饲料中添加益生菌对凡纳滨对虾(Litopenaeus vannamei)非特异免疫基因表达量和抗病力的影响

采用实时荧光定量PCR方法,进行肠道复合益生菌对凡纳滨对虾相关免疫基因表达量的研究。通过感染实验,分析饲料中添加益生菌对凡纳滨对虾病毒感染能力的影响。结果表明,Real-time PCR揭示在投喂期间饲料中添加芽孢杆菌/溶藻弧菌组的对虾血淋巴细胞中的proPO、SOD和LZM的相对表达量均显著高于对照组(P<0.05);感染WSSV后,对芽孢杆菌/溶藻弧菌组凡纳滨对虾血淋巴7个采样时间点的proPO、SOD、LZM的表达进行Real-time PCR分析,检测表明WSSV刺激24h后,对虾血淋巴中的proPO、SOD和LZM的表达呈现显著性上调,且分别在48h、96h、96h达到最大值。累积死亡率结果证实饲料中添加益生菌均可提高其对虾抗WSSV感染的能力,尤其以坚强芽孢杆菌活菌与溶藻弧菌活菌配合使用效果更佳。     

基于CORS网络的单频GPS实时精密单点定位新方法

利用单频接收机实现大范围、高精度快速实时定位是卫星大地测量研究的热点和难点问题之一.本文提出了一套基于连续运行参考站系统(CORS)的误差实时建模数据处理方法,设计并实现了一种新的GPS单频接收机实时精密单点定位服务方法.然后,利用山西省CORS网内一组实测动态数据,以及从湖北省CORS网和武汉市CORS网选取的一组实测静态数据进行测试,验证分析了所提出方法用于实时定位服务的可行性.结果表明:对于站间平均距离小于71 km的参考网,网内单频用户按照实时处理模式,在经过数秒钟的初始化后即可获得厘米级的动态精密单点定位结果.这为GPS单频接收机用户实现快速实时精密单点定位提供了一种可行的新方法.     

The Southern Ontario All-sky Meteor Camera Network

We have developed an automated network of all-sky CCD video systems to detect medium–large meteoroids ablating over Southern Ontario, Canada. The system currently consists of five stations with the largest baseline being 180 km. Each site runs a video rate recorder with sufficient resolution to determine meteoroid trajectories with a typical precision of about 300 m but no worse than 1 km. The sensitivity of the camera is close to a stellar visual magnitude of +1 which allows for astrometric calibrations using field stars. Photometric procedures have also been developed and tested. The system has a limiting magnitude for meteors of about −2 with the current detection algorithm.     

大竹蛏(Solen grandis)翻译控制肿瘤蛋白(TCTP)基因的分子克隆和表达特征分析

本研究克隆得到了一个大竹蛏(Solen grandis)翻译控制肿瘤蛋白(TCTP)基因(SgTCTP)的cDNA全长,其序列全长为1055bp,5′和3′端的非编码区(UTR)分别为54和461bp,开放阅读框(ORF)540bp,编码179个氨基酸,理论等电点为4.50,预测分子量大小19.96kDa。通过荧光定量PCR法检测了SgTCTP在健康大竹蛏各组织中和病原相关分子模式(PAMPs)刺激后的表达规律,结果表明:SgTCTP在检测组织外套膜、鳃、性腺、血细胞、肌肉和肝胰腺中都有表达,其中在肝胰腺中的表达量最高。脂多糖(LPS)、肽聚糖(PGN)和葡聚糖(β-1,3-glucan)刺激都能诱导SgTCTP的表达量上调,SgTCTP的表达量分别在LPS和PGN刺激后6和3h达到最高,为空白对照的3.64和3.36倍;β-1,3-glucan刺激后SgTCTP的表达量上升幅度最大,在12h达到最高,为空白对照的11.76倍。SgTCTP可能作为急性时相蛋白参与大竹蛏的免疫应答。     

Experimental test of asymmetrical cable-stayed bridges using MR-damper for vibration control

