砂粒粒径与砂层厚度对单体筐养养殖系统中三疣梭子蟹(Portunus trituberculatus)幼蟹摄食行为与生长特性的影响研究

在三疣梭子蟹(Portunus trituberculatus)的单体筐养养殖系统中, 试验了生态掩体(砂盒)中不同的砂粒粒径大小以及砂层厚度对梭子蟹幼蟹摄食行为与生长特性的影响。砂粒粒径设三个水平,分别为: >2mm、<0.2mm 以及混合砂; 砂层厚度有0cm、2cm、5cm、8cm 四个水平。试验共进行6天, 结果表明: 砂粒粒径及砂层厚度对梭子蟹幼蟹的摄食与生长都有明显的影响。从砂粒粒径看, 幼蟹在细砂(SPS)中挖洞休息, 蟹体与砂面呈30—45°角, 仅露眼睛及触角在外。一天内有3—6 次进食,总进食时间为(142.7±22.52)min, 在摄食次数、总进食时间、平均摄食量[(0.2427±0.0137) g/gBW]、以及蜕皮及成活率都远高于其它粒径组, 该组中幼蟹的体重增长最快, 增加了(0.814±0.113)g, 增长率为91.5%±3.43%; 而该组的饵料系数(FCR)最低为1.17±0.11。因此, 筐养养殖系统砂掩体的砂粒粒径最好为0.2 mm 以下。砂层厚度也有类似结果, 5 cm 以上厚度养殖效果最佳。平均摄食量为(0.2087±0.0046) g/gBW, 该厚度下, 幼蟹无死亡、100%蜕皮, 体重也增加最快, 增加了(0.791±0.121)g, 增长率为88.9%±3.74%, 饵料系数(FCR)达到1.37±0.23, 表明筐养系统掩体中砂层厚度要在5 cm 以上。三种保护性酶类(SOD, POD, CAT)活性随着砂粒粒径变小和砂层厚度增加而降低, 而消化酶类(淀粉酶、蛋白酶、脂酶)活性则表现与保护性酶类相反特性。从两类酶的活性变化也能证实, 在优选条件下(细砂、厚度>5 cm), 幼蟹所受的胁迫在降低。     

东海大陆架海域经济蟹类种类组成和数量分布

根据1998年5月、8月、11月和1999年2月东海区虾蟹类资源调查结果,用重量资源密度作为经济蟹类数量分布的数量指标,对东海区经济蟹类的种类组成、数量分布、季节变化趋势以及数量与环境的关系作了定量分析。结果表明,在东海调查海区,经济蟹类主要有细点圆趾蟹Ovalipes punctatus、三疣梭子蟹Portunus trituberculatus、日本蟳Charybdis japonica、锈斑蟳Charybdis feriatus、武士蟳Charybdis miles、光掌蟳Charybdis riversandersoni和红星梭子蟹Portunus sanguinolentus等。经济蟹类渔获量约占蟹类总渔获量的62.8%,渔获量的季节变化明显,年间有二个汛期,一个出现在春夏季,以细点圆趾蟹为主要捕捞对象,兼捕光掌蟳、日本蟳等,渔场的主体分布在东海北部近海,渔场范围较小,蟹群集中,是捕捞生产条件较为理想的渔场;另一个出现在秋冬季,以三疣梭子蟹为主要捕捞对象,兼捕武士蟳、日本蟳、锈斑蟳、红星梭子蟹等。高生物量分布区主要出现在长江口、大沙、舟山渔场20—60m水深海域,是东海三疣梭子蟹和日本蟳的主要渔场,该渔场也是捕捞条件较为理想的渔场。在长江口以南的东海南部海域也有经济蟹类的较高生物量分布区,是武士蟳和锈斑蟳的渔场,该渔场具有范围较大,蟹群较分散的特征,所捕捞的品种往往只能作为兼捕对象。     

快速筑堤技术——鲎式轻质堤坝的结构和筑堤方法

针对软基堤坝稳定性差,容易沉陷滑移,土石方堆不上,工期长,无法快速施工等问题,提出了鲎式轻质堤坝结构和筑堤方法,又称"松垒筑堤法".它减轻了易沉陷部位的质量,均衡堤坝荷重,增强整体稳定性,提高强度,使堤坝与地基溶为一体,消除了堤坝的不均匀沉降;是一种在软弱地基上应用仿生结构的筑堤方法.     

