高产赤霉素菌株选育

针对赤霉素工业生产菌株皆为多核多细胞菌丝型,不产生孢子的特性,首先将赤霉素菌丝体通过酶解手段,溶去其细胞壁使其成为单一细胞游离的原生质体,并在此基础上通过紫外线等理化诱变,使其产生基因突变,然后在再生平板上,使赤霉素原生质体中的突变型菌株得到再生,最后通过摇瓶筛选,得到一株生产水平高、生产性能稳定的高产赤霉素菌株。     

海藻提取物抗炎活性的筛选

分属绿藻门、红藻门、褐藻门的海藻样品于2000年采于山东青岛和威海沿海，首次采用人白细胞弹性蛋白酶体外筛选技术对39种海藻样品的甲醇提取物进行了抗炎活性筛选，以期从海藻中寻找天然抗类风湿性关节炎等炎症性疾病的药物活性前体。结果表明，18种海藻（7种褐藻，11种红藻）对HLE有抑制活性，而绿藻缺乏抗HLE活性。这18种海藻中，7种表现出较强的抑制活性，分别为：鸭毛藻、海头红、江蓠、细枝软骨藻、粗枝软骨藻、小粘膜藻和绳藻，其中尤以绳藻的抑制活性最强（在浓度为25μg/ml时可达98．9％）。     

湖光岩拮抗菌的研究

采用涂布法、划线分离法以及形态学和生理生化特性分析,对湛江湖光岩微生物进行了分离纯化及部分性质研究;同时用平板菌落计数法对其异养细菌、真菌和弧菌等的数量进行初步统计。采用牛津杯法,对拮抗细菌进行筛选和培养特性研究。结果表明,微生物数量与种类在水平分布上具有显著差异。底泥异养菌总量为（1．3—8．6）×10^8 cfu／g,真菌总量为（0．1—5．7）×10^7 cfu／g。而水体细菌总量为（0．5—6．7）×10^5 cfu／ml,真菌总量为（0．4-1．1）×10^3 cfu／ml。共分离纯化到148株细菌,经筛选发现16株细菌对溶藻弧菌（Vibrio alginolyticus）具有明显的抑制作用,占细菌总数的10．8％,其中菌株DH078和DH113对溶藻弧菌的拈抗作用最明显。结果还表明,细菌DH078在营养肉汤中培养4d抑菌活性最强,其产生抗菌活性物质的最佳条件是稀释2倍的营养肉汤、蛋白胨0．6％、牛肉膏0．6％、酵母膏0．2％、盐度0和pH7．0。可见湖光岩微生物资源较为丰富,本研究可为保护和充分开发利用湖光岩微生物提供科学依据。     

一个计算山地地形参数的计算机模式

本文提出了一个虚拟次网格二维差分格式,依此建立了一个计算山地小地形参数(即局地平均坡向、坡度和地形遮蔽角)的通用计算机模式。模式的输入参数为研究地区的拔海高度网格化后的资料、空间格距和网格点数;模式的输出结果为每个格点上的坡向、坡度及沿任一方位上的地形遮蔽角以及一些地形统计值。由此,可较准确、客观地制作出研究山区的坡向、坡度图和地形遮蔽图。经实测验证,模式的计算值与实测值比较吻合。     

海洋源生防细菌LHB02的筛选、鉴定及其抑菌谱检测

从高度污染的海滩污泥中,分离得到一株对部分水产病原菌有很强拮抗作用的生防细菌LHB02。采用细菌的通用引物进行LHB02的16SrRNA基因片段扩增,得到l511bp的DNA片段,其序列与枯草芽孢杆菌（Bacillus subtilis）在进化位置上最为接近,结合传统的形态学、生理生化鉴定手段,鉴定该菌株为枯草芽孢杆菌...     

Amplitude reduction of elastic waves by a row of piles in poroelastic soil

This paper studies the displacements behind a row of elastic piles in a homogeneous unbounded poroelastic soil after excitation by the passage of elastic waves. Biot’s theory is employed to describe soil behavior. Using a Fourier–Bessel series expansion with the aid of a translational addition theorem, closed-form expressions for the scattering coefficients are developed by imposing continuity conditions at the pile–soil interfaces. The influence of certain parameters, such as pile rigidity and soil permeability on the screening performance of the pile barrier is investigated. Particular attention is focused on the influence of the soil permeability on the screening effectiveness of a row of piles in the frequency domain.     

Screening of biosurfactant producers from petroleum hydrocarbon contaminated sources in cold marine environments

An overview of literature about isolating biosurfactant producers from marine sources indicated no such producers have been reported form North Atlantic Canada. Water and sediment samples were taken from petroleum hydrocarbon contaminated coastal and offshore areas in this region. Either n-hexadecane or diesel was used as the sole carbon source for the screening. A modified colony-based oil drop collapsing test was used to cover sessile biosurfactant producers. Fifty-five biosurfactant producers belong to genera of Alcanivorax, Exiguobacterium, Halomonas, Rhodococcus, Bacillus, Acinetobacter, Pseudomonas, and Streptomyces were isolated. The first three genera were established after 1980s with interesting characteristics and limited relevant publications. Some of the 55 isolated strains were found with properties such as greatly reducing surface tension, stabilizing emulsion and producing flocculant. Isolates P6-4P and P1-5P were selected to demonstrate the performance of biosurfactant production, and were found to reduce the surface tension of water to as low as 28 dynes/cm.