Assessing cause and effect of multiple stressors on marine systems

An operational framework is developed to serve as a guideline for investigating causal relationships between environmental stressors and effects on marine biota. Because of the complexity and variability of many marine systems, multiple lines of evidence are needed to understand relationships between stressors and effects on marine resources. Within this framework, a weight of evidence approach based on multiple lines of evidence are developed and applied in a sequential manner by (1) characterizing the study system which involves determining if target biota are impaired, assessment of food and habitat availability, and measuring contaminant levels in the environment, (2) assessing direct effects of contaminant exposure on target biota using biomarkers and assessing indirect effects of exposure using suites of bioindicators, and (3) applying standard causal criteria based on epidemiological principles and diagnostic health profiling techniques to assess potential causes. Use of multiple lines of evidence should also reduce the risk of false positives (Type I error or falsely concluding that there is a causal relationship when there is none) and false negatives (Type II error or falsely concluding there is not a causal relationship when there actually is). Understanding causal relationships and the mechanistic processes between environmental stressors and effects on biota is important in the effective management and restoration of impaired marine ecosystems.     

NCEP/CFS模式对东亚夏季延伸预报的检验评估

利用NCEP的气候预报系统（Climate Forecast System, CFS）所提供的1981—2004年历史回报试验结果,检验和评估了该系统对夏季东亚地区大气环流的预报技巧和系统误差;在此基础上通过提取模式预报和观测的10~20 d及30~60 d低频振荡分量,重点对我国南方3次典型持续性暴雨过程的预报技巧进行检验和诊断分析。结果表明:CFS系统对东亚整体大气环流逐日预报的可靠时效为5 d左右,60°N以北的对流层中高层高度场预报系统性偏低,而在40°~60°N则为系统性偏高。系统性误差随预报时间的延长而增加,但10 d以上预报的系统性误差大小和空间分布逐渐趋于稳定;CFS系统对低频分量的延伸期预报技巧好于对其整体大气环流的预报技巧,并且在典型持续性暴雨过程中,CFS系统对影响强降水过程的主要环流系统低频振荡特征有一定预报能力。     

Possible Sources of Forecast Errors Generated by the Global/Regional Assimilation and Prediction System for Landfalling Tropical Cyclones. Part Ⅱ: Model Uncertainty

This paper investigates the possible sources of errors associated with tropical cyclone (TC) tracks forecasted using the Global/Regional Assimilation and Prediction System (GRAPES). In Part I, it is shown that the model error of GRAPES may be the main cause of poor forecasts of landfalling TCs. Thus, a further examination of the model error is the focus of Part II. Considering model error as a type of forcing, the model error can be represented by the combination of good forecasts and bad forecasts. Results show that there are systematic model errors. The model error of the geopotential height component has periodic features, with a period of 24 h and a global pattern of wavenumber 2 from west to east located between 60°S and 60°N. This periodic model error presents similar features as the atmospheric semidiurnal tide, which reflect signals from tropical diabatic heating, indicating that the parameter errors related to the tropical diabatic heating may be the source of the periodic model error. The above model errors are subtracted from the forecast equation and a series of new forecasts are made. The average forecasting capability using the rectified model is improved compared to simply improving the initial conditions of the original GRAPES model. This confirms the strong impact of the periodic model error on landfalling TC track forecasts. Besides, if the model error used to rectify the model is obtained from an examination of additional TCs, the forecasting capabilities of the corresponding rectified model will be improved.     

BCC S2S模式对亚洲夏季风准双周振荡预报评估

利用1994-2013年ERA-Interim及NCEP/NCAR再分析数据,对国家气候中心（BCC）次季节到季节尺度模式（S2S）1994-2013年的回报试验数据进行亚洲季风区准双周振荡（QBWO）预报能力评估,并诊断模式预报误差来源。结果表明:BCC S2S模式对QBWO的预报能力随着预报提前时间的增长而降低,9 d后预报技巧明显减弱,其周期、传播特征和强度出现误差;在提前9 d预报中,印度洋地区QBWO对流-环流系统结构松散,信号偏弱,对流向东传播,这与印度洋平均态的预报误差有关,夏季对流平均态低层水汽场在西太平洋和阿拉伯海较强,而东印度洋、孟加拉湾一带偏弱;西北太平洋地区QBWO具有向西北传播的特征,但强度偏弱,可能原因是预报低估了QBWO对流西北侧低层涡度的超前信号,经涡度方程诊断发现,地转涡度平流正贡献微弱,相对涡度平流在对流西北侧引发负涡度,从而减弱了对流西北侧由低层正涡度引发的有利条件。     

