全文获取类型
收费全文 | 241篇 |
免费 | 21篇 |
国内免费 | 37篇 |
专业分类
测绘学 | 110篇 |
大气科学 | 9篇 |
地球物理 | 18篇 |
地质学 | 35篇 |
海洋学 | 75篇 |
天文学 | 2篇 |
综合类 | 42篇 |
自然地理 | 8篇 |
出版年
2022年 | 4篇 |
2021年 | 6篇 |
2020年 | 6篇 |
2019年 | 7篇 |
2018年 | 2篇 |
2017年 | 19篇 |
2016年 | 13篇 |
2015年 | 11篇 |
2014年 | 14篇 |
2013年 | 21篇 |
2012年 | 24篇 |
2011年 | 14篇 |
2010年 | 15篇 |
2009年 | 15篇 |
2008年 | 15篇 |
2007年 | 11篇 |
2006年 | 28篇 |
2005年 | 13篇 |
2004年 | 13篇 |
2003年 | 11篇 |
2002年 | 9篇 |
2001年 | 9篇 |
2000年 | 4篇 |
1999年 | 3篇 |
1998年 | 5篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1991年 | 1篇 |
排序方式: 共有299条查询结果,搜索用时 15 毫秒
91.
92.
93.
A muscle cDNA library of Chinese shrimp (Fenneropenaeus chinensis) was constructed with the SMART™ cDNA Library Construction Kit. The titer of optimal primary library was 7.7×105 pfu mL−1 and that of the amplified library was 3.0×109 pfu mL−1. The percentages of the recombinant clones of primary and amplified libraries were over 98%. The insert sizes were longer
than 400 bp with an average of 1000 bp. A positive clone containing a 794 bp insert was sequenced and identified encoding
fast skeletal troponin I gene. This library provided a useful resource for the functional genomic research of F. chinensis. 相似文献
94.
A muscle cDNA library of Chinese shrimp (Fenneropenaeus chinensis) was constructed with the SMARTTM cDNA Li- brary Construction Kit. The titer of optimal primary library was 7.7×105 pfu mL-1 and that of the amplified library was 3.0×109 pfu mL-1. The percentages of the recombinant clones of primary and amplified libraries were over 98%. The insert sizes were longer than 400 bp with an average of 1000 bp. A positive clone containing a 794 bp insert was sequenced and identified encoding fast skeletal troponin Ⅰ gene. This library provided a useful resource for the functional genomic research ofE chinensis. 相似文献
95.
“物化探技术方法库”是“中国地质调查技术方法信息网”的有机组成部分。通过研究,将物探方法归纳为重力、磁法、电法、地震、放射性和地温6大类25种方法,将化探方法归纳为沉积物、土壤、岩石、气体、水、生物和其他等7大类16种方法。对每种技术方法均作了概要性的简介;经筛选,编制入库物化探技术方法、仪器设备、软件及应用实例等信息条目208条,初步充实了物化探技术方法库的内容,为服务平台物化探方法技术库运行提供了基本条件。 相似文献
96.
Effects of exposure to polychlorinated biphenyls (PCBs) on Sebastiscus marmoratus were investigated using a suppression subtractive hybridization method. A total of 108 gene sequences were identified as having the potential for being differentially expressed, and 45 could be identified with homologous database sequences. Functions with which they were associated included long-term potentiation and neurotransmitter release, neuroendocrine, mitosis and cell proliferation, energy-related metabolism, general metabolism, signal protein, hemopoiesis system, immune system, and structure. The expression of 17 of these genes was analyzed in the brain using real time fluorescent quantitative PCR. The present study provided a basis for studying the response of fish to PCB exposure and allowed the characterization of new potential neurotoxicol biomarkers of PCB contamination in seawater. 相似文献
97.
Wen Zhang Lin-sheng Song Jang-Seu Ki Chun-Kwan Lau Xiang-Dong Li Pei-Yuan Qian 《Estuarine, Coastal and Shelf Science》2008
Sulfate-reducing bacteria (SRB) are important regulators of a variety of processes in coastal marine sediments regarding organic matter turnover, biodegradation of pollutants, and sulfur and carbon cycles. Yet their community compositions have not been investigated in polluted harbor sediments. This study described the diversity and spatial variation of SRB communities in surface sediments in Victoria Harbor, Hong Kong. The spatial variation of SRB communities was described by terminal restriction fragment length polymorphism (T-RFLP). The results showed that the most diversified terminal restriction fragments were found at polluted sites. In addition, cluster analysis indicated that although the SRB communities were different at the two polluted sites, they were still more similar to each other than to the two more distant reference sites. Based on a dsrAB clone library constructed at a polluted site, diversified SRB were found, represented by 30 Operational Taxonomic Units (OTUs). Upon comparisons among the SRB sequences detected from this study and those in the GenBank, five clades of SRB were found. Three clades belonged to the known families Desulfobacteraceae, Desulfobulbaceae, and Syntrophobacteriaceae. The majority of sequenced clones, which distantly related to sequences in the GenBank, constituted the remaining two unclassified groups, suggesting unique SRB members related to the polluted harbor environment. Statistical analyses indicated that estimated SRB richness correlated with environment factors such as sulfur content, acid volatile sulfate, and redox potential. 相似文献
98.
知识经济是近年来世界范围内兴起的一个新概念。阐述了知识经济时代图情工作面临的机遇和挑战 ,同时阐述了图情工作在知识经济发展中的地位和作用。 相似文献
99.
插件技术在天气雷达数据处理软件中的应用 总被引:4,自引:2,他引:4
介绍了在多普勒天气雷达二次产品软件的编写中使用动态链接库(Dynam—ic—link Library)技术设计的一个插件系统(Plug—in System)。该系统中所有的算法都设计成插件,将产品算法部分和系统本身分离,便于工程的管理和进一步开发,使研究人员能够集中精力致力于算法的实现。该系统本身也可以作为一个新算法、新产品的开发平台。 相似文献
100.
Construction of a metagenomic DNA library of sponge symbionts and screening of antibacterial metabolites 总被引:1,自引:0,他引:1
CHEN Juan ZHU Tianjiao LI Dehai CUI Chengbin FANG Yuchun LIU Hongbing LIU Peipei GU Qianqun ZHU Weiming 《中国海洋大学学报(英文版)》2006,5(2):119-122
To study the bioactive metabolites produced by sponge-derived uncultured symbionts, a metagenomic DNA library of the symbionts of sponge Gelliodes gracilis was constructed. The average size of DNA inserts in the library was 20 kb. This library was screened for antibiotic activity using paper disc assaying. Two clones displayed the antibacterial activity against Micrococcus tetragenus. The metabolites of these two clones were analyzed through HPLC. The result showed that their metabolites were quite different from those of the host E. coli DH5α and the host containing vector pHZ132. This study may present a new approach to exploring bioactive metabolites of sponge symbionts. 相似文献