几种优良养殖对虾杂合性的比较研究

应用聚丙烯酰胺凝胶电泳技术研究了南美白对虾（Penaeus vannamei)，日本对虾（Penaeus japonicus)，斑节对虾（Penaeus monodon)，新对虾（Metapenaeus ensis)养殖群体杂合体缺乏及过量。结果表明南美白对虾在Est-2(F=0.705),Aat-2(F=1.000)，两个位点上存在杂合子缺乏，在Adh-3(F=-1.000),Sdh-2(F=-1.000),Mdh-4(F=-1.000)三个位点上存在杂合子过量；日本对虾在Est-1(F=0.258),Sod-1(F=0.467)两个位点上存在杂合子缺乏；斑节对虾在Est-1(F=0.254),Sdh-3(F=1.000)两个位点上存在杂合子缺乏；新对虾在Aat-1(F=1.000)位点上存在杂合子缺乏，在Est-1(F=-0.158),Sod-1(F=-1.000),Me-4(-F=1.000)三个位点上存在杂合子过量。导致杂合子缺乏的主要原因可能是自然选择，近交，Wahland效应等。杂合子缺乏会导致某些基因从基因库中消失，造成种群遗传多样性的降低，从而降低物种适应环境的能力。     

凡纳滨对虾高密度养殖实验

实验时间 4 0天 ,放苗密度 5 0 0尾 /m2 、10 0 0尾 /m2 。凡纳滨对虾初体长为 2 .5 88±0 .341cm ,初体重 (0 .2 39± 0 .0 86 ) g。养殖水体中放置筛绢隔断 ,在不换水的条件下 ,监测水体中无机磷、氨氮的变化趋势 ,初步实验高密度和分隔养殖的效果。结果表明 ,高、低密度氨氮、无机磷差异均为极显著 (P <0 .0 1) ;成活率、产量差异均为显著 (P <0 .0 5 )。高、低密度特定生长率 体重 差异不显著 (P >0 .0 5 )。观察到不同时间不同密度凡纳滨对虾对筛绢隔断的反应不同 ,表明对虾能利用隔断筛绢 ,从而有效提高养殖容量     

The DNA de-methylating agent 5-azacytidine does not restore CYP1A induction in PCB resistant Newark Bay killifish (Fundulus heteroclitus)

Newark Bay (NB) killifish (Fundulus heteroclitus) have been chronically exposed to environmental contaminants that activate the aryl hydrocarbon receptor (AHR) and are tolerant to toxic effects and CYP1A induction provoked by AHR ligands. Resistance to CYP1A induction could be due to an epigenetic mechanism such as DNA methylation. We measured in-ovo CYP1A catalytic activity (ethoxyresorufin-O-deethylase, EROD) in NB and reference site killifish embryos aqueously exposed to various concentrations of the de-methylating agent 5-azacytidine, 5-AC (5, 50 and 500 μ(micro)M) with or without 0.2 μ(micro)g/l of the CYP1A inducer 3,3^′,4,4^′,5 pentachlorobiphenyl (IUPAC PCB126). Neither PCB126 alone, nor PCB126 plus 5-AC, induced EROD above levels in vehicle treated Newark Bay fish. In reference site fish, the same PCB126 dose provoked a 7.4-fold EROD induction relative to controls. We conclude that Newark Bay killifish are resistant to CYP1A induction by co-planar PCBs during early embryological development and our data suggests that DNA methylation does not play a critical role in resistance to CYP1A induction in this model.     

中国对虾配合饲料限制性营养成分分析

以最常用的18种原料为对象,用线性规划的方法对华东沿海中国对虾人工饲料配制过程中的限制营养成分进行了分析。结果表明,粗脂肪、粗灰分、磷、精氨酸、赖氨酸是限制性营养成分,粗纤维和蛋氨酸在以植物性蛋白质为主的配方中是限制性营养成分。因此,在中国对虾人工饵料配方设计中,应注意选用脱脂原料,增加矿物质添加剂中磷的含量以及采用富含精氨酸和赖氨酸的原料。     

Seasonal use of coastal resources by otters: Comparing sandy and rocky stretches

Seasonal patterns of use of coastal resources by otters have been studied, simultaneously performing diet analyses and spraint counts (expressed as marking intensity, MI). In spite of the small size of the study area, two different sectors could be clearly identified, a sandy and a rocky one. The origin (freshwater or coastal) of most prey types could also be determined. Surveys were performed bimonthly and classified as autumn–winter (A–W) or spring–summer (S–S).In the sandy sector, eels, flatfish and grey mullets were more frequently consumed in A–W, while crayfish was more frequently consumed in S–S. None of the identified prey types in the rocky sectors showed different frequency of occurrence values between the two periods. In this sector otter fed mainly on coastal prey throughout the year, while in the sandy one there was a clear shift from coastal prey to freshwater ones in S–S. The decrease of coastal prey consumption in S–S in the sandy sector coincided with the lowest MI values, showing that in this period otters use coastal areas less intensely. Though shifts in otter diet and use of space in the sandy sector are probably influenced by the population dynamics of estuarine fish species and crayfish, the increased tourist disturbance during spring and summer in the sandy sector could be an important determinant of the observed patterns. Finally, the results show that MI can be used as a reliable measure of habitat use by otters, at least in small areas.     

低盐度地下卤水养殖南美白对虾的研究

研究了用低盐度地下卤水养殖南美白对虾（Penaeus vannamei)的可行性和养殖模式。结果表明，向地下卤水中添加适量的K^ ，调节Na^ /K^ 比值，能明显提高仔虾的成活率；通过合理的水质调控，获得低盐度地下餐水南美白对虾养殖的高产高效，其中精养池平均成活率，平均产量和投入产出分别比半精养提高25．23％，148g/m^2和0．49。     

中国对虾配合饵料中维生素C添加量的研究

于１９９１年７月２１日－８月１９日在河北省唐海县西林养殖场以养成期中国对虾为材料，在其配合饵料中添加维生素Ｃ进行中国对虾对维生素Ｃ需要量的试验。结果表明，当中国对虾体长为５．５－７．５ｃｍ时，若以本项研究采用的配合饵料的基本成分和配比为基础，配饵中维生素Ｃ的最佳变化添加量为０．０１８％－０．０３０％。研究发现，变动的维生素Ｃ适宜添加量对于中国对虾具有明显的促进生长，增强抗病力和抗低氧能力以及提高存     

Study on genetic diversity of cultivated populations in 4 species of shrimps

Genetic diversity of cultivated populations was investigated using assay of vertical slabpolyacrylamide gel electrophoresis in 4 species of shrimps, which were Penaeus japonicus, Penaeus monodon , Penaeus vannamei and Metapenaeus ensis, The results showed that the mean number of alle-les per locus (A) is 1.3±0.2, 1.3±0.1, 1.3±0.1 and 1.3 ±0.1 respectively; the percentage of polymorphic loci (P0.95) is 12.5, 6.7, 20 and 23.5 respectively; the expected heterozygosity (He) is 0.042±0.034,0.042±0.031, 0.094±0.042 and 0.097±0.047 respectively; and the observed heterozygosity (H0)is 0.029±0.024, 0.028±0.023,0.154±0.082 and 0.150±0.084 respectively. The difference of genetic diversity is obvious in 4 species of shrimps. The degree of genetic diversity is M. ensis > P. vannamei > P. japonicus > P. monodon. In short, the lower level of genetic diversity is estimated in 4 species of shrimps.