AN EXPERIMENT ON INDUCTION OF TRIPLOIDY IN ERIOCHEIR JAPONICUS HEPUENSIS DAI BY HEAT TREATMENT

Temperature treatment to inhibit extrusion of the polar body of the egg was first used on a lower crustacean, Anemia salina (Gross, 1932), and then was used for inducing triploids of amphibian (Frankhauser and Griffiths, 1939), fish (Svardson, 1945), and mammal (Beatly and Fishchberg, 1949).So far, induction of triploidy has been extensively used to obtain sterile or quick-growth individuals in fish (Swarup, 1956; Lincoln.and Scott, 1983 and Thorgaard, 1986) and mollusk (Stanley et al., 1984), but similar work has not been reported on crab, a higher crustacean.     

乙醇对锯缘青蟹碱性磷酸酶活力与构象的影响

应用荧光光谱、紫外吸收光谱研究锯缘青蟹碱性磷酸乙醇微扰后的分子构象变化情况。乙醇对酶分子构象有显著的影响,酶的内源荧光强度随乙醇浓度增大而增加,荧光发射峰逐渐发生红移,说明酷氨酸、色氨酸残基的微环境发生明显的变化;紫外吸收光谱在278nm吸收峰随乙醇浓度增大而增强。这些结果表明,酶蛋白分子中的生色基因残 基的微环境发生变化;酶的剩余活力随着乙醇浓度增大而迅速下降,经乙醇失活的酶在正常的测活体系中监测酶活力,发现酶活力可以回升到92％以上,说明酶的乙醇失活是可逆的,可以通过稀释复活。测定乙醇对酶的抑制动力学,表明乙醇对青蟹碱性磷酸酶的抑制作用是反竞争性机制,其抑制常数Ki为11．8％。     

饲养空间大小对单体筐养养殖系统中三疣梭子蟹(Portunus trituberculatus)的摄食行为与生长特性的影响研究

在三疣梭子蟹(Portunus trituberculatus)的单体筐养养殖(SCBC)系统中,研究了不同的饲养空间大小对梭子蟹(幼蟹与成蟹)摄食行为与生长的影响。试验设置了四个饲养空间处理:空间长度分别为养殖蟹平均壳长的1倍、1.5倍、2倍及3倍,宽为长的一半。经6天试验,结果表明,1倍空间的幼蟹摄食行为发生变化,摄食次数[(1.2±0.45)次/d]和总进食时间[(31.2±6.89)min]比2倍与3倍的空间[(2.6±0.45)次/d,(83.1±13.66)min]明显减少,导致摄食量减少[前者为(0.0835±0.0269)g/g BW,后两者最高为(0.2014±0.0114)g/g BW],至第5天全部死亡。其它处理,随着空间的增大,摄食量增大,幼蟹的生长速率(蜕皮率,体重增长率)增加。其中,在2倍空间以上处理中,幼蟹表现有最高的成活率(100%)、蜕皮率(100%)以及体重增长率(86.5%—97.2%),同时有较低的饵料系数(1.31±0.11)。成蟹的试验结果与幼蟹类似,除1.5倍饲养空间外,该处理中幼蟹表现出对生长的胁迫,而成蟹的摄食行为和生长与2倍及3倍空间无差异。不同空间中梭子蟹的摄食与生长的差异与梭子蟹在这些处理中的保护性酶类(SOD,POD,CAT)和消化酶类(淀粉酶,蛋白酶,脂酶)的活性有密切关系。     

Changes during late-stage embryonic development from egg-juvenile to free-living hatchling in Chinese freshwater crab Sinopotamon yangtsekiense (Decapoda, Brachyura, Potamidae)

This study expands on recent reports that direct development in the Chinese potamid freshwater crab Sinopotamon yangtsekiense involves the completion of all brachyuran larval stages (nauplius, zoea, and megalopa) inside the egg case during embryonic development. Detailed studies of embryonic development in this species revealed the presence of an additional larval stage (the egg-juvenile) between the megalopa and the free-living hatchling crab. We described and compared the appendages of the head, thorax, and abdomen of the egg-juvenile with those of the hatchling crab in S. yangtsekiense. Significant differences were found between most of the appendages of these two stages with a soft exoskeleton in the egg-juvenile, no joint articulation, a slimmer appearance, and a lack of setae when compared with the newly emerged free-living hatchling crab. These modifications of the appendages are related to the confinement within the egg case of the egg-megalopa and egg-juvenile during direct development, and the need for the free-living hatchling freshwater crab to move, feed, and respire. In marine crabs, the megalopa gives rise to the first crab stage whereas in freshwater crabs the egg-juvenile follows the megalopa and immediately precedes the free-living first crab stage.     

