Preparation,Characterization and Pharmacokinetics of Fluorescence Labeled Propylene Glycol Alginate Sodium Sulfate

A rapid and sensitive fluorescence labeling method was developed and validated for the microanalysis of a sulfated polysaccharide drug,namely propylene glycol alginate sodium sulfate （PSS）,in rat plasma.Fluorescein isothiocyanate （FITC） was selected to label PSS,and 1,6-diaminohexane was used to link PSS and FITC in order to prepare FITC-labeled PSS （F-PSS） through a reductive amination reaction.F-PSS was identified by UV-Vis,FT-IR and ^1H-NMR spectrum.The cell stability and cytotoxicity of F-PSS were tested in Madin-Darby canine kidney （MDCK） cells.The results indicated that the labeling efficiency of F-PSS was 0.522%±0.0248% and the absolute bioavailability was 8.39%.F-PSS was stable in MDCK cells without obvious cytotoxicity.The method was sensitive and reliable; it showed a good linearity,precision,recovery and stability.The FITC labeling method can be applied to investigating the absorption and metabolism of PSS and other polysaccharides in biological samples.     

一株高效褐藻酸降解菌的筛选、鉴定及其发酵条件的优化

以褐藻酸钠为唯一碳源,从腐烂海带中筛选得到褐藻酸钠降解能力较强的菌株H4,经生理生化和16S rDNA序列分析鉴定该菌株属于交替单胞菌属(Alteromonas)。对菌株H4的发酵条件优化研究表明,H4的较优培养基组成为(w/v):褐藻酸钠0.6%、酵母粉0.5%、蛋白胨0.25%、NaCl 3%;较优培养条件为:培养温度28℃,初始pH7.5,接种量1.5%(v/v),培养时间48 h。Fe3+对交替单胞菌H4的产酶有明显的促进作用,这在其他褐藻酸裂解酶生产菌株中未见报道,而Cu2+对褐藻酸裂解酶的抑制作用高达45.78%。在优化后的培养条件下,粗酶液酶活达到146.45 U/mL,较优化前提高了39.4%。     

Optimized water vapor permeability of sodium alginate films using response surface methodology

The water vapor permeability （WVP） of films is important when developing pharmaceutical applications. Films are frequently used as coatings, and as such directly influence the quality of the medicine. The optimization of processing conditions for sodium alginate films was investigated using response surface methodology. Single-factor tests and Box-Behnken experimental design were employed. WVP was selected as the response variable, and the operating parameters for the single-factor tests were sodium alginate concentration, carboxymethyl cellulose （CMC） concentration and CaClz solution immersion time. The coefficient of determination （R2） was 0.97, indicating statistical significance. A minimal WVP of 0.389 8 g-mm/（m^2.h.kPa） was achieved under the optimum conditions. These were found to be a sodium alginate concentration, CMC concentration and CaCl2 solution immersion time at 8.04%, 0.13%, and 12 min, respectively. This provides a reference for potential applications in manufacturing film-coated hard capsule shells.     

褐藻酸降解菌A7的发酵及产酶条件研究

以海藻废弃物堆肥中分离到的薄壁杆菌属（Gracillibacillus）的褐藻酸降解菌A7为研究对象,对该菌的生长及产酶条件进行了研究。结果表明,该菌的最适产酶条件为：温度30℃,海藻酸钠的质量分数0．5％,pH9．5,NaCl浓度0．5mol／L,以蛋白胨为主要氮源。在最佳条件下培养96h达到最高酶活力12．79U／mL。     

褐藻酸钠降解工艺的研究

初步研究褐藻酸钠在盐酸乙醇溶液中的部分降解过程。实验研究水浴温度，乙醇浓度，盐酸浓度和降解时间对褐藻酸钠降解的影响。指出：在其它条件相同的情况下，水浴温度、盐酸浓度和水解时间呈正相关，乙醇具有促进盐酸降解褐藻酸钠的作用，并表明乙醇浓度与降解速度呈负相关，指出适宜的乙醇用量为５％～１０％，同时还得到了制备不同粘度褐藻酸钠的适宜条件。     

