鲶爱德华氏菌间接酶联免疫快速检测法的建立

用鲶爱德华氏菌兔抗血清作为一抗,碱性磷酸酶(AP)标记的羊抗兔IgG作为酶标二抗,建立黄颡鱼"红头病"病原菌—鲶爱德华氏菌的间接酶联免疫(ELISA)快速检测法,并优化检测条件。抗原最佳包被浓度为107/mL,一抗工作的最佳稀释度为1∶211,病原菌的检测灵敏度为105/mL,交叉反应实验证明该方法特异性强,与迟钝爱德华氏菌、弧菌等13种标准菌株无交叉。应用上述技术对人工感染发病鱼中分离的优势菌进行检测,结果表明阳性检出率为80%;对自然发病黄颡鱼体内分离获得的20株优势菌检测结果表明,12株菌为鲶爱德华氏菌。     

企业社区居民地方认同变化及作用机理——以西北第一印染厂社区为例

地方认同是衡量人地情感、理解人与地方认知关系的重要概念,能够反映人地互动的复杂动态关系。企业社区作为重要的城市居住空间,理解居民地方认同对于科学开展城市社区更新、街区振兴与可持续发展具有重要意义。以西安市西北第一印染厂社区为例,采用扎根理论方法,分析社会环境变迁和社区重构影响下,居民个人身份与地方认同的变化及作用机理。研究发现：1）企业社区居民个人身份认同是从单位人向社会人,以及未来作为留守老年群体的转变。2）延续传承的集体记忆维系居民积极式认同,社会交往生疏化和社区管理社会化加剧地方认同弱化,破败的建筑环境和落后的配套设施带来消极式认同。3）居民在生活、成长的企业社区环境和社会互动中构建了自身及对地方的认同,随着外部社会环境与内部居民个体特征而变化。外部社会环境的国企改革、住房改革使企业转轨、社区社会空间转变,加以居住时间差异、个体特征、心理因素与个人经历变化等内部因素,共同塑造居民的地方认同及其变化过程。     

MODIS干旱指数对华北干旱的敏感性分析

针对近年来干旱发生频繁的华北地区,通过利用2000-2009年MODIS数据和气象观测站降水资料,建立草地和农田距平植被指数(AVI)与不同时间尺度标准化降水指数(SPI)之间的相关统计模型,比较AVI和距平水分指数(AWI)对干旱响应的敏感性。结果表明：(1)植被生长季AVI与不同时间尺度SPI的相关关系不同。草地AVI与1个月尺度的SPI(即1-SPI)相关系数较低,而与3-SPI相关最显著;而农田区AVI与SPI的相关性较低,总体上农田AVI与3-SPI的相关性较高;(2) AWI与AVI类似,也对SPI存在时滞响应,均与3-SPI有着极显著的相关关系,并且在干旱发生较严重的6~8月份AWI与3-SPI的相关性好于AVI与3-SPI的相关性;(3)运用AWI反演的华北地区2009年夏季干旱分布图较好地反映了旱情的时空分布,与相关气象资料结果相符合。     

基于智慧交通的可达性与交通出行碳排放——理论与实证

信息与通信技术的发展推动了智慧交通建设,并将进一步提高城市交通可达性和影响居民出行。在全球应对气候变化、减少碳排放的共识背景下,由于"智慧交通—可达性—出行行为(碳排放)"间复杂的影响作用机制,已有的研究难以判断智慧化背景下的城市交通可达性提高将增加抑或减少出行碳排放。如何通过提高城市交通可达性来缓解交通拥堵,保障城市交通运输系统的有效运行,并提高居民出行效率,减少出行碳排放是当前中国智慧交通发展面临的关键问题之一。针对以上科学问题,本文尝试提出基于智慧交通的可达性与交通出行碳排放的理论框架,并以广州为研究案例地,研究了社区居民通勤碳排放特征及其影响机理,社区出行低碳指数格局及其影响因素的空间异质性,以及基于碳排放—位置分配模型的公共中心规划支持系统设计与应用,可为今后的相关研究提供借鉴。     

Communities as the agents of commodification: The Kumbo Water Authority in Northwest Cameroon

In 1991 the people of the town of Kumbo in Northwest Cameroon expelled the national water corporation from town by force. They claimed to be retrieving a network that the community rightfully owned and they began to run the system along community lines. The paper tells the story of that water supply twice. First it reproduces the popular accounts that circulated in the 1990s that emphasize the role played by the community in the development and operation of the water supply and portray recent events as a triumph of community management. Then it retells the story using archive evidence that puts a quite different light on some of the events. It is argued that the emphasis on community management has a tendency to obscure other important aspects of the story, for example its political context and consequences. It is suggested that the widespread admiration for any initiative that is community based often results in an uncritical analysis of this form of development project. In this case the commodification of water accelerates after the community has wrested control of this public service from the state.     

