Experimental study on a sampling technique based on a freeze-sediments valve for deep-sea microorganism

Deep-sea microorganism research has become a hot topic in life science, owing to its special value in high pressure and low temperature environment. If not kept the in-situ low temperature and high pressure, it will cause the microorganism to lose its activity and lead to a waste of resources. A freeze-sediments valve presents a promising solution to maintain the low temperature and high pressure in situ, improve the success rate of sampling and shorten the sampling time. A series of experiments were conducted to characterize the freeze-sediments valve, including the sampling time without leakage, adaptation to different sediments and the effect of the length, etc. Most important of all, temperature change of sediments is observed in all experiments. Experimental results indicated that the freeze-sediments valve was formed in 5 min without leakage and the freeze-sediments valve could be applied to different sediments without limitations on sediments type and mineral content. Considering the pressure retaining capacity and formation time of freeze-sediments valve, it was found that the freeze-sediments valve with a length of 60–80 mm has good pressure retaining capacity whose value was 41.3–48.4 Mpa and short formation time which was 5 min. Precooling the sampler could be used to shorten the sampling time in the actual environment application. This sampling technique can play a vital role in maintaining in-situ low temperature at −2 to −4 ° Celsius in all the experiments. According to the tests, the sampling technique based on the freeze-sediments valve had the potential to solve the problem of keeping in-situ temperature, low sampling success rate and long sampling time in the future.     

LED光色对欧洲舌齿鲈幼鱼抗氧化能力和消化能力的影响

作者利用循环水养殖实验系统,研究了红、绿、白、黄、蓝5种光色对欧洲舌齿鲈(Dicentrarchus labrax)幼鱼抗氧化能力和消化能力的影响。结果表明,红光组的欧洲舌齿鲈幼鱼超氧化物歧化酶(SOD)活力显著高于其他光色组(P0.05),而绿、白、黄和蓝光色组的SOD活力没有显著性差异;红光组的还原型谷胱甘肽(GSH)含量显著高于其他光色组(P0.05),绿、白光组与黄、蓝光组的GSH含量之间有显著性差异(P0.05);红光组过氧化氢酶(CAT)活力显著高于其他光色组(P0.05),白光组的CAT活力显著低于其他光色组(P0.05)。黄光组的胃蛋白酶活力显著高于红、绿、白光组(P0.05),绿光组的胃蛋白酶活力显著低于其他光色组(P0.05);红、绿、蓝光组的淀粉酶活力要显著高于白、黄光组(P0.05),红、绿、蓝光组的淀粉酶活力没有显著性差异,黄、白光组的淀粉酶活力没有显著性差异;白光组纤维素酶活力显著高于其他光色组(P0.05),绿光组的纤维素酶活力显著高于红、蓝光组(P0.05),黄光组的纤维素酶活力和绿、红、蓝光组没有显著性差异。因此,欧洲舌齿鲈在红光下养殖,其体内的抗氧化能力强,能有效应对氧化应激,而在黄光或红光下养殖其消化能力更强。     

轴压作用下双金属复合管组合作用与承载力分析

为探究轴压作用下双金属复合海底管道的组合作用与承载性能,对双金属复合海底管道进行了试验研究和理论分析。开展了不锈钢衬管材料性能试验,对比了国际主流不锈钢本构关系模型和试验结果。利用ABAQUS建立了精细化的双金属复合管道轴压试验有限元模型,系统研究了关键参数如复合工艺产生的环向复合应力、钢管初始缺陷幅值等对双金属复合管在轴压作用下力学性能的影响规律。通过对比已有轴压双金属复合管道试验结果,验证有限元模型。基于验证的有限元模型,对轴压作用下双金属复合管道的组合作用以及径厚比和材料强度对承载力的影响进行了分析。结果表明双金属复合管道的轴压极限承载力主要取决于基管的截面屈服荷载,并随着管径和材料强度的提高而增大。并依据分析结果对双金属复合海底管道的设计提出建议。     

考虑冲刷影响的筒型基础竖向极限承载力研究

大直径宽浅式筒型基础,阻水宽度大,在位工作期间受波浪海流作用,其周围土体易被冲刷。为研究单侧地基土体受冲刷后筒型基础的竖向极限承载力变化,通过引进冲刷率的概念,采用有限元方法研究了不同冲刷率下筒型基础的竖向极限承载力;并基于Meyerhof理论建立了计算不同冲刷率下筒型基础竖向极限承载力的极限平衡方法。研究结果表明,随着冲刷率增大,筒型基础的极限承载力出现不同程度的下降,当冲刷率为0.8时,即筒型基础单侧土体冲刷深度达6.4 m时,筒型基础的竖向极限承载力折减率为3.28%。建立的极限平衡算法可准确计算冲刷条件下筒型基础的竖向极限承载力。     

钙质土中重力串锚水平承载力特性有限元研究

目前没有规范给出串锚的水平承载力计算公式,为了利用有限元法研究串锚水平承载力特性,采用相关理论计算以及室内试验的方法来验证有限元法模拟计算的可靠性。实践证明,有限元法具有较高的计算精度。利用有限元分析软件ABAQUS建立了串锚-土体模型,模拟串锚在水平荷载作用下破坏过程,研究了串锚在水平荷载作用下破坏机理,从而得到了串锚水平承载力特性。在相同的工况条件下,串锚的水平承载力与其锚链长度有关,在一定范围内,增加锚链的长度可以提高串锚的水平承载力;串锚的水平承载力不是相应的单个锚的水平承载力相加,其水平承载力小于相应的单个锚水平承载力之和,在进行串锚水平承载力设计计算时应给与相应的考虑。     

Fuzzy comprehensive evaluation model for water resources carrying capacity in Tarim River Basin, Xinjiang, China

