鳜鱼(Siniperca chuatsi)脂肪酸去饱和酶和延长酶基因全长cDNA序列的克隆与分析

利用RT-PCR和RACE方法克隆得到鳜鱼肝脏中控制高不饱和脂肪酸合成的脂肪酸去饱和酶(fatty acid desaturase,FAD)和脂肪酸延长酶(fatty acid elongase,ELO)基因的全长cDNA序列。FAD基因的全长cDNA序列1882bp,编码445个氨基酸,该蛋白序列含有FAD全部的特征结构区,包括3个组氨酸簇、2个跨膜区和1个细胞色素b5结构域,与其它鱼类FAD氨基酸序列的同源性为66.5%-90.1%,系统树分析显示其与欧洲鲈鱼和金头鲷等海水鱼类的亲缘关系最近。获得的ELO基因全长cDNA序列1401bp,编码294个氨基酸,该蛋白序列含有单一的氧化还原中心组氨酸簇、内质网停留信号和多个跨膜区等ELO特征结构,与其它几种鱼类ELO氨基酸序列的同源性为71.8%-86.7%,系统进化分析显示其与南方蓝鳍金枪鱼和金头鲷的亲缘关系较近。鳜鱼FAD和ELO基因全长cDNA序列的获得可为进一步研究其HUFA合成能力及调控机理奠定基础。     

Miocene facies associations and sedimentary evolution of the Southern Transylvanian Basin (Romania): Implications for hydrocarbon exploration

The Transylvanian Basin is a mature hydrocarbon province of Romania characterized by two petroleum systems: Mesozoic (thermogenic) and Miocene (biogenic). An extensive outcrop-based sedimentological and micropaleontological study correlated to seismic and well data discusses the elements of the Miocene petroleum system. The facies associations are indicative of alluvial, fandelta, shallow- and deep-marine settings. These are grouped into four different depositional systems (evaporite, mud-carbonate, sand-mud and sand-gravel). Their evolution in time and space shows large differences between various parts of the basin that have important consequences for exploration.     

Seismic stratigraphy and subsidence analysis of the southern Brazilian margin (Campos,Santos and Pelotas basins)

The Campos, Santos and Pelotas basins have been investigated in terms of 2D seismo-stratigraphy and subsidence. The processes controlling accommodation space (e.g. eustacy, subsidence, sediment input) and the evolution of the three basins are discussed. Depositional seismic sequences in the syn-rift Barremian to the drift Holocene basin fill have been identified. In addition, the subsidence/uplift history has been numerically modeled including (i) sediment flux, (ii) sedimentary basin framework, (iii) relation to plate-tectonic reconfigurations, and (iv) mechanism of crustal extension. Although the initial rift development of the three basins is very similar, basin architecture, sedimentary infill and distribution differ considerably during the syn-rift sag to the drift basin stages. After widespread late Aptian–early Albian salt and carbonate deposition, shelf retrogradation dominated in the Campos Basin, whereas shelf progradation occurred in the Santos Basin. In the Tertiary, these basin fill styles were reversed: since the Paleogene, shelf progradation in the Campos Basin contrasts with overall retrogradation in the Santos Basin. In contrast, long-term Cretaceous–Paleogene shelf retrogradation and intense Neogene progradation characterize the Pelotas Basin. Its specific basin fill and architecture mainly resulted from the absence of salt deposition and deformation. These temporally and spatially varying successions were controlled by specific long-term subsidence/uplift trends. Onshore and offshore tectonism in the Campos and Santos basins affected the sediment flux history, distribution of the main depocenters and occurrence of hydrocarbon stratigraphic–structural traps. This is highlighted by the exhumation and erosion of the Serra do Mar, Serra da Mantiqueira and Ponta Grossa Arch in the hinterland, as well as salt tectonics in the offshore domain. The Pelotas Basin was less affected by changes in structural regimes until the Eocene, when the Andean orogeny caused uplift of the source areas. Flexural loading largely controlled its development and potential hydrocarbon traps are mainly stratigraphic.     

