高光谱激光雷达提取植被生化组分垂直分布

对地高光谱激光雷达可以获得观测对象含有高光谱属性的全波形激光雷达回波,为探测植被生化特征的立体分布提供了新的遥感探测手段。基于此仪器开展室内试验,提出了植被生化组分垂直分布提取方法。首先,针对仪器的特点,提出了高光谱激光雷达全波形数据处理的方法;其次,以火炬花为例开展了室内扫描,并对获取的高光谱激光雷达数据进行了处理,获得带有高光谱属性的激光雷达点云数据;最后,根据植被指数与生化组分的关系,提取了叶绿素和胡萝卜素的生化组分垂直分布结果。研究结果表明,在植被顶部生化组分含量较低,叶绿素a普遍低于0.5 mg/g,胡萝卜素低于0.2 mg/g,而在中部叶片处,生化组分含量明显较高,与红色(顶部)和绿色叶片(中部)在植被垂直方向的分布一致,这表明基于仪器开展植被生理生化参数垂直分布遥感反演具有极大的应用潜力。     

The influence of environmental variability of a coastal lagoon ecosystem on genetic diversity and structure of white seabream [Diplodus sargus (Linnaeus 1758)] populations

As coastal lagoons serve as nursery areas for some marine and estuarine fish, selective pressures of these brackish or hypersaline lagoons may influence the genetic structure of species and populations. We examined spatial and temporal genetic patterns at eight microsatellite loci in white seabream [Diplodus sargus (Linnaeus 1758)] recruits from the Mar Menor (Southeast Spain) and compared these loci with those in coastal populations from the open sea, observing a high degree of genetic diversity and spatio‐temporal genetic stability. However, the results suggest the presence of subpopulations or genetic substructures in the Mar Menor D. sargus population that could be interpreted as a homogeneous mixture of individuals from three differentiated subpopulations in the Mediterranean and evidence of the Wahlund effect. It also suggests that D. sargus adults return to their original spawning habitat, thus conserving the genetic differences among the respective populations over time. Overall, this study demonstrates the importance of the Mar Menor coastal lagoon as a nursery area for the conservation of genetic diversity of D. sargus populations.     

Effect of copper on biochemical responses of the earthworm Eiseniafetida

Contamination sites pose significant environmental hazards to terrestrial ecosystems. Evaluation of the effects of contaminants in soils has become a priority for OECD countries. The biomarker approach is widely used both in vertebrates and in invertebrates for environmental biomonitoring. Eisenia fetida would provide diagnostic and prognostic early warning tests for monitoring soil contamination. These responses, often called early warning signals, would, in fact, give very important information when assessing the environmental risks of contamination. However, the use of biomarkers requires the identification of every possible variation that can influence the biochemical response, because ecosystems are generally subject to a mixture of pollutants,     

Starvation on changes in growth and fatty acid composition of juvenile red swamp crawfish, Procambarus clarkii

The effect of starvation on physiological and biochemical indicators of body, muscle and hepatopancreas were investigated for juvenile red swamp crawfish (Procambarus clarkii), and the results showed the effect was obvious. In a 70-day starvation experiment, mean body weight, exuvial rate, ab- dominal muscle ratio and hepatosomatic index decreased significantly. The percentage of protein, fat, ash and moisture were also significantly affected. The protein content decreased sharply at Day 30 onward and the percentage of fat decreased rapidly from Day 50 and on. The percentages of ash and moisture started to increase significantly at Day 40 and 30 respectively. After the starvation experiment, the hepa- topancreatic total and neutral lipid content decreased slightly from 23.90% to 20.39% and 73.75% to 70.35%, while the hepatopancreatic polar lipid content increased from 23.81% to 27.32%. On the other hand, all the muscular total, neutral and polar lipid contents, and the muscular fatty acid compositions did not change significantly except for the hepatopancreatic fatty acid composition. The relative percentages of 14:0, 16:0, 16:1n-7 and 18:1n-9 were decreased by 55.17%, 11.11%, 8.5% and 6.70% from the initial values, and the relative percentages of 18:2n-6, 18:3n-3, 20:4n-6, 20:5n-3 and 22:6n-3 increased obvi- ously from 5.2%, 3.9%, 2.4%, 10.6%, and 13.7% to 7.1%, 5.0%, 2.8%, 11.5%, and 14.4% respectively. Preference of utilization of fatty acids from hepatopancreas during starvation was as follows: 14:0>16:0>16:1n-7>18:1n-9, and the conservation of highly unsaturated fatty acids was in the order of: 18:2n-6 >18:3n-3>20:4n-6>20:5n-3>22:6n-3.     

Observations of individual humpback whales utilising multiple migratory destinations in the south-western Indian Ocean

Movements of humpback whales Megaptera novaeangliae among breeding regions within the southwestern Indian Ocean are poorly understood. Understanding the relationships among breeding regions is critical for effective conservation and management strategies. Through systematic comparisons of molecular genotypes and both systematic and non-systematic comparisons of individual identification photographs collected between 1996 and 2006, we have thus far identified nine whales (six males and three females) utilising two breeding areas within this region: the northern Mozambique Channel and eastern Madagascar. Four of the nine whales were recaptured using only photographic data, two whales were independently recaptured using both photographic and genetic data, and three whales were recaptured exclusively using molecular methods. The discovery of these nine individuals provides much-needed data to guide the formulation and future revision of stock boundaries.     

