山东日照和福建厦门沿海花鲈(Lateolabrax japonicus)遗传多样性的RAPD研究

采用RAPD技术 ,分析了日照、厦门沿海花鲈两群体的遗传多样性水平。结果表明 ,31条随机引物共扩增出两地花鲈 45 5条RAPD标记带 ,其中日照群体占 2 63条 ,厦门群体为1 92条 ;45 5条中有 1 84条带为两群体共有。日照群体内遗传相似度I为 0 892 8(差异度为0 1 0 72 ) ,厦门群体I为 0 92 2 5 (差异度为 0 0 775 ) ,而两者群体间的遗传相似度I为 0 8338(遗传距离D =0 1 662 )。两地花鲈在基因组DNA水平上存在较大的遗传差异。利用随机引物S1 1、S42、S45、S5 2和S2 0 3,在花鲈两群体间扩增出 8条稳定、明显的群体间特异性标记带     

无公害刺参稳产健康养殖新技术

本文针对目前刺参养殖中存在的问题,简单明了叙述刺参无公害健康养殖一系列新技术和新方法,对推动海参养殖业持续、稳定、健康发展,起到积极作用和效果。     

温度变化对刺参同工酶的影响

采用同工酶电泳方法,对在不同温度处理下刺参(Apostichopus japonicus)的乳酸脱氢酶(LDH)、酯酶(EST)和超氧化歧化酶(SOD)的表达情况进行分析。本文结果显示刺参组织内无LDH同工酶的表达。在10℃到20℃的温度突变处理中,12 h内EST和SOD均有新增酶带,在20℃到10℃的温度突变1 h内EST表达有新增酶带。3 h内SOD表达有新增酶带。在不同变温幅度的处理中,EST和SOD只有1条酶带。这表明,刺参对温度突变可产生积极的响应,对于其适应环境温度的变化具有重要意义。     

不同温度的有机溶剂分提对鯷鱼鱼油得率及脂肪酸组成的影响

讨论了不同温度的有机溶剂分级提取对鳀鱼鱼油得率、脂肪酸组成及EPA、DHA含量变化的影响。经15℃干法分级提取的液态鳀鱼鱼油,在设定的4种温度下,液态鳀鱼鱼油得率均随溶剂量的增加而提高;在相同溶剂量的情况下,随着分级提取温度的降低,液态鳀鱼鱼油得率呈下降的趋势。有机溶剂分级提取对14碳短碳链的饱和与不饱和脂肪酸及16碳、18碳的饱和脂肪酸的脱除效果是明显的,但对脱除20碳、22碳的长碳链饱和脂肪酸的效果不明显。在4种试验温度下,EPA和DHA的总量分别为29.88%、31.74%、35.27%和41.80%,高出美国阿拉斯佳深海鱼油对应总量(28.76%)的1.12%～13.04%。     

四种重金属对刺参幼参的急性致毒效应

采用静水试验法,在水温18.5℃~20℃的条件下,用Cu2 ,Zn2 ,Cd2 和Cr6 四种重金属对刺参幼参进行了单一急性毒性试验。结果表明:Cu2 对刺参幼参的24h,48h,72h和96hLC50分别为0.299,0.176,0.133和0.120mg/L;Zn2 的24h,48h,72h和96hLC50分别为6.700,3.624,2.577和1.951mg/L;Cd2 的24h,48h和72hLC50分别为4.246,2.588和2.137mg/L;Cr6 的24h,48h和72hLC50分别为31.974,7.499和3.808mg/L;Cu2 ,Zn2 ,Cd2 和Cr6 对刺参幼参的安全浓度分别为0.018,0.362,0.259和0.750mg/L。四种重金属对刺参幼参的毒性大小依次为Cu2 >Cd2 >Zn2 >Cr6 。     

南海北部湾秋季蓝圆鲹与竹筴鱼的摄食生态及食物竞争

【目的】研究南海北部湾海域秋季蓝圆鲹与竹筴鱼的摄食生态特征和种间食物竞争。【方法】采用胃含物分析法,对2017年10月北部湾底拖网调查和港口随机取样收集的119尾蓝圆鲹(Decapterusmaruadsi)和198尾竹筴鱼(Trachurus japonicus)胃含物样品进行分析,通过肉眼直接观察或显微镜间接观察胃中残留的饵料,饵料种类鉴定、计数、称重等对食性做定量分析,以估算两种鱼类的营养级和生态位宽度,并利用生态位重叠研究两种鱼类的食物竞争关系。【结果与结论】蓝圆鲹的饵料种类19种(属),以桡足类和小型鱼类为主,优势饵料生物为布氏半棱鳀(Encrasicholinapunctifer);竹筴鱼的饵料种类18种(属),以小型鱼类和浮游甲壳类为主,优势饵料为中国毛虾(Acetes chinensis)。两种鱼类均为游泳动物食性,均存在摄食转换,随着叉长的增长,蓝圆鲹的饵料生物由小型浮游动物为主转变为小型鱼类为主,竹筴鱼的饵料生物由小型鱼类为主转变为以樱虾类和较大鱼类为主。蓝圆鲹和竹筴鱼的空胃率、竹筴鱼的平均胃饱满指数均随个体生长呈显著变化(P<0.05),但蓝圆鲹的平均胃饱满指数随个体生长无显著变化(P>0.05)。蓝圆鲹和竹筴鱼的营养级分别为3.63和3.40,营养生态位宽度分别为1.70和1.24,生态位重叠系数0.56,表明两种鱼类之间存在一定的食物竞争。     

