日本七鳃鳗外周血细胞显微结构及类淋巴细胞体外培养

以日本七鳃鳗(Lampetra japonica)为研究对象,观察其外周血细胞显微结构和探讨类淋巴细胞原代培养条件。采用Wright氏和Giemsa氏染色法对日本七鳃鳗外周血液有形成分进行显微观察,可鉴别出红细胞、淋巴细胞、中性粒细胞、单核细胞、嗜酸性粒细胞;未发现嗜碱性粒细胞。通过Ficoll密度梯度离心法和流式细胞仪分选获得高纯度的七鳃鳗类淋巴细胞。经条件优化后确定最适培养液L15+20%FBS(5%七鳃鳗血清)+0.46%NaCl,在18℃,pH 6.8-7.0的条件下对日本七鳃鳗类淋巴细胞进行体外培养,大多数类淋巴细胞半贴壁生长,细胞状态较好,最长可以存活近一个月。类淋巴细胞的原代培养为日本七鳃鳗细胞体外培养的深入研究提供了实验依据。     

日本鳗鲡(Anguilla japonica)精养殖水体生物膜原位修复低碳养殖技术的研究

采用水体中设置弹性填料对比实验的方法,在12口聚乙烯水槽实施淡水精养殖日本鳗鲡,进行对鳗鲡精养殖水体生物膜原位修复节水增效效果、机理及应用前景的研究。结果表明,在207天的养殖期间,生物膜原位修复技术应用处理组的平均日换水率仅1.2%,比对照组显著节水减排74%(P0.01);处理组的主要水质因子pH、DO、TAN、NO2-N、NO3-N、COD等浓度均符合相关的渔业水质标准;处理组的起捕尾重、产量、生长速度分别比对照组高106%(P0.2)、108%(P0.2)和220%(P0.15),饲料系数低于对照组38.4%(P0.1)。此外,处理组弹性填料设置的适宜密度为填料占水体体积的10%。因此,精养殖水体生物膜原位修复技术,具有节水、减排与节能效果显著,投资与运行成本低,环保、低碳、安全,操作简便、易推广等优点。该革新性的生态工程低碳养殖技术对推动我国水产养殖发展方式从高耗费资源型向资源节约与环境友好型转变将产生重大影响。     

1987—2018年西双版纳橡胶林时空变化及其线状特征

基于1987—2018年西双版纳30 m分辨率橡胶林数据集,利用叠置、缓冲区等GIS空间分析方法,对比分析了过去32年橡胶林沿道路、河流和边境线的空间变化特征与区域差异。结果表明：1) 1987—2018年,西双版纳橡胶种植面积在道路、河流沿线5 km以内分别增加了1 874.6和1 484.6 km²,在边境地区(国境线20km缓冲带)增加了1 393.1 km²。2)从道路沿线看,受橡胶种植可达性和交通运输影响,过去32年道路5 km缓冲带内的橡胶林平均占比超过九成。3)从河流沿线看,因灌溉和水源等需求,过去32年河流沿线5 km缓冲带内橡胶林平均占比69.7%。值得注意的是,受天然橡胶价格持续低迷、城镇化与生态环境保护等影响,2010年河流沿线橡胶林种植面积开始下降,沿线2 km内表现尤为显著。4)从边境地区看,西双版纳橡胶林种植呈明显趋边性特征,跨境种植频繁。过去32年边境沿线地区橡胶林平均占比55.6%,且由1987年的35.4%上升到2018年的66.2%,其中,在中缅边境的扩张面积和速率高于中老边境。     

Are artificial neural network techniques relevant for the estimation of longitudinal dispersion coefficient in rivers? / Les techniques de réseaux de neurones artificiels sont-elles pertinentes pour estimer le coefficient de dispersion longitudinale en rivières?

Abstract

Abstract Accurate application of the longitudinal dispersion model requires that specially designed experimental studies are performed in the river reach under consideration. Such studies are usually very expensive, so in order to quantify the longitudinal dispersion coefficient, as an alternative approach, various researchers have proposed numerous empirical formulae based on hydraulic and morphometric characteristics. The results are presented of the application of artificial neural networks as a parameter estimation technique. Five different cases were considered with the network trained for different arrangements of input nodes, such as channel depth, channel width, cross-sectionally averaged water velocity, shear velocity and sinuosity index. In the case where the sinuosity index is included as an input node, the results turned out to be better than those presented by other authors.     

Towards developing sediment quality assessment guidelines for aquatic systems: An Australian perspective

Sediment at the sediment‐water interface of natural and man‐made waterways forms an integral part of the ecosystem because it is affected by a continuous flux of physical, chemical and biological components between the sediment, interstitial water and the overlying water column. Aquatic sediments contain records of past and present urban and rural runoff, chemical discharges and spills. In recent years sediment quality has received increasing attention following identification of the role of sediment as both a sink for pollutants and as a contaminant source with potential impacts on the quality of receiving waters. Research has indicated that the processes leading to remobilization of contaminated sediments in upstream reaches of a waterway may, through time, exert a significant influence on water quality in the downstream reaches. This, together with the cumulative effects due to contaminant input from point and non‐point source discharges, have dramatic effects on water quality and thus on ecosystem structure and functioning.

The problems associated with elevated concentrations of many hazardous organic and inorganic compounds have resulted in the establishment of aquatic sediment quality criteria and management guidelines in many overseas countries, with the objectives being the reduction and elimination of adverse environmental effects and human health risks associated with contaminated sediments. Whereas more than 70% of the Australian population is clustered around the coastal waterways, little is known about the role of sediments as a repository of environmental pollutants and/or as a source of adverse impacts on water quality and the health of our rivers. The paucity of knowledge on the quality of aquatic sediment highlights the need for the development of coherent guidelines for sediment quality assessment and management of contaminated sites, which are consistent with Australian environmental conditions and land use features.

