不同类型的高位虾池排污对秋茄幼苗生长的影响

中国多数红树林陆地边缘存在着大量的养虾池塘。这些养虾池塘可通过：（1）换塘外排废水,（2）清塘直排污泥影响周围的红树林。本研究共包含两个实验,来探讨这两种类型的排污对秋茄幼苗生长的影响。实验一模拟虾池常规换塘情况,研究换塘废水对秋茄幼苗生长的影响。实验二设置四种虾池清塘污泥沉积厚度（0 cm,2 cm,4 cm,8 cm）,研究虾池清塘污泥的累积效应对秋茄幼苗生长的影响。结果如下：在5,15和25三种盐度下,虾池换塘废水未对秋茄生长指标造成显著影响;而清塘污泥对秋茄生长的影响与其沉积厚度有关,大多数生长参数在污泥沉积厚度为4 cm时呈现最大值。秋茄幼苗在适量的沉积厚度下加速了生长,且本实验秋茄幼苗对虾池清塘污泥的耐受性为沉积厚度8 cm。     

浙南近海虾类群落结构及其多样性分析

作者根据2015年11月(秋季)、2016年2月(冬季)、2016年5月(春季)和2016年8月(夏季)对浙南近海进行的渔业资源调查数据,用生物量作为虾类资源分布的数量指标,对该海域虾类的组成、数量分布以及季节变化进行分析。结果显示:该区域共鉴定出虾类30种,隶属于10科21属,以对虾科(Penaeidae)虾类最多,其次为管鞭虾科(Solenoceridae),长臂虾科(Palaemonida)位居第3位。按季节来看,秋季共鉴定虾类27种,各站位平均生物量为6.97 kg/km2,优势种为凹陷管鞭虾(Solenocera koelbeli)、中国毛虾(Acetes chinensis)和中华管鞭虾(Solenocera crassicornis)等3种;冬季鉴定出虾类25种,平均生物量为1.65 kg/km2,优势种为凹陷管鞭虾、扁足异对虾(Atypopenaeus stenodactylus)、日本鼓虾(Alpheus japonicus)、鲜明鼓虾(Alpheus digitalis)、中国毛虾、中华管鞭虾和周氏新对虾(Metapenaeus joyneri)等7种;春季20种,平均生物量为2.22 kg/km2,优势种为戴氏赤虾(Metapenaeopsis dalei)、东海红虾(Metapenaeopsis dalei)和中华管鞭虾等3种;夏季16种,平均生物量为10.36 kg/km2,优势种有鹰爪虾(Trachysalambria curvirostris)和中华管鞭虾等2种。Margalef丰富度指数(D)、Shannon-Wiener多样性指数(H′)和Pielou均匀度指数(J′)秋季均低于冬季。秋冬季受到浙闽沿岸流影响,温度、盐度相对较低,夏季受到台湾暖流外侧影响,温度、盐度相对较高,且各季节间由于瓯江、椒江、飞云江、鳌江等陆源性冲淡水的影响,盐度、温度变化相对较大,使得该区域的物种不得不适应一个跨度较大的温盐范围,广温广盐性种类为主。     

Heterosis and heritability estimates for the survival of the Pacific white shrimp (Litopenaeus vannamei) under the commercial scale ponds

The aim of the present study is to detect the potential of the base population from diallel crosses of eight introduced strains of the Pacific white shrimp(Litopenaeus vannamei) for improving the yield. Heterosis and heritability were estimated for pond survival at commercial farm conditions for the base population that included 207 full-sib families from a nested mating design by artificial insemination. Among all the hybrids,the heterosis ranged from –11.37%(UA1×UA2) to 20.53%(UA3×SIN) with an average of 0.953%. The results showed that more than half of the hybrids(51.85%) have negative heterosis for survival rate, but most of the hybrids with positive heterosis have high estimates. The high proportion of negative heterosis for survival rate reminders us that the survival trait also should be considered in the crossbreeding program to avoid yield decrease. However, high positive heterosis manifested in most of the hybrids for survival indicates the usefulness of these hybrids for improving the survival to obtain higher yield by crossbreeding in this breeding program. The heritability estimate for pond survival was 0.092±0.043 when genetic groups were included in the pedigree, and it was significantly different from zero(P0.05). The results from this study also indicated that significant improvement for survival is possible through selection in L. vannamei.     

