粘质沙雷氏菌(Serratia marcescens)发酵培养基优化的研究

采用单因素试验和正交试验,对从山东东营海岸湿地盐碱滩地土壤中筛选出的一株海洋菌种———粘质沙雷氏菌(Serratia marcescens)的发酵培养基进行优化,并进行100 L发酵罐中试放大试验的研究。确定粘质沙雷氏菌的最佳培养基配方为葡萄糖10 g/L,硫酸铵5 g/L,麸皮50 g/L,柠檬酸三钠1.0 g/L,K2HPO4.3H2O 0.3 g/L,FeSO4.7H2O 0.05 g/L,MgSO4.7H2O 0.5 g/L,pH 7.2~7.7。发酵最适温度为30℃。通过测定粘质沙雷氏菌在发酵罐中培养的生长曲线,确定发酵时间以28~30 h为宜,发酵结束后发酵液中的活菌数约为50×108个/mL。将所筛选到的粘质沙雷氏菌应用于农作物的病害防治,效果非常显著,表明是一株高活性的生物防治拮抗菌。此研究结果为高效率、低成本和工业化生产具有生物防治作用的海洋菌种制剂提供了科学依据,也为海岸湿地盐碱土的可持续开发利用提供了技术支撑。     

我国滨海湿地生态修复领域规范的现状与分析

滨海湿地是地球上十分重要的一类生态系统, 可为人类社会提供诸如调节气候、降解污染、碳汇氮汇等众多生态服务功能。近年来, 由于气候变化和围填海等开发活动的影响, 我国滨海湿地面积锐减, 功能退化, 面临多种生态问题。为应对滨海湿地退化及日益凸显的生态环境问题, 滨海湿地保护和修复工作逐渐受到重视, 滨海湿地生态修复工程项目的数量和规模也随之急剧增加。滨海湿地生态修复技术规范对滨海湿地生态修复工作具有重要意义, 然而我国滨海湿地生态修复领域规范体系尚不完善, 导致滨海湿地生态修复项目实施无序以及生态修复成效低等问题。本文全面搜集了我国滨海湿地生态修复领域规范, 对检索到的规范进行分类统计与分析, 从而揭示其存在的问题, 主要包括规范体系系统性不足、生态修复理念滞后、规范之间缺乏协调性、规范可操作性差、规范更新迟滞等。针对存在的问题提出了相应的对策建议, 为完善我国滨海湿地生态修复领域规范体系、不断推进我国生态文明建设进程提供参考。     

厦门下潭尾红树林修复区景观类型与鸟类群落动态

2018年10月—2019年9月调查厦门下潭尾红树林修复区鸟类群落变化,利用遥感图像解译红树林修复区景观格局,为修复区后续景观格局优化与鸟类保护提供技术支持。结果显示:修复区鸟类共有26科62种,鹭类(8种2047只)与鸻鹬类(16种786只)种类及数量较多。春季鸟类种数及数量最多,其中3月鸟类种数最多25种,5月数量最多828只。下潭尾修复区景观格局在低潮位与高潮位存在周期性变化,不同景观鸟类种类及数量有较大差异;鸻鹬类在低潮位时的种类较多但数量较少,高潮位时仅分布于高潮裸露湿地和建设用地。建议修复区增加鸻鹬类高潮停息的滩涂湿地及游禽类生活需要的生态塘区,进一步提高水鸟多样性。     

城镇黑臭河道治理:多水塘活水链人工湿地的应用

随着现代工业的发展,城市黑臭河道问题日趋严重,严重影响了城市形象、生态环境和居民身心健康。利用多水塘活水链人工湿地技术净化江苏常州永胜河河水水质。监测结果显示:该技术使水体总氮浓度削减了92.0%、总磷浓度削减了82.9%,处理后的水体总氮、总磷含量达到地表IV类水标准。项目年处理水量约30万t,每年以较低的成本(平均水处理费用约为0.03元/t),削减总氮、总磷和氨氮量分别为1496.6 kg、176.0 kg和1408.6 kg。通过引植多种浮叶植物、挺水植物、沉水植物,该湿地修复工程为当地营造了优美的湿地景观。多水塘活水链人工湿地是集经济效益、生态效益、环境效益于一体的新型水体生态治理技术,为我国城镇黑臭河道治理提供了一条新的途径。     

