Isolation of protein from Chlorella sorokiniana CY1 using liquid biphasic flotation assisted with sonication through sugaring-out effect

Microalgae,a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical,bioenergy and food applications.This study aims to investigate the sugaring-out effect on the recovery of protein from wet green microalga,Chlorella sorokiniana CY1 which was assisted with sonication.A comparison of monosaccharides and disaccharides as one of the phaseforming constituents shows that the monosaccharides,glucose was the most suitable sugar in forming the phases with acetonitrile to enhance the production of protein(52% of protein).The protein productivity of microalgae was found to be significantly influenced by the volume ratio of both phases,as the yield of protein increased to 77%.The interval time between the sonication as well as the sonication modes were influencing the protein productivity as well.The optimum protein productivity was obtained with 10s of resting time in between sonication.Pulse mode of sonication was suitable to break down the cell wall of microalgae compared to continuous mode as a lower protein yield was obtained with the application of continuous mode.The optimum condition for protein extraction were found as followed:200g/L glucose as bottom phase with volume ratio of 1:1.25,10s of resting time for ultrasonication,5s of ultrasonication in pulse mode and 0.25g of biomass weight.The high yield of protein about 81% could be obtained from microalgae which demonstrates the potential of this source and expected to play an important role in the future.     

Growth inhibition and oxidative stress in two species of marine diatoms exposed to 1-phenylethanol

1-phenylethanol (1-PEA) is a flavor extensively used in the production of cosmetics, beverages, and food. The release of 1-PEA into coastal environments has aroused great concern. However, its potential effects on marine organisms are still unknown. In order to provide a better understanding of the ecological risks of 1-PEA in marine environments, this study determined the toxic effects of 1-PEA on two marine diatoms (Phaeodactylum tricornutum and Skeletonema costatum). The diatoms were grown in culture medium containing different concentrations of 1-PEA for 96 h. The contents of chlorophyll a, chlorophyll c, glutathione (GSH), malondialdehyde (MDA), and the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), were measured at the end of the exposure period. 1-PEA was shown to significantly inhibit the growth of diatoms, with 96-h EC50 values of 257.14 mg/Land 126.46 mg/L in P. tricornutum and S. costatum, respectively. In P. tricornutum, the levels of SOD, CAT, GPx, GSH, and MDA were stimulated only when 1-PEA concentrations were close to or greater than the 96-h EC50 value. However, in S. costatum, the activities of SOD and CAT, and the syntheses of two chlorophylls were inhibited even at an exposure concentration below the 96-h EC50 value. Taken together, these findings indicate a potential ecological risk by discharging 1-PEA into coastal areas and its species-specific toxic effects on marine organisms.     

Verification and recalibration of HY-2A microwave radiometer brightness temperature

HY-2A is the first one of the Chinese HY-2 ocean satellite series carrying a microwave radiometer(RM)to measure sea surface temperature,sea surface wind speed,atmospheric water vapor,cloud liquid water content,and rain rate.We verified the RM level 1B brightness temperature(T B)to retrieve environmental parameters.In the verification,TB that simulated using the ocean-atmosphere radiative transfer model(RTM)was used as a reference.The total bias and total standard deviation(SD)of the RM level 1B TB,with reference to the RTM simulation,ranged-20.6-4.38 K and 0.7-2.93 K,respectively.We found that both the total bias and the total SD depend on the frequency and polarization,although the values for ascending and descending passes are different.In addition,substantial seasonal variation of the bias was found at all channels.The verification results indicate the RM has some problems regarding calibration,e.g.,correction of antenna spillover and antenna physical emission,especially for the 18.7-GHz channel.Based on error analyses,a statistical recalibration algorithm was designed and recalibration was performed for the RM level 1B TB.Validation of the recalibrated TB indicated that the quality of the recalibrated RM level 1B TB was improved significantly.The bias of the recalibrated T B at all channels was reduced to<0.4 K,seasonal variation was almost eradicated,and SD was diminished(i.e.,the SD of the 18.7-GHz channel was reduced by more than 0.5K).     