This paper explores the method of comprehensive evaluation of water resources carrying capacity and sets up an evaluation model applying the fuzzy comprehensive evaluation method. Based on the data of nature, society, economics and water resources of the Tarim River Basin in 2002, we evaluated the water resources carrying capacity of the basin by means of the model. The results show that the comprehensive grades are 0.438 and 0.454 for Aksu and Kashi prefectures respectively, where the current water resources exploitation and utilization has reached a relative high degree and there is only a very limited water carrying capacity, 0.620 for Kizilsu Kirgiz Autonomous Prefecture, where water resources carrying capacity is much higher, and in between for Hotan Prefecture and Bayingolin Mongolian Autonomous Prefecture. As a whole, the comprehensive grade of the Tarim River Basin is 0.508 and the current water resources exploitation and utilization has reached a relative high degree. Thus, we suggest that the integrated management of the water resources in the basin should be strengthened in order to utilize water resources scientifically and sustainably.     

The scavenging of free radical and oxygen species activities and hydration capacity of collagen hydrolysates from walleye pollock (Theragra chalcogramma) skin

Fish skin collagen hydrolysates (FSCH) were prepared from walleye pollock (Theragra chalcogramma) using a mixture of enzymes, namely trypsin and flavourzyme. The degree of hydrolysis of the skin collagen was 27.3%. FSCH was mainly composed of low-molecular-weight peptides and the relative proportion of <1000Da fraction was 70.6%. Free radical and oxygen species scavenging activities of FSCH were investigated in four model systems, including diphenylpicrylhy-drazyl radical (DPPH), superoxide anion radical, hydroxyl radical and hydrogen peroxide model, and compared with that of a native antioxidant, reduced glutathione (GSH). FSCH was also evaluated by water-absorbing and water-holding capacity. The results showed that FSCH was able to scavenge free radical and oxygen species significantly and to enhance water-absorbing and water-holding capacity remarkably. Therefore, FSCH may have potential applications in the medicine and food industries.     

基于空间数据挖掘技术的农用地产能核算数据库的应用研究

为提高农用地产能核算数据库的利用效率,以空间关联规则为基础,在河北省土地产能核算数据库的应用中得到提高河北省农业用地普查结果的利用效率的几条知识规则。利用现有软件,使用GIS软件进行空间数据预处理及空间关系计算。属性数据使用access管理,而关联规则挖掘算法直接应用Clementine。通过数据选取、数据预处理、数据变换、数据挖掘算法实施以及模式解释与评估,从空间数据库中提取用户感兴趣的空间模式与特征、空间与非空间数据的普遍关系及其他一些隐含在数据中的普遍的数据特征,从而得到提高土地产能核算成果利用效率的规律。     

Spatial partitioning between species of the phytoplankton-feeding guild on an estuarine intertidal sand flat and its implication on habitat carrying capacity

The fishery yield of Manila clams, Ruditapes philippinarum, increased considerably in the 1970s but has decreased rapidly since the middle 1980s on extensive intertidal sand flats in Ariake Sound (Kyushu, Japan). A survey conducted in 2004 on a 3.4-km² sand flat located in the central part of the Sound (Shirakawa sand flat) revealed four dominant species: two thalassinidean shrimps (Upogebia major and Nihonotrypaea japonica), which are deep-reaching burrow dwellers with strong bioturbation activities, and two bivalves (Mactra veneriformis and R. philippinarum). All four species belong to a phytoplankton (diatom)-feeding guild. In the late 1970s, the Manila clam population prevailed in high densities over the entire sand flat, whereas its distribution was restricted to the lowest quarter of the shore in 2004. In contrast, the population sizes and zones of occurrence of the other phytoplankton feeders have expanded in the absence of R. philippinarum, perhaps an indication of competitive release. After establishment, effects of the thalassinidean shrimps on sediment stability appear to have further reduced clam abundances. Across the sand flat in 2004, wet weight population biomass estimates for N. japonica, U. major, M. veneriformis, and R. philippinarum (whole body for shrimps and soft tissue for bivalves) were 304, 111, 378, and 234 tonnes, respectively. Based on Manila clam fishery yield records from Shirakawa, the carrying capacity of the Shirakawa sand flat in the late 1970s was estimated to be two times greater than the sum value for the whole phytoplankton-feeding guild in 2004. It is hypothesized that (1) the amount of phytoplankton determines the carrying capacity for the benthic community on the Shirakawa sand flat, with both phytoplankton and benthic biomass at maxima in the late 1970s, and (2) the subsequent increases in competition for space have caused further declines in the Manila clam population biomass to approximately one-eighth of its past value.     

Coastal environmental assessment and management by ecological simulation in Yeoja Bay, Korea

An eco-hydrodynamic model was used to estimate the carrying capacity of pollutant loads and response of water quality to environmental change in Yeoja Bay, Korea. An energy-system model also was used to simulate the fluctuation in nutrients and organic matter in the bordering wetland. Most water quality factors showed a pulsed pattern, and the concentrations of nutrients and organic matter of seawater increased when input loads of nutrients increased due to freshwater discharge. The well-developed tidal zones and wetlands in the northern area of the bay were highly sensitive to input loads. Residence times of water, chemical oxygen demand (COD), and dissolved inorganic nitrogen (DIN) within the bay were estimated to be about 16 days, 43.2 days, and 50.2 days, respectively. Water quality reacted more sensitively to the effects of nitrogen and phosphorus input than to COD. A plan to reduce the present levels of COD and dissolved inorganic phosphorus (DIP) by 20–30% and DIN by at least 50% in pollutant loads is needed for satisfying the target water quality criteria. The natural removal rate of nutrients in wetlands by reeds was assessed to be approximately 10%.