Stable Carbon Isotope and Long-Chain Alkane Compositions of the Major Plants and Sediment Organic Matter in the Yellow River Estuarine Wetlands

Elemental(TOC,TN,C/N)and stable carbon isotopic(δ^13C)compositions and long-chain alkane(n C16-38)concentrations were measured for eight major plants and a sediment core collected from the Yellow River estuarine wetlands.Our results indicate that both C3(-25.4‰to-29.6‰)and C4(-14.2‰to-15.0‰)plants are growing in the wetlands and C3 plants are the predominant species.The biomass of the wetland plants had similar organic carbon(35.5-45.8%)but very different organic nitrogen(0.35-4.15%)contents.Both C3 and C4 plants all contained long-chain alkanes with strong odd-to-even carbon numbered chain predominance.Phragmites australis,a dominant C3 plant contained mainly n C29 and n C31 homologues.Aeluropus littoralis,an abundant C4 plant were concentrated with n C27 and n C29 homologues.Organic matter preserved in the Yellow River estuarine sediments showed strong terrestrial signals(C/N=11-16,δ^13C=-22.0‰to-24.3‰).The distribution of long-chain n-alkanes in sediments also showed strong odd-to-even carbon chain predominance with n C29 and n C31 being the most abundant homologues.These results suggest that organic matter preserved in the Yellow River estuarine sediments were influenced by the wetland-derived organic matter,mainly C3 plants.The Yellow River estuarine wetland plants could play important role affecting both the carbon and nutrient cycling in the estuary and adjacent coastal waters.     

荒漠绿洲湿地土壤水热盐动态过程及其影响机制

以甘肃临泽荒漠绿洲湿地为研究对象,通过对土壤温度、含水量、电导率及蒸散量的野外观测,在植物生长期和冻融期分别深入分析水热盐耦合运移过程及其影响因素,探讨水热梯度对盐分运移及其分布格局的控制作用。结果表明：土壤温度整体呈现出春夏季逐渐升高、秋冬季降低趋势。在冻结期,土壤表现为脱盐状态,表层电导率由2.8 mS·cm^-1降到1.2 mS·cm^-1;而在消融期为积盐状态,表层电导率由1.2 mS·cm^-1升到3.7 mS·cm^-1。在生长期,土壤含水量和电导率波动较为剧烈,表层含水量27%~43%,表层电导率3~5.5 mS·cm^-1,土壤脱盐、积盐反复出现。全年蒸散量总体呈单峰变化趋势,年蒸散量507 mm;土壤电导率与蒸散量呈正比关系,与地下水位呈负相关关系;蒸散发作用是土壤表层积盐的主要驱动力,而地下水波动影响着湿地脱盐、洗盐过程。因此,荒漠绿洲湿地土壤盐分累积过程是水分运移和热量传输过程发生变化的结果。     

Impacts of Coastal Reclamation on Natural Wetlands in Large River Deltas in China

Little information is available on the impacts of coastal reclamation on wetland loss in large-river deltas at a regional scale.Using remote sensing data of coastal wetland and reclamation in four deltas in China from 1978 to 2014, we tracked their continuous area changes in four periods: 1978–1990, 1990–2000, 2000–2008, and 2008–2014. The areal relation between wetland loss and reclamation was quantified and used to identify coastal reclamation mode intensity coupled with another three indicators: reclamation rate,accretion rate and land-use intensity of coastal reclamation. The results showed that coastal reclamation driven by economic development reduced, or even reverse the original growth of delta which was determined by the offset between wetland acceleration rate and wetland loss rate. Generally, the area of reclamation showed a positive linear correlation with the area of wetland loss. The findings imply that human activities should control reclamation rate and intensity to alleviate total wetland loss and maintain wetland ’net gain’.Inappropriate coastal reclamation modes can magnify total wetland loss;therefore, coastal reclamation with a slow increment rate and low impervious surface percent is of great importance for sustainable development in future coastal management.     