中国沿海10 种方蟹16S rRNA 基因序列分析及系统发育研究

中国沿海10种方蟹线粒体16S rRNA基因部分片段的序列长度为517 bp～533 bp。它们的核苷酸序列A、T、G、C的含量相似,A+T的含量(69.8%～76.0%)明显高于G+C的含量;10种方蟹的16S rRNA基因序列比对获得541 bp的同源序列(含插入/缺失位点),除插入/缺失位点外共检测到146个变异位点,其中81个为简约信息位点。4种厚蟹与2种近方蟹的遗传距离(0.054～0.085)都显著小于与其他方蟹之间的遗传距离,甚至明显小于与4种厚蟹原本属于同一相手蟹科的2种相手蟹之间的遗传距离(0.105～0.155);而基于16S rRNA基因片段序列采用NJ法构建的系统进化树的拓扑结构也显示,原本属于相手蟹科的侧足厚蟹、天津厚蟹、日本仿厚蟹和伍氏仿厚蟹没有与2种相手蟹聚为一支,而是最终与属于弓蟹科的2种近方蟹聚为一大支,且有高达99%的支持率。结果表明,4种厚蟹与2种近方蟹的亲缘关系相对较近,而与2种相手蟹等其他方蟹的亲缘关系则相对较远。因此,研究结果支持将4种厚蟹从相手蟹科移到弓蟹科。此外,属于相手蟹科的2种相手蟹聚为一支,属于方蟹科的白纹方蟹和属于斜纹蟹科的瘤突斜纹蟹又各自成为一支;表明16S rRNA基因的分子数据支持其形态学分类结果的正确性,提示上述4科蟹类可能分别为单系。     

层序地层学模拟研究进展及趋势

模拟层序地层学是层序地层学理论重要的研究手段之一,在国外该项技术较为成熟.层序地层计算机模拟软件采用的算法主要有扩散算法、流体流动算法、几何控制沉积算法、经验数值逼近算法、沉积物搬运的构造校正算法.目前,国外学者对以海平面升降、构造沉降和沉积物供应为主控因素的层序地层概念模拟的适用性提出了质疑,并对层序控制因素的多样性和不确定性进行了深入的研究.结合当前的研究进展,提出了层序控制因素的"多变量系统"观点,将层序的控制因素分为综合变量和独立变量,进一步指出如果假设综合变量为独立变量,则可以简化层序模拟,假设越多,模拟越简单,但模拟结果越粗略;要使模拟结果真实地反映实际地层特征,就要尽可能采用独立变量.     

Late Quaternary stratigraphy and sedimentology of the inner part of southwest Joseph Bonaparte Gulf

Joseph Bonaparte Gulf is a large embayment on the northwestern continental margin of Australia. It is approximately 300 km east‐west and 120 km north‐south with a broad continental shelf to seaward. Maximum width from the southernmost shore of Joseph Bonaparte Gulf to the edge of the continental shelf is 560 km. Several large rivers enter the gulf along its shores. The climate is monsoonal, sub‐humid, and cyclone‐prone during the December‐March wet season. A bedrock high (Sahul Rise) rims the shelf margin. The sediments within the gulf are carbonates to seaward, grading into clastics inshore. A seaward‐thinning wedge of highstand muds dominates the sediments of the inner shelf of Joseph Bonaparte Gulf. Mud banks up to 15m thick have developed inshore. Coarse‐grained sand ridges up to 15 m high are found off the mouth of the Ord River. These overlie an Upper Pleistocene transgressive lag of mixed carbonate and gravelly siliciclastic sand. Four drowned strandlines are present on the inner shelf at depths of 20, 25, 28 and 30 m below datum. These are interpreted as having formed during stillstands in the Late Pleistocene transgression. Older strandlines at great depths are inferred as having formed during the fall in sea‐level following the last highstand. For the most part the Upper Pleistocene‐Holocene marine sediments overlie an erosion surface cut into older Pleistocene sediments. Incised valleys cut into this erosion surface are up to 5 km wide and have a relief of at least 20 m. The largest valley is that cut by the Ord River. Upper Pleistocene sediments deposited in the incised valleys include interpreted lowstand fluvial gravels, early transgressive channel sands and floodplain silts, and late transgressive estuarine sands and gravels. Older Pleistocene sediments are inferred to have been deposited before and during the 120 ka highstand (isotope stage 5). They consist of sandy calcarenites deposited in high‐energy tide‐dominated shelf environments. Still older shelf and valley‐fill sediments underlie these. The contrast between the Holocene muddy clastic sediments and the sandy carbonates deposited by the 120 ka highstand suggests that either the climate was more arid in the past, with less fluvial transport, or that mud was more effectively trapped in estuaries, allowing development of carbonate depositional environments inshore.     