Vibrio sp.510产褐藻胶裂合酶的底物专一性分析

Vibrio sp.51 0具有很强的产生褐藻胶裂合酶的能力。本实验对该菌发酵产生的褐藻胶裂合酶经疏水色谱除去杂蛋白 ,再经灌注色谱分离得到 3个酶组分峰。经底物专一性检测 ,峰 1和峰 3对聚甘露糖醛酸和聚古罗糖醛酸均具降解作用 ,峰 2只对聚甘露糖醛酸有降解作用。园二色谱测定酶的二级结构 :峰 1结构最为复杂 ,以β-转角为最高 ,占 31 .5% ,无规线团占 2 7% ,α-螺旋占 2 5.8% ;峰2为β-折叠 ,占 95.5% ;峰 3以 60 %的α-螺旋和 40 %的无规线团形式存在。 3个分离峰的底物专一性和二级结构的差异证明了褐藻胶裂合酶蛋白的结构与功能之间的相关性。     

Effects of composition and structure of alginates on adsorption of divalent metals

Results of a series of experiments (on the adsorption of divalent metal ions by dried alginic acid, Na and Ca alginates of different composition and block structure) conducted in this systematic study of the effects of the composition and structure of alginates on the static adsorption equilibrium of divalent metal ions indicate that the properties of alginate adsorption to divalent metal ions are highly different, depending not only on the cations used, but also on the form and structure of the alginates. There is close correlation between the adsorption properties and the structure of the alginates. The selectivity coefficient of Na alginate for Cd-Sr ion exchange tends to increase with the increase of theM/G ratio in alginate, whereas the adsorption capacity of Ca alginate for Cu²⁺ ion decrease with the increase of the G-block or the average length of the G-block and the total adsorption capacity of alginic acid is found to vary in the same order as theF _MM(diad frequency) in alginate in the mixed solution of Sr²⁺, Ba²⁺ and Cd²⁺. Contribution No. 2166 from the Institute of Oceanology, Chinese Academy of Sciences.     

褐藻胶裂解酶产生菌Vibro sp．OY102的发酵条件优化

对海洋来源的Vibro sp．QY102的产褐藻胶裂解酶的发酵条件进行研究。结果表明，该菌株最适液体培养基成分为（w／v）：0．5％褐藻酸钠；0．4％蛋白胨；0．3％KH2PO4；0．7％K2HPO4·3H2O；2％NaCl；0．01％MgSO4·7H2O，pH=6．0。按3％的接种量接入培养基，30℃150r／min振荡培养120h，产酶达到10．2u／mL，为优化前的4．5倍。Mg^2＋是该菌株产酶所必需的，这在其他褐藻胶裂解酶生产菌株中未见报道。该菌株产酶发酵条件的研究，为褐藻胶裂解酶的大规模制备及应用奠定了基础。     

Effects of alginate gel on rheological properties of hair-tail (Trichiurus lepturus) surimi

Effects of alginate gel at different concentrations on rheological properties of hair-tail (Trichiurus lepturus) surimi were investigated. Alginate gel (1% - 3%) exhibited enhanced effects, especially when alginate gel concentration increased. The rheological properties of mixture samples were studied by the time sweep, frequency sweep and temperature sweep. The critical strain values of the mixture samples for the onset of non-linear viscoelasticity were about 5%. The storage modulus G’ of the mixture samples increased with time for 4 h. The frequency sweep showed that G’ was greater than G" for all the mixture gels with different alginate gel concentrations, and values of both n’ and n" for all samples were low (<0.2), these constants corresponding to G’ and G", and indicating the elasticity of mixture gels. The values of storage modulus G’ decreased during heating process and increased with decreasing temperature.     

两种不同海洋生物材料对大鼠急性创面修复效果的研究

为研究来源于海洋的鱼类胶原蛋白和海藻酸盐对创面的修复效果,建立大鼠皮肤急性创面模型,对两种材料的功效进行探索。研究采用临床常用创面敷料使用方法,将两种材料植入创面处,采用细胞生物学、病理学等方法进行观察评价。结果显示,鱼胶原蛋白和海藻酸盐均具有良好的生物相容性和诱导创面修复能力,但在诱导血管长入、材料降解融合以及整体创面修复状态等方面,鱼类胶原蛋白要优于海藻酸盐。结果证明,两种不同的海洋生物材料均可以创造良好的伤口愈合环境,有效促进创面的修复。