Peculiar nature of hard X-ray eclipse in SS433 from INTEGRAL observations

The analysis of hard X-ray INTEGRAL observations (2003–2008) of superaccreting Galactic microquasar SS433 at precessional phases of the source with the maximum disc opening angle is carried out. It is found that the shape and width of the primary X-ray eclipse are strongly variable, suggesting additional absorption in dense stellar wind and gas outflows from the optical A7I component and the wind–wind collision region. The independence of the observed hard X-ray spectrum on the accretion disc precessional phase suggests that hard X-ray emission (20–100 keV) is formed in an extended, hot, quasi-isothermal corona, probably heated by interaction of relativistic jet with inhomogeneous wind outflow from the precessing supercritical accretion disc. A joint modelling of X-ray eclipsing and precessional hard X-ray variability of SS433 revealed by INTEGRAL by a geometrical model suggests the binary mass ratio   q = m_x / m _v ≃  0.25–0.5. The absolute minimum of joint orbital and precessional  χ²  residuals is reached at   q ≃ 0.3  . The found binary mass ratio range allows us to explain the substantial precessional variability of the minimum brightness at the middle of the primary optical eclipse. For the mass function of the optical star   f _v = 0.268 M_⊙  as derived from Hillwig & Gies data, the obtained value of   q ≃ 0.3  yields the masses of the components   m_x ≃ 5.3 M_⊙, m _v ≃ 17.7 M_⊙  , confirming the black hole nature of the compact object in SS433.     

龙感湖水体光学特性的观测

基于2002—2003年秋、夏季原位水下光场巡测及连续定点观测资料分析了龙感湖不同湖区及不同风浪条件下水体的光学特性,探讨了光衰减系数、辐照度比的光谱分布、空间差异及不同风浪条件对水下光场的影响.结果表明,水下光谱在紫光波段衰减最强烈,其次是蓝光,红、绿光衰减最弱,并且向下辐照度衰减系数一般要大于向上辐照度衰减系数.秋季L1-L3点向下辐照度400—700nm波段衰减系数的变化范围分别为0.71—3.60、1.06—3.72、0.78—2.89m-1;光衰减系数的空间分布是位于湖中心的12点要略大于两边的L1、L3点;辐照度比的变化趋势极为一致,最低值出现在短波蓝光波段,最高值出现在550—600nm之间;从小风浪到中风浪、大风浪其PAR衰减系数分别是1.74、2.02、2.45m-1;透明度、衰减系数与悬浮物浓度相关性最好,决定系数在0.7以上,但其变化除受悬浮物影响外还要受制于溶解性有机物和浮游植物;440nm波长衰减系数(Kd(440))与悬浮物(SS)、溶解性有机碳(DOC)、叶绿素a(Chl.a)的多元线性回归方程为:Kd(440)=0.514—0.075SS 0.125DOC 0.100Chl.a(R2=0.87,N=8,P≤0.05)     

溶藻弧菌Ⅲ型分泌系统注射装置蛋白VscO的原核表达及免疫原性

为探究溶藻弧菌(Vibrio alginolyticus)ZJ03株Ⅲ型分泌系统(Type III secretion system,T3SS)注射装置蛋白VscO作为疫苗候选抗原的可能性,根据GeneBank上登陆的溶藻弧菌VscO序列(NO.KJ179947),设计1对带酶切位点的特异性引物,PCR扩增vscO基因,序列分析结果显示,该基因全长462 bp,理论分子质量为18.430ku。将vscO基因定向插入原核表达载体pET-28a(+),构建重组表达质粒pET-vscO。用异丙基-β-D-硫代半乳糖苷(IPTG)诱导后,可在大肠杆菌(Escherichia coli)BL21(DE3)中表达分子质量约为22 ku的VscO融合蛋白,且该蛋白主要以包涵体形式存在。VscO蛋白表达和纯化的最优条件为:0.1 mmol/L IPTG、37℃条件下诱导4 h,咪唑洗脱浓度为400 mmol/L。用纯化后的融合蛋白免疫SPF级小鼠,获得高效多克隆抗体。Western-blotting结果表明,鼠抗VscO血清既能与重组VscO蛋白发生反应,也能与分离自溶藻弧菌约22 ku的天然蛋白发生反应,提示T3SS注射装置蛋白VscO可能是溶藻弧菌的重要保护性抗原之一。