4种壳色蛤仔TYR基因的表达特性研究

为了解色素控制基因与壳色的关系,本实验用荧光定量聚合酶链式反应(Polymerase Chain Reaction,PCR)技术分析了酪氨酸酶基因(Tyrosinase,TYR)在菲律宾蛤仔(Ruditapes philippinarum)4种壳色蛤仔和7种组织中的表达特性。结果表明,酪氨酸酶基因在鳃、外套膜、闭壳肌、性腺、内脏团、水管和唇瓣中均有表达,其中外套膜中表达量较高,其次为鳃,在闭壳肌中表达量最少。不同的酪氨酸酶基因在4种壳色的表达量不同,酪氨酸酶基因TYR2、TYR3、TYR6和TYR9在白斑马蛤中表达量居高,且与斑马蛤和白蛤差异显著(P0.05)。TYR2、TYR6和TYR10在橙蛤中表达量最高,推测与橙色形成有关。TYR11在斑马蛤中表达量最高,且与白蛤和白斑马蛤差异显著(P0.05),推测与背景色形成有关。系统发育分析结果表明,TYR3与马氏珠母贝(Pinctada martensii)同源性最高为64%。TYR10与加州双斑蛸(Octopus bimaculoides)同源性最高,为53%。与长牡蛎进化关系最近。     

CMIP5模式对21世纪全球和中国年平均地表气温变化和2℃升温阈值的预估

基于参加国际耦合模式比较计划第5阶段（CMIP5）的29个全球气候模式开展的历史气候模拟和3种典型浓度路径（RCP2.6、RCP4.5、 RCP8.5）下21世纪气候预估的结果,分析了单个模式和多模式集合平均（MME）的21世纪全球与中国年平均地表气温（ASAT）变化特征及2℃升温阈值的出现时间。多模式集合平均的结果显示：全球和中国年平均地表气温均将继续升高,21世纪末的升温幅度随着辐射强迫的增大而增大。RCP2.6情景下,年平均地表气温增幅先升高后降低,全球（中国）年平均地表气温在2056年（2049年）达到升温峰值,21世纪末升温1.74℃（2.12℃）;RCP4.5情景下,年平均地表气温在21世纪前半叶逐渐升高,之后升温趋势减缓,21世纪后期趋于平稳,21世纪末全球（中国）年平均地表气温增幅为2.60℃（3.39℃）;RCP8.5情景下,21世纪年平均地表气温快速升高,21世纪末全球（中国）年平均地表气温增幅为4.75℃（6.55℃）。全球平均的年平均地表气温增幅,在RCP2.6情景下没有超过2℃,RCP4.5和RCP8.5情景下分别在2047和2038年达到2℃。RCP2.6、RCP4.5和RCP8.5情景下中国年平均地表气温增幅连续5 a不低于2℃的时间分别在2032、2033和2027年,明显早于全球平均。任一典型浓度路径情景下,达到2℃升温的时间,北半球同纬度地区早于南半球,同半球高纬度地区早于低纬度地区,同纬度地区陆地早于海洋。3种不同典型浓度路径情景下21世纪全球和中国年平均地表气温将继续升高这一结果是可信的,RCP4.5和RCP8.5情景下全球和中国年平均地表气温增幅超过2℃的结果模式之间有较高的一致性。多模式预估的全球和中国年平均地表气温升幅和不同幅度升温的出现时间均存在一定的不确定性,预估结果的不确定性随预估时间的延长而增大;相同情景下,中国年平均地表气温预估的不确定性大于全球。     

A numerical comparison of time and frequency‐domain marine electromagnetic methods for hydrocarbon exploration in shallow water

