饲料中大豆异黄酮和大