Stage-V copepodites of Calanus sinicus and Calanus jashnovi (Copepoda: Calanoida) in mesopelagic zone of Sagami Bay as identified with genetic markers, with special reference to their vertical distribution

We applied genetic makers to identify Calanus species occurring in Sagami Bay, Japan, in order to investigate their vertical distribution in the upper 1000 m. First, interspecific genetic distances of three gene loci, mitochondrial small ribosomal RNA (srRNA), nuclear internal transcribed spacers 1 (ITS1) and 2 (ITS2), were estimated from morphologically distinguishable adult females of Calanus sinicus, Calanus jashnovi and Calanus pacificus that were collected from Sagami Bay, the Kuroshio Extension and the Oyashio region, respectively. The highest levels of interspecific genetic distance were observed in srRNA, followed by ITS1 and ITS2. The intraspecific genetic distances within C. sinicus were much lower than the interspecific genetic distances, indicating that DNA sequences in these loci are consistent with the morphological differences. This information was used as a criterion for species identification based on DNA sequence variation, and allowed us to identify the fifth copepodites (CVs) or younger stages of these species. Next, the vertical distribution of Calanus species was investigated in Sagami Bay in May 2006, on the basis of a stratified sampling in the upper 1000 m. By applying the genetic markers, 23 individuals comprising all copepodite stages were allocated into either C. sinicus or C. jashnovi, and the small- and large-sized CVs were identified as C. sinicus and C. jashnovi, respectively. The total abundance of C. sinicus was highest at 0-50 m and decreased with depth. On the contrary, CV individuals of C. sinicus were abundant not only in 0-50 m but also below 200 m with minimum occurrences in 150-200 m depth. C. jashnovi was much less abundant than C. sinicus and comprised of only CIV and CV which occurred in the upper 100 m and deeper than 50 m depths, respectively. The abundance of C. sinicus in the 1000-m water column of Sagami Bay was at a level comparable to that in shelf waters, suggesting the importance of off-shelf individuals in the biological production and organic transport in the respective areas.     

南极普里兹湾NP93－2柱样磁组构特征及其古气候意义

对采自东南极普里兹湾海区沉积物ＮＰ９３－２柱样系统的磁组构分析,结果表明：１．普里兹湾海洋沉积物具有较大的磁化率各向异性,较好地指示了沉积过程中水动力过程的变化;２．由磁化率各向异性主轴方向确定的古水流方面主要为近ＥＷ向及ＮＳ向,分别对应着南极绕极环流的方向及由融冰水的大量生成而导致的南极底层水形成的方向,这两种不同方向的水流在该海区不同时段的表现各不相同;３．依据表征磁组构的参数及古水流方向的变化,可将１２７００ａＢ．Ｐ．以来的南极地区的古气候分成５个古气候变化带．     

运用因子分析评价山东黑泥湾沉积物重金属污染来源

在对山东黑泥湾沉积物中Cu、Pb、Zn、Cd、Hg、As、Cr和有机质进行分析测试的基础上,运用因子分析评价沉积物中重金属污染来源,结果表明,山东黑泥湾沉积物中存在Pb、Zn和Cd污染;主成分分析中前3个主成分的贡献率分别为44.656%、19.068%和18.098%;认为山东黑泥湾重金属污染主要有3个来源:有机质降解、海上船舶排污和环境背景。     

基于ENVI 的唐山湾三岛土地利用遥感分类方法的比较分析

对唐山湾三岛2010年10月10 m分辨率的SPOT5多光谱数据源,采用不同分类方法进行识别和判断,并对比不同分类器在遥感影像分类中的应用效果和分类精度。有针对性地探究海岛土地利用遥感分类过程中不同方法的优劣,获取最适于岛陆地区土地利用的遥感分类方法。根据土地利用现状分类标准(GB/T21010-2007)和海岛陆域土地利用类型划分的相关规定,将唐山湾三岛主要分为滩涂、裸地、林地、草地、居住区、内陆水体和潮间带(潮水覆盖区)6类。并分别构建结合人为控制的非监督分类、监督分类和基于专家知识的决策树分类系统,参照更高分辨率影像、先验知识和野外调查资料,评价分类结果与实地调查结果的吻合程度,最终通过总体分类精度和Kappa系数等指标对各分类器精度进行评价和对比分析。     

GA-BP神经网络在罗源湾口波浪模拟研究中的应用

由于BP神经网络存在收敛速度慢和易于陷入极小值等缺点,引入遗传算法(GA)对网络的权值和阈值加以优化,并采用不同组合的输入因子和样本数,对福建省罗源湾口的波浪进行模拟研究.对输入因子的分析结果表明,研究区域的波浪主要受台湾海峡波浪传播影响,与局地气象因子(风速、风向、海气温差)的相关性较弱.训练样本试验表明,30 d以上的波浪历史数据可使GA-BP神经网络充分学习研究区域的波浪特征,从而实现对波浪要素的高精度模拟.模拟结果显示,对春、夏季实测波浪数据的模拟效果均很好,其中相关性分别为0.967和0.938,均方根误差分别为0.112 m和0.107 m,表明GA-BP神经网络在近岸波浪模拟预报中有较广阔的应用前景.     

