Non-specific immune response of bullfrog <Emphasis Type="Italic">Rana catesbeiana</Emphasis> to intraperitoneal injection of bacterium <Emphasis Type="Italic">Aeromonas hydrophila</Emphasis>

Non-specific immune response of bullfrog Rana catesbeiana to pathogenic Aeromonas hydrophila was studied to 60 individuals in two groups. Each bullfrog in bacterium-injected group was injected intraperitoneally （i.p.） with 0.2 ml bacterial suspension at a density of 5.2 × 10^6 CFU/ml, while each one in control group injected i.p. with 0.2 ml sterile saline solution （0.85%, w/v）. Three bullfrogs in both groups were sampled at 0, 1, 3, 7, 11, 15 and 20 days post-injection （dpi） for the evaluation of non-specific immune parameters. It was observed that intraperitoneal injection of A. hydrophila significantly increased the number of leucocytes and that of NBT-positive cells in peripheral blood. Significant increases in serum bactericidal activity and serum acid phosphatase activity were also observed in the bacterium-injected frogs when compared with those in the control group. However, a significant reduction was detected in vitro in phagocytosis activity of peripheral blood phagocytes. No significant difference in changes in the number of peripheral erythrocytes, serum superoxide dismutase （SOD） activity, and lysozyme activity was detected between the two groups. It is suggested that bullfrogs may produce a series of non-specific immune reactions in response to the A. hydrophila infection.     

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城市精明发展与生态效率的协同测度及交互响应——以中国276个地级以上城市为例

城市发展与生态环境存在复杂的动态交互耦合过程,厘清精明发展与生态效率的协同发展关系及交互响应机理,是区域可持续发展的关键环节。本文以2003—2015年中国276个地级以上城市为研究对象,采用多指标综合评价法、Super-SBM模型、灰色关联模型和面板VAR模型对中国城市精明发展与生态效率的协同发展和交互响应关系进行综合测度。研究表明：① 城市精明发展呈现从沿海向内陆阶梯递减的空间演变趋势,多个城市群具有较高的城市精明发展指数。② 生态效率与精明发展的空间变化具有一定的耦合特征,沿海地区南北方向呈现出较高水平的“生态弧线”城市带。③ 城市精明发展与生态效率具有显著的关联性特征,协同发展关系有下降趋势。④ 城市精明发展与生态效率存在双向交互的Granger因果关系,具有正向的交互响应关系,且存在一定的路径依赖特征和惯性发展趋势。⑤ 长期而言,城市精明发展对生态效率变动的影响更大。     

Examining the sensitivity of spatial scale in cellular automata Markov chain simulation of land use change

Understanding the spatial scale sensitivity of cellular automata is crucial for improving the accuracy of land use change simulation. We propose a framework based on a response surface method to comprehensively explore spatial scale sensitivity of the cellular automata Markov chain (CA-Markov) model, and present a hybrid evaluation model for expressing simulation accuracy that merges the strengths of the Kappa coefficient and of Contagion index. Three Landsat-Thematic Mapper remote sensing images of Wuhan in 1987, 1996, and 2005 were used to extract land use information. The results demonstrate that the spatial scale sensitivity of the CA-Markov model resulting from individual components and their combinations are both worthy of attention. The utility of our proposed hybrid evaluation model and response surface method to investigate the sensitivity has proven to be more accurate than the single Kappa coefficient method and more efficient than traditional methods. The findings also show that the CA-Markov model is more sensitive to neighborhood size than to cell size or neighborhood type considering individual component effects. Particularly, the bilateral and trilateral interactions between neighborhood and cell size result in a more remarkable scale effect than that of a single cell size.     

