Potential anticancer activity analysis of piscidin 5-like from Larimichthys crocea

Antitumor activity is one characteristic function of some certain antimicrobial peptides(AMPs) found in recent years. In the present study, we attempted to detect potential anticancer activity of a recombinant piscidin 5-like from Larimichthys crocea(r Lc-P5L) which owned widely antibacterial and strong antiparasitic activity in vitro.The light microscope observation indicated r Lc-P5L was of antitumor activity to He La cells, 293 T cells and L929 cells. MTT assay showed the toxic sensitivity of...     

小黄鱼(Larimichthys polyactis)鰤鱼诺卡氏菌的分离及鉴定

为探明舟山海域网箱养殖小黄鱼(Larimichthys polyactis)暴发的以体表溃疡、眼球溃烂、内脏出现白色结节为主要症状的疾病病因, 对患病小黄鱼体表溃疡处和结节病灶处采集组织样本, 在血琼脂平板上划线培养, 通过分离、纯化到同一优势菌株ZHNK2101。经形态学观察、生理生化鉴定、16S rRNA基因片段扩增及序列比对,确定该菌株为鰤鱼诺卡氏菌(Nocardia seriolae)。药敏试验结果显示, 菌株ZHNK2101对哌拉西林、头孢曲松、丁胺卡那、庆大霉素等16种抗生素敏感。回归感染试验表明, 该菌株可以感染小黄鱼并引起和自然感染相同的症状, 半致死浓度LD₅₀为7.28×10⁴ CFU/尾。另外,该病株会引起小黄鱼鳃、肝、肾和脾脏发生病理变化, 主要表现为鳃丝紊乱,肝脏、肾脏、脾脏组织细胞纤维化形成病理性结节。试验首次报道了小黄鱼感染鰤鱼诺卡氏菌的病例, 并从理化特性、药敏试验、回归感染及组织病理变化等方面对该菌株的特性进行了初步研究, 为小黄鱼养殖过程中由鰤鱼诺卡氏菌引起的内脏白点病的早期预防和治疗提供基础数据。     

养殖大黄鱼生化组份的分析

本文测定了厦门火烧屿养殖大黄鱼成、幼鱼肌肉中蛋白质、脂类和糖类等生化组份,并计算其比能值;分析了肌肉蛋白南中１７种常见氨基酸的含量。结果表明：成鱼肌肉蛋白质占１８．９％（湿重比,下同）脂类为３．１１％,糖类为１．６２％,水份占７５．３０％,灰份为０．９５％。比能值为５．９７ＫＪ／ｇ,Ｅ／Ｐ值为３１．６２ＫＪ／ｇ。幼鱼肌肉中占７５．３０％,灰份为０．９５％。比值为５．９８ＫＪ／ｇ,Ｅ／Ｐ值为３１．６     

2020—2021年舟山渔场及邻近海域小黄鱼繁殖力现状

为掌握舟山渔场及周边水域小黄鱼（Larimichthys polyactis）的繁殖特性,文章根据2020年和2021年3—5月在舟山渔场及邻近海域进行的拖网和流刺网渔获样品,分析了小黄鱼繁殖群体特征,及其繁殖力与生长指标的关系,并对繁殖力变化做了比较分析。结果表明：2020—2021年小黄鱼群体体长范围为107~20...     

Immune response of Pseudosciaena crocea to the injection of Vibrio alginolyticus

For the investigation of anti-infection immune response of Pseudosciaena crocea, 160 healthy fish samples were categorized into infected and control groups. Each individual fish in the infected group was injected intraperitoneally (i.p.) with 0.2 ml bacterial suspension of Vibrio alginolyticus in density of 2×107 CFU/ml, while each individual in the control group was injected i.p. with 0.2 ml sterile saline solution (0.85%). It was observed that the artificial injection of V. alginolyticus significantly inc...     

网箱养殖大黄鱼(Pseudosciaena crocea)疾病与环境因子的关系

根据2001-2005年对舟山市网箱养殖大黄鱼的养殖情况、发病情况、死亡情况和海区环境因子的监测以及收集的气象资料,对疾病与环境因子的关系进行了研究,探索了环境因子及其变化对大黄鱼发病率的影响.结果表明,大黄鱼的发病率和发病类型与气温、风速有关:发病率与水温(>20℃)、悬浮物、化学耗氧量(chemical oxygen depletion,COD)、细菌数均呈显著的正相关,与透明度呈显著的负相关.通径分析显示,对大黄鱼发病率直接效应最大的是水温,其次是风速.通过分析,推测引起舟山市网箱养殖大黄鱼疾病多发的主要原因是网箱养殖本身造成的水体污染,从而提出了舟山市网箱养殖大黄鱼疾病预防的主要措施.     