In this paper, a semi-active control by MR-damper is researched; its purpose is to effectively control vibration of asymmetrical cable-stayed bridges when earthquake is loaded on the type of bridge. For an experimental study, a model of 10.2 m high and 28 m long asymmetrical cable-stayed bridge structure was built being similar to a real one in size and function. A MR damper was also designed in proper size suitable for the control of the model. The experiment was performed in the way in which three piers were fixed on three shaking tables with 30% of El-centro earthquake wave, and a control device was placed on the lower part of its upper deck for horizontal control. As for control algorithms, Lyapunov and Clipped-optimal control algorithms were applied. The effectiveness of the semi-active control with MR damper for the asymmetrical cable-stayed bridge was measured under five control conditions: Un-control, Passive-off, Passive-on, Lyapunov Control, Clipped-optimal Control. The experiment showed that the semi-active control applying Lyapunov and Clipped-optimal algorithms effectively increased controllability almost in double, and decreased displacement 75% compared with the condition of passive-off. Therefore the semi-active method suggested in this paper is proven effective in controlling asymmetrical cable-stayed bridges.     

X射线极光强度与亚暴指数AE的相关关系

研究了Polar卫星的极区电离层X射线成像仪（PIXIE）得到的极光X射线成像强度AI（Auroral Intensity）与磁层亚暴指数的相关关系.本文发现,在所选取的1997年至2001年的部分数据中,从完整的X射线图像得到的极光X射线总强度和AE指数有很好的线性相关关系,在全部83组数据中有566％的数据的线性相关系数都在060以上（相关系数最大为097）.所以本文认为极光X射线总强度可以作为新的磁层亚暴卫星实时监测指数.     

大黄鱼EBI3基因的克隆与表达分析

通过克隆大黄鱼(Larimichthys crocea)的EBI3(Epstein-Barr virus-induced gene 3)基因并对其进行了序列分析.大黄鱼EBI3(Lyc EBI3)基因开放阅读框长747bp,编码248个氨基酸,存在1个信号肽序列和2个FN3结构域(37~130,145~230).多序列比对发现Lyc EBI3分子与其他已知EBI3氨基酸水平一致性为27.68%~57.53%.Real-time PCR结果表明,Lyc EBI3基因在脾脏和血液中表达量最高,且溶藻弧菌(Vibrio alginolyticus)刺激后,大黄鱼头肾和脾脏中Lyc EBI3基因的转录水平会显著升高,说明Lyc EBI3基因可能参与了抑制由细菌引起的炎症反应.     

基于GNSS网络的实时精密单点定位及精度分析

基于局域GNSS网络,以历元间、星间差分技术实时估计了GPS卫星相对钟差的历元间差值;针对所估计实时精密钟差的特征,推导了实时精密单点定位的估计模型.对所估计得到的相对钟差的历元间差值、实时定位结果分别进行了比较、分析.结果表明,相对钟差的历元间差值与IGS的最终星历相比其精度可以达到0.08 ns;每小时观测的实时静态定位结果在N、E、U三个方向的精度分别为1.47、3.62、4.09 cm.动态模式,实时结果在N、E、U三个方向的精度分别为2.63、3.82、5.20 cm.与采用IGS最终轨道和钟差解算的结果相比较,实时计算结果优于采用精密轨道和精密钟差计算结果.     

Sequential monitoring of beach litter using webcams

This study attempts to establish a system for the sequential monitoring of beach litter using webcams placed at the Ookushi beach, Goto Islands, Japan, to establish the temporal variability in the quantities of beach litter every 90 min over a one and a half year period. The time series of the quantities of beach litter, computed by counting pixels with a greater lightness than a threshold value in photographs, shows that litter does not increase monotonically on the beach, but fluctuates mainly on a monthly time scale or less. To investigate what factors influence this variability, the time derivative of the quantity of beach litter is compared with satellite-derived wind speeds. It is found that the beach litter quantities vary largely with winds, but there may be other influencing factors.     

新一代天气雷达站网实时运行监控系统的设计与开发

新一代天气雷达站网实时监控系统运用了现代计算机网络、工业控制、数据远程传输、远程控制等多项技术,充分利用新一代天气雷达自身系统监控、重要参数定标、检测等功能进行实时监控系统的设计开发,实现了站网内雷达运行状态、定标及报警等相关信息的实时监控,对全面掌握分中心责任范围内各雷达站的运行状况,提高技术保障系统的快速反应能力,以及全面、快速、有效地进行新一代天气雷达系统设备的技术保障具有重要意义。该文主要概述了新一代天气雷达站网实时监控系统的主要功能、系统结构及关键技术的实现方法。