Dynamic modeling and vibration suppression for an offshore wind turbine with a tuned mass damper in floating platform

The worldwide demand for renewable energy is increasing rapidly. Wind energy appears as a good solution to copy with the energy shortage situation. In recent years, offshore wind energy has become an attractive option due to the increasing development of the multitudinous offshore wind turbines. Because of the unstable vibration for the barge-type offshore wind turbine in various maritime conditions, an ameliorative method incorporating a tuned mass damper (TMD) in offshore wind turbine platform is proposed to demonstrate the improvement of the structural dynamic performance in this investigation. The Lagrange's equations are applied to establish a limited degree-of-freedom (DOF) mathematical model for the barge-type offshore wind turbine. The objective function is defined as the suppression rate of the standard deviation for the tower top deflection due to the fact that the tower top deflection is essential to the tower bottom fatigue loads, then frequency tuning method and genetic algorithm (GA) are employed respectively to obtain the globally optimum TMD design parameters using this objective function. Numerical simulations based on FAST have been carried out in typical load cases in order to evaluate the effect of the passive control system. The need to prevent the platform mass increasing obviously has become apparent due to the installation of a heavy TMD in the barge-type platform. In this case, partial ballast is substituted for the equal mass of the tuned mass damper, and then the vibration mitigation is simulated in five typical load cases. The results show that the passive control can improve the dynamic responses of the barge-type wind turbine by placing a TMD in the floating platform. Through replacing partial ballast with a uniform mass of the tuned mass damper, a significant reduction of the dynamic response is also observed in simulation results for the barge-type floating structure.     

Hydrodynamic performance of a T-shaped floating breakwater

The comprehensive utilization of floating breakwaters, specially acting as a supporting structure for offshore marine renewable energy explorations, has received more and more attention recently. Based on linear water-wave theory, the hydrodynamic performance of a T-shaped floating breakwater is semi-analytically investigated through the matched eigenfunction expansion method (MEEM). Auxiliary functions, to speed up the convergence and improve the accuracy in the numerical computations, are introduced to represent the singular behavior of fluid field near the lower salient corners of the structure. The effects of the height and installation position of the vertical screen on the reflection and transmission coefficients, dynamic response and wave forces are examined. It is found that the presence of the screen shifts the resonance frequency of RAO for both surge and pitch modes to the low-frequency area, while has no effect on heave mode. The identical added masses, damping and transmission coefficients can be obtained in the cases where the screen holds the same distance away from the longitudinal central axis of the upper box-type structure. Moreover, a relatively small pitch response can be achieved in a wide wave–frequency range, when the breakwater is Γ-shaped.     

Effects of wave directionality on extreme response for a long end-anchored floating bridge

Reliable design codes are of great importance when constructing new civil engineering concepts such as floating bridges. Previously only a scarce number of floating bridges have been built in rough wave conditions and only limited knowledge of the extreme environmental conditions and the associated extreme response exists. To form a better design basis an increased understanding of the sensitivity in the structural response towards changes in short-crested sea parameters is needed. Furthermore, acquiring the necessary accuracy in simulated extreme response is often a computationally expensive endeavour and the number of simulations needed is often based on experience. The present study investigates the wave-induced short-term extreme response of a simplified end-anchored floating bridge concept for several wave environments with a return period of 100 years. The study includes convergence of the coefficient of variation for the extreme response for different realization lengths as well as number of realizations. The sensitivity in the structural response towards different main wave directions and spreading exponents is investigated and includes both transverse and vertical displacement response spectra and extreme Von Mises stress in the bridge girder cross-section. The extreme response is based on an accuracy of 2% in the coefficient of variation equivalent to 40 3-h realizations and a low sensitivity in the response is found for natural occurring spreading exponents and for main wave directions within 15° from beam sea.     

Isolation, distribution and evaluation of cytotoxic and antioxidant activity of cultivable actinobacteria from the Oman Sea sediments

