褐藻酸降解菌侵染海带过程的超微结构观察

首次研究了褐藻酸降解菌侵入海带的途径，通过电镜观察，跟踪研究了褐藻酸降解菌染海带过程中海带表皮细胞壁的超微结构变化。结果表明，褐藻酸降解侵染海带的过程是首先引起海带表皮细胞壁藻胶层表面的破坏，然后引起藻胶层的断裂，并使藻胶层逐渐降解变突，最后褐藻酸降解菌通过海带细胞的壁藻胶层的断裂变突处进入海带细胞内。     

养殖对虾与海捕对虾鉴别方法初探

通过对养殖中国对虾(Penaeus orientalis)和海捕中国对虾肌肉煮熟后的失水率,提取物中的甜菜碱和氧化三甲胺含量,以及虾肉脂质中所含脂肪酸组成和含量进行了分析比较,得到下列结果:(1)两类对虾鲜样煮熟后失水率相差6％以上;(2)海捕对虾肌肉中的甜菜碱和氧化三甲胺含量比养殖对虾的高,两者有显著差别;(3)养殖对虾肌肉所含C18:2ω6为17.3±1.5％,海捕对虾为2.0±0.8％。从以上结果本文推荐以脂肪酸C18:2ω6含量来鉴别养殖对虾与海捕对虾。     

褐藻酸降解菌的产酶条件研究

以海带病烂处分离到的别单胞菌属（Alkteromonas)的褐藻酸降解菌菌株A1为研究对象，在该菌的产酶条件进行了研究。结果表明，产酶的最适温度20℃，褐藻酸钠浓度（质量分数）0．5％－0．6％，pH7.5，盐度15，氮源为（NH4)2SO4，培养时间72h。     

黑鲷三倍体的人工诱导研究

分别于1989年3—6月、1990年3—5月在天津和青岛两地采用冷、热休克法对黑鲷进行三倍体的人工诱导研究。结果表明,冷休克法诱导三倍体的效果好于热休克法,其最适诱导条件为卵子在受精后5min,温度为3—4℃的海水中处理10—20min,三倍体诱导率最高可达50.35％;正交试验证明,三倍体率受冷、热休克法的三因素影响程度的顺序由大到小依次为,处理时间、处理时刻、处理温度;黑鲷染色体数目:2n=48,3n=72,它们的染色体组型也同时获得。     

基于实例的GIS数据库模式匹配方法

针对空间数据库的特点,提出了一种改进的基于实例的层次式模式匹配方法,并解决了实体对应关系的自动建立、要素类相似性计算、属性相似性计算等问题.最后,通过实验验证了该方法的有效性.     

Fossil carotenoids and paleolimnology of meromictic Mahoney Lake,British Columbia,Canada

Vertical distribution of fossil carotenoids in a sediment core from meromictic Mahoney Lake was studied. Besides okenone and demethylated okenone, lutein and zeaxanthin and-carotene isomers were identified. No carotenoids typical for purple nonsulfur or green sulfur bacteria were detected. The ratio of zeaxanthin to lutein (above 1:1 in all samples) indicates a dominance of cyanobacteria over green algae in the phytoplankton assemblages of the past. Okenone, which is found exclusively in Chromatiaceae, was the dominating carotenoid in all sediment zones.The oldest sediment layers containing okenone were deposited 11 000 years ago. Between 9000 and 7000 and since 3000 years b.p., Chromatiaceae reached a considerable biomass in the lake. Vertical changes in okenone concentration were not related to changes of paleotemperatures. In contrast, okenone concentrations decreased during periods of volcanic ash input. During most of the lake history, however, mean okenone concentrations were positively correlated with sedimentation rates. This indicates that vertical changes of okenone concentration in the sediment reflect past changes of purple sulfur bacterial biomass in the lake.According to these results, the past limnology of Mahoney Lake resembled that of the present with a sulfide-containing monimolimnion and a well-developed population of okenone-bearing purple sulfur bacteria.     

