基于ArcGIS建立地震救援现场地图符号库

针对地震救援这一特殊事件,按照一定的原则完成地震救援现场符号的分类,并对涉及的事物事件根据相应的原则完成符号设计。运用ArcGIS软件的2种符号库建立方法生成应用于地震救援现场的专题符号库,并对2种方法进行了简单比较。     

二维码定位技术支持下的室内导航电子地图的设计与实现——以武汉大学图书馆为例

随着大型复杂公共室内空间逐渐增多,室内导航已成为当前基于位置服务（LBS）领域的研究热点。本研究结合空间定位技术和移动地图技术,设计和开发了一种面向复杂室内空间的导航电子地图,利用二维码技术进行准确的室内空间定位,并以武汉大学图书馆为样区,设计了一套面向复杂室内空间结构的位置编码规则。在此基础上,开发和实现了可提供图书馆室内定位与最优路径推送的手机端导航电子地图。     

Construction of a muscle cDNA library of Chinese shrimp <Emphasis Type="Italic">Fenneropenaeus chinensis</Emphasis> and sequence analysis of the troponin I gene

A muscle cDNA library of Chinese shrimp (Fenneropenaeus chinensis) was constructed with the SMART™ cDNA Library Construction Kit. The titer of optimal primary library was 7.7×10⁵ pfu mL⁻¹ and that of the amplified library was 3.0×10⁹ pfu mL⁻¹. The percentages of the recombinant clones of primary and amplified libraries were over 98%. The insert sizes were longer than 400 bp with an average of 1000 bp. A positive clone containing a 794 bp insert was sequenced and identified encoding fast skeletal troponin I gene. This library provided a useful resource for the functional genomic research of F. chinensis.     

Construction of a Muscle cDNA Library of Chinese Shrimp Fenneropenaeus chinensis and Sequence Analysis of the Troponin Ⅰ Gene

A muscle cDNA library of Chinese shrimp (Fenneropenaeus chinensis) was constructed with the SMARTTM cDNA Li- brary Construction Kit. The titer of optimal primary library was 7.7×105 pfu mL-1 and that of the amplified library was 3.0×109 pfu mL-1. The percentages of the recombinant clones of primary and amplified libraries were over 98%. The insert sizes were longer than 400 bp with an average of 1000 bp. A positive clone containing a 794 bp insert was sequenced and identified encoding fast skeletal troponin Ⅰ gene. This library provided a useful resource for the functional genomic research ofE chinensis.     

地质调查技术方法信息网中物化探技术方法库内容建设

“物化探技术方法库”是“中国地质调查技术方法信息网”的有机组成部分。通过研究,将物探方法归纳为重力、磁法、电法、地震、放射性和地温6大类25种方法,将化探方法归纳为沉积物、土壤、岩石、气体、水、生物和其他等7大类16种方法。对每种技术方法均作了概要性的简介;经筛选,编制入库物化探技术方法、仪器设备、软件及应用实例等信息条目208条,初步充实了物化探技术方法库的内容,为服务平台物化探方法技术库运行提供了基本条件。     

Differential gene expression in the brain of Sebastiscus marmoratus in response to exposure to polychlorinated biphenyls (PCBs)

Effects of exposure to polychlorinated biphenyls (PCBs) on Sebastiscus marmoratus were investigated using a suppression subtractive hybridization method. A total of 108 gene sequences were identified as having the potential for being differentially expressed, and 45 could be identified with homologous database sequences. Functions with which they were associated included long-term potentiation and neurotransmitter release, neuroendocrine, mitosis and cell proliferation, energy-related metabolism, general metabolism, signal protein, hemopoiesis system, immune system, and structure. The expression of 17 of these genes was analyzed in the brain using real time fluorescent quantitative PCR. The present study provided a basis for studying the response of fish to PCB exposure and allowed the characterization of new potential neurotoxicol biomarkers of PCB contamination in seawater.     

Microbial diversity in polluted harbor sediments II: Sulfate-reducing bacterial community assessment using terminal restriction fragment length polymorphism and clone library of dsrAB gene

Sulfate-reducing bacteria (SRB) are important regulators of a variety of processes in coastal marine sediments regarding organic matter turnover, biodegradation of pollutants, and sulfur and carbon cycles. Yet their community compositions have not been investigated in polluted harbor sediments. This study described the diversity and spatial variation of SRB communities in surface sediments in Victoria Harbor, Hong Kong. The spatial variation of SRB communities was described by terminal restriction fragment length polymorphism (T-RFLP). The results showed that the most diversified terminal restriction fragments were found at polluted sites. In addition, cluster analysis indicated that although the SRB communities were different at the two polluted sites, they were still more similar to each other than to the two more distant reference sites. Based on a dsrAB clone library constructed at a polluted site, diversified SRB were found, represented by 30 Operational Taxonomic Units (OTUs). Upon comparisons among the SRB sequences detected from this study and those in the GenBank, five clades of SRB were found. Three clades belonged to the known families Desulfobacteraceae, Desulfobulbaceae, and Syntrophobacteriaceae. The majority of sequenced clones, which distantly related to sequences in the GenBank, constituted the remaining two unclassified groups, suggesting unique SRB members related to the polluted harbor environment. Statistical analyses indicated that estimated SRB richness correlated with environment factors such as sulfur content, acid volatile sulfate, and redox potential.     

知识经济与图书情报工作

知识经济是近年来世界范围内兴起的一个新概念。阐述了知识经济时代图情工作面临的机遇和挑战 ,同时阐述了图情工作在知识经济发展中的地位和作用。     

插件技术在天气雷达数据处理软件中的应用

介绍了在多普勒天气雷达二次产品软件的编写中使用动态链接库(Dynam—ic—link Library)技术设计的一个插件系统(Plug—in System)。该系统中所有的算法都设计成插件，将产品算法部分和系统本身分离，便于工程的管理和进一步开发，使研究人员能够集中精力致力于算法的实现。该系统本身也可以作为一个新算法、新产品的开发平台。     

Construction of a metagenomic DNA library of sponge symbionts and screening of antibacterial metabolites

To study the bioactive metabolites produced by sponge-derived uncultured symbionts, a metagenomic DNA library of the symbionts of sponge Gelliodes gracilis was constructed. The average size of DNA inserts in the library was 20 kb. This library was screened for antibiotic activity using paper disc assaying. Two clones displayed the antibacterial activity against Micrococcus tetragenus. The metabolites of these two clones were analyzed through HPLC. The result showed that their metabolites were quite different from those of the host E. coli DH5α and the host containing vector pHZ132. This study may present a new approach to exploring bioactive metabolites of sponge symbionts.