Screening bioactive natural products from bacteria is a determinative step in the drug discovery programs. The present study aim to isolate actinobacteria from the Oman Sea sediments for determining the effects of different culture media and treatments on the yield of the isolation process, and measure the DPPH radical scavenging and Artemia cytotoxic activity of culture extracts of the actinobacterial isolates. A total of 290 actinobacterial isolates were collected from 14 sediment samples. Heat treatment(40.68%) and M_4 medium(29.31%) exhibited the maximum isolation rates of actinobacteria. Streptomyces isolates were dominantly distributed in all of the investigated stations according to 16 S rRNA gene sequencing. The distribution pattern of Streptomyces followed a depth-dependent frequency trend, whereas the members of rare genera including Micromonospora, Nocardia Actinoplanes, Nocardiopsis, Saccharopolyspora and Crossiella were distributed in deeper stations. Approximately,25% of the examined isolates could scavenge 90% of 10~(–4) mol/L DPPH solutions at 1 250 μg/mL final concentration of their ethyl acetate culture extracts. Furthermore, the most potent extracts could scavenge DPPH radicals with IC50 ranges from 356.8 to 566.4 μg/mL. Brine shrimp cytotoxicity tests showed that 38.88% of the examined culture extracts exhibited LC_(50) lower than 1 000 μg/mL against the Artemia cells. Moreover, the most potent culture extracts exhibited LC_(50) range from 335.4 to 534.4 μg/mL. Phylogenetic analysis by 16 S rRNA gene sequence revealed that the OS 005, OS 263 and OS 157 closely related to Streptomyces djakartensis, Streptomyces olivaceus and Nocardiopsis dassonvillei respectively. These results suggested the widespread distribution of the antioxidant and cytotoxic producing actinobacteria in the Oman Sea sediments, which could be considered as promising candidates for the discovery of microbial bioactive compounds.     

天然气水合物赋存区甲烷渗漏活动的地球化学响应特征

综述了天然气水合物赋存区甲烷渗漏活动的地球化学响应指标的研究进展,分析了应用单一指标识别甲烷渗漏活动各自所存在的问题,包括浅表层沉积物孔隙水中CH_4、SO_4~(2–)、Cl~–等离子浓度随深度的变化;浅层沉积物全岩W_(TOC)(W表示质量分数,TOC表示总有机碳)和W_(TS)(TS表示总硫)之间的相关性及比值;自生碳酸盐岩δ~(13)C和δ~(18)O;自生矿物重晶石、黄铁矿、自生石膏的δ~(34)S;有孔虫壳体和生物标志化合物的δ~(13)C等。结果表明孔隙水中的CH_4、SO4_~(2–)浓度及溶解无机碳的碳同位素组成可以用来识别目前正在发生的甲烷渗漏活动;而沉积物中的WTS、自生矿物的δ~(34)S、钡含量及其异常峰值和生物标志化合物的δ~(13)C等指标的联合使用可以更真实准确地反映地质历史时期天然气水合物赋存区的甲烷渗漏活动。因此,在实际研究过程中,可将孔隙水和沉积物两种介质的多种指标相结合。随着非传统稳定同位素(Fe、Ca、Mg等)和沉积物氧化还原敏感元素(Mo、V、U等)等研究的发展,甲烷渗漏活动地球化学响应指标的研究也将得到拓展,而多种地球化学指标的联合使用将为天然气水合物勘探及其形成分解过程识别研究提供重要的科学依据。     

海萝抗氧化酶系与失水响应因子表达模式分析

潮间带红藻海萝(Gloiopeltis furcata)对失水胁迫具有很强的耐受能力。为探索海萝周期性失水过程中的响应机制,本研究在24 h内设计了两次连续的失水-复水循环处理,测定了海萝抗氧化酶活力的变化情况。同时,利用转录组测序技术,结合荧光定量PCR方法(qRT-RCR),对海萝失水响应基因的转录表达进行了验证。结果表明:海萝转录组共组装到32681条基因。与对照组相比,处理组共表达了7161条差异表达基因(differentially expressed genes, DEGs)。KEGG分析显示, DEGs分布在代谢、环境信息加工、有机体系统、遗传信息加工、细胞进程等方面。海萝抗氧化酶活力测定发现,抗氧化能力对海萝响应失水胁迫十分重要。过氧化氢酶(CAT)、硫氧还蛋白还原酶(TrxR)、超氧化物歧化酶(SOD)参与抗氧化过程,其中CAT酶活力对海萝抵抗失水胁迫尤为重要。此外,qRT-PCR结果显示,海萝中渗透调节物质红藻糖苷合成的相关基因(GfUGPase、GfGK、GfGPDH)只对初次失水处理有正响应。而热激蛋白70基因(GfHSP70)、碳酸酐酶基因(GfCA)、MYB蛋白编码基因(GfMYB)以及谷胱甘肽S转移酶基因(GfGST)在两次失水过程中其转录水平均有上调表达,它们也参与了海萝失水响应机制。