CINRAD/SA(B)发射机触发器芯片级故障诊断流程

发射机触发放大器主要为调制器中SCR开关管提供放电触发脉冲,同时兼具调制组件的保护功能。发射机触发器芯片级故障诊断流程将触发器故障定位到芯片级,大大降低了触发器故障维修成本,同时为雷达测试平台中触发器故障自动诊断建模提供了技术支持。在发射机触发器信号流程基础上,依据发射机触发器关键点波形或关键点电平,通过触发器故障树图了解故障因果关系,研究出规范化的触发器芯片级故障诊断流程。列举了依据触发器芯片级故障诊断流程,修复了由于单稳态触发器无触发信号输出,导致无-200V放电触发信号的单稳态触发器故障个例。结果表明:芯片级触发器故障诊断流程可以快速定位发射机触发器故障点到最小可更换芯片。具有操作方法简洁、诊断思路清晰、操作规范,基层雷达站技术人员容易掌握的特点,可有效提高新一代天气雷达技术保障水平,保证雷达观测数据的可靠性。     

A Sensitive and Versatile Multiplex PCR System for the Rapid Detection of Enterotoxigenic (ETEC), Enterohaemorrhagic (EHEC) and Enteropathogenic (EPEC) Strains of Escherichia coli

Although Escherichia coli is widely distributed in the marine environment, only a small percentage are pathogenic to humans. Nonetheless, the widespread occurrence of waterborne infections of E. coli origin in humans has become one of the major health problems worldwide. To date, several types of enterovirulent E. coli have been recognized as the aetiologic agents of various gastrointestinal infections in humans. The most commonly encountered are those belonging to the enterotoxigenic (ETEC), enteroinvasive (EIEC), enterohaemorrhagic (EHEC) and enteropathogenic (EPEC) subtypes. In order to better determine the health risks that are associated with exposure to some of these specific subtypes, we have developed a very sensitive multiplex PCR system for the rapid detection and typing of ETEC, EHEC and possibly EPEC strains of E. coli in the aquatic environment. The target genes chosen for this investigation included: the PHO-A housekeeping gene (present in all E. coli); the LT1, LT2 and ST1 genes of ETEC; the VT1 and VT2 verotoxin, and EAE virulence genes of EHEC and EPEC, respectively. Six pairs of oligonucleotide primers were designed to simultaneously amplify internal fragments of these genes by multiplex PCR to generate PCR products that could be analysed and confirmed with relative ease by gel electrophoresis and HincII enzyme digestion. The results showed that the six sets of PCR primers were highly specific for their target genes and produced specific amplimers of the expected size from several control strains of E. coli – ATCC 35401 (LT1+/ST1+); SA53 (LT2+/VT2+); and O157 (VT1+/VT2+/EAE+). The detection sensitivity of the multiplex PCR system for the six target genes in an E. coli cell mixture was optimized and enhanced by preincubating serially diluted cells in Luria-Bertani broth for 6 h prior to PCR analysis. The results obtained indicated a detection sensitivity of 10° CFU (of each strain) per 100 μl reaction. Multiplex PCR analysis of seawater samples collected from four sewage-polluted sites in Hong Kong indicated the presence in all four samples of E. coli bacteria that were positive for LT1, ST1, VT1 and EAE virulence genes. Overall, the data indicated that the multiplex PCR system described in this study is a potentially very useful and powerful method for routine monitoring and risk assessment of water quality.     

NMC第二代中期数值预报系统误差的动力诊断

利用国家气象中心第二代中期数值预报模式计算了１月和７月的预报诊断量。计算结果表明,新一代模式在动力外强迫作用、辐射通量和对流参数化等物理过程方面的改进,使该模式的预报比第一代模式有了较大的改善,模式预报的系统误差明显减小。通过对角动量、热量和水汽的经向输送和收支平衡的分析,认为该模式在地形处理、陆面过程、云辐射和积云对流参数化等物理过程还有待进一步改进。     

几个方兴未艾的气候学问题

气候学是大气科学中发展最快的一个分支．文中重点总结了几个有关气候诊断与气候预测问题的研究进展,包括大气环流基本模态、夏季风与ＥＮＳＯ、ＥＮＳＯ预测、季度预测及年代际气候变率     

IAP T42L9全球谱模式加热场的诊断

就一次出梅时副高北进过程, 对IAP T42L9全球谱模式加热场与利用ECMWF同化系统资料根据热力学方程反算的加热场进行了比较。结果表明:模式参数化加热场的水平分布基本合理,但低纬和南半球中高纬地区加热偏弱; 参数化加热的垂直分布在北半球比较合理,但在南半球与反算法的结果有较大差别。这些结果对模式能很好地预报北半球中高纬环流形势演变,较好地预报西太平洋副高变化及预报的副高系统性偏弱给出了较好的解释。     

以哈维氏弧菌(Vibrio harveyi)为重点的鱼类细菌病原的诊断与控制(英文)

作者对鱼类细菌病原的诊断与控制作了较全面的综述。细菌病原诊断技术包括酶联免疫吸附技术（ＥＬＩＳＡ）及蛋白印迹法（Ｗｅｓｔｅｒｎｂｌｏｔｉｎｇ）。细菌病原控制技术包括化学疗剂的应用,有益微生物的应用,营养添加剂,β－葡萄糖苷增强免疫抗病力,及免疫保护作用等。