半滑舌鳎稚鱼脂肪分解关键酶基因的克隆及饲料中脂肪水平对其表达的调控

克隆了半滑舌鳎脂肪分解关键酶基因即激素敏感脂肪酶(Hormone-sensitive lipase,HSL)和脂肪甘油三酯脂肪酶(Adipose triglyceride lipase,ATGL)cDNA部分序列,并探讨了饲料中脂肪水平对半滑舌鳎稚鱼HSL及ATGL基因表达的影响。克隆所得到的HSL和ATGL片段长度分别为589bp和581bp,序列和系统进化分析表明半滑舌鳎的HSL和ATGL与大部分鱼类具有较高的同源性。采用不同水平的鱼油配制成5种不同脂肪水平(6.68%、9.84%、13.47%、17.89%和21.88%干物质)的等氮饲料,饲养35日龄半滑舌鳎稚鱼30d,养殖过程中每天饱食投喂5次,养殖实验结束后采用实时荧光定量PCR分别测定各处理稚鱼内脏团的HSL及ATGL mRNA相对表达量。结果表明,高脂饲料组(21.88%)显著促进了半滑舌鳎稚鱼内脏团HSL基因的表达(P0.05),但饲料脂肪水平对于半滑舌鳎稚鱼内脏团的ATGL表达量未产生显著影响(P0.05)。     

Initiation and evolution of intra-oceanic subduction in the Uralides: Geochemical and isotopic constraints from Devonian oceanic rocks of the Southern Urals, Russia

Abstract The Southern Uralides are a collisional orogen generated in the Late Devonian–Early Carboniferous by the collision of the Magnitogorsk island arc (MA) generated in the Early to Middle Devonian by intra‐oceanic convergence opposite to the continental margin, and the continental margin of the East European craton. A suture zone of the arc to the continental margin, the Main Uralian Fault (MUF), is marked by ophiolites and exhumed high‐pressure–low‐temperature metamorphic rocks of continental origin. The pre‐orogenic events of the Southern Urals and their geodynamic setting are traced by means of fluid‐immobile incompatible trace elements (rare earth elements and high field strength elements) and Sr–Nd–Pb isotope geochemistry of the MA suites, in particular the protoarc suite with boninites and probably ankaramites, and the mature arc comprised of island arc tholeiitic (IAT) suites, transitional IAT to calc‐alkaline (CA), and CA suites. The MA volcanics result in genetically distinct magmatic source components. In particular, depleted normal‐mid‐oceanic ridge basalt‐type mantle sources with various enrichments in a slab‐derived aqueous fluid component are evident. The enriched component is not involved in significant amounts, as testified by the rather radiogenic Nd isotopes and unradiogenic Pb isotopes. Further information on the pre‐orogenic events is provided by the Mindyak Massif metagabbros derived from diverse gabbroic protoliths that were affected by oceanic rodingitization, and subsequently by a high‐temperature (HT) metamorphism related to the development of a metamorphic sole. The HT metamorphism has the same age as the protoarc volcanism, and constrains the initiation of subduction at approximately 410 Ma. Consequently, the maximum timespan between initial intra‐oceanic convergence and final collision is approximately 31 my, a duration consistent with that of present‐day ongoing collisions in the western Pacific. The characteristics of early volcanism and the traces of a metamorphic sole provide useful criteria to attribute most MUF ophiolites to the Tethyan type with a complex pre‐orogenic evolution.     