Constraining sequence stratigraphy in north Australian basins: SHRIMP U–Pb zircon geochronology between Mt Isa and McArthur River

Fifty‐five new SHRIMP U–Pb zircon ages from samples of northern Australian ‘basement’ and its overlying Proterozoic successions are used to refine and, in places, significantly change previous lithostratigraphic correlations. In conjunction with sequence‐stratigraphic studies, the 1800–1580 Ma rock record between Mt Isa and the Roper River is now classified into three superbasin phases—the Leichhardt, Calvert and Isa. These three major depositional episodes are separated by ～20 million years gaps. The Isa Superbasin can be further subdivided into seven supersequences each 10–15 million years in duration. Gaps in the geological record between these supersequences are variable; they approach several million years in basin‐margin positions, but are much smaller in the depocentres. Arguments based on field setting, petrography, zircon morphology, and U–Pb systematics are used to interpret these U–Pb zircon ages and in most cases to demonstrate that the ages obtained are depositional. In some instances, zircon crystals are reworked and give maximum depositional ages. These give useful provenance information as they fingerprint the source(s) of basin fill. Six new ‘Barramundi’ basement ages (around 1850 Ma) were obtained from crystalline units in the Murphy Inlier (Nicholson Granite and Cliffdale Volcanics), the Urapunga Tectonic Ridge (‘Mt Reid Volcanics’ and ‘Urapunga Granite’), and the central McArthur Basin (Scrutton Volcanics). New ages were also obtained from units assigned to the Calvert Superbasin (ca 1740–1690 Ma). SHRIMP results show that the Wollogorang Formation is not one continuous unit, but two different sequences, one deposited around 1730 Ma and a younger unit deposited around 1722 Ma. Further documentation is given of a regional 1725 Ma felsic event adjacent to the Murphy Inlier (Peters Creek Volcanics and Packsaddle Microgranite) and in the Carrara Range. A younger ca 1710 Ma felsic event is indicated in the southwestern McArthur Basin (Tanumbirini Rhyolite and overlying Nyanantu Formation). Four of the seven supersequences in the Isa Superbasin (ca 1670–1580 Ma) are reasonably well‐constrained by the new SHRIMP results: the Gun Supersequence (ca 1670–1655 Ma) by Paradise Creek Formation, Moondarra Siltstone, Breakaway Shale and Urquhart Shale ages grouped between 1668 and 1652 Ma; the Loretta Supersequence (ca 1655–1645 Ma) by results from the Lady Loretta Formation, Walford Dolomite, the upper part of the Mallapunyah Formation and the Tatoola Sandstone between ca 1653 and 1647 Ma; the River Supersequence (ca 1645–1630 Ma) by ages from the Teena Dolomite, Mt Les and Riversleigh Siltstones, and Barney Creek, Lynott, St Vidgeon and Nagi Formations clustering around 1640 Ma; and the Term Supersequence (ca 1630–1615 Ma) by ages from the Stretton Sandstone, lower Doomadgee Formation and lower part of the Lawn Hill Formation, mostly around 1630–1620 Ma. The next two younger supersequences are less well‐constrained geochronologically, but comprise the Lawn Supersequence (ca 1615–1600 Ma) with ages from the lower Balbirini Dolomite, and lower Doomadgee, Amos and middle Lawn Hill Formations, clustered around 1615–1610 Ma; and the Wide Supersequence (ca 1600–1585 Ma) with only two ages around 1590 Ma, one from the upper Balbirini Dolomite and the other from the upper Lawn Hill Formation. The Doom Supersequence (<1585 Ma) at the top of the Isa Superbasin is essentially unconstrained. The integration of high‐precision SHRIMP dating from continuously analysed stratigraphic sections, within a sequence stratigraphic context, provides an enhanced chronostratigraphic framework leading to more reliable interpretations of basin architecture and evolution.     