日本鬼鱼由工厂化育苗技术研究

从北部湾沿海采捕的日本鬼鱼由 ( Inimicus japonicus)亲鱼 ,经暂养后进行催产 ,雌鱼每千克体重注射 L RH-A3 1 0μg DOM 5mg或 HCG 60 0～ 80 0 IU LRH-A3 3 0～ 4 0μg;雄鱼注射同样药物 ,剂量减半。共采卵 1 0 9万粒 ,平均受精率 89.0 % ,平均孵化率 90 .0 % ,共培育出体长 1 .8～ 2 .3 cm的幼鱼 2 6.8万尾 ,平均成活率 3 0 .7%。     

Identification and characterization of 43 microsatellite markers derived from expressed sequence tags of the sea cucumber (Apostichopus japonicus)

The sea cucumber Apostichopus japonicus is a commercially and ecologically important species in China. A total of 3056 potential unigenes were generated after assembling 7597 A. japonicus expressed sequence tags (ESTs) downloaded from Gen-Bank. Two hundred and fifty microsatellite-containing ESTs (8.18%) and 299 simple sequence repeats (SSRs) were detected. The average density of SSRs was 1 per 7.403 kb of EST after redundancy elimination. Di-nucleotide repeat motifs appeared to be the most abundant type with a percentage of 69.90%. Of the 126 primer pairs designed, 90 amplified the expected products and 43 showed polymorphism in 30 individuals tested. The number of alleles per locus ranged from 2 to 26 with an average of 7.0 alleles, and the observed and expected heterozygosities varied from 0.067 to 1.000 and from 0.066 to 0.959, respectively. These new EST-derived microsatellite markers would provide sufficient polymorphism for population genetic studies and genome mapping of this sea cucumber species.     

Effect of Bacillus baekryungensis YD13 Supplemented in Diets on Growth Performance and Immune Response of Sea Cucumber（Apostichopus japonicus）

The effect of a potential probiotic on the growth performance and immune response of sea cucumber（Apostichopus japonicus） was investigated. Bacillus baekryungensis YD13 isolated from sea cucumber culturing ponds was added to sea cucumber basal feed as a probiotic in different doses（0, the control; 1×10^4（YD134）, 1×10^6（YD136） and 1×10^8（YD138） CFU g-1 of diet）, and administered orally to A. japonicus（initial mean wet weight 5.44 g ± 0.17 g）. The sea cucumbers were fed in 20 aquaria, 5 each treatment, for 60 d. At the end of growth trial, 20 sea cucumbers from each treatment were challenged with Vibrio splendidus. A. japonicus in YD134 and YD136 exhibited significantly better growth performance than control（P 〈0.05）. Five non-specific immune parameters including lysozyme, acid phosphatase, alkaline phosphatase, superoxide dismutase and catalase in coelomic fluid were measured to evaluate the immune response of A. japonicus to the probiotics. Results showed that all parameters were significantly improved when YD11 was supplemented in the dose of 1×10^6 CFU g^-1（P 〈0.05）. The cumulative incidence and mortality after the Vibrio splendidus challenge decreased significantly in sea cucumbers of YD136. Accordingly, 1×10^6 CFU g^-1 of YD13 in diet was recommended for the growth promotion and immune enhancement of A. japonicus.     

Relationships between body weight and other morphological traits in young sea cucumbers Apostichopus japonicas

Correlation, multiple regression, and path analyses were used to investigate the relationships between body weight and three other morphological traits in juvenile Japanese sea cucumbers Apostichopus japonicus . We measured live body weight (BW), body length (BL), numbers of papillae (NP), and numbers of tube feet (NF) at 60, 80, 100, and 130 days post-hatching (dph). We calculated path correlation coefficients, correlation indices ( R 2 ), and coefficients of determination with BW as the dependent variable and the other morphological traits as independent variables. The coefficient of variation for BW was high across all age groups, and all measured morphological traits were significantly correlated ( P < 0.01). BL had the greatest direct eff ect on BW across all age groups (60 dph, 0.526;80 dph, 0.404;100 dph, 0.620;and 130 dph, 0.681), while NF had the greatest indirect eff ect on BW across all age groups (60 dph, 0.528;80 dph, 0.452;100 dph, 0.666;and 130 dph, 0.603). Regression analyses between morphological traits and BW indicated that R 2 was greater than 0.85 only in the 100-dph specimens. The indirect eff ects of the other measured morphological traits on BW were age-dependent. The optimal regression equations, as determined with stepwise regression, were, for 60-dph specimens: BW 60 =10 (-3.04+0.092BL+0.014NP+0.014NF)( R 2 =0.632);for 80-dph specimens: BW 80 =10 (- 3.035+0.056BL+0.017NP+0.02NF)( R 2 =0.686);for 100-dph specimens: BW 100 =10 (- 3.742+0.069BL+0. 633 · lg (NP)+0. 464 · lg (NF))( R 2 =0.893);and for 130-dph specimens: BW 130 =10 (- 2.472+0.065BL+0.012NP)( R 2 =0.774). Our work clarified the correlation between various morphological traits and body weight of a commercially-important sea cucumber species ( A . japonicus ). Our predictive models for body weight might be useful for the aquaculture and selective breeding of A . japonicus . These models might also provide theoretical support for the indirect selection of traits that are difficult to select directly.