Anatomy of a transgressive lag: Panther Tongue Sandstone, Star Point Formation, central Utah

Abstract The Panther Tongue of the Star Point Formation in central Utah contains a variety of transgressive lag deposits that, when mapped regionally, show a sensitive dependence upon pre‐existing topography of the palaeoshoreline. The Panther Tongue consists of a coarsening‐upward sandstone wedge that prograded into the Western Interior Seaway during Late Cretaceous (Santonian) time. High‐resolution sequence stratigraphic analysis revealed that this member was deposited during the long‐distance (>50 km) regression and transgression of a delta into shallow‐marine environments, containing basal highstand, forced regression, lowstand and transgressive systems tracts. Based on grain size, clast composition, lateral extent and stratigraphic position, the coarse sandstones on top of the Panther Tongue were classified into four types: (1) simple; (2) dispersed; (3) oxidized; and (4) local lags. The simple lag is composed of dark grey coarse sandstone with oyster fragments and shark teeth. This lag is typically extensively bioturbated and massive. Laminated and cross‐bedded units are also common. This type of coarse sandstone is interpreted as a typical transgressive lag. The dispersed lag differs in that it contains abundant mud and commonly occurs as multiple beds in thick intervals of muddy sandstone. Mixing of bay/estuarine and shallow ‐ marine mud with simple lag sand may be responsible for deposition of this type of coarse sandstone. The oxidized lag is distinctive in its reddish colour with extensive bioturbation and is commonly overlain by a simple lag. The local lag is composed of thin‐bedded, dark grey, coarse sandstone, occurring locally between the mouth bar and distributary channel. The variation in types, grain size and bed thickness of the coarse‐grained lags was mainly controlled by antecedent topography as suggested by immediately underlying lithofacies. Relatively thick (≈30 cm) simple lags are present on top of mouth‐bar sandstones, whereas dispersed lags are common on top of the distributary channel sandstone and in bay/estuarine and shallow‐marine mudstones. Erosion of topographic highs (mouth bar) resulted in relatively thick accumulation of simple lags. In topographic low areas such as distributary channel, estuary, bay and shallow‐marine environments, fine‐grained muddy sands that were eroded from the nearby topographic highs were redeposited. Intermittent storm waves transported coarse sands both landward and seaward, forming a dispersed lag. The net effect was reworking of local topographic relief during overall transgression, forming an apparently planar transgressive surface of erosion.     

Counterclockwise P-T-t trajectory from the metamorphic sole of a Neo-Tethyan ophiolite (Turkey)

The Kiziltepe ophiolitic thrust sheet in the Bolkar Mountains of Turkey occurs between two subparallel ophiolite belts bounding the Tauride carbonate platform and represents a remnant of the Cretaceous Neo-Tethyan oceanic lithosphere. It is underlain by foliated amphibolite that represents a metamorphic sole developed at the inception of an intra-oceanic subduction zone in the Neo-Tethys 92-90 Ma. Blueschist-facies overprinting of the amphibolite indicates that the metamorphic sole was dragged deeper into the subduction zone where it experienced increasing P/T with cooling. Regional tectonic constraints suggest a Maastrichtian age for the timing of this blueschist-facies metamorphism. Sodic amphibole-rich veins and crossite/Mg-riebeckite rims on hornblende suggest that growth of blueschist-facies minerals was facilitated by infiltration of fluid along fractures and grain boundaries. We infer a counterclockwise P-T-t trajectory during which metamorphism was accompanied/succeeded by rapid uplift along the northern edge of the Tauride belt in Late Cretaceous-early Tertiary time.     

Impact of climate and hydrodynamics on sole larval immigration towards the Tagus estuary, Portugal

Spawning grounds of the soles, Solea solea and Solea senegalensis, are distant from the estuarine nurseries where juveniles concentrate. Recruitment of these species is highly dependent on the success of the larval migration towards the inshore nursery grounds. Unfavourable climate and hydrodynamic circulation may lead to high mortality rates at this stage. The relation between river drainage, NAO index and the North-South wind component intensity over the three months prior to the end of the estuarine colonization and the densities of S. solea and S. senegalensis in the nursery grounds were investigated for both species based on a discontinuous historical dataset (from 1988 to 2006) for the Tagus estuary. Multiple linear regression models were developed for sole density and environmental data (separately for each species). Results showed that river drainage is positively correlated with juveniles’ densities of both species, possibly due to the existence of chemical cues used by larvae for movement orientation. NAO index and the North-South wind component intensity relations with soles densities were non-significant. It was concluded that the high complexity of the Portuguese upwelling system makes it hard to detect causal relations of the environmental variables tested. The importance of river flow for coastal ecosystems was stressed. Since climate change scenarios predict a strong decrease in rain fall over the Portuguese river basins, as well as a concentrated period of heavy rain in winter, it was hypothesised that future river drainage decrease over much of the year may lead to lower recruitment success for soles, especially for S. senegalensis.     

