Development of a real-time PCR method for Thalassiosira rotula rapid detection

Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.     

深水油气田井场调查技术研究

随着近海油气不断开发，其后续发展能力明显不足，因此深水含油气盆地的开发将成为必然的发展趋势。深水油气田的井场调查是深水油气田开发过程中的一个主要环节，其勘探技术越来越被人们所关注。本文通过深水井场调查的技术要求分析，结合多次组织和参加近海井场调查的工作经验，指出深水油气田井场调查的技术难点在于用于探索海底表面障碍物分布情况、勘测海底地形地貌特征、了解中浅地层结构等这类勘探技术上。由于这些勘探技术受声纳技术特点的限制，国外已经采用了DEEFTOW、ROV或者AUV技术，将一些关键调查设备与海底保持一定的高度来实现勘探技术目标，并已经取得成功的尝试，因此，研究、发展和不断地完善这类贴近海底多参量勘查技术十分重要。     

A General Linear Wave Theory for Water Waves Propagating over Uneven Porous Bottoms

Starting from the widespread phenomena of porous bottoms in the near shore region, considering fully the diversity of bottom topography and wave number variation, and including the effect of evanescent modes, a general linear wave theory for water waves propagating over uneven porous bottoms in the near shore region is established by use of Green‘s scond identity. This theory can be reduced to a number of the most typical mild-slope equations curreutly in use and provide a reliable research basis for follow-up development of nonlinear water wave theory involving porous bottoms.     

Mach Effect of Oblique Nonlinear Irregular Waves Interacting with Vertical Walls

Based on Hong‘s theory, previous random models, and a generalized expression suitable for FIT calculation, the interaction between irregular waves and vertical walls is numerically simulated. The results of simulation demonstrate that the wave energy changes with the incidence angle and the distance from the wall. Particularly, the Mach effect and the combined wave spectrum characteristics are analyzed in detail, which are significant in both theory and practice.     

Probabilistic Models for the Probability of Wave Breaking and Whitecap Coverage Based on Kinematic Breaking Criterion

More and more researches show that neither the critical downward acceleration nor the critical slope of water waves is a universal constant. On the contrary, they vary with particular wave conditions. This fact moders the models either for the probability of wave breaking B or for the whitecap coverage W based on these criteria difficult to apply. In this paper and the one which follows we seek to develop models for the prediction of both B and W based on the kinematical criterion. First, several joint probabihstic distribution functions (PDFs) of wave characteristics are derived, based on which the breaking properties B and W are estimated. The estimation is made on the assumption that a wave breaks ff the horizontal velocity of water particles at its crest exceeds the local wave celerity, and whitecapping occurs in regions of fluid where water particles travel faster than the waves. The consequent B and W depend on wave spectral moments of orders 0 to 4.Then the JONSWAP spectrum is used to represent the fetch-limited sea waves in deep water, so as to relate the probahility of wave breaking and the whitecap coverage with wind parameters. To this end, the time-averaging technique proposed by Glazman (1986) is applied to the estimation of the spectral moments involved, and furthermore, the theoretical models are compared with available observations collected from published literature. From the comparison, the averaging time scale is determined. The final models show that the probability of wave breaking as well as the whitecap coverage depends on the dimensionless fetch. The agreement between these models and the database is reasonable.     

基于塑性极限分析理论和神经网络响应面的结构系统可靠性分析

根据多层神经网络映射存在定理，提出了基于神经网络响应面和塑性极限分析理论的结构系统可靠性分析的新方法。数值试验表明，该方法可以快速、高质量地求出结构系统可靠性指标，是一个具有发展前景的新方法。     

香港附近海区台风波浪的波群统计分析

根据香港附近海区１９８５，１９８６年两次台风大浪的实测资料，以有效波高为相应水平，对波群作统计分析，得出可供海洋工程参考的波群连长、波群重复长度、波群出现频率以及波群中最大波高与有效波高的关系等。并将统计值与理论值作比较，结果表明：统计值大于“连”的理论值，而与包线理论中采用合田修正公式的汁算值相近。     

不规则波折射物理模型试验研究

作者曾发表过“不规则波折射的数学模型〔１〕。为了与数学模型的计算结果相互验证，在大连理工大学海岸及近海工程国家重点实验室进行了不规则波折射物理模型试验。试验研究结果表明：提出的“不规则波折射数学模型”对频谱采用等能量分割、对方向分布采用等间隔（Δθ＝ｃｏｓｎｔ）分割的组成波折射线性叠加法是合理的、可行的，可供港口及海岸工程规划、设计应用     

地学组织面临的新挑战——CCOP专题会议报告

“地学组织面临的新挑战以及对其现状和组织结构的影响”专题会议于 2 0 0 1年 1 0月 2 6日在柬埔寨首都金边与 CCOP第 3 8届年会同时召开。来自 CCOP成员国和合作国 /组织的代表出席了会议。会议由 CCOP名誉顾问 YoshihikoShimazaki博士和何起祥教授主持 ,中国的蒋仕金先生被选为本报告的撰写人。参加会议的有柬埔寨、中国、印度尼西亚、日本、马来西亚、泰国、越南和菲律宾的代表 ,也有来自德国、瑞典、得克萨斯农工大学和荷兰的代表。虽然各国和地学研究状况不同 ,但是整个地区的地学组织面临的挑战是共同的。 CCOP成员国为了满…     

地震折射波的虚拟速度—临界角谱的原理与制作方法

虚拟速度-临界角谱是地震折射波虚拟射线理论的应用之一。根据共始点或共折射点记录的相似性，利用第一虚拟时距曲线方程，对时距曲线进行扫描计算，可以制作共始点谱和共折射点谱。文中，通过实际介质模型的计算机试算结果，对该方法作了验证。