三种养殖模式下日本鳗鲡(Anguilla japonica)养成品血清生化指标和脏器消化酶、抗氧化酶活力的差异

随机选取池塘专养(M1)、日本沼虾套养(M2)以及水库放养(M3)三种养殖模式养成的肛长(25.91±3.26)cm的日本鳗鲡(Anguilla japonica)作为研究材料,采用生物化学方法测定并比较了不同养殖模式日本鳗鲡养成品血清生化指标和脏器消化酶与抗氧化酶活力。结果表明:(1)在所测16项血清生化指标中,三者间均无显著差异的为TP、ALB、GLB、ALT和CHE(P>0.05),均具显著差异的为CK、CREA和α-HBDH,它们的测定值依次呈M3>M2>M1(P<0.05)、M3>M1>M2(P<0.05)和M2>M1>M3(P<0.05);(2)所测消化酶中,三者均具显著差异的为肝脏蛋白酶、胃蛋白酶、肝脂肪酶及胰腺脂肪酶,它们的测定值均呈M1>M2>M3(P<0.05);(3)所测抗氧化酶中,三者间均无显著差异的为心脏SOD、CAT、POD和肝脏CAT活力(P>0.05),均具显著差异的仅为肝脏POD活力,其测定值呈M1>M3>M2(P<0.05)。     

Enzyme variation in marine and estuarine populations of a mud crab,Macrophthalmus hirtipes (Ocypodidae)

Protein and enzyme variation in 2 populations of the mud crab Macrophthalmus hirtipes (Jacquinot, 1853) (Ocypodidae) from 1 marine and 1 estuarine habitat were investigated by poly‐acrylamide gel electrophoresis. A survey of 24 loci revealed that 22 were common to both populations; 20 were monomorphic and 2 were highly polymorphic. Two alleles were detected for each of the polymorphic loci, esterase‐2 (EST‐2) and ester‐ase‐3 (EST‐3). The frequencies of the EST‐3 alleles were similar in the 2 populations. However, the frequencies of the 2 EST‐2 alleles in the estuarine population were significantly different from those for the marine population. Expression of 1 locus, esterase‐4 (EST‐4) was confined to the estuarine population. Two alkaline phosphatase loci (AKPH‐1 and AKPH‐2) were detected in the estuarine population, but only AKPH‐1 was found in the marine population. EST‐4 and AKPH‐2 were neither sex nor age specific. These interpopulational genetic differences may reflect differences in environmental conditions measured between the 2 habitats.     

PUBLICATIONS OF THE INTERNATIONAL ASSOCIATION OF HYDROLOGICAL SCIENCES

Abstract

Most commonly used biochemical oxygen demand (BOD) and dissolved oxygen (DO) models have been tested for their applicability in the River Kali, which is one of the most polluted rivers in India. A total of 732 field data sets were generated during field survey from March 1999 to February 2000. The modelling of BOD and DO in the River Kali involves derivation and solution of the governing equations that describe concentration change with time and space brought on by advective, decay, settling and loading functions. However, due to continuous discharges (e.g. from wastewater treatment plants) and steady-state flow conditions in the River Kali, the dispersion effects are found to be insignificant. In the analysis, the model parameters used in BOD-DO models were optimized using the Newton-Raphson technique and the performance of different models was evaluated using correlation statistics (r ²) and error estimation, viz. standard error (SE) and mean multiplicative error (MME). The results indicate that the BOD-DO models developed after Camp (1963) yielded the best agreement with the observed values as compared with several other approaches.     

Genetic improvement of New Zealand aquaculture species: programmes,progress and prospects

Aquaculture, like terrestrial farming, cannot achieve economic and sustainable production without high performing genetic stocks tailored to the conditions under which they are grown. It is essential, therefore, that aquaculture investment includes genetics and biotechnology to adapt marine livestock to the novel conditions of intensive aquaculture and to the demanding markets into which they are sold. The return on investment in well-structured breeding programmes can be very high, and significant performance and economic gains have been demonstrated in multiple species. Many factors must be considered in designing a genetic improvement programme, including the reproductive biology of the species and the identification of realistic and commercially relevant breeding goals based on the resources and facilities available. This paper reviews the options available to aquaculturists and provides examples of how these are being applied to six aquaculture species in New Zealand: king salmon, hāpuku, kingfish, Greenshell^TM mussels, Pacific oysters and pāua (abalone).     

基于归一化高光谱位置变量的干叶片生化组分分析

利用实测干叶片生化组分和高光谱反射率数据，提出了基于面积归一化的高光谱位置变量一阶导数极值法提取生化组分的新思路。结果表明：该方法能较好地提取叶片全氮、纤维素、木质素和淀粉含量，尤其是对纤维素、木质素和淀粉含量的反演精度有了提高。研究还表明：该方法在提取全氮、纤维素、木质素含量时，能有效剔除土壤影响，但是在提取淀粉含量时，效果不够理想。