Bioreactor technology for production of valuable algal products

INTRODUCTIONManystudiesonvascularplantSduringthenontwperiodhavedrireP0redinChinaandabroad.TherearefeWreportSonwhethertherearesomevasctilarplantS,andhowmanyspedessurvive,underice,andontheirbiomassesandphotosyntheticproduCtivitiesduringwinter,espedal1yduringbeAfpinchLillyamepe0ylenctal.,l976,Liuetal.,l994).WeimtlycarriedoutaseadriontheunderforvascularplantsdurinwinderinsomewaterbodieswherevascularplantSgrowluxdrianhydurinInildareonsinnorthemareas,soastoimproveourknowedgeofvascularplaflt…     

Effect of mixture sowing on biomass allocation in the artificially-planted pastures,Southeastern Tibetan

Artificial planting is an important measure to promote the restoration of degraded grassland and protect the ecological environment. The objectives of the current study were to investigate the allocation pattern between aboveground biomass(AGB) and belowground biomass(BGB) in different seeding types of artificially-planted pastures. We explored the variation in biomass and the relationship between above-and belowground biomass in four artificiallyplanted pastures with one species from Elymus nutans Griseb(EN, perennial), Elymus sibiricus Linn(ES, perennial), Medicago sativa Linn(MS, perennial), and Avena sativa Linn(AS, annual) and in six artificially-planted communities with mixtures of two species by seeding ratio 1:1 from the abovementioned grasses(EN + AS, MS + AS, EN + ES, MS + EN, MS + ES, AS + ES) in 2015 and 2016. The results showed that E. nutans is the most productive species with the highest biomass production among the single crops. MS + ES was the most productive group in 2015, while the group with the highest biomass production changed to AS + ES in 2016. AGB was positively correlated to BGB in the surface soil layer in the first year, but positively related to BGB in the subsoil layer in the second year. In the early stageof artificial grassland succession, plants allocated more biomass to aboveground parts, with a root to shoot(R/S) ratio of 1.98. The slope of the log-log relationship between AGB and BGB was 1.07 in 2016, which is consistent with the isometric theory. Different sowing patterns strongly affected the accumulation and allocation of biomass in artificiallyplanted grassland, E. sibiricus was the suitable plant in the alpine regions, which will be conducive to understanding vegetation restoration and plant interactions in the future.     

基于浓缩法的浮游植物定量数据稳定性与可靠性分析

浮游植物是水生生态系统中重要的初级生产者,相对准确地定量它们的数量是进一步进行水质评价和生态功能分析的基础.通过采集处于不同营养状态的水库和不同处理的围隔中的浮游植物,研究影响浓缩法定量浮游植物的因素,了解如何通过浓缩法来合理地定量浮游植物.分析浓缩倍数、样品的显微镜计数量、水体营养状态对浮游植物丰度、生物量及群落多样性等定量参数稳定性的影响,同时比较单个不同水体中重复(或平行)样品之间浮游植物丰度的差别.结果表明,基于浮游植物的显微镜计数效率与定量数据稳定性的综合考虑,选择计数4片×10格/片即可基本保证定量数据的稳定性;在保证正常镜检的基础上,考虑水体营养状态适当增加浓缩倍数能够提高定量数据的可靠性;在特别依赖生物量或稀有种进行水质评价时,处于不同营养水平的水体均需要增加样品的平行数来提高定量数据的可靠性.     

The impact of wastewater treatment effluent on microbial biomasses and diversities in coastal sediment microcosms of Hangzhou Bay

Disposal of wastewater treatment plant (WWTP) effluent into sea, a typical anthropogenic disturbance, may influence many environmental factors and change the coastal microbial community structure. In this study, by setting up coastal sediment microcosms perturbed by WWTP effluent, the changes of microbial community structure under different degree of disturbances were investigated. Quantitative PCR (qPCR) and terminal restriction fragment length polymorphism (T-RFLP) were used to analyzed the biomass and biodiversity. High throughput sequencing analysis was used to identify the classification of the microorganisms. Our study suggested that low ratio of WWTP effluent may stimulate dominant species, which increase the biomass but decrease the biodiversity; while high ratio of WWTP effluent may depress all species, which decrease the biomass but increase the biodiversity. In other words, the impact was dose-dependent. The changes of microbial community structure may provide a metric for water environmental assessment and pollution control.     