渤海湾潮下带底栖线虫群落的垂直分布

2007年春季在渤海湾天津潮下带采集小型底栖生物样品,分析沉积物中自由生活线虫的垂直分布情况。多元统计分析结果表明,底栖线虫的垂直分布和沉积物深度有关。在较深的5～10cm层,线虫群落与其上层次的差异较大:0～2cm层和5～10cm层差异极显著(P=0.001),2～5cm层和5～10cm层间差异显著(P=0.016)。不同站点间底层的线虫群落差异明显。而沉积物0～2cm层和2～5cm间的线虫群落差异不明显(P=0.541)。列出了SIMPER分析中16种明显影响表层-底层群落的线虫,13种明显影响中层-底层间群落比较的线虫。在研究和比较线虫群落时,应该考虑取样深度的影响。     

海州湾近岸张网渔获物种类组成和资源利用现状分析

根据2011年3月~2012年12月海州湾沿岸定置张网调查资料,对海州湾张网渔获物组成进行比较分析。结果表明,张网渔获物组成具有明显的季节变化特征,春季以尖海龙(Syngnathus acus)、六丝钝尾虾虎鱼(Amblychaeturichthys hexanema)、双斑蟳(Charybdis bimaculata)、长蛸(Octopus variabilis)、方氏云鳚(Enedrias fangi)和口虾蛄(Oratosquilla oratoria)为主,秋季主要为小黄鱼(Larimichthys polyactis)、六丝钝尾虾虎鱼、三疣梭子蟹(Portunus trituberculatus)、日本枪乌贼(Loligo japonica)、鹰爪虾(Trachypenaeus curvirostris),冬季主要为六丝钝尾虾虎鱼、口虾蛄、鲜明鼓虾(Alpheus heterocarpus)、细鳌虾(Leptochela gracilis)和日本鼓虾(Alpheus Japonicus)。主要经济鱼类小黄鱼在9、10月产量最高,但达到可捕体长的小黄鱼不足1%,主要经济虾类鹰爪虾在9、10月产量最高,取样中69.46%的鹰爪虾达到可捕体长。渔捞日志记录表明,9月份单船平均网产达到最大值(17.01kg/net),12月份单船平均网产最低(2.87kg/net)。建议对张网渔业加强管理,开展生态型渔具渔法的研究,放大网目尺寸,加强对近海资源的养护。     

Biometric,microstructural, and high-resolution trace element studies in Crassostrea gigas of Cantabria (Bay of Biscay,Spain): Anthropogenic and seasonal influences

Living Crassostrea gigas oysters of different ages and sizes were collected in three estuaries of Cantabria (Bay of Biscay, Spain): San Vicente de la Barquera Estuary, Santander Bay, and Marismas de Santoña Estuary. The main objective was to determine different shell responses to variable environmental parameters. A shell morphological study, based on three biometric indices, indicates that oysters of Santander Bay have two significant shell anomalies: abnormal thickening of the right valve and loss of vital cavity volume. These shell abnormalities are related with the presence in these waters of the chemical tributyltin. In the other two estuaries, the oysters show no detectable anomalies. Four shell microstructures have been distinguished: Regular Simple Prismatic, Regular Foliated, cone-Complex Cross Foliated, and Chalk. In Santander Bay oysters, the Chalk forms a “root-type” framework, whereas in the other two estuaries it forms a more compact microstructure. It is proposed that exposure to tributyltin has produced this modification. High-spatial-resolution geochemical transects have been carried out on the Regular Foliated microstructure in the umbo region in order to evaluate the distribution of Mg, Sr, and Na. The elements analysed exhibit clear cyclic variations in San Vicente de la Barquera Estuary and Marismas de Santoña Estuary oysters, related with seasonal periods, and characterised by broad maxima during months in which the waters are warmer and have higher salinity. These patterns are buffered in Santander Bay oysters. Our results demonstrate that biometric, microstructural, and high-resolution trace element studies in oyster shells can provide information about contaminants and seasonal variations in the estuarine environment.     

渤海湾水生态环境现状和海岸带开发对其的影响研究

由于沿海地区经济迅速发展,越来越多的陆源污水排入渤海湾,渤海湾生态环境系统承受着巨大的压力。本文建立了渤海湾生态环境的多级指标体系,并根据2003年至2005年多次渤海湾监测资料对其现状进行了分析;然后进一步研究了由于围海造地、海水淡化等开发工程引起的近岸海域水动力条件和污染物输移的变化。结果表明,陆源污染物大量排放、沿海开发活动加剧是影响渤海湾水生态环境的主要原因。沿海围垦不仅导致潮通量减少,潮流作用减弱,而且影响近岸海域污染物的输移和分布。海水淡化高浓度盐水的排放也对近岸海域的水生态环境造成很大的影响。     

福建深沪湾潮间带全新世有孔虫及其环境意义

本文对深沪湾西部潮间带表层沉积物和两个钻孔岩芯共30个样品的有孔虫组成进行了定性和定量分析。结果表明，表层沉积物中有孔虫群分布规律明显，随着离岸向海越远，水深越大，有孔虫娄量从1倍数上升到5位数，种数为9-69种，多变度V31-44，复合分异度H（s)3.25-3.25。从钻孔岩芯中的全新世有孔虫群特征可看出，中全新世以来海面升降和海洋环境可分为2个阶段：（1）海水初到钻孔位置，有孔虫数量和种数较少，分异度不高，海相性程度较低。（2）后期（埋深1.8m和2.0m以浅），海面有所升高，变为潮间带（高潮位→中潮位→低潮位）环境。