溴素的有机化学Ⅵ.多溴代邻羟基苯甲酸—2,3,5,6—四溴—1,4—苯二酯的合成

由邻羟基苯甲酸、１，４—苯二酚和溴素起始，经溴代、氧化—溴代、形成酰氯和酰化等步骤，合成了三种新的多溴代邻羟基苯甲酸—２，３，５，６—四溴—１，４—苯二酯。通过对产物溴含量、ＩＲ和１ＨＮＭＲ的分析，验证了产物的结构     

Surface geostrophic currents across the Antarctic circumpolar current in Drake Passage from 1992 to 2004

The Southern Ocean plays an important role in the global overturning circulation as a significant proportion of deep water is converted into intermediate and deeper water masses in this region. Recently, a secular trend has been reported in wind stress around the Southern Ocean and it is thought theoretically that the strength of the ACC is closely related to wind stress, so one consequence should be a corresponding increase in ACC transport and hence changes in the rate of the global overturning. There are no long-term data sets of ACC transport and so we must examine other data that may also respond to changing wind stress. Here we calculate surface currents in Drake Passage every seven days over 11.25 years from 1992 to 2004. We combine surface velocity anomalies calculated from satellite altimeter sea surface heights with measured surface currents. Since 1992, the UK has regularly occupied WOCE hydrographic section SR1b across the ACC in Drake Passage. From seven hydrographic sections surface currents are estimated by referencing relative geostrophic velocities from CTD sections with current measurements made by shipboard and lowered acoustic Doppler current profilers. Combining the seven estimates of surface currents with the altimeter data reduces bias in the estimates of average currents over time through Drake Passage and we show that surface current anomalies estimated by satellite and in situ observations are in good agreement. The strongest surface currents are found in the Subantarctic and Polar Fronts with average speeds of 50 cm/s and 35 cm/s, respectively and are inversely correlated, so that maximum westward flow in one corresponds to minimum westward flow in the other. The average cross-sectional weighted surface velocity from 1992 to 2004 is 16.7 ± 0.2 cm/s. A spectral analysis of the average surface current has only weakly increasing energy at higher frequencies and there is no dominant mode of variability. The standard deviation of the seven day currents is 0.68 cm/s and a running 12 month average has only a slightly smaller standard deviation of 0.52 ± 0.16 cm/s. The southern annular mode (SAM) measures the circumpolar average of wind stress and like the surface currents its spectrum has slightly increased energy at frequencies greater than 1 cpy. A cospectral analysis of these, averaging cospectra of five slightly overlapping 36 month segments improve statistical reliability, suggests that there is coherence between them at 1 cpy with the currents leading changes in the Southern annular mode. We conclude that the SAM and average Drake Passage surface currents are weakly correlated with no dominant co-varying modes, and hence predicting Southern Ocean transport variability from the SAM is not likely to give significant results and that secular trends in surface currents are likely to be masked by weekly and interannual variability.     

基于光谱阈值法和分裂合并聚类算法的“HY-1A”CCD云检测算法和应用

“海洋1号A”卫星(简称“HY-1A”)是我国第一颗海洋卫星,它搭载了水色扫描仪和CCD成像仪,其中CCD成像仪主要应用于海岸带地区.为了对海岸带地区进行准确的信息提取和分析,必须去除云的影响和干扰,因此本文针对“HY-1A”CCD数据的特点,对云检测算法进行了评估、遴选和改造,实现了业务化应用,这也是“HY-1A”卫星CCD成像仪海岸带应用示范系统的重要工作之一.云检测的方法众多,主要可以分为可见光/红外阈值法和聚类法两种,每种方法还可以辅以空间均匀性判识.     