新疆乌伦古湖浮游植物群落结构

于2006年11月至2007年7月按季度对乌伦古湖浮游植物进行了取样调查,共发现浮游藻类164种(包括若干未定名种),隶属于8门90属,其中Anabaena bergii为新疆新记录种,两种硅藻Chaetocerns sp.和Thalassionema sp.属于海产种类.各季种类组成均以绿藻门和硅藻门为主,蓝藻门和裸藻门次之,甲藻门、金藻门、黄藻门、隐藻门种类均比较少.秋季种类最多,有141种;其次是夏季和春季,分别为128种和121种;冬季种类最少,仅有95种.中华小尖头藻、微小四角藻、小形月牙藻、小球藻及尖针杆藻为全年可见优势种;肘状针杆藻在春季、夏季和秋季构成优势种群;扭曲蹄形藻和椭圆小球藻仅在夏季构成优势种群,而水溪绿球藻和粗刺四棘藻仅在冬季构成优势种群.藻类生物量周年变动模式为单峰型,生物量的峰值出现在夏季(6.77mg/L).最低值出现在冬季(1.45mg/L).结合历史资料分析发现,30年来,乌伦古湖浮游藻类不仅在种类组成上发生了明显的变化,在数量上也显著着一定的增长,并以绿藻和硅藻类群的增长最为显著.     

Study on spatial and temporal distribution of planktonic amphipoda in the East China Sea

Quantitative distribution of planktonic amphipoda in the East China Sea is analyzed and the relation with environmental factors and mackerel and scad fishing grounds is studied. The result shows that the characteristic of biomass is great seasonal and interannual variety during the passing three decades. The distribution of planktonic amphipoda basically reflects the movement, decay and intensification of the different water systems in the survey area.     

砷污染治理背景下阳宗海浮游植物生物量的时空分布模式及驱动因子

在社会经济发展和流域开发持续的背景下,砷污染已成为我国部分水体面临的重要环境问题,目前对砷污染防控的生态效应与修复效果评价仍缺乏系统识别。湖泊生态系统中浮游植物是重要的生产者,砷等重金属污染可以直接影响浮游植物生长、物种演替和初级生产力水平,浮游植物已成为指示砷污染水平及其生态效应的敏感指标。本研究以长期受到砷污染胁迫并经历污染治理的阳宗海为研究对象,设置南、中、北3个调查位点,于2015年4月-2019年12月对浮游植物和水质因子开展季节调查和空间分析,通过识别浮游植物生物量的时空分布模式与驱动因子,评价了砷污染与治理下浮游植物生物量的变化机制和生态修复效果。调查结果显示,采样期间阳宗海浮游植物以蓝藻门为主,浮游植物的生物量范围为0.7~30.4 mg/L,平均生物量在2016年最低((3.0±1.8) mg/L)、在2017年最高((10.5±8.9) mg/L)。ANOVA分析结果显示,浮游植物生物量存在显著的季节差异而空间差异不明显。相关分析结果显示,阳宗海浮游植物生物量与砷浓度和透明度呈显著负相关,而与水体温度和pH呈显著正相关。多元线性回归分析进一步显示,砷和水温是驱动阳宗海浮游植物生物量变化的显著环境因子。由此可见,在重金属污染湖泊经过修复后,水体砷遗留物的毒性效应仍然对浮游植物生长产生了明显的抑制作用,表明了水体重金属污染物可能具有长期的沉积物释放作用与持久的生态毒理效应。     