不同水位垂直流人工湿地中植物及微生物特征

利用4个连续进水的垂直流人工湿地,比较分析水位对污染物去除效果的影响,研究湿地中植物对氮磷去除的贡献,阐析湿地中脱氮功能菌数量的演变规律。3个湿地栽种黄花鸢尾,水位分别控制在19、51和84cm,另一个湿地不栽种植物,水位为51cm。结果表明,水位对氮和有机物的去除有显著影响(p<0.05),栽种植物的湿地中,51cm水位时总氮去除率(67.4%～79.2%)最高,19cm水位时氨氮(85.3%～93.0%)和COD(81.8%～92.9%)去除效果最好。试验中黄花鸢尾均生长良好,植物吸收对总氮(Total nitrogen,简称TN)和总磷(Total phosphorus,简称TP)去除的贡献分别为19.2%～27.3%和14.7%～19.2%;植物地上部分发挥更重要作用,其TN和TP含量及对TN和TP的吸收量均高于地下部分。湿地表层基质中3种脱氮功能菌数量均随运行时间的增加而显著提高,亚硝化细菌和硝化细菌数量分别为10~4～10~6和10~5～10~7 MPN/g,随水位升高而减少;反硝化细菌数量为10~3～10~6 MPN/g,随水位升高而增加。     

湿地资源调查更新若干问题探讨

对古浪县第二次全国湿地资源调查的技术、结果进行了系统分析,探索了研究区内湿地资源调查更新的方法,通过"天空地"协同和"互联网+"的调查更新技术全面更新研究区内湿地资源调查数据库,以全面掌握湿地资源状况。研究结果:1)古浪县第二次湿地资源调查范围内的非湿地类型面积达4 615.35 hm2,占全县第二次湿地调查总面积的59.31%。2)变更后县域内湿地资源总面积4 131.54 hm2,以河流水面、内陆滩涂、水库水面、沟渠为主要用地类型,较第二次调查湿地面积净减少3 650.25 hm2。3)"天空地"协同遥感监测技术为湿地资源变更监测提供高精度多源数据,保证了调查、监测的精度和准确性。     

Impacts of soil fauna on litter decomposition at different succession stages of wetland in Sanjiang Plain, China

Litter decomposition is the key process in nutrient recycling and energy flow. The present study examined the impacts of soil fauna on decomposition rates and nutrient fluxes at three succession stages of wetland in the Sanjiang Plain, China using different mesh litterbags. The results show that in each succession stage of wetland, soil fauna can obviously increase litter decomposition rates. The average contribution of whole soil fauna to litter mass loss was 35.35%. The more complex the soil fauna group, the more significant the role of soil fauna. The average loss of three types of litter in the 4mm mesh litterbags was 0.3–4.1 times that in 0.058mm ones. The decomposition function of soil fauna to litter mass changed with the wetland succession. The average contribution of soil fauna to litter loss firstly decreased from 34.96% (Carex lasiocapa) to 32.94% (Carex meyeriana), then increased to 38.16% (Calamagrostics angustifolia). The contributions of soil fauna to litter decomposition rates vary according to the litter substrata, soil fauna communities and seasons. Significant effects were respectively found in August and July on C. angustifolia and C. lasiocapa, while in June and August on C. meyeriana. Total carbon (TC), total nitrogen (TN) and total phosphorus (TP) contents and the C/N and C/P ratios of decaying litter can be influenced by soil fauna. At different wetland succession stages, the effects of soil fauna on nutrient elements also differ greatly, which shows the significant difference of influencing element types and degrees. Soil fauna communities strongly influenced the TC and TP concentrations of C. meyeriana litter, and TP content of C. lasiocapa. Our results indicate that soil fauna have important effects on litter decomposition and this influence will vary with the wetland succession and seasonal variation. Foundation item: Under the auspices of State Key Development Program for Basic Research of China (No. 2009CB421103), Key Program of National Natural Science Foundation of China (No. 40830535/D0101), Knowledge Innovation Programs of Chinese Academy of Sciences (No. KZCX2-YW-BR-16, KSCX2-YW-N-46-06)