海水网箱养殖石斑鱼病原菌研究

从网箱养殖的石斑Ｅｐｉｎｅｐｈｙｅｌｕｓｓｐ．中分离出一种致病菌——创伤弧菌Ｖｉｂｒｉｏｖｕｌｎｉｆｉｃｕｓ。本文报道该病原菌的分离、形态、毒力、生理生化特性。该菌毒力较强，感染死亡率可达８０％以上。     

光照强度对隐藻吸收铁和磷的影响

在模拟条件下,通过深入研究光照强度对隐藻生长率的影响,以及不同光照强度下藻细胞对Fe和P的吸收差异,提出了光照强度控制藻细胞对营养元素的吸收,最终限制赤潮藻爆发性增殖的作用途径.研究表明,藻细胞的生长率与光照强度之间呈指数相关关系;隐藻生长的光饱和值与光半饱和值分别为150 μmol/(m2·s)和47 μmol/(m2·s);在低光照强度下藻细胞的生长率较低,但是藻细胞却需要吸收更多的Fe和P,以及Zn、Mn、Co和Mo等微量元素,在光照强度为10 μmol/(m2·s)时,藻细胞生长率仅为光照强度150 μmol/(m2·s)时的十分之一,而藻细胞对Fe和P的吸收量分别提高了1.5倍和1倍.这些研究结果为揭示导致赤潮常在光照良好天气后发生的根本原因提供了全新的认识.     

垦利碱蓬Suaeda kenliensis J.W.Xing sp.nov.——碱蓬属一新种

报道了中国碱蓬属植物一新种——垦利碱蓬Suaeda kenliensis J.W.Xing sp.nov.。该种与南方碱蓬Suaeda australis(R.Br.)Moq.相近,但系一年生,种子具圆盘状和卵形两种形状,直径约2～3mm,叶长可达5～7 cm,无不定根,与南方碱蓬相区别。垦利碱蓬生长于潮间带和海水周期性淹没处。耐高盐,区域内海水盐度为25,滩涂表层含盐量在退潮后经日晒蒸发可达60‰以上。常形成单一种群的红海滩植被景观。模式标本存:中国科学院海洋生物标本馆;编号:MBM286537。采集地:莱州湾垦利海域潮间带泥滩。采集人:邢军武、曲宁、钟芳。     

池塘养殖脊尾白虾(Exopalaemon carinicauda)感染血卵涡鞭虫的研究

通过现场取样、显微镜检、细菌培养、组织病理、电镜观察以及PCR检测等研究手段,对2008年9月浙江省舟山市普陀区某梭子蟹养殖塘混养的脊尾白虾"白浊病"进行了系统的病原学研究。结果表明,病虾体色发白,反应迟钝,血淋巴液呈浊白色,严重患病白虾血淋巴中的血细胞几乎没有或者极少,代之以大量与血细胞形似的寄生虫;组织病理切片及电镜观察发现,病虾心脏、肝胰腺、肌肉、鳃部等组织寄生有大量单细胞寄生虫,该寄生虫虫体形态特征、显微结构及宿主临床症状与本实验室之前研究报道的感染三疣梭子蟹和锯缘青蟹的血卵涡鞭虫类似;采用已建立的针对血卵涡鞭虫ITS1基因的引物对"白浊病"病虾的血淋巴样品进行PCR检测,可扩增出585bp左右的特异条带。综合以上研究结果,确定该寄生虫为血卵涡鞭虫(Hematodinium sp.)。有关脊尾白虾感染该寄生虫的报道在国内外尚属首次。     

Biological and physical events involved in the origin,effects, and control of organic matter in solar saltworks

Aspects of communities and events in the concentrating ponds (S.G. 1.130 to 1.214) and salt crystallizing ponds (S.G. 1.215 to 1.264) of solar saltworks pertinent to salt manufacture are described. Communities that aid salt manufacture enable continuous and efficient production of high quality salt at a saltworks' design capacity, and they provide important controls on levels of organic matter in the brine. Fluctuating salinities, high concentrations of nutrients, and petroleum products are disturbances that causeAphanothece halophytica andDunaliella salina to release excessive quantities of organic matter, and that suppress or cause death to nutrient stripping organisms. Disturbances result in decreased quality and quantity of salt and increased costs for salt harvest, washing, and pond upkeep. Organic matter can be controlled by management techniques that keep nutrient stripping communities at proper levels and maintain a narrow and unchanging range of salinities in each pond, by constructing pond dikes able to withstand wind and water erosion, and by preventing spills of petroleum products in the ponds.     