基于无人机和卫星遥感影像的升金湖草滩植被地上生物量反演

湿地植被地上生物量是衡量湿地生态系统健康状况的重要指标,对于珍稀水禽越冬繁殖、全球碳循环、生态净化具有重要意义,是生态学与遥感解译的研究热点之一.针对于地上生物量的测算,卫星遥感数据覆盖范围广但其空间分辨率较低,无人机遥感数据空间分辨率高但采集范围小,同时受湿地面积、观测系统及外界环境等条件的影响,使得遥感影像地上生物量反演更加复杂和困难.本研究基于无人机和高分一号数据对升金湖草滩植被地上生物量反演进行研究,结合升金湖保护区4个样区无人机可见光影像与相应样区实测样本数据,建立地上生物量与可见光波段、多种可见光植被指数的线性、幂函数、多项式、对数回归模型,并通过可决系数（R²）、平均绝对误差（MAE）和均方根误差（RMSE）对模型进行精度评价,选择最优模型对无人机影像进行地上生物量反演;通过可见光波段反演得到的生物量,与高分一号WFV归一化差分植被指数（Normalized Difference Vegetation Index,NDVI）影像相结合进行回归建模,获取整个升金湖草滩植被地上生物量分布.结果表明,利用无人机红光波段建立的多项式方程对地上生物量反演有着最高模拟精度,R²=0.86、预测精度MAE=111.33 g/m²、RMSE=145.42 g/m²,且红光波段生物量反演方法得到的结果与实际生物量分布一致性较高,高分一号WFV NDVI与无人机反演生物量构建的多项式模型为最优模型,R²为0.91.本研究利用无人机和高分一号数据进行生物量反演研究,整合多源遥感数据优点,以获取更加丰富和准确的信息,进而提高地上生物量反演精度,为湿地监测和湿地恢复管理提供数据和技术支撑,具有重要研究意义和应用价值.     

自然生境下盐角草的生物学特征及其影响因子

盐角草(Salicornia europaea L.)是一种典型的盐沼湿生植物,在盐渍重的地方常纯群分布,据报道是最耐盐的高等陆生植物之一。通过观测新疆境内3个区域5个典型种群的盐角草有关生物学参数及土壤水、盐等环境因子,发现:(1)随着土壤水分的减少,盖度减小,伴生种增多;随着盐分的加重,盖度减小,伴生种减少,成为单优势种;(2)不同生境下盐角草种群的密度变异很大,从190株/m2~6578株/m2不等。密度与株高、生物量、冠根比、分枝数及土壤水、盐等呈负相关,而与土壤容重显著正相关;(3)生物量与土壤含水量呈显著正相关(P<0.05),与土壤水溶性盐离子Na 、Cl-有较高的正相关,这两方面都是促进盐角草生长的主要土壤因素;(4)分枝数与土壤含水量及土壤水溶性Cl-含量显著正相关(p<0.05),说明高的土壤水、盐含量促进分枝;(5)盐角草植株的冠根比范围为13.75~85.39,大于一般农作物,是盐角草适应盐沼环境的显著特征之一。     

基于HJ-1A/B CCD影像的神农架巴山冷杉森林地上生物量估算

神农架林区气候复杂,森林类型多样,巴山冷杉林生境复杂,生物量估算难度较大。本研究首先将神农架林区植被细分类,提取出研究区巴山冷杉林分布范围,然后使用换算因子连续函数法对外业调查的研究区22块巴山冷杉样地进行样地生物量估算;最后利用逐步多元回归分析方法,估算巴山冷杉生物量。回归方程的拟合优度检验R值为0.851,决定系数R2为0.725,显著性检测值F为11.20。主要结论有:1)研究区提取出巴山冷杉面积为8 347.41 hm2,巴山冷杉生物量总量为102.51×104t,平均值为122.8 t/hm2。与前人的研究成果相比,估算平均值基本一致,实验结果可信。2)巴山冷杉林生物量出现了从边缘向内部的递减现象,巴山冷杉在林区呈现"离散化"分布,生物量也存在空间上的"退化"现象。