10种蟹类18S rDNA 和COⅠ基因序列分析及其分子系统发育研究

应用分子系统发育学的方法,以蟹类18S rDNA和线粒体细胞色素C氧化酶亚单位Ⅰ(COⅠ)基因序列片段为分子标记,结合形态学特征对10种蟹类的分类地位进行探讨。实验共获得10 条18S rDNA序列,长度为1780~1787 bp,其中A、T, G,C平均含量分别为23.72%, 24.58%,24.52%和27.17%;通过序列对比,发现18S rDNA序列相对保守,只含有1个从88 bp 到130 bp 约 50 bp的高可变区;物种间碱基距离比较小,从0.001到0.017。18S rDNA系统发育树为方蟹总科、沙蟹总科和梭子蟹总科起源于同-海洋蟹类祖先提供了分于生物q证惦,开上火亚N内尔量分别为26.88%.弓蟹科。获得的5条CO Ⅰ基因序列,长度均为709 bp,A、T,G、C平均含量分别为26.88%,37.62%,17.50%和18.00%;COⅠ基因片段变异性高,物种间碱基距离从0.016到0.138。COⅠ基因系统发育树真实地反映了住属各物种之间的进化关系,和传统分类非常吻合,为形态特征相似的姆类鉴定提供了-种快速准确的方法。     

三疣梭子蟹(Portunus trituberculatus)幼蟹饲料中胆固醇的适宜添加量研究

采用单因素实验设计方法, 以初始体重为20g左右的三疣梭子蟹(Portunus trituberculatus)幼蟹为实验对象, 胆固醇添加量为0.003%、0.3339%、0.655%、0.986%、1.303%和1.628%的实验饲料投喂8周, 研究其胆固醇需求量。结果表明, 饲料中添加0.003%—1.628%胆固醇对各处理组的存 活率无显著性影响(P>0.05)。随饲料胆固醇水平的上升, 三疣梭子蟹幼蟹的增重率呈先上升后下降的趋势, 当饲料胆固醇水平≥0.655%时, 三疣梭子蟹幼蟹的增重率呈下降趋势。折线模型表明, 饲料中胆固醇添加量为0.6%时, 三疣梭子蟹幼蟹的增重率、性腺指数和壳宽增加达到最高值。根据增重率折线模型结果, 作者认为三疣梭子蟹幼蟹饲料中添加0.6%左右胆固醇较为适宜     

Effects of habitat complexity on cannibalism rates in European green crabs (Carcinus maenas Linnaeus, 1758)

The habitat in which predator–prey interactions take place may have a profound influence on the outcome of those interactions. Cannibalism is an intriguing form of predation whereby foraging by predators may contribute to the regulation of their own populations.This is particularly interesting in the case of invasive species, like the widely distributed European green crab (Carcinus maenas). This study explores how habitat complexity influences cannibalism rates in green crab populations of Prince Edward Island, Atlantic Canada. Both laboratory and field experiments were conducted to measure feeding rates by individual adult green crabs on a standard number of smaller conspecifics. In the laboratory, experimental treatments mimicked unstructured to increasingly structured habitats: water, sandy bottom, oyster shells, mussel shells, oyster shells with sandy bottom and mussel shells with sandy bottom. In those trials, adult green crabs consumed several times more juveniles on unstructured habitats than on the most structured ones, with a gradual decrease in predation rates across increasingly complex habitats. Field inclusion experiments used the same approach and were conducted in sandy bottoms, sandy bottoms with a layer of oyster shells and sandy bottoms with a layer of mussel shells. These trials showed similar patterns of decreasing feeding rates across increasingly complex habitats, but differences among treatments were not significant. These results support the idea that complex habitats have the potential to mediate predator–prey interactions, including adult–juvenile cannibalism in green crabs.