Identification of aryl hydrocarbon receptor 2 in aquatic birds; cDNA cloning of AHR1 and AHR2 and characteristics of their amino acid sequences

The toxic effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and its related planar halogenated aromatic hydrocarbons (PHAHs) are mediated by the aryl hydrocarbon receptor (AHR). To investigate the potential sensitivity to PHAHs and the evolutional diversity of AHR in aquatic birds, AHR cDNAs were initially cloned and sequenced from the livers of a black-footed albatross (Diomedea nigripes) and a common cormorant (Phalacrocorax carbo). In this study, we report the identification of two distinct AHR paralog genes in these species. The two full-length AHR cDNAs from albatross were highly divergent (33% overall amino acid identity, and 60% identity in the N-terminal half). Phylogenetic analysis showed that one of them belongs to the AHR1 clade and the other one to the AHR2 clade, which has been identified only from fishes, but not yet from mammals and birds. Albatross AHR1 encoded a 861-residue protein with a predicted molecular mass of 96.7 kDa, and in the case of albatross AHR2, 925 amino acids and 100.7 kDa. From cormorant liver, the full-length AHR1 cDNA and the partial AHR2 cDNA were cloned. This result strongly suggests that bird species also possess two distinct AHR genes (AHR1 and AHR2). To our knowledge, this is the first report on the presence of an AHR2-like isoform in bird species as well as in fish.     

长江三角洲第一硬土层中微结核的矿物化学特征及其成因

长江三角洲第一硬土层是古土壤层,是长江河漫滩型沉积物,其形成经历多次沉积和成土过程.硬土层中的铁锰结核以菱铁矿、菱锰矿和褐铁矿为主,通过铁锰元素离子和矿物Eh-pH稳定关系分析,最有利于菱锰矿和菱铁矿结核生成的条件是:Eh＜0且pH＞8.成土期处于末次冰期低海面、气候寒冷干燥期,土壤的化学过程是以氧化作用为主,是褐铁矿等氧化性矿物形成时期;冰后期海相层的沉积覆盖使土壤层进入成岩阶段,土壤层的化学过程以还原作用为主,成岩过程是铁锰碳酸盐结核的主要生成期.     

The inventory and chemical characterization of dissolved proteins in oceanic waters

One-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and high resolution two-dimensional electrophoresis (2-DE) were applied to separate protein molecules in dissolved organic matter (DOM) from oceanic waters. Results were: (1) The 2-DE distinguished a total of 412 protein spots in 10 samples from five water columns over the Pacific, although fewer than 30 proteins were resolved as bands from the identical samples by SDS-PAGE. (2) Major and ubiquitous protein bands (34 and 39 kDa proteins) on the SDS-PAGE gel were resolved into horizontally spread arrays (trains) of spots on the 2-DE gels, indicating that these bands were a mixture of protein species that have the same molecular weight (MW) but different isoelectric points (pIs). (3) Proteins that exhibited such electrophoretic patterns on the 2-DE gels were glycosylated with variable linkages between the sugar and polypeptide chains. (4) N-terminal amino-acid sequencing demonstrated that individual spots within each train of spots had identical N-terminal amino-acid sequences.The N-terminal amino-acid sequences of the 39 and 34 kDa glycoprotein spots in samples collected at different sites were also identical. Protein isoforms with the same amino-acid sequence but different glycosylation profiles, termed glycoforms, were often observed on the 2-DE gel. Thirty-one and 24 spots on the 2-DE gels were glycoforms of two glycoproteins with MWs of 39 and 34 kDa, respectively; they were one protein species. The glycoforms of the 39 kDa protein were identified as a low molecular weight alkaline phosphatase (L-AP) of Pseudomonas aeruginosa PAO1 by a homology search through five amino-acid sequence databases. The present and earlier work indicates that all identified source organisms of dissolved proteins belong to the Pseudomonas group. We propose the hypothesis that proteins associated with membrane vesicles liberated from a minor member of the bacterioplankton assemblage, the marine Pseudomonas group, are one of the important sources of dissolved proteins in oceanic waters. This hypothesis may apply to the source pathway and survival not only of proteins and also to the universally occurring bacterial peptidoglycan and lipopolysaccharide components in DOM.     

冲绳海槽D－086孔颗石藻化石及其古海洋学意义的初步探讨

本文通过对冲绳海槽Ｄ－０８６孔颗石藻化石的研究，自下而上划分出三个颗石藻化石组合：Ｇｅｐｈｙｒｏｃａｐｓａａｐｅｒｔａ－Ｇ．ｏｃｅａｎｉｃａ组合，Ｇｅｐｈｙｒｏ－ｃａｐｓａｅｒｉｃｓｏｎｉｉ－Ｃｏｃｃｏｌｉｔｈｕｓｃｒａｓｓｉｐｏｎｓ组合，Ｅｍｉｌｉａｎｉａｈｕｘｌｅｙｉ－Ｇｅｐｈｙｒｏｃａｐｓａｏ－ｃｅａｎｉｃａ组合；划分出Ｅ．ｈｕｘｌｅｙｉ带和Ｅ．ｈｕｘｌｅｙｉ高峰带的界线；每一个颗石藻化石组合代表一个气候期，它们分别与标准气候期的玉木亚间冰期，晚玉木冰期和冰后期相当。     