A comparative evaluation of sediment quality information from eight coastal rivers along the east coast of Australia, presented in this paper, indicates the possibility for establishing a framework for regional sediment quality assessment. This may be achievable by using textural and compositional attributes of bottom sediments in depositional areas to develop databases on the loading and concentration trends of nutrients and contaminants. Regional variability in sediment quality determinants are shown to reflect the influence of catchment hydrology, lithology and land use on nutrient and contaminant concentration trends. Locally, the loading and partitioning behaviour of sediment‐bound contaminants is largely controlled by the nature and the extent of interactions occurring at the sediment‐water interface within individual depositional units.

The concept of ‘Sediment Effect Zone’ is introduced to provide a compartmental approach to the characterization of aquatic sediments and depositional environments in different hydrologic zones. This approach offers a rational basis for follow‐up chemical and biological assessments to establish sediment quality standards and management guidelines. Because of the complex influences of environmental, methodological and statistical factors on defining the sediment variability, the need for implementing proper quality control measures from early stages of design of a sediment quality assessment program is highlighted.     

三峡水库汛期控制水位及运用条件

随着长江上游梯级水库的陆续建成投运，三峡水库的水文情势和功能需求与设计条件相比发生了显著变化，仍维持固定的汛限水位运行已不能适应新形势需求。本文通过辨析三峡水库设计阶段汛限水位的设置条件，挖掘流域洪水特性和洪水遭遇规律，论证三峡水库汛期运行水位动态控制的可行性。结果表明：① 三峡水库设计推求的汛限水位145 m的适用条件是应对流域性大洪水，而流域性洪水发生概率小且特征明显，可以通过水文水情分析提前预判。② 根据流域洪水类型、洪水分期和遭遇规律，预判发生区域性大洪水时，三峡水库6月初至梅雨期结束汛限水位按145 m设置，从梅雨期结束后逐渐提高水位，8月20日后过渡到155 m。③ 在考虑上游水库群联合调度和气象水文预报的配合下，正常年份三峡水库汛期运行水位可在155 m上下浮动，并考虑提前蓄水。④ 三峡水库汛期运行水位动态控制，不会增加防洪风险和库区淤积风险，对中下游江湖关系和水文情势有利，可显著提高发电、航运、生态保护和供水等综合利用效益。     

Palaeozoic and early Mesozoic fish faunas of the Japanese Islands

Abstract In recent years, many fish teeth and scales have been found from the Palaeozoic and Mesozoic age strata of the Japanese Islands. This study is a compilation of the Japanese fish record from the Palaeozoic and early Mesozoic age deposits. Based on the published and unpublished data, the fossil fishes from the Palaeozoic and Early Mesozoic of Japan can be classified into 27 genera and 33 species, that is, one species of Devonian placoderms, 19 species of Permian to Jurassic elasmobranchs, three species of Permian cochliodonts, seven species of Carboniferous to Permian petalodonts, and three species of Triassic to Jurassic osteichthyans.     

水系分维的构造含义

本文运用分数维几何学,研究了河流水系分维与地壳里向运动、活动断裂格局及运动学特征的关联性。结果表明:1)地壳垂向运动控制大型河流主干的总体形态;2)活动断裂格局复杂、水平运动强烈的地区,水系分维值高;3)走滑断裂较斜滑断裂对水系发育的控制强烈,而后者主要起到对水系的分区作用。     

Slope-induced changes in channel character along a gravel-bed stream: The Allt Dubhaig,Scotland

Changes in channel character along a small river in the Scottish Highlands are described using measurements in seven reaches over a 3 km length with no significant tributaries but a decline in slope from 0.02 to 0.00015 because of local baselevel control. This decline in slope is associated with rapid downstream fining of the gravel bed followed by an abrupt transition to a sand bed. The channel pattern alters progressively rather than abruptly, in the sequence (1) near-braided, (2) meandering with active point-bar chutes, (3) meandering with active outer-bank talweg, (4) stable equiwidth sinuous. The changes in channel pattern and hydraulic geometry are predicted better by rational approaches based on critical shear stress or other physical concepts than by purely empirical discriminant or trend equations. Measurements in five reaches confirm a downstream decrease in shear stress and the amount and calibre of bedload. It is argued that the downstream changes in channel character in this stream are induced by profile concavity inherited from deglacial conditions, are typical of many streams in mountainous areas and can be understood in terms of slope-induced changes in hydraulic properties.     

云南三江一河典型地区河谷第四系发育特征

三江一河（金沙江、怒江、澜沧江、红河是流经云南境内的４条主体水系，将上述４条河流河谷第四系的野外观察加以总结得出；金沙江河谷局部发育早一中更新世的湖相层和河湖相层，常形成晚期阶地的基座，晚更新世至全新世冲积层构成四级阶地；怒江河谷第四系发育较差，在六库的上游河谷中冲积层极不发育，只有局部地方有少量的河漫滩砂砾层（全新世），在六库至过街河段中，尤其是过街盆地，发育有中更新世至全新世的冲积层和洪积层，前者构成五级阶地，后者形成巨大的洪积扇分布在河流右岸的谷底或苍坡上；澜沧江除景洪、勐罕（橄榄坝）盆地外，其它河段主要为峡谷，第四系很少，在景洪盆地中发育有第二、第一级阶地和河漫滩冲积层；红河的第四系有中更新世至全新世的冲积层，并构成五级阶地。基于上述第四系特点，简要地讨论了这４条河流的形成时代。