Oxygen radical formation in anoxic transgression and anoxia-reoxygenation: Foe or phantom? Experiments with a hypoxia tolerant bivalve

Intertidal blue mussels, Mytilus edulis, experience hypoxia reoxygenation during tidal emersion and resubmersion cycles, and this is often suggested to represent a major stress for the animals, especially for their respiratory tissues, the gills. We exposed mussels to experimental short and prolonged anoxia and subsequent reoxygenation and analyzed the respiratory response in excised gill tissue and the effects of treatment on reactive oxygen species (mainly ROS: superoxide anion, O₂^·− and hydrogen peroxide, H₂O₂), formation using live imaging techniques and confocal microscopy. Our aim was to understand if this “natural stress” would indeed produce oxidative damage and whether antioxidant defenses are induced under anoxia, to prevent oxidative damage during reoxygenation. Exposure to declining pO₂ in the respiration chamber caused an increase of gill metabolic rate between 21 and 10 kPa, a pO₂ range in which whole animal respiration is reported to be oxyregulating. Exposure of the animals to severe anoxia caused an onset of anaerobiosis (succinate accumulation) and shifted high and low critical p_c values (p_c1: onset of oxyregulation in gills, p_c2: switch from oxyregulation to oxyconformity) to higher pO₂. Concentrations of both ROS decreased strongly during anoxic exposure of the mussels and increased upon reoxygenation. This ROS burst induced lipid peroxidation in the mantle, but neither were protein carbonyl levels increased (oxidative damage in the protein fraction), nor did the tissue glutathione concentration change in the gills. Further, analysis of apoptosis markers indicated no induction of cell death in the gills. To our knowledge, this is the first paper that directly measures ROS formation during anoxia reoxygenation in mussels. We conclude that hypoxia tolerant intertidal mussels do not suffer major oxidative stress in gill and mantle tissues under these experimental conditions.     

南海珠江口盆地东沙隆起区生物礁演化模式

南海是西太平洋最大的边缘海,其在形成演化过程中出现了有利于生物礁生长发育的环境。东沙隆起在中新世时期属于浅海环境,远离物源区,温度、盐度和水深适宜生物礁的生长和发育。通过地震资料的解释、测井、岩心资料的综合分析,并与相对海平面变化曲线对比,认为东沙隆起发育大量规模不等的生物礁,且其发育演化与相对海平面变化曲线和区域构造演化阶段可以对应起来。相对海平面的旋回性变化和复杂的构造演化过程结合,使得研究区生物礁具有较好的孔渗性,可以成为有潜力的油气储层。     

Tackling illegal, unregulated, and unreported trade towards Humphead wrasse (Cheilinus undulatus) recovery in Sabah, Malaysia

The live reef food fish trade (LRFFT) has caused severe depletion of the Humphead wrasse (Cheilinus undulatus) populations globally, a species considered endangered by the International Union for the Conservation of Nature. In 2010, signatories to the Convention on International Trade in Endangered Species of Wild Fauna and Flora recognized that illegal, unregulated, and unreported (IUU) activities in the LRFFT were undermining the efforts to protect this species. Management recommendations have yet to be effectively implemented at national and local levels where the fish is harvested. This paper analyzes the threats to, and socio-economic impacts of, Humphead wrasse recovery in Sabah, Malaysia, and offers both short- and longer-term recommendations for the local recovery of the species. Key issues addressed centre around understanding the threats to recovery, specifically the role of IUU and the context-specific considerations, including the socio-economic aspects of the IUU trade in Sabah. The analysis confirms that deliberate management planning and action at multiple levels are required to prevent local-scale extinction. Lessons for other species and countries involved in the LRFFT suggest that to be effective, broad management frameworks must be tailored to local situations and must focus on gaining support and participation of fish harvesters if the recovery and long-term sustainability of both the Humphead wrasse and the LRFFT are to be achieved.     

Amphipod-supported food web: Themisto gaudichaudii, a key food resource for fishes in the southern Patagonian Shelf

The trophic role of the hyperiid amphipod Themisto gaudichaudii in the southern Patagonian shelf food web was assessed from the analysis of stomach contents of the local fish assemblage. A total of 461 trawl samples were collected during seven seasonal cruises. A total of 17 out of 38 fish species were found to ingest T. gaudichaudii. This amphipod was a main prey item in five of these species, showing high values of alimentary index: Seriolella porosa (99.9%), Macruronus magellanicus (68.8%), Micromesistius australis (59.1%), Patagonotothen ramsayi (48.6%), and Merluccius hubbsi (10.9%). The contribution of T. gaudichaudii, in weight, to their summer diet was 60%, on average. This contribution was minimal in winter and maximal in summer. Fisheries studies have indicated that these five species, mainly M. magellanicus, account for almost 85% of the fish biomass in the area. Although the remaining 15% did not feed heavily on T. gaudichaudii, they are known to prey on the main hyperiid predators. Our study shows that T. gaudichaudii contributes greatly, both directly and indirectly, to supporting the fish community. We thus proposed that T. gaudichaudii plays a key role as a “wasp-waist” species in the sub-Antarctic region, similar to that of krill in Antarctic waters, channeling the energy flow and enabling a short and efficient food chain.     

Long-term changes in coral assemblages under natural and anthropogenic stress in Jakarta Bay (1920-2005)

Coral reefs in Jakarta Bay have been subjected to scientific studies since the 1920s. Also from that time on biological collections were made. The reefs in the Jakarta Bay have been under long-term natural and anthropogenic stress. With the biological collections and historical documents the coral species richness in Jakarta Bay around 1920 was reconstructed. New data from this bay and the adjacent offshore Thousand Islands archipelago were obtained during a 2005 research expedition. A comparison of the coral assemblages between 1920 and 2005 reveals a clear decline in species numbers. The most prominent results include the near-shore disappearance of species belonging to the families Acroporidae, Milleporidae, and to a lesser extent Poritidae. The overall coral species composition of the reefs has changed considerably, which is partly reflected in a strong decline in coral species richness. About half the number of species recorded in 1920 was found again in 2005.     

