An experiment on induction of triploidy inEriocheir japonicus hepuensis Dai by heat treatment

Temperature treatment to inhibit extrusion of the polar body of the egg was first used on a lower crustacean,Artemia salina (Gross, 1932), and then was used for inducing triploids of amphibian (Frankhauser and Griffiths, 1939), fish (Svardson, 1945), and mammal (Beatly and Fishchberg, 1949). So far, induction of triploidy has been extensively used to obtain sterile or quick-growth individuals in fish (Swarup, 1956; Lincoln, and Scott, 1983 and Thorgaard, 1986) and mollusk (Stanley et al., 1984), but similar work has not been reported on crab, a higher crustancean.     

STUDIES ON IMMUNODIAGNOSIS OF HEPATOPANCREATICPARVO-LIKE VIRUS DISEASES OF PENAEUS ORIENTALISII. RAPID EARLY DIAGNOSIS

This paper describes a rapid procedure for diagnosis of penaeid viral disease using the immune serological method. The SPA (Staphylococcal Protein A) coagglutination test in early diagnosis of penaeid viral disease has yielded satisfactory results.This is the first time SPA is used in China for diagnoses of viral diseases of marine invertebrates, especially penaeid shrimp.The SPA early diagnostic method is characterized by accuracy, rapidity, simplicity and convenience, low cost, high specificity, strong sensitivity and micro-detectability, and easy dissemination and adaptability in prawn farms.     

蜕壳促长素对斑节对虾、刀额新对虾蜕壳和生长的作用

在对虾人工配合饲料中添加蜕（脱）壳促长素，喂养斑节对虾（ＰｅｎａｅｕｓｍｏｎｏｄｏｎＦａｂｒｉｃｉｕｓ）和刀额新对虾（ＭｅｔａｐｅｎａｅｕｓｅｎｓｉｓＤｅＨａａｎ），观察其促进蜕壳、生长的作用。试验证明：饲料中添加不同浓度（０．５‰。、１．０‰、１．５‰）的蜕壳促长素，均不同程度地促进对虾的蜕壳和生长。添加浓度为１．５％。效果最为明显。值得在生产中应用。     

“海参-对虾”混养海水池塘的食物网结构及营养层级研究

The food sources of aquacultured Apostichopus japonicus and the trophic levels of organisms in a sea cucumber(A. japonicus) and prawn(Penaeus japonica) polyculture system in a saltwater pond in Zhuanghe, Liaoning Province were examined using carbon and nitrogen stable isotopes. Across organisms, δ13C ranged from(–25.47±0.20)‰ to(–16.48±0.17)‰(mean±SD), and δ15N ranged from(4.23±0.49)‰ to(12.44±0.09)‰. The δ13C and δ15N contents of A. japonicus, P. japonica and Fenneropenaeus chinensis were comparatively higher than those of other organisms. Values of δ13C and δ15N revealed that P. japonica, Hemigrapsus sanguineus and Neomysis japonica comprised the largest component of the diet of A. japonicus. The mean trophic level of the organisms in this saltwater pond polyculture system was(2.75±0.08). P. japonica, A. japonicus, F. chinensis,Synechogobius hasta and Neomysis japonica were in the 3rd trophic level(2–3); jellyfish, H. sanguineus and zooplankton were in the 2nd trophic level(1–2); and Enteromorpha prolifera, benthic microalgae, periphyton and suspended matter primarily consisting of phytoplankton, bacteria and humus were in the primary trophic level(0–1).     

鳀鱼蛋白酶水解物的营养评价

对鱼蛋白酶水解物EAPH进行评价。EAPH蛋白质含量88．26％，氨基酸比例会理，必需氨基酸占总氨基酸43．61％，并含丰富的水溶性维生素和微量元素。EAPH对动物的生长效果、饲料效率（FE）、蛋白质效率（PER）、净蛋白质效率（NPR）均与对照乳粉蛋白相近；表征其消化吸收利用率的其消化率（TD）、生物价（BV）、蛋白质净利用率（NPU）均略高于对照乳粉蛋白；小麦粉蛋白经EAPH强化后，其蛋白质消化吸收利用率大幅度提高。EAPH为优良蛋白制品。     

碱性成纤维细胞生长因子与胰岛素样生长因子Ⅱ对中国对虾淋巴细胞培养物的协同诱导作用

对虾的传染性疾病是限制对虾养殖业发展的主要因素.目前至少发现了17种对虾病毒病并鉴定出了30多种对虾病毒[1～5].虽然已在其宿主动物---虾中对对虾病毒病进行了多方面的研究,但为了分离、纯化和鉴定这些病毒,必须建立一个适宜于对虾组织的体外培养系统.繁殖并弄清病毒的分子结构及其理化特性是研究病毒病、检测和治疗病毒病的重要手段,而发展对虾细胞的体外培养和建系技术是解决这些问题的关键之一.     

Sea water environment copper requirement of egg hatching and naupliar metamorphosis of Peaneus chinensis

ＳｅａｗａｔｅｒｅｎｖｉｒｏｎｍｅｎｔｃｏｐｐｅｒｒｅｑｕｉｒｅｍｅｎｔｏｆｅｇｇｈａｔｃｈｉｎｇａｎｄｎａｕｐｌｉａｒｍｅｔａｍｏｒｐｈｏｓｉｓｏｆＰｅａｎｅｕｓｃｈｉｎｅｎｓｉｓ￥ＹｕａｎＹｏｕｘｉａｎ；ＱｕＫｅｍｉｎｇ；ＬｉｕＬｉｂｏ；ＧａｏＣ...     

朱氏扁藻作为中国对虾幼体饵料的效果试验

本文报道了从我国海州湾沿岸海水中分离的一种易培养朱氏扁藻(Tetraselmis chui),此藻投喂中国对虾(Penaeus chinensis)幼苗,成活率可达64.30％(蚤状Ⅰ期到仔虾Ⅰ龄),与对照组同。     

Application of RAPD technology for identification in three different stocks of Penaeus chinensis

INTRODUCTIONPenaeuschinensishasbecomeoneoftheprimaryspeciesforaquacultureinChina .How ever ,viralandbacterialdiseaseproblemshaveplaguedtheshrimpaquacultureindustryserious lyinChinasince 1 993.Ithasbeenindicatedthatthereducedgrowthperformanceandthein creasedsusceptibilitytodiseasescouldbearesultoflowlevelsofgeneticvariablities (Carretal .,1 994 ) .InordertosupporttheaquacultureindustryoftheUnitedStates,theUSMarineShrimpFarmaringProgramConsortiumhasdevelopedvariousdisease free populati…