"资源一号"02C卫星影像土地利用变化检测能力分析

“资源一号”02C卫星自成功发射以来,被广泛应用于土地资源、矿产资源、地质环境调查,以及国土资源、地质灾害应急监测等应用领域。“资源一号”02C卫星是一颗填补我国高分辨率遥感数据空白的卫星,是根据国土资源主体业务需求定制的第一颗国产高分辨率业务卫星。本文以吉林省和浙江省某试验区“资源一号”02C卫星数据为例,对“资源一号”02C卫星数据融合影像进行了土地利用遥感监测变化信息检测能力的方法试验。根据试验区数据变化信息提取试验结果,对“资源一号”02C卫星影像在土地利用动态遥感监测中的变化检测能力和适用性开展了试验、分析和评价工作。     

背负式移动激光扫描系统测绘大比例尺地形图精度试验研究

将背负式移动激光扫描系统应用在测绘大比例尺地形图中,其扫描精度至关重要。本文利用徕卡Pegasus Backpack对苏州工业园园区测绘地理信息大楼进行扫描,采用Inertial Explorer、Infinity、AoTumatic Processing对点云数据进行预处理,运用RealWorks提取特征点,将特征点在MicroStation V8联图中绘制成1：500地形图。通过与传统方法绘制的1：500地形图相叠合,发现两幅地形图具有很好重合度。对地物检测点精度分析后,得到点位中误差为0.026 m,高程中误差为0.041 m。研究结果表明徕卡Pegasus Backpack满足1：500地形图测量精度要求。     

Sentinel-1A SAR影像及其在矿区地面沉降监测中的应用研究

详细介绍了Sentinel-1A SAR影像数据的基本参数、工作模式、应用领域等。利用2016.11.09—2017.03.09的5景Sentinel-1A C波段SAR影像数据进行矿区地面沉降监测试验。基于SARscape利用双轨D-In SAR技术进行差分干涉处理,得到了研究区地面沉降分布图,直观地再现了研究区在2016.11.09—2017.03.09的沉降分布、沉降量级等。结果表明济宁某矿区在监测期间地面相对稳定,未有大面积和大量级的地面沉降发生,4个干涉对监测到的最大沉降都未超过3 cm。     

基于ETOPO1数据的全球及重点海域水深统计与分析

由于不同的水深探测手段及海深反演方法适用于不同深度的海域,且全球海域水深分布不均,因此在探测及反演海深之前,需对目标海域的水深分布有一定认识。本文采用ETOPO1模型对全球及部分海域进行统计分析,结果表明,全球及大西洋、印度洋、太平洋区域的水深大多处于3 000—10 000 m,其中3 000—5 000 m水深海域占比较大,因此,在这些海域主要采用重力数据结合船测数据进行反演,同时应注意避开海岸带、岛屿较多、海脊存在的区域;对于水深小于1 000 m的海域,可以根据外部环境,采用多波束船测、水深遥感、机载激光测深等方式进行探测。通过分析及统计全球和重点海域的水深可以为海深探测及海底地形反演方法的选取提供参考。     

基于Sentinel-1和D-InSAR的地表沉降地理国情监测技术研究

为推进合成孔径雷达差分干涉测量(D-InSAR,Differential Interferometric Synthetic Aperture Radar)技术在地表沉降地理国情监测中的应用,本文利用D-InSAR技术,以某矿区为研究对象,基于5景哨兵-1卫星(Sentinel-1)雷达影像,采用SARscape与Arc GIS软件相结合处理的方式得到了精准的成果数据,并结合实地水准观测结果对D-InSAR地表沉降监测的精度进行了对比分析。结果表明:利用D-InSAR技术进行地表沉降地理国情监测,具有较高的测量精度,且该技术具有大尺度连续覆盖能力、受天气干扰小、低成本等特点,在地理国情监测等相关领域有非常好的应用前景。     

基于Sentinel-1B SAR数据的洪水提取和监测

洪涝灾害是我国最严重的气象灾害之一,及时准确的洪灾监测是防灾减灾的重要前期工作和基础。本文利用sentinel-1B雷达数据,以黑瞎子岛为研究区,联合使用OSTU阈值分割法和随机森林面向对象分类法针对像素统计单波形、双波形、多波形SAR影像提取洪水要素,实现对洪灾淹没面积的时序监测,为灾情监测提供数据和技术支撑。     

灰关联GM(1,N)模型在建筑物沉降分析中的应用

针对建筑物沉降变形中各监测点相互关联、相互影响的情形,可将灰色系统理论中的GM(1,N)模型引入到建筑物沉降分析中。本文利用灰度关联方法确定相关因子的关联度,建立GM(1,N)模型,并与回归分析、GM(1,1)模型比较。通过工程实例,得出灰关联GM(1,N)模型预测精度明显高于另外两种模型,验证了该模型在进行建筑物沉降分析预测中应用的可行性。