The Arunta Inlier: a complex ensialic mobile belt in central Australia. Part 1: Stratigraphy,correlations and origin

The Arunta Inlier is a 200 000 km² region of mainly Precambrian metamorphosed sedimentary and igneous rock in central Australia. To the N it merges with similar rocks of lower metamorphic grade in the Tennant Creek Inlier, and to the NW it merges with schist and gneiss of The Granites‐Tanami Province. It is characterized by mafic and felsic meta‐igneous rocks, abundant silicic and aluminous metasediments and carbonate, and low‐ to medium‐pressure metamorphism. Hence, the Arunta Inlier is interpreted as a Proterozoic ensialic mobile belt floored by continental crust. The belt evolved over about 1500 Ma, and began with mafic and felsic volcanism and mafic intrusion in a latitudinal rift, followed by shale and limestone deposition, deformation, metamorphism and emergence. Flysch sedimentation and volcanism then continued in geosynclinal troughs flanking the ridge of meta‐igneous rocks, and were followed by platform deposition of thin shallow‐marine sediments, further deformation, and episodes of metamorphism and granite intrusion.     

事件地层学与地外灾变事件

从P∈—∈,P—T等地层界线上的特征岩石、古生物大规模灭绝和Ir及ξ~(13)C的异常等研究表明,与K—R界线一样,在这些界线上也可能存在有地外灾变事件。同时文中提出了界线灾变事件、界线灾变期、界线灾变环境、界线层、界线粘土层、梅树村地质事件等与研究事件地层学有关的一系列新概念。     

Sequence stratigraphy in the upper cretaceous of the Basco-Cantabrian Basin (northern Spain)

The sedimentary cycles of the Cenomanian to Maastrichtian were investigated in the Basco-Cantabrian Basin (BCB) in northern Spain (Provinces of Alava, Vizcaya and Burgos). The depositional area was a distally steepened carbonate ramp which extended from Catalonia northwestwards to the Basque country. The investigated sediments range from calciturbidites and pelagic marls to marl-limestone alternations deposited on a distal carbonate ramp. Shallow marine limestones, marls and intertidal clastics and carbonates were deposited on the proximal part of the carbonate ramp. The establishment of a regional sequence analysis is based on the investigation of seismic profiles, well logs and outcrop sections. Examples of outcrop sections are interpreted in terms of sequence stratigraphy (unconformities of third- and second-order cycles, depositional geometries, systems tracts). The sequence stratigraphic interpretation of outcrop sections is based on facies analysis, interpretation of observed depositional geometries and correlation of unconformities and marine flooding surfaces through the basin. A biostratigraphic framework is established based on ammonites, inoceramids, planktonic and benthic foraminifera. As a result, a regional sequence stratigraphic cycle chart is presented and compared with published global cycle charts. The correlation of the regional cycle chart with published cycle charts is good. In the Cenomanian and Turonian, several sequence boundaries in the BCB are shifted by up to one biozone compared with the global chart. Some type 1 boundaries of the standard chart are only type 2 in the BCB. Important type 1 boundaries in the BCB are: top Geslinianum Zone with a 100 m lowstand wedge at the basis of the sequence (sequence boundary 92.2) ; base Petrocoriense Zone with a 250 m shallowing-upwards lowstand wedge at the basis (sequence boundary 89.2); and within the Syrtale Zone (sequence boundary 85.0).The Campanian-Maastrichtian sequence record is strongly disturbed by local compressive tectonics. Several sequences are recognizable and can be correlated with the global cycle chart. Correlation is hampered by the low biostratigraphic resolution in the western basin part. Subsidence analysis of several sections of the Upper Cretaceous of the BCB and its interpretation in the regional tectonic context leads to a discussion of the causes of the observed cyclicity. A regional eustatic curve is presented for the Upper Cretaceous of the BCB. Stage and substage names were used according Code-Committee (1977). Correspondence to: K.-U. Gräfe