植物激素对海带配子体克隆附着的影响

实验研究 2 ,4 - D、KT、IAA、NAA四种植物激素对海带配子体克隆附着的影响。结果表明 :2 ,4 - D对附着有较明显的促附着作用 ,最有效浓度为 2 .5mg/L 和 5mg/L,其 4 h的附着率分别是对照的 2 .79倍和 2 .95倍 ;KT的作用较复杂 ,在浓度为 1mg/L 时 ,对附着有明显的促进作用 ,其 4 h的附着率是对照的 2 .3倍 ;IAA对配子体附着没有明显的促进或抑制作用 ;NAA对附着有抑制作用 ,浓度越高 ,抑制作用越强。     

海带多酚的产生与抗褐藻酸降解菌感染的相关性

利用生物化学的方法探讨了褐藻酸降解菌感染过程中海带体内多酚含量的变化 ,并分析了多酚含量的高低与海带对褐藻酸降解菌感染抗性的相关性。研究结果表明 :褐藻酸降解菌感染下海带体内多酚含量急剧上升 ,并且这种变化具有普遍性 ;多酚含量的急剧上升只发生在褐藻酸降解菌感染的初期阶段 ;海带体内多酚含量的高低与海带对褐藻酸降解菌的抗性具有明显的相关性。表明多酚类化合物在海带抵抗褐藻酸降解菌的感染中起重要作用     

Effects of blue light on gametophyte development of Laminaria japonica （Laminariales, Phaeophyta）

1 INTRODUCTION Light acts as an important ambient factor to regulate plant growth. Several critical points in life history for many marine algae are controlled by light. Photobiology of algae has been a discipline of particular interest for a long time (Dring, 1988). Many light effects on algae are photoperi- odic-regulated, such as sporangia formation in red algae Porphyra tenera (Rentschler, 1967) and As- paragopsis armata (Oza, 1976), and erect thalli de- velopment in brown algae Scy…     

Immunosuppressive Activities of Fucoidan from Laminaria japonica

Effects of fucoidan from Laminariajaponica on 2,4-dinitrochlorobenzene induced de-layed-type hypersensitivity (DTH) reaction and the serum levels of IgG, IgM, complement C3 and C4 were investigated in the present study. Results showed that oral administration of fucoidan atdose of 1.50 and 300 mg/(kg*d) for 9 days before the hapten challenge significantly inhibited 2,4-dinitrochlorobenzene induced delayed-type hypersensitivity reaction; and also inhibited the humoral immunity. Serum C3 and C4 levels were markedly reduced by fucoidan at dose of 1.50 and 300 mg/kg; and serum IgG and IgM levels were reduced by fucoidan at dose of 300 mg/kg. The inhibitory effects of fucoidan on delayed-type hypersensitivity suggested that it may be potential medication for chronic inflammatory diseases in the future.     

The extraction of pigments from fresh <Emphasis Type="Italic">Laminaria japonica</Emphasis>

The pigments in Laminariajaponica was extracted with six organic solvents and analyzed in spectroscopy analysis. The extractions conditions were screened by an orthogonal test and the quantity of extracted pigments was determined spectroscopically. The results show that： （1） among the six organic solvents, acetone was the most effective one for the extraction; （2） the optimum extraction conditions were as follows： the ratio of S/M （solvent volume/ material weight） was 30 ml/g; fresh seaweed was extracted 2 times in 2 h; （3） the average total content of pigments was 1.85 mg/g （calculated with dry L. japonica）.     

褐藻酸降解酶特性的初步研究

对从海带(Laminaria japonica)病烂处分离出的2株别单胞菌属的褐藻酸降解菌(简称菌株A1,A2)进行了褐藻酸钠降解能力及胞外产物中褐藻酸酶特性的初步分析。结果表明,2株褐藻酸降解菌中,菌株A2表现出较强的对褐藻酸钠的降解能力;同时菌株A2所分泌的褐藻酸酶在pH 6.0~8.0相对稳定,30℃以下酶不易失活。在较低离子浓度时,Mn2 ,Ba2 对反应有较强的促进作用,Ag 和Pb2 则有明显的抑制作用。     

海带愈伤组织的高效率诱导

于１９９４年１月和１１月在青岛太平角和荣成海带育苗场分别采集海带成熟孢子体和幼孢子体,采用培养基诱导方法,以４种培养基诱导海带愈伤组织的效果,成熟海带孢子体的不同部位在ＭＳ固体增富２基中形成愈 组织的能力进行了比较;研究了添另不同成分的ＭＳ固体增富２基对海带愈伤组织的影响,并比较了４种除菌方法的效果。结果表明,ＰＥＳＩ固体２基和ＭＳ固体在是诱导海带愈伤组织的理想培养基,其诱导率分别为７５．５％和６     

海带丝状雌配子体孤雌生殖的初步研究

比较了两种温度、两种光照强度、4种培养基对海带丝状雌配子体孤雌生殖的影响 ,实验结果表明 ,温度为 10 .0± 0 .5℃、光照强度为 30 0 0 lx、含铁氮磷营养海水培养基是适宜孤雌生殖的条件。在温度、光照强度和培养基 3种因素中 ,培养基组成是影响孤雌生殖速度和效率的最重要因素 ;通过比较找到了 3个孤雌生殖速度较快的丝状雌配子体品系。     

海带磷酸烯醇式丙酮酸-羟激酶的研究

于1986－1990年，在大连凌水养殖四场采集海带，对磷酸烯醇式丙酮酸羧激酶在海带不同部位、不同发育时期及不同外界条件下活性的变化予以研究。结果表明，该酶是海带体内唯一重要的催化CO2暗固定的酶；此酶活性由海带基部、中部至顶端逐渐减弱；酶活性在用正常水温预处理藻体时最高；正常盐度时酶的活性高于非正常盐度的酶活性；增加NH4+浓度可提高酶的活性。     

The effects of fucoidans from Laminaria japonica on AAPH mediated oxidation of human low-density lipoprotein

1Introduction Thegeneration,reactionandconsequenceof freeradicalsinbiologicalsystemshavereceived muchattentionrecentlyinconnectionwithavariety ofpathologicalevents(BarryandSusanna,1993;FangandLi,1999).LDLoxidizedbyexposureto tracemetalionsCu2 andFe3 (Hein…