Single nucleotide polymorphisms in intron 1 and intron 2 of Larimichthys crocea growth hormone gene are correlated with growth traits

The growth hormone gene (GH) affects animal growth and is a potential target for genetic studies of variation related to growth traits. In this study, we analyzed single nucleotide polymorphisms (SNPs) in GH intron regions and their associations with growth traits in large yellow croaker, Larimichthys crocea, from Zhejiang and Fujian stocks. The results of PCR-single strand conformation polymorphism showed two haplotypes of intron 1, named AA and AB genotypes, in Zhejiang stock. AB exhibited an SNP at position 196 (G A) that was negatively correlated with body height and positively correlated with standard length/body height (P 0.05). Two different genotypes, CC and CD, were identified in intron 2 in Fujian stock, with CD showing an SNP at position 692 (T C). The CD genotype had a significantly positive correlation with both weight and total length (P 0.01). These basic data highlight the potential for using GH as a genetic marker of fish growth in marker assisted selection.     

病原性溶藻弧菌对大黄鱼鳃黏液黏附作用研究

采用3H-TdR同位素示踪方法研究了溶藻弧菌对大黄鱼鳃黏液黏附作用.结果表明,溶藻弧菌的黏附量随着菌浓度的增加而升高并在180 min后达到饱和;在温度15~30℃、pH偏酸条件下黏附作用较强,Na+是溶藻弧菌黏附作用必需的,Ca2+起着辅助作用;溶藻弧菌经营养饥饿、热处理及高碘酸处理后黏附作用有明显下降;8种碳水化合物都有不同程度的促进溶藻弧菌的黏附作用,其中葡萄糖、甘露糖、果糖及麦芽糖有明显促进作用.研究结果表明:海水中的溶藻弧菌可很好地黏附于大黄鱼鳃黏液,其黏附作用受环境因子影响,并且与溶藻弧菌的表面碳水化合物、热敏结构等有着密切关系.     

健康与患病大黄鱼消化道微生物结构特征分析

为研究患病大黄鱼(Larimichthys crocea)消化道中可能的病原微生物,采用高通量测序技术详细分析了健康和患病大黄鱼的胃、幽门盲囊和肠道中微生物群的组成信息和分布特征.研究显示,变形菌门、拟杆菌门和厚壁菌门为健康和患病大黄鱼消化道的优势菌门,且其丰度在两类鱼消化道中差异显著.拟杆菌属和乳杆菌属为健康大黄鱼消...     

大黄鱼(Larimichthys crocea)I型干扰素基因的特征与表达分析

采用RT-PCR和RACE-PCR技术克隆了大黄鱼(Larimichthys crocea)Ⅰ型干扰素基因全长cDNA序列及其基因组序列,并利用荧光定量PCR技术研究了该基因在大黄鱼不同组织中的表达谱,以及副溶血弧菌(Vibrio parahaemolyticus)、LPS、polyI:C刺激后大黄鱼脾脏、肝脏和头肾组织中该基因在转录水平的表达变化。结果表明,大黄鱼Ⅰ型IFN基因全长cDNA为878bp,开放阅读框558bp,编码185个氨基酸。推测的大黄鱼Ⅰ型IFN氨基酸序列N端含有20个氨基酸的信号肽,其后包含一个保守的IFabd结构域。大黄鱼Ⅰ型IFN基因包含5个外显子,4个内含子,在大多数组织和细胞中都有表达,其中在血细胞中表达量最高,肌肉中表达量最低。PolyI:C刺激可诱导大黄鱼Ⅰ型干扰素基因在脾脏、头肾和肝脏中的表达量显著上调;LPS刺激可诱导大黄鱼Ⅰ型干扰素基因在脾脏和肝脏中的表达量显著上调;灭活的副溶血弧菌可诱导大黄鱼Ⅰ型干扰素基因在头肾和肝脏中的表达量显著上调。