半滑舌鳎(Cynoglossus semilaevis)与摄食行为相关的特定感觉器官研究

在实验室条件下,通过特定感官消除或抑制方法和特定感官刺激方法对半滑舌鳎摄食行为反应机制进行了研究。结果表明,半滑舌鳎主要依靠侧线摄食,其侧线主要对猎物的低频振动起反应;嗅觉起辅助作用,头部各边缘部位和躯干中上部各鳍具有部分作用;视觉在捕食中的作用不大,味觉在食物吞咽过程中起很大作用。由于半滑舌鳎主要利用侧线捕食猎物,嗅觉起辅助作用,在人工养殖条件下,应根据其摄食生理特点,设计投喂饲料,制定投喂策略。     

Age of the metamorphic sole of the Papuan Ultramafic Belt ophiolite, Papua New Guinea

The Papuan Ultramafic Belt (PUB) ophiolite is former oceanic crust and upper mantle emplaced onto continental crust in Papua New Guinea (PNG) in a zone of general convergence between the Pacific and Australian plates. The metamorphic sole beneath the ophiolite is best exposed in the Musa–Kumusi divide and comprises a 40- to 300-m-thick body of granulite and amphibolite facies rocks. Geochronological studies on the metamorphic sole, using amphiboles from the granulites and amphibolites, yield measured K–Ar ages ranging from 65.0±0.7 to 57.2±0.6 Ma and average ⁴⁰Ar–³⁹Ar direct total fusion ages ranging from 67.0±0.7 to 59.5±0.2 Ma. Five of the six ⁴⁰Ar–³⁹Ar plateau ages, derived from age spectra, lie between 58.6±0.2 and 57.8±0.2 Ma, with an overall mean age of 58.3±0.4 Ma. The large spread in measured K–Ar and ⁴⁰Ar–³⁹Ar total fusion ages is thought to be caused by the presence of variable amounts of excess argon. The mean plateau age for five samples of 58.3±0.4 Ma is interpreted to mark the time of cooling of the metamorphic sole following peak metamorphism. We suggest that the development of the metamorphic sole and emplacement of the PUB ophiolite onto the PNG crust occurred in a relatively short time interval in the Paleocene.     

人工养殖半滑舌鳎卵巢发育及其产卵类型研究

研究了人工养殖半滑舌鳎(Cynoglossus semilaevis)卵巢发育组织学的季节变化及其与性成熟系数(gonadosomatic index,简称GSI)和肝质量指数(hepatosomatic index,简称HSI)的关系。结果表明,半滑舌鳎成熟鱼卵巢发育经历Ⅱ期、Ⅲ期、Ⅳ期、Ⅴ期、Ⅵ期,再到重复发育Ⅱ期;人工养殖条件下,3~5月份大部分鱼卵巢处于Ⅱ期,从6月份开始,其卵母细胞内开始沉积卵黄颗粒,9~11月份,卵母细胞内卵黄颗粒充盈整个胞质,基本达到生长成熟,排卵后至翌年2月份,卵巢进入了退化吸收Ⅵ期或重复发育Ⅱ期;繁殖期GSI达到最高峰;产卵后或重复发育Ⅱ期,GSI显著下降,此时HSI显著升高至全年最高峰;半滑舌鳎在1 a中只有1个繁殖期,进行分批产卵。