有机质对S1nm锰矿相金属阳离子交换能力的影响

大洋多金属结核问题长期以来一直受到人们的关注[1～3].大量的研究结果表明,多金属结核中最有经济价值的铜、钴、镍等有价金属元素,主要赋存于仅占整个结核约15%锰矿相中.我们曾对人工合成1 nm锰矿相(synthetic 1nm manganate,以下简称S1nm锰矿相)金属阳离子交换能力作过初步探讨[4,5],研究表明,Cu2+,Co2+,Ni2+,Zn2+等与S1nm锰矿相具有强烈的阳离子交换能力,证明了多金属结核成矿后期的离子交换是铜、钴、镍、锌等有价金属元素进入多金属结核的重要途径之一.     

Monitoring the TOPEX and Jason-1 Microwave Radiometers with GPS and VLBI Wet Zenith Path Delays

Monitoring of altimeter microwave radiometer measurements is necessary in order to identify radiometer drifts or offsets that if uncorrected will introduce systematic errors into ocean height measurements. To examine TOPEX Microwave Radiometer (TMR) and Jason-1 Microwave Radiometer (JMR) behavior, we have used coincident wet zenith delay estimates from Very Long Baseline Interferometry (VLBI) and Global Positioning System (GPS) geodetic sites near altimeter ground tracks. We derived a TMR path delay drift rate of -1.1 ± 0.1 mm/yr using GPS data for the period from 1993.0-1999.0 and -1.2 ± 0.5 mm/yr using VLBI data. Thereafter, the drift appears to have leveled off. Already after 2.3 years (82 cycles) of the Jason-1 mission, it is clear that there have been significant systematic errors in the JMR path delay measurements. From comparison with GPS wet delays, there is an offset of -5.2 ± 0.6 mm at about cycle 30 and a more abrupt offset of -11.5 ± 0.8 mm at cycle 69. If we look at the behavior of the JMR coldest brightness temperatures, we see that the offsets near cycle 30 and cycle 69 are mainly caused by corresponding offsets in the 23.8 GHz channel of -0.49 ± 0.12 K and -1.18 ± 0.13 K, although there is a small 34.0 GHz offset at cycle 69 of 0.75 ± 0.22 K. Drifts in the 18.0 and 34.0 GHz channels produce a small path delay drift of 0.3 ± 0.5 mm/yr.     

The expression of CYP1A, vitellogenin and zona radiata proteins in Atlantic salmon (Salmo salar) after oral dosing with two commercial PBDE flame retardant mixtures: absence of short-term responses

The short-term effects of the commercial PBDE flame retardant mixtures Penta-BDE and Octa-BDE on the expression of cytochrome P450 1A (CYP1A), vitellogenin (Vtg) and zona radiata proteins (Zrp) were investigated in juvenile salmon (Salmo salar). For this purpose, groups of fish were dosed twice (oral intake at days 1 and 4) with 10 and 50 mg/kg body weight of both commercial mixtures. The fishes were sacrificed at day 7 (n=5 for each group) and 14 (n=6 for each group), and blood, liver, fillet, and brain were collected. Blanks and positive controls were also part of the experiment. The expressions of Vtg, Zrp, and CYP1A were measured with several techniques (EROD, ELISA, Western, Northern and Slot Blot). The values in the groups of fish treated with Penta-BDE or Octa-BDE did not significantly differ from the reference group for any of the parameters tested. In contrast, the positive control groups treated with estradiol-17β for Vtg and Zrp expression, and β-naphthoflavone for CYP1A expression did show a significant response, indicating the potential sensitivity of the fishes for the parameters measured. Since the results of the chemical analyses showed concentrations of a number of PBDE congeners in liver, fillet, and brain that were about three orders of magnitude above those of fish from the North Sea, it is concluded that the short-term toxicity of both commercial PBDE mixtures for these endpoints was low.