地震短临综合预报方法的研究

本文在《中国震例》资料基础上,详细分析了华北地区１７次中强以上地震前的短临异常,得到华北地区中强以上地震短临异常的三个综合特征,在归纳出短临异常综合特征的基础上,提出了两个判定孕震过程、前兆异常由中期向短期过渡的定量的综合标志。据中期异常的追踪分析和短临异常综合分析相结合的原则,定义并计算了综合预报指标Ｓ值,以此值作为是否发生中强以上地震的判据。本文的重点是在分析短临异常综合特征的基础上,结合实用     

Geochemistry of porewater and sulfate-reducing bacteria in sediment cores from the Chianan Plain, Taiwan

High concentrations of arsenic and humic substances in groundwater from the southwestern coastal plain of Taiwan were well known for their probable relationships with black-foot disease. In order to realize the relationships between the concentrations of humic substances and arsenic in this area, 24 well water samples were analyzed. After filtered through 0.45 μm glass fiber membrane in the field, samples were kept in the dark at 4℃ and then separated into six fractions with varying range of molecular weight （〈0.5, 0.5-1, 1-5, 5-10, 10-50 and 〉50 k Da） by ultrafiltration apparatus （Molecular/Por Stirred Cell system） in the lab. Concentrations of humic substances were measured by fluorescence spectrometer （HITACHI F-2000, ex=370, em=445） and arsenic by FIAS-AA （Perkin Elmer AAnalyst 100, FIAS-400）. On average, only 6.2% of the total arsenic in water existed in the fraction of 〉0.5 k Da. and the others were complexed with humic substances （including humic acid and fulvic acid）. The results demonstrated a distinct positive correlation between the concentrations of humic substances and arsenic.     

XANES analysis of the mechanisms of arsenic removal in biological iron and manganese treatment unit

Biological iron and manganese removal utilizing indigenous iron and manganese oxidizing bacteria （IRB hereafter） in groundwater can also be applied to arsenic removal according to our pilot-scale test. The arsenic removal probably occurred through sorption and complexation of arsenic to iron and manganese oxides formed by enzymic action of IRB. We investigated the chemical properties of iron and manganese oxides in IRB floc and the valence state of arsenic sorbed to the floc to clarify the mechanisms of the arsenic [especially As （Ⅲ）] removal. The floc samples were collected from two drinking water plants using IRB （Jyoyo and Yamatokoriyama, Japan）, and our pilot - scale test site where arsenic and iron removal using IRB is under way （Mukoh, Japan）. The Jyoyo and Yamatokoriyama IRB floc samples were subjected to As （Ⅲ） and As（Ⅴ） sorption experiments. The elemental composition of the floc samples was measured. XANES spectra were collected on As, Fe and Mn K-edges at synchrotron radiation facility Spring 8 （Hyogo, Japan）. FT-IR and the X-ray diffraction spectra of the samples were also obtained. The IRB floc contained ca. 35 % Fe, 0.3%-3.5% Mn and 2%-6% P. The samples were highly amorphous and contained ferrihidrites and hydrated iron phosphate. According to XANES analyses of IRB, As associated with IRB was in ＋5 valence state when As （Ⅲ） （or As （Ⅴ）） was added in laboratory sorption test, Fe in ＋3 valence state, and Mn a mixture of＋3 and ＋4 valence states. Small shift was observed in the XANES spectra of IRB on As K-edge as the equilibration time of the sorption experiment was increased. Gradual oxidation of a small amount of As （Ⅲ） associated with IRB or change in arsenic binding with sorption site were the probable mechanism.     

Characterization of microbial communities associated with African desert dust and their implications for human and ecosystem health

Desert winds aerosolize several billion tons of soil-derived dust each year, including concentrated seasonal pulses from Africa and Asia. Huge dust events create an atmospheric bridge over continents and oceans, and eject a large pulse of soil-associated microorganisms into the atmosphere. These dust events might therefore have a role in expanding the biogeographical range of some microorganisms by facilitating rare long-distance dispersal events. The goal of this study is to characterize the microbes associated with African dust events and determine if they pose a risk to humans or downwind ecosystems. Air samples were collected by vacuum filtration in a source region （Mali, West Africa） during dust events and plated on R2A media to culture microorganisms. These organisms were compared to those in similar samples collected in the Caribbean during Saharan/Sahelian dust events. A high-volume liquid impinger is currently being tested in Barbados, collecting aerosol samples during African dust events. Over 100 bacteria and fungi （19 genera of bacteria and three genera of fungi） have been characterized from source region dust events.