A ‘mathematical’ spider living on gravel plains of the Namib Desert

In certain areas of the Namib gravel plains a new Ariadna sp. was discovered. It rims its vertical burrow with a stone circle, made on average of seven quartz stones. Some mathematical relationships between hole diameter, stone sizes and weight, and animal size were studied. A correlation was found, and stone selection by this spider postulated. Different hypotheses on the adaptive value of stone circle are suggested.     

Fossil nothofagaceous leaves from the Eocene of western Antarctica and their bearing on the origin, dispersal and systematics of Nothofagus

Fossil leaves resembling Nothofagaceae have been investigated from the Eocene of western Antarctica and a new form genus Nothofagofolia is proposed for these kinds of fossils. Some new specimens belonging to this form genus are described. They were collected from the Fossil Hill locality of Fildes Peninsula, King George Island, South Shetland Islands, western Antarctica. Two new species, two new combinations and an unnamed species are reported. A number of published Nothofagus leaf fossils from the same locality are discussed and revised. As a result of these studies of Nothofagus leaf morphology, we conclude that (1) Nothofagus probably originated in high latitudes of the Southern Hemisphere during the middle-late Late Cretaceous and diversified, dispersed gradually to the lower latitudes of the same hemisphere; (2) leaf morphological characters are significant for the systematics of the family Nothofagaceae, especially at the intrageneric level; and (3) extant species of Nothofagus known from southern temperate areas have more primitive leaf morphological characters and lower leaf ranks than those from tropical mountains as well as those of the Fagaceae and Betulaceae. Supported by the Foundation of State Key Laboratory of Palaeobiology & Stratigraphy, Nanjing Institute of Geology and Palaoentology, Chinese Academy of Sciences (Grant No. 013106), the National Natural Science Foundation of China (Grant No. 30670159) and the Fund of Innovation Program by Nanjing Institute of Geology and Palaeontology, Chinese Academy of Sciences     

四川南部珙县早侏罗世一新蜥脚类恐龙

简介在珙县下侏罗统自流井组东岳庙段中,发现的原始蜥脚类恐龙化石的一新属种石碑珙县龙。成年个体长约１４ｍ。化石材料相当丰富,除头骨不完全外,其他部分乃至全部均保存完好。它是早期的原始的蜥脚类,但又和蜥脚类有不少共同点。它对研究蜥脚类的起源和进化,具有重要意义     

High connectivity and directional gene flow in European Atlantic and Mediterranean populations of Ciona intestinalis sp. A

Understanding the factors that cause population divergence has long been of interest to marine biologists in their attempts to interpret the effect of human‐mediated vectors. Broadcast‐spawning species with limited dispersal capability are excellent candidates to measure the present‐day patterns of genetic diversity. The tunicate Ciona intestinalis (Ascidiacea) is comprised of a complex of morphologically cryptic species that form vigorous aggregates in eutrophic habitats (harbors, gulfs and lagoons) where they can compete with the epibenthic community and cause biofouling problems. This study investigated biogeographic variability and migration patterns of C. intestinalis sp. A along Northeast Atlantic and Mediterranean coasts using microsatellite markers. Data presented here on 371 specimens collected from 17 populations reveal high genetic polymorphism, but with a deficit of heterozygote deficiency. Absence of evidence for isolation by distance suggests that the genetic patterns do not reflect the geographic distribution of sampled populations. Substantial gene flow and artificial potential for dispersal boost high levels of within‐population genetic variability and prevent genetic differentiation within and between seas. A predominant eastward migration pattern was revealed by the data set, with very limited opportunity for C. intestinalis sp. A to travel westward. This directional movement indicates that other properties (e.g. habitat quality, genetic traits, mating system, life cycle) may cause adaptive divergence at a large biogeographic scale.     

中国南海一株固氮类芽孢杆菌的筛选和分离鉴定

为了解中国南海海洋自生固氮菌的种类,作者对采集的南海海底淤泥样品进行了固氮微生物的分离、筛选及鉴定。经过土样沸水加热处理,无氮培养基平板初筛后,对分离获得的细菌固氮酶结构基因nif H进行扩增,并对其固氮酶活性进行检测,最终获得一株能够产芽孢的固氮细菌。对该菌株进行生理生化性状测定、16S r DNA序列分析(Gen Bank登录号KJ627376),并基于nif H、16S r DNA系统进化树分析,确定该菌为一株固氮类芽孢菌(Paenibacillus sp.)NH-1。本研究表明固氮类芽孢杆菌在海洋中确有分布,海洋自生固氮菌的多样性远远超出人们之前的认识。