两株琼胶酶高产细菌的筛选和鉴定

从海洋环境中筛选得到两株琼胶酶的高产菌株，通过形态观察和生理生化反应，确定它们属于弧菌属，通过BIOLOG细菌鉴定系统鉴定，并同弧菌属标准菌株分析比较，确定上方宝剑两株菌都是塔式弧菌（Vibrio tubiashii)，这两株菌在碳源利用方面存在差别。     

The morphodynamic modelling of tidal sand waves on the shoreface

An artificial sand wave on the Dutch shoreface of the North Sea has been studied in conditions with relatively strong tidal currents in the range of 0.5 to 1 m/s and sediments in the medium sand size range of 0.2 to 0.5 mm. The sand wave is perpendicular to the tidal current and has a maximum height and length of the order of 5 m and 1 km, respectively. The sand wave is dynamically active and shows migration rates of the order of a few metres per year. A numerical morphodynamic model (DELFT3D model) has been used to simulate the morphological behaviour of the sand wave in the North Sea. This model approach is based on the numerical solution of the three-dimensional shallow water equations in combination with a surface wave propagation model (wind waves) and the advection–diffusion equation for the sediment particles with online bed updating after each time step. The model results show that the sand wave grows in the case of dominant bed-load transport (weak tidal currents; relatively coarse sediment; small roughness height; low waves) and that the sand wave decays in the case of dominant suspended transport (strong currents, relatively fine sediment, large roughness height; storm waves).     

Calibration of LRFD Format for Steel Jacket Offshore Platforms in China Offshore Area （ 1 ） ： Statistical Parameters of Loads and Resistances

1 .IntroductionThe structural design method has evolvedfromthe workingstress method,damage stage methodtolimit state method.The more recent probabilisticlimit state design method,whichis based onreliabili-tytheory,has beengenerallyacceptedinthe designcode…     

Thorium isotopes as tracers of particles dynamics and deep water circulation in the Indian sector of the Southern Ocean (ANTARES IV)

Dissolved and particulate samples were collected to study the distribution of thorium isotopes (²³⁴Th, ²³²Th and ²³⁰Th) in the water column of the Indian sector of the Southern Ocean (from 42°S to 47°S and from 60°E to 66°E, north of the Polar Front) during Austral summer 1999. Vertical profiles of excess ²³⁰Th (²³⁰Th_xs) increases linearly with depth in surface water (0–100 m) and a model was applied to estimate a residence time relative to the thorium scavenging (τ_scav). Low τ_scav in the Polar Front Zone (PFZ) are found, compared to those estimated in the Subtropical Front Zone (STZ). Changes in particle composition between the PFZ and STZ could influence the ²³⁰Th_xs scavenging efficiency and explain this difference. An innovative coupling between ²³⁴Th and ²³⁰Th_xs was then used to simultaneously constrain the settling velocities of small (0.6–60 μm) and large (above 60 μm) particles. Although the different hydrological and biogeochemical regimes visited during the ANTARES IV cruise did not explain the spatial variation of sinking velocity estimates, our results indicate that less particles may reach the seafloor north (60 ± 2 m d^− 1, station 8) than south of the Agulhas Return Current (119 ± 23 and 130 ± 5 m d^− 1 at stations 3 and 7, respectively). This information is essential for understanding particle transport and by extension, carbon export. In the deep water column, the ²³⁰Th_xs concentrations did not increase linearly with depth, probably due to lateral transport of North Atlantic Deep Water (NADW) from the Atlantic to the Indian sector, which renews the deep waters and decreases the ²³⁰Th_xs concentrations. A specific ²³⁰Th_xs transport model is applied in the deep water column and allows us to assess a “travel time” of NADW ranging from 2 to 15 years.     

