较低的过剩通勤率;快速轨道交通（地铁）建设因降低了居民对通勤距离的敏感度而导致过剩通勤水平的提升,高过剩通勤率空间分布与地铁沿线和地铁站点分布存在一定的空间一致性。提升大城市的职住空间组织效率除需重点考虑提升区域职住空间平衡外,优化城市功能结构、降低住房成本区域差异等也需予以重点考虑。     

Representing environments in flux: case studies from East Africa

The dominant view in the ecology and anthropology of the 1950s saw populations harmoniously interacting in self-regulating systems; climax forests and stable societies were the ruling hypotheses. Now, however, ecology and social sciences are investigating nature and culture in flux. The flux paradigms of nature and culture describe a human–ecological relationship that is non-equilibrial, historically contingent and constantly negotiated at both material and ideological levels by unequal actors. In this paper, we examine the effect of changing ecological and cultural paradigms on interpretations of environmental change in three areas of East Africa: the North Pare Mountains, Tanzania, the Mkomazi Game Reserve, Tanzania and the Tsavo National Park, Kenya. We explore how discursive and materialist approaches can complement one another, by expanding the domains of ecological inquiry and demanding that analysts cross-check their data for unquestioned assumptions regarding stability, variability and spatial and temporal scales. Rather than testing a ruling hypothesis, we suggest that ecologists and social scientists work with multiple hypotheses, with the aim of understanding the interplay between ecological, environmental and social influences.     

Strain and culture medium optimization for production enhancement of prodiginines from marine-derived Streptomyces sp. GQQ-10

A mutant (GQQ-M6) of a Sponge-Derived streptomyces sp. GQQ-10 obtained by UV-induced mutation was used for producing prodiginines (PGs). Single factor experiments and orthogonal array design (OAD) methods were employed for medium optimization. In the single factor method, the effects of soluble starch, glucose, soybean flour, yeast extract and sodium acetate on PGs production were investigated individually. In the subsequent OAD experiments, the concentrations of these 5 key nutritional components combined with salinity were further adjusted. The mutant strain GQQ-M6 gave a 2.2-fold higher PGs production than that of the parent strain; OAD experiments offered a PGs yield of 61mg L-1, which was 10 times higher than that of the initial GQQ-10 strain under the original cultivation mode.     

转兔防御素基因小球藻培养的营养模式研究

在250mL摇瓶中研究了光自养、异养、混合营养3种营养模式对转兔防御素基因(NP-1)小球藻(Chlorella)的生长、NP-1表达量、总蛋白质含量、pH、葡萄糖消耗以及光衰减等几个方面的影响。结果表明,异养培养为转NP-1基因小球藻的最适营养模式。在此基础上,采用5,15,30L生物反应器补料分批异养培养转NP-1基因小球藻,细胞质量浓度分别达到3.67,4.87,6.39g/L。     

CHIMEHERB中的有效成分对表皮细胞培养生长的影响

为观实CHIMEHERB有效成分,对新生小白鼠和人包皮外板的表皮细胞的生长影响,本文对7个实验浓度13次实验,112组592个组织块进行了组织培养的资料分析和机理的初步探讨,结果说明CHIMEHERB对表皮细胞无任何毒副作用,能100％的促进表皮细胞的生长.其相对生长率:小白鼠组为142.73％,人包皮外板组为440.58％,实验结果与临床应用效果一致.     

海鞘血细胞培养和细菌污染控制

以海鞘(Ciona savignyi)为研究对象,考察了海鞘血细胞原代培养方法及培养中细菌污染的鉴定和控制.血细胞在培养1 h后贴壁,细胞基本以圆形细胞为主,变形细胞一般呈不规则形.变形细胞存活时间较短,而圆形细胞存活时间较长.对于培养过程中的细菌污染,通过细菌分离、培养和纯培养发现两类菌株检出率较高,均为革兰氏阴性菌.经PCR扩增16S rDNA基因序列片断,结果显示这两类菌株分别属于弧菌属(Vibrio)和施万氏菌属(Shewanella).药敏试验结果显示,弧菌属对氯霉素和环丙沙星等具有较强的敏感性;对施万氏菌属较敏感的药物依次为亚胺培南和氯霉素等.最后比较了几种抗生素组合控制血细胞培养中细菌污染的使用效果,其中氯霉素、亚胺培南加双抗的组合有较好的抑菌效果并对培养细胞的贴壁和生长没有影响.     

胶州湾女姑山水域夏季赤潮高发期浮游植物群落结构特征

根据 1998年 5～ 8月夏季赤潮高发期间 ,胶州湾女姑山水域浮游植物群落结构的种群组成特征和演替变化对赤潮的发生和种群变动特点进行初步研究。结果表明 ,夏季女姑山水域的浮游植物主要由硅、甲藻组成 ,硅藻在细胞和物种丰度上都占优势。浮游植物优势种在调查期间出现明显的演替现象 ,水温的升高和营养条件的变化是引起物种演替的主要原因。浮游植物细胞丰度在夏季呈明显的增长趋势 ,从 5月份 6.3× 10 5个cell/m3 增至 8月份的 6.7× 10 6个cell/m3 ;其中 ,由于赤潮的爆发 ,细胞丰度的最高峰出现在 7月份 ,为 1.5× 10 7个 /m3 。在赤潮爆发期间 ,赤潮原因种高齿状藻和中肋骨条藻发生了明显的演替现象 ,不同细胞大小的优势种和营养条件的变化是发生物种演替的主要原因。叶绿素a的浓度和细胞丰度的变化共同反映了赤潮发生的全部过程。     

Effect of slow-solvent-vapour treatment on performance of polymer photovoltaic devices

In this work, enhanced poly(3-hexylthiophene):[6,6]-phenyl-C61-butyric acid methyl ester (P3HT:PCBM) bulk-heterojunction photovoltaic devices are achieved via slow-solvent-vapour treatment. The correlations between the morphology of the active layer and the photovoltaic performance of polymer-based solar cell are investigated. The active layers are characterized by atomic force microscopy and optical absorption. The results show that slow-solvent-vapour treatment can induce P3HT self-organization into an ordered structure, leading to the enhanced absorption and efficient charge transport.     

凡纳滨对虾性别分化的初步研究

通过组织学连续切片与扫描电镜观察,研究凡纳滨对虾的性别分化。结果显示:凡纳滨对虾外部形态的性别分化最早发生在仔虾后第37天,此时在扫描电镜下可观察到雄性附肢的芽基,仔虾后第45天,光镜下可观察到交接器;仔虾后第24天出现原始生殖细胞(PGCS),位于背大动脉的两侧及围心腔膜的腹面,细胞个体及细胞核明显比周围的细胞大,具有强嗜碱性,细胞核圆形或椭圆形,核内染色质清晰可见,随着生殖腺的发育,在第43天出现生殖管,第55天生殖腺可区分为精巢和卵巢。     

加强生态环境保护 促进湛江珍珠养殖业持续发展

为促进湛江珍珠养殖业的持续发展,各级政府和海域管理部门应认真贯彻执行国家有关海域管理的法律法规,加强海域环境保护的宣传教育工作,提高养殖者的环境保护意识。珍珠养殖者要创新养殖方式,拓展新的养殖空间,尊重自然规律,自觉保护海洋生态环境,以解决湛江珍珠养殖业存在的养殖海域无证使用、无度开发、生态功能丧失等问题。