Correlation of CYP1A1 induction, as measured by the P450 RGS biomarker assay, with high molecular weight PAHs in mussels deployed at various sites in San Diego Bay in 1993 and 1995

In 1993 collections of marine mussels (Mytilus galloprovincialis) were deployed 1 m from the water surface at six sites in San Diego Bay for 88 days. A similar mussel deployment was conducted in 1995, except the animals were deployed 1 m off the bottom and only for 32 days. After recovery from the sites, tissue was extracted with dichloromethane and the solvent extracts analyzed for chemical contaminant content and the ability to produce CYP1A1 induction in a transgenic cell line (TV101L cells). The cells used in the assay (P450 RGS) are stably transfected with a plasmid containing firefly luciferase linked to human CYP1A1 promoter sequences. Induction (fold increase compared to control) was determined by luminometry 16 h after application of small volumes (2–10 μl) of solvent extracts to cultured cells. Small mussels deployed in the Naval Station (NAV) in 1993 exhibited very high bioaccumulation of polycyclic aromatic hydrocarbons (PAHs; 52 μg/g) and polychlorinated biphenyls (PCBs), in addition to very strong induction of CYP1A1 measured by reporter gene system (RGS) responses. Large mussels deployed at the NAV station in 1993 and intermediate-sized animals placed at three stations within the NAV station in 1995 accumulated 13–29 μg PAH/g and exhibited relatively high RGS responses. Correlation of RGS responses for all mussel samples to the measured PAH concentrations was 0.85 (r²). When the concentrations of seven specific PAHs found in the samples are converted to benzo[a]pyrene equivalents, from previously derived toxic equivalency factors (TEFs) for this test system, and compared to measured RGS responses, the correlations are approximately 0.9. The results of these studies indicate that the RGS biomarker can be used as a screening tool for detection of CYP1A1-inducing compounds in tissues, and an estimate of potential human health or ecological risk from ingestion of contaminated organisms. Positive RGS responses can be followed by detailed chemical analyses of PAHs and coplanar PCBs using the same extract.     

川滇地区三维P波速度结构反演与构造分析

根据205个区域台站记录的近60000条地震初至P波走时资料, 采用层析成像理论与伪弯曲射线追踪方法, 反演了川滇地区地壳上地幔的三维P波速度结构.结合区域地质构造以及地球物理背景, 分析和解释了三维速度结构图像反映的川滇地区不同深度的介质结构与构造特征.结果表明: (1) 沉积盆地、高山山地等主要表现出速度负异常的特征, 有的高山山体负异常可深达下地壳与上地幔, 反映了新造山带的强烈构造隆升与相伴的重力均衡作用; (2) 川滇块体周缘大型活动断裂带附近的中下地壳内普遍存在低速层, 它们的存在为调节断裂和块体运动提供了深部解耦条件; (3) 根据对P波速度结构图像的分析, 识别和推断出川滇地区若干主要活动断裂的深部构造特征及它们倾向与下延深度.      

兰州盆地黄河800ka B.P.阶地的发现及其古地磁年代

兰州东盆地的枣树沟黄河第四级阶地(T4),砾石层顶高出黄河河面84 m,其上堆积厚约64 m的风成黄土,黄土地层中包括八层古土壤,最底部发育了古土壤S8.光释光(OSL)测年结果显示古土壤S1上部的年龄为70.4±7.6 ka.黄土古土壤对比以及磁性地层学研究表明,枣树沟T4上覆风成黄土的年龄为865 ka,T4至少形成于865 ka B.P..     

近40ka来海南岛海岸沙地气候与环境演变

选择海南岛东、西海岸具有代表性和高分辨率特征的木堆、棋子湾等地层剖面,在建立年代序列的基础上,对风成沙粒度组成与参数、SC/D值及磁化率等代用指标的研究表明,末次冰期间冰阶时气候相对温暖,滨海平原上河流三角洲前移,湿地、沙丘与交错分布;末次盛冰期时气候干凉,海岸沙地上形成了多道平行延伸的古沙垄(丘),并与大陆架古沙丘形成连续性沉积;末次冰消期时气候快速变化,古沙垄(丘)或加积发育或固定成壤;全新世以来气候回暖并波动变化,次生沙丘经历多次半固定、固定－沙丘加积、活化的演变。     

Benthic input rates predict seagrass (Posidonia oceanica) fish farm-induced decline

Fish farms represent a growing source of anthropogenic disturbance to benthic communities, and efficient predictors of such impacts are urgently needed. We explored the effects of fish farm benthic organic and nutrient inputs on the population dynamics of a key seagrass species (Posidonia oceanica) in four Mediterranean deep meadows adjacent to sea bream and sea bass farms. We performed two annual plant censuses on permanent plots at increasing distance from farms and measured benthic sedimentation rates around plots. High shoot mortality rates were recorded near the cages, up to 20 times greater than at control sites. Recruitment rates increased in variability but could not compensate mortality, leading to rapid seagrass decline within the first 100 m from cages. Seagrass mortality increased with total sedimentation rates (K=0.55, p<0.0002), and with organic matter (K=0.50, p=0.001), total nitrogen (K=0.46, p=0.002) and total phosphorus (K=0.56, p<3.10(-5)) inputs. P. oceanica decline accelerated above a phosphorus loading threshold of 50mg m(-2)day(-1). Phosphorus benthic sedimentation rate seems a powerful predictor of seagrass mortality from fish farming. Coupling direct measurements of benthic sedimentation rates with dynamics of key benthic species is proposed as an efficient strategy to predict fish farm impacts to benthic communities.     

华北北部Ms≥5．0地震前震源区附近中小地震主压应力轴的变化特征

通过对华北北部11次Ms≥5.0地震前2年至震后1年震源区(△≤150km)附近中小地震主压应力轴相对背景场的变化研究结果表明,Ms≥5.0地震前9～1个月左右,震源区附近中小地震主压应力轴往往出现45°～90°左右的转向变化,或主压应力轴出现仰角增大,且增大幅度大于45°的异常变化,这种变化一般在震后几个月至1年左右结束。