Tropical harmful algal blooms: An emerging threat to coral reef communities?

Tropical harmful algal blooms (HABs) are increasing in frequency and intensity and are substantially affecting marine communities. In October/November 2008 a large-scale HAB event (> 500 km², dinoflagellate Cochlodinium polykrikoides) in the Gulf of Oman caused the complete loss of the branching corals, Pocillopora and Acropora spp., and substantial reductions in the abundance, richness and trophic diversity of the associated coral reef fish communities. Although the causative agents of this C. polykrikoides bloom are unknown, increased coastal enrichment, natural oceanographic mechanisms, and the recent expansion of this species within ballast water discharge are expected to be the main agents. With rapid changes in oceanic climate, enhanced coastal eutrophication and increased global distribution of HAB species within ballast water, large-scale HAB events are predicted to increase dramatically in both intensity and distribution and can be expected to have increasingly negative effects on coral reef communities globally.     

基于空间句法的商品交易市场空间结构——以义乌国际商贸城为例

空间句法作为一种定量空间形态分析方法,广泛应用于城市设计和建筑的诸多方面,但是在商业建筑结构方面的应用研究较少。本文运用GIS和空间句法的集成方法定量分析了义乌国际商贸城内部的空间结构特征,探讨了空间句法在商品交易市场空间结构形态研究中的应用。研究结果表明：义乌商贸城内部空间结构在总体布局上智能性、可理解性较差,局部区域及部分入口的便捷度较差,部分辅助设施不易达;针对这些不足本文也提出相应的优化调整建议。另外,该研究也可为商品交易市场的规划设计提供一定的参考。     

Maquiladora Myths: Locational and Structural Change in Mexico's Export Manufacturing Industry

Trends in location, labor force, and procurement practices in maquiladoras are examined using recent data sources. A growing proportion of maquiladoras are selecting interior locations, south of the borderlands. Once dominated by young women, the labor force is rapidly approaching gender parity. While far below prevailing rates in the United States, maquiladora wages are comparable with equivalent manufacturing sectors in Mexico. Majority ownership of maquiladoras is split almost evenly between Mexico and the U.S., however, maquiladoras have failed to develop domestic sources of materials and parts and remain dependent on imported material inputs. As the North American Free Trade Agreement is phased in, the regulatory environment of maquiladoras will change but their role as low cost assembly specialists will persist.     

Forthcoming events

Tropical cyclones (hurricanes and typhoons) produce high winds that can generate waves capable of damaging coral reefs. As cyclones frequently pass through northeast Australia's Great Barrier Reef (GBR), it is important to understand how the spatial distribution of reef damage changes over time. However, direct measurements of wave damage, or even wave heights or wind speeds, are rare within the GBR. An important factor in estimating whether cyclone damage was possible is the magnitude and duration of high‐energy wind and waves. Thus, before the spatio‐temporal dynamics of past cyclone damage can be modelled, it is necessary to reconstruct the spread, intensity, and duration of high‐energy conditions during individual cyclones. This was done every hour along the track taken by each of 85 cyclones that passed near the GBR from 1969 to 2003, by implementing a cyclone wind hindcasting model directly within a raster GIS using cyclone data available from the Australian Bureau of Meteorology. Three measures of cyclone energy (maximum wind speed—MAX, duration of gales—GALES, and continuous duration of gales—CGALES) were derived from these data. For three cyclones, where field data documenting actual reef damage from cyclone‐generated waves were available, the predictive ability of each measure was assessed statistically. All three performed better in predicting reef damage at sites surveyed along the high‐energy reef front than those surveyed along the more protected reef back. MAX performed best for cyclone Joy (r ² = 0.5), while CGALES performed best for cyclones Ivor (r ² = 0.23) and Justin (r ² = 0.48). Using thresholds for MAX and GALES obtained via comparison with field data of damage, it was possible to produce a preliminary prediction of the risk of wave damage across the GBR from each of the 85 cyclones. The results suggest that while up to two‐thirds of the GBR was at risk from some damage for 30–50% of the time series (～18 out of 35 years), only scattered areas of the region were at risk more frequently than that.     

Pressure-specific and multiple pressure response of fish assemblages in European running waters

We classified homogenous river types across Europe and searched for fish metrics qualified to show responses to specific pressures (hydromorphological pressures or water quality pressures) vs. multiple pressures in these river types. We analysed fish taxa lists from 3105 sites in 16 ecoregions and 14 countries. Sites were pre-classified for 15 selected pressures to separate unimpacted from impacted sites. Hierarchical cluster analysis was used to split unimpacted sites into four homogenous river types based on species composition and geographical location. Classification trees were employed to predict associated river types for impacted sites with four environmental variables. We defined a set of 129 candidate fish metrics to select the best reacting metrics for each river type. The candidate metrics represented tolerances/intolerances of species associated with six metric types: habitat, migration, water quality sensitivity, reproduction, trophic level and biodiversity. The results showed that 17 uncorrelated metrics reacted to pressures in the four river types. Metrics responded specifically to water quality pressures and hydromorphological pressures in three river types and to multiple pressures in all river types. Four metrics associated with water quality sensitivity showed a significant reaction in up to three river types, whereas 13 metrics were specific to individual river types. Our results contribute to the better understanding of fish assemblage response to human pressures at a pan-European scale. The results are especially important for European river management and restoration, as it is necessary to uncover underlying processes and effects of human pressures on aquatic communities.