In shallow water the frequency domain controlled source electromagnetic method is subject to airwave saturation that strongly limits the sensitivity to resistive hydrocarbon targets at depth. It has been suggested that time‐domain CSEM may offer an improved sensitivity and resolution of these deep targets in the presence of the airwave. In order to examine and test these claims, this work presents a side‐by‐side investigation of both methods with a main focus on practical considerations, and how these effect the resolution of a hydrocarbon reservoir. Synthetic noisy data for both time‐domain and frequency domain methods are simulated using a realistic frequency dependent noise model and frequency dependent scaling for representative source waveforms. The synthetic data studied here include the frequency domain response from a compact broadband waveform, the time‐domain step‐response from a low‐frequency square wave and the time‐domain impulse response obtained from pseudo‐random binary sequences. These data are used in a systematic resolution study of each method as a function of water‐depth, relative noise and stacking length. The results indicate that the broadband frequency domain data have the best resolution for a given stacking time, whereas the time‐domain data require prohibitively longer stacking times to achieve similar resolution.     

Risk-based assessment of arsenic-affected aquacultural water in blackfoot disease hyperendemic areas

This work explored a risk-based arsenic (As) regulation in farmed pond water by ingesting tilapia (Oreochromis mossambicus) in blackfoot disease hyperendemic areas and discussed a rational As regulation in pond water. Monte Carlo analysis was used to propagate the parameter uncertainty and to assess probabilistically regulation risks. A dynamic scheme of groundwater management was proposed that curves of utilization ratios against As concentrations in groundwater were established based on the risk-based regulation. The 5th to 95th percentiles of risks range from 3.5 × 10⁻⁷ to 6.0 × 10⁻⁵ via ingesting the farmed tilapia under the current As regulation in farmed pond water in Taiwan, 50 μg/L. To compare to inorganic As regulation in drinking water, the current As regulation in farmed pond water does not pose a great threat to human health, but it is unsafe. Therefore, this study suggests that the regulation of As in farmed pond water is revised to be 25 μg/L.     

中国南方寒武纪岩相古地理

中国南方是指西至金沙江-元江断裂、西北至龙门山断裂、北至城口-房县-襄樊-广济断裂、东北至郯庐断裂、东至黄海和东海、南至南海的我国南方广大地区。在各露头剖面和钻井剖面地层学和岩石学研究所取得的各种定量及定性资料的基础上,采用单因素分析综合作用法,编制出了中国南方下寒武统下部和中部、下寒武统上部、中寒武统和上寒武统的各种单因素图以及相应的早寒武世早期和中期、早寒武世晚期、中寒武世和晚寒武世岩相古地理图。这些古地理图的最主要特征是定量,即每个古地理单元的划分和确定都有确切的定量的单因素图为依据。这种定量的岩相古地理图在中国南方寒武纪还是首次出现。中国南方寒武纪有7个主要的古地理单元,即滇西台地、康滇陆、扬子台地、斜坡、江南盆地、东南台地和华夏陆。滇西台地、康滇陆、扬子台地、斜坡和江南盆地属康滇古地理体系,东南台地和华夏陆属华夏古地理体系。这两个古地理体系拼合在一起,就构成了中国南方寒武纪的两陆、三台、一盆和一坡和古地理的基本格局。是寒武世早期和中期的岩相古地理特征与早寒武世晚期、中寒武世和晚寒武世岩相古地理特征有明显的不同。这是两个大不相同的演化阶段。这种定量的岩相古地理图在古地理学中是个重大的进展,对石油、天然气以及其他各种矿产的预测和勘探有重要的指导作用。     

Generating probabilistic estimates of hydrological response for Irish catchments using a weather generator and probabilistic climate change scenarios

In accounting for uncertainties in future simulations of hydrological response of a catchment, two approaches have come to the fore: deterministic scenario‐based approaches and stochastic probabilistic approaches. As scenario‐based approaches result in a wide range of outcomes, the role of probabilistic‐based estimates of climate change impacts for policy formulation has been increasingly advocated by researchers and policy makers. This study evaluates the impact of climate change on seasonal river flows by propagating daily climate time series, derived from probabilistic‐based climate scenarios using a weather generator (WGEN), through a set of conceptual hydrological models. Probabilistic scenarios are generated using two different techniques. The first technique used probabilistic climate scenarios developed from statistically downscaled scenarios for Ireland, hereafter called SDprob. The second technique used output from 17 global climate models (GCMs), all of which participated in CMIP3, to generate change factors (hereafter called CF). Outputs from both the SDprob and the CF approach were then used in combination with WGEN to generate daily climate scenarios for use in the hydrological models. The range of simulated flow derived with the CF method is in general larger than those estimated with the SDprob method in winter and vice versa because of the strong seasonality in the precipitation signal for the 17 GCMs. Despite this, the simulated probability density function of seasonal mean streamflow estimated with both methods is similar. This indicates the usefulness of the SDprob or probabilistic approach derived from regional scenarios compared with the CF method that relies on sampling a diversity of response from the GCMs. Irrespective of technique used, the probability density functions of seasonal mean flow produced for four selected basins is wide indicating considerable modelling uncertainties. Such a finding has important implications for developing adaptation strategies at the catchment level in Ireland. Copyright © 2011 John Wiley & Sons, Ltd.     

A Monte-Carlo game theoretic approach for Multi-Criteria Decision Making under uncertainty

Game theory provides a useful framework for studying Multi-Criteria Decision Making problems. This paper suggests modeling Multi-Criteria Decision Making problems as strategic games and solving them using non-cooperative game theory concepts. The suggested method can be used to prescribe non-dominated solutions and also can be used as a method to predict the outcome of a decision making problem. Non-cooperative stability definitions for solving the games allow consideration of non-cooperative behaviors, often neglected by other methods which assume perfect cooperation among decision makers. To deal with the uncertainty in input variables a Monte-Carlo Game Theory (MCGT) approach is suggested which maps the stochastic problem into many deterministic strategic games. The games are solved using non-cooperative stability definitions and the results include possible effects of uncertainty in input variables on outcomes. The method can handle multi-criteria multi-decision-maker problems with uncertainty. The suggested method does not require criteria weighting, developing a compound decision objective, and accurate quantitative (cardinal) information as it simplifies the decision analysis by solving problems based on qualitative (ordinal) information, reducing the computational burden substantially. The MCGT method is applied to analyze California’s Sacramento-San Joaquin Delta problem. The suggested method provides insights, identifies non-dominated alternatives, and predicts likely decision outcomes.     

短时交通流量模式提取及时变特征分析

提出了一种基于三步策略的方法来提取交通流模式并对其时变特征进行分析。首先,根据交通流量内部变化将其分割成非等长的一系列具有明显物理意义的子序列;然后,利用定量递归分析（recurrence quantification analysis,RQA）提取各子序列的统计参数;最后,通过聚类获得交通流典型模式。实验结果表明,此方法能有效提取交通流中隐含的4种模式及它们在全天中的时间分布和时变特征,以及它们在工作日和非工作日时态分布上的差异。     

Uncertain climate: An investigation into public scepticism about anthropogenic climate change

This study presents a detailed investigation of public scepticism about climate change in Britain using the trend, attribution, and impact scepticism framework of Rahmstorf (2004). The study found that climate scepticism is currently not widespread in Britain. Although uncertainty and scepticism about the potential impacts of climate change were fairly common, both trend and attribution scepticism were far less prevalent. It further showed that the different types of scepticism are strongly interrelated. Although this may suggest that the general public does not clearly distinguish between the different aspects of the climate debate, there is a clear gradation in prevalence along the Rahmstorf typology. Climate scepticism appeared particularly common among older individuals from lower socio-economic backgrounds who are politically conservative and hold traditional values; while it is less common among younger individuals from higher socio-economic backgrounds who hold self-transcendence and environmental values. The finding that climate scepticism is rooted in people's core values and worldviews may imply a coherent and encompassing sceptical outlook on climate change. However, attitudinal certainty appeared mainly concentrated in non-sceptical groups, suggesting that climate sceptical views are not held very firmly. Implications of the findings for climate change communication and engagement are discussed.