Fatal attraction: synthetic musk fragrances compromise multixenobiotic defense systems in mussels

We studied interactions of nitromusk compounds musk ketone and musk xylene and polycyclic musks Galaxolide trade mark (HHCB), Celestolide trade mark (ADBI), Tetralide trade mark (AHTN), and Traseolide trade mark (AITI) with multixenobiotic resistance (mxr) transporters in gill tissue of the marine mussel Mytilus californianus (Conrad, 1837). A competitive substrate transport test with rhodamine B was used to assay modulation of transport activity by musks. All tested musks inhibited the transport activity in the low microm range as indicated by increased accumulation of rhodamine B in the tissue. Compared to known substrates of mxr transporters, the effective concentration range was similar to quinidine and about 100 times higher than verapamil. Musk ketone and musk xylene also inhibited efflux of rhodamine B from gill tissue which was loaded with the dye and subsequently incubated with these compounds. Synthetic musk compounds are persistent environmental pollutants in aquatic environments with a high potential to bioaccumulate. As potent inhibitors of mxr transporters they may also play a role as chemosensitizers that enable toxic mxr substrates to accumulate in cells of aquatic organisms.     

A social scientist's perspective on the Census of Marine Life

Over 300 natural scientists in 53 nations are taking part in the Census of Marine Life (CoML) to investigate what lived, what lives, and what will live in the oceans. The CoML is a scientific experiment that is exploring the limits of ocean science. The paper discusses the potential applications of CoML research and the mechanisms by which the potential benefits can be measured and preserved. I recommend developing and integrating policy advisory committees with the natural science activities to both maximize the benefits of the research and to avoid unintended consequences.     

Marine tether dynamics: retrieval and deployment from a heaving platform

A tether management system of a winch module in a marine environment is proposed. For the purpose of this study the subsea body is considered as a mass attached at the free end of a long tether which is wrapped around a circular drum controlled by an external torque. The winch is considered mounted on a heaving platform. The effect of the heaving platform on the motions of the drum, tether and attached mass are studied with respect to the longitudinal vibrations of the tether in one dimension. The hydrodynamic effects are considered on the deployed portion of the tether and the tethered mass, both of which are assumed submerged in otherwise still water. The resulting nonlinear system of equations of motion is developed and solved numerically for an example of a marine tether system. The effect of the tether extensibility on the operation threshold of the system is identified. Comparison with an inextensible tether case is provided. The numerical results and their analysis for the retrieval/deployment of the system are presented.     

Effect of Pollutants on the Physical and Engineering Behavior of Lime-Treated Marine Clay

Rapid industrial growth and increasing population has resulted in the discharge of wastes into the ocean, wastes which ultimately reach the seabed and contaminate the marine sediments. The soil-contaminants interaction, and their associated physico chemical properties with sediments control the behavior of marine clays. Marine clay deposits of low strength and high compressibility are located in many coastal and offshore areas. There are several foundation problems encountered in these weak marine clays. In this study, experimental work was carried out in the laboratory to stabilize soft marine clays using the lime column technique. Also the lime-induced effects on the physical and engineering behavior of marine clays in sulfate-contaminated marine environment was investigated. Consolidation tests indicate that compressibility of the lime-treated samples was reduced to 1/2-1/3 of the virgin soil after 45 days treatment. The test results also suggest that the lime column technique can be conveniently used to improve the behavior of contaminated marine clay deposits.     

先锋孤立子生成的理论平均波阻(Ⅰ).理论

根据作者导得的AfKdV方程，理论上确定了先锋孤立子生成问题的理论平均波阻，能量劈分及能量劈分比。本文的能量劈分是确定先锋孤立子生成参数的理论基础。同时，本理论确定了现有理论中的自由未知参数问题，从而使先锋孤立子生成参数得到理论预报。     

The Working Mechanism and An Analytic Method of the Deep Embedded Large Cylinder Structure

The large cylinder is a new-type structure that has been applied to harbor and offshore engineering. An analytic method of the relationship between loads and the structure displacement is developed based on the failure mode of deep embedded large cylinder structures. It can be used to calculate directly the soil resistance and the ultirnate bearing capacity of the structure under usage. A new criterion of the large cylinder structure, which discriminates the deep embedded cylinder from the shallow embedded cylinder, is defined. Model tests prove that the proposed method is feasible for the analysis of deep embedded large cylinder structures.