Genetic variability of the blue and red shrimp Aristeus antennatus in the Western Mediterranean Sea inferred by DNA microsatellite loci

Genetic variation at eight microsatellite loci was studied in nine populations of the blue and red shrimp Aristeus antennatus to investigate whether distinct stocks are present in the Western Mediterranean Sea. A high level of gene flow and no evidence of genetic partitioning were discovered. No significant variation was found (F_ST = 0.00673, P-value = 0.067) even when shrimps from exploited and those from deep-water unexploited grounds were compared. No evidence of reduction or expansion of population size in the recent past was found, as indicated by the bottleneck and interlocus g-tests. Our results are consistent with previous studies using mitochondrial gene methods and allozymes, indicating that, for this species, extensive pelagic larval dispersal and adult migration are probably responsible for the genetic homogeneity observed. In particular, due to a different bathymetric distribution of males and females, reported to be associated with different water masses and hence with possible differential dispersal capacity between sexes, the hypothesis of sex-biased dispersal was tested. Mean values of corrected assignment indices and mean relatedness values were higher for males, suggesting that females are the more widely dispersing sex. Molecular assessment of A. antennatus from the Western Mediterranean provides data of biological and evolutionary interest for the successful management of such a highly valuable fishery resource.     

Atypical size selection of captive school prawns,Metapenaeus macleayi,by three recreational fishing gears in south‐eastern Australia

Three manipulative experiments were done to estimate the selectivity of conventional and new sizes and configurations of mesh for school prawns, Metapenaeus macleayi, in three south‐eastern Australian recreational fishing gears (haul, push, and scoop nets). The treatment meshes examined were: (1) conventional‐sized, diamond‐shaped mesh used in all gears (20 mm in scoop nets and 30 mm in push and haul nets); (2) 30 mm in scoop nets; and (3) 40‐mm diamond‐ and (4) 23‐mm square‐shaped mesh in all gears. In all experiments, known quantities of school prawns (6–24 mm carapace length (CL)) were placed in purpose‐built enclosures, monitored to ensure no experimental‐induced stress (as measured by changes in L‐lactate in their haemolymph) and the replicate treatments of the various gear configurations deployed. Escapees from the various treatment nets were collected from the enclosures using fine‐meshed nets. Logistic selection curves were derived for all treatment nets and specific comparisons made within and among gears. All nets had 50% retention lengths (L₅₀) comparable to other penaeid‐catching gears with similar mesh sizes, but most had selection ranges (SRs) that were atypically inflated. The large SRs were attributed to a combination of factors that included the mesh geometry and towing speed of the gears and the behaviour of school prawns. The 20‐mm scoop net had the smallest selection parameters, retaining >99% of individuals larger than 13 mm CL. Mesh size in this gear would need to be increased to at least 30 mm to allow some maturing prawns (>18 mm CL) to escape.     

海黍子(Sargassum muticum)池塘养殖及对氮磷去除效果的研究

初步研究了在海参养殖池塘中,不同养殖方式下,大型海藻海黍子(Sargassum muticum)的生长特性及对水体中氨氮和活性磷的去除作用。2012年3~5月,在山东莱州试验养殖海黍子60d,结果发现其长度最大从43.8cm增长到81.3cm,增长85.6%,日增长率(SGR)在14℃时最大,达到5.0%;每绳的平均质量从0.74kg增到2.59kg,增长3.5倍,SGR在12℃时最大,达4.3%;水体氨氮平均下降了25.9%,活性磷下降了37.5%。实验证明海黍子能在适合的池塘中养殖,并且能有效的去除水体中的氨氮和活性磷,是有效的近海生境修复大型工具种海藻。     

尾矿库选址勘察渗漏性评价方法探讨

文中阐述了尾矿库选址中的工程地质和水文地质测绘、浅层地震和勘察工程、水文地质试验等对库区渗漏性进行评价的基本方法,并结合工程实例介绍实际工作中如何进行渗漏性评价及重点关注的问题。     

Influence of Ultrasonic Treatment on the Allergenic Properties of Shrimp （Penaeus vannamei ） Allergen

The present study was undertaken to determine whether high intensity ultrasound could reduce the allergic properties of shrimp allergens. Reducing the allergenic properties of these allergens will be beneficial to allergic individuals. Samples of shrimp protein extract and shrimp muscle were treated by high-intensity ultrasound with water bathing at 0 ℃ or 50 ℃ for different time periods. The treated and untreated samples were then analyzed by SDS-PAGE, Western blots and competitive inhibition ELISA （Ci-ELISA） to determine the shrimp allergenicity. The results show that high-intensity ultrasound has no effect on allergenicity when the extracts were treated at 0℃. However, a significant decrease was observed in the level of the major shrimp allergen, Pen a 1, when the samples were treated at 50 ℃. In the determination of allergenicity with CiELISA, a reduction in IgE binding was also observed.