GeoSOT-3D椭球体剖分真三维数据表达

随着地球观测技术和信息技术的进步,以全球为背景的科学探索不断增多,不仅是地球表面信息,地下、水下、空中等地球上各个层次的空间信息都受到极大的关注。地球球体剖分网格可以构建真三维的数据模型,进而实现空天地、地下水下一体化的空间数据组织、管理、表达与应用。本文详细论述了GeoSOT-3D椭球体剖分网格的剖分空间框架、剖分编码与剖分尺度特性等理论体系。建立基于GeoSOT-3D的空间对象的真三维表达模型。利用该真三维模型实现了地球椭球体的地上地下可视化,点线面体等空间实体的可视化。研究表明,GeoSOT-3D椭球体剖分具有椭球剖分框架简单、体系完整、二三维一致等特点,因而在该框架下的真三维数据模型可用于下一代地理信息系统中的空间大数据的组织管理与表达。     

小良试验站三种植被类型地表径流效应的对比研究

本文分析研究了３种植被类型下的地表径流在汇流、洪峰及径流过程上的特征，差异是巨大的。在径流过程上桉树林和裸地的变地剧烈，几乎没有什么调蓄作用，洪峰流量占一次性降水径流量的绝大部分，涨水和退水流量都较小。本文应用灰色系统ＧＭ（２，１）模型，成功地预测了以年和以月为时间尺度的地表径流序列，其预测模型可以用来对这些时间尺度的地表径流进行预报。     

显性语法知识传授中的发现原则

尽管语法在交际教学法鼎盛时期一度遭到冷遇，但现在又重新被英语语言教学所接纳，作为一种新的语法教学方式，发现原则综合了传统归纳发和演绎法的长处，概述了发现原则的理论依据，指出发现原则旨在鼓励学习者积极参与而非被动接受，符合当前认知主义对二语学习的认识。     

Potential implications of differential preservation of testate amoeba shells for paleoenvironmental reconstruction in peatlands

Testate amoebae are now commonly used in paleoenvironmental studies but little is known of their taphonomy. There is some experimental evidence for differential preservation of some testate amoeba shell types over others, but it is unclear what, if any impact this has on palaeoenvironmental reconstruction. To investigate this issue we looked at palaeoecological evidence for the preservation of different shell types. We then investigated the possible impact of selective preservation on quantitative palaeoenvironmental inference. We first used existing palaeoecological data sets to assess the vertical patterns of relative abundance in four testate amoeba shell types: (1) shells made of secreted biosilica plates (idiosomes, e.g. Euglypha), (2) idiosomes with thick organic coating (Assulina), (3) proteinaceous shells (e.g. Hyalosphenia), (4) shells built from recycled organic or mineral particles (xenosomes) (e.g. Difflugia, Centropyxis). In three diagrams a clear pattern of decay was only observed for the idiosome type. In order to assess the implications of differential preservation of testate amoeba taxa for paleoenvironmental reconstruction we then carried out simulations using three existing transfer functions and a wide range of scenarios, downweighting different test categories to represent the impact of selective test decomposition. Simulation results showed that downweighting generally reduced overall model performance. However downweighting a shell type only produced a consistent directional bias in inferred water table depth where that shell type is both dominant and shows a clear preference along the ecological gradient. Applying a scenario derived from previous experimental work did not lead to significant difference in inferred water table. Our results show that differential shell preservation has little impact on paleohydrological reconstruction from Sphagnum-dominated peatlands. By contrast, for the minerotrophic peatlands data-set loss of idiosome tests leads to consistent underestimation of water table depth. However there are few studies from fens and it is possible that idiosome tests are not always dominant, and/or that differential decomposition is less marked than in Sphagnum peatlands. Further work is clearly needed to assess the potential of testate amoebae for paleoecological studies of minerotrophic peatlands.     

A GIS-based mapping method on distribution of critical Ecological Situation in China

Introduction Human-induced environmental change in China has led to different consequences due to various causes. They conver certain regions and places where their impacts may threaten the long-term or even the short-term sustainability of human-environmental relationships. Designating such areas as "critical environmental situations," Russian Geographers have developed "red data maps" showing their locations[1]. Yet, there are no fully developed conceptualizations of environmental critical…