Tracing anthropogenic inputs to production in the Seto Inland Sea, Japan - A stable isotope approach

The Seto Inland Sea (SIS) receives waste runoff from ∼24% of Japan’s total population, yet it is also important in regional fisheries, recreation and commerce. During August 2006 we measured carbon and nitrogen stable isotopes of particulate organic matter (POM) and zooplankton across urban population gradients of the SIS. Results showed a consistent trend of increasing δ¹⁵N in POM and zooplankton from the western to eastern subsystems of the SIS, corresponding to increasing population load. Principal components analysis of environmental variables indicated high positive loadings of δ¹⁵N and δ¹³C with high chlorophyll-a and surface water temperatures, and negative loadings of low salinities related to inputs from large rivers and high urban development in the eastern SIS. Anthropogenic nitrogen was therefore readily integrated into the SIS food web from primary production to copepods, which are a critical food source for many commercially important fishes.     

Trophic analysis of two species of Atopsyche (Trichoptera: Hydrobiosidae)

To compare the trophic niche of coexisting larvae of Atopsyche yunguensis and Atopsyche spinosa we analysed their gut contents and found little niche overlap: A. yunguensis fed primarily on Trichoptera and amorphous matter, whereas A. spinosa fed on Diptera. A. spinosa showed both a spatial range and a niche breadth larger than those of A. yunguensis. Behavioural observations on A. spinosa may emphasize the use of chelate anterior legs for securing preys. Both species have been included into the predator-clingers functional group.     

Holocene trophic state changes in relation to sea level variation in Lake Blanca, SE Uruguay

Paleolimnological data are presented relating trophic development to sea level variation in Lake Blanca, a small (0.6 km²), coastal fresh waterbody in southern Uruguay. Using a sediment core that extended to 7300 years BP, analyses of grain size, thin sections, organic matter, carbonate, total carbon, nutrients, diatoms and palynomorphs, allowed us to infer changes in trophic state and paleosalinities, which were closely related to Holocene sea level variation. Higher trophic states were observed during regressive events, most probably due to increases in runoff and erosion as regression progressed. Four diatom association zones (DAZ) were identified in the sediment core. The basal core section pre-dated the first Holocene marine transgression, contained no diatoms, chrysophyte cysts or non-siliceous microalgae, and showed C/N ratios values higher than 20. Thus, it is likely that the system exhibited terrestrial characteristics. In the second section (6500–2200 years BP, following the first Holocene transgression), there was dominance of marine/brackish diatom species. The lowest trophic states of the core were observed in this section. The third section (2200–1100 years BP), represented the system as it became separate from the Atlantic Ocean, and showed a dominance of brackish/freshwater species and increases in trophic state were observed. In the last section (after 1100 years BP), the system became fully freshwater as no marine or brackish diatom species were found, but a trend to oligotrophication was observed, probably due to nutrient depletion. However, after 1967 AD, eutrophication intensified because of forestry and soil fertilization in the catchment. Pollen association zones (PAZ) allowed us to identify four sections. Below 250 cm (2200 years BP), the core contained no pollen grains as redox potential and pH values were not conducive for pollen preservation. After 2200 years BP (when the system started to separate from the ocean), xerophilic taxa typical of coastal dunes colonized the catchment. Only after 1100 years BP (after fully freshwater conditions established) pollen grains of trees were observed.     

Relationships between Trophic State, Paleosalinity and Climatic Changes during the First Holocene Marine Transgression in Rocha Lagoon, Southern Uruguay

Paleolimnological data are presented on trophic development, climatic change and sea level variations in Rocha Lagoon, a 72 km² coastal lagoon in southern Uruguay. Using a sediment core that extended from 7000 to about 3700 yr BP, analyses of organic matter, carbonate, diatoms and chrysophyte cysts were used to track the early Holocene paleolimnological conditions of Rocha Lagoon. Opal phytoliths were also counted and identified, both temperature and humidity indices were calculated, and Opal Phytolith Association Zones (OPAZ) were identified by performing Principal Coordinates Analysis (PCO). Diatom Association Zones (DAZ) corresponding to marine/brackish and brackish/freshwater episodes were closely related to changes in trophic state. Those DAZ representing marine/brackish stages exhibited a lower trophic state than those DAZ dominated by brackish and freshwater diatoms. This highlights that during the first Holocene marine transgression, Rocha Lagoon did not continuously exhibit marine/brackish conditions as reported in previous papers. Instead, three brackish/freshwater episodes related to sea level variation and changes in humidity were identified. The first episode, by ~6000 yr BP, was related to sea level change as no significant changes in either temperature or humidity indices were observed. The second episode, between 5000 and 4400 yr BP, was related to both a sea level decrease and an increase in humidity, as a transition from humid to very humid climate was inferred. Concomitant decreases in salinity and increases in trophic state were also observed. The third episode, after ~4000 yr BP, was related to the end of the first Holocene regressive phase when sea level was slightly below present levels. Further decreases in salinity and increases in trophic state were detected. The paleoclimatic trends inferred in this study were in close agreement with other regional studies on climatic change, as cool temperatures were inferred. However, major changes in humidity were also detected. A humid to very humid climate was inferred for ~7000–4500 yr BP, but the occurrence of a semiarid/arid climate was inferred for ~4500–3700 yr BP. Our data suggest that during transgressive and regressive events there might be higher frequency and lower amplitude sea level oscillations that might lead to changes in salinity and trophic state of coastal aquatic systems. Such oscillations could only be tracked by high resolution analyses of sedimentary records and could be best interpreted with complementary data on paleoclimate. In addition, microfossils such as diatoms and opal phytoliths were shown to be very sensitive to such paleoenvironmental changes.     

Chemical composition and the nitrogen-regulated trophic state of Patagonian lakes

Chemical composition and nutrient concentrations of 39 relatively poorly known Patagonian lakes (38–50°S and from 70° to 68°W) are described and analysed using principal component analysis (PCA). The general relationships between nutrients (total phosphorus, bioavailable phosphorus and dissolved inorganic nitrogen) and plankton biomass are examined.We seek to demonstrate that the extreme oligotrophy characterising many lakes and reservoirs of the Argentine Patagonian region of South America owes more to nitrogen deficiency than to a shortage of available phosphorus. The data show a range of trophic conditions with variable water chemistry characteristics.The first two axes of the PCA ordination explain most of the variance (63%). The first component of the variance in the environmental data is a trophic gradient, with positive correlations with the concentrations of nutrients (TP, SRP, DIN) and electrical conductivity and a negative correlation with transparency. For all the reservoirs considered, the calculated annual, summer and winter chlorophyll-a carrying capacities of the available phosphorus were consistently and significantly (P<0.05) in excess of observations but maximum chlorophyll-a values correlate with DIN availability. Indeed the chlorophyll-a carrying capacities, as an index of the resource-sustainable maximum biomass, of the available nitrogen gives a better predictive yield relationship than does P. Our findings are remarkable in so far as the general expectation that dinitrogen-fixing phytoplankton should thrive in the absence of dissolved inorganic nitrogen, at least to the supportive limits of the available phosphorus, is unfulfilled.     

Spatial variability in the trophic ecology and biology of the deep-sea shrimp Aristaeomorpha foliacea in the Mediterranean Sea

The trophic ecology, energy and reproductive states of the deep-water shrimp Aristaeomorpha foliacea, widely distributed along the slopes of the Mediterranean Sea Basins, were analysed in eight areas spread along ca. 3000 km in order to identify patterns in the habitat conditions supporting the species. From W to E the areas were situated between the north side of Eivissa (39°12′N, 1°20′E, in the Balearic Basin) and off Mersin, Turkey (36°15′N, 34°19′E, in the Levantine Sea). Trends identified mainly as a function of longitude from west to east were: (i) higher δ¹⁵N, parallel to δ¹⁵N shifts in the top 200 m of the water column for particulate organic N (Pantoja et al., 2002). The δ¹⁵N trend indicates that the deep trophic web, i.e. A. foliacea at 400–600 m, reflects the δ¹⁵N signal of the photic zone; (ii) a similar significant trend of δ¹³C, related with exploitation of pelagic versus benthic resources by A. foliacea in each area (i.e. by local variability of terrigenous inputs via submarine canyons). More depleted δ¹³C was found at mid-longitudes (Tyrrhenian Sea and Sicily Channel) linked to higher consumption of macroplankton prey (Pasiphaea spp., euphausiids and mesopelagic fishes). The feeding intensity (gut fullness, F) and prey diversity (J) of A. foliacea were related, according to generalized linear models, with the temperature and salinity of intermediate waters, variables in turn associated with latitude and longitude. Both F and J were higher in areas with greater shrimp density. The optimal ecological habitat of A. foliacea appears to be located in the Tyrrhenian Sea and the Sicily Channel, where we found the highest F, the greatest trophic diversity and A. foliacea in the best biological condition (i.e. with higher hepato-somatic index, HSI). These are also the areas with the highest densities of A. foliacea. In contrast, in the western Mediterranean Sea (Balearic Basin and the southern Balearic Islands), where A. foliacea has low densities, the shrimp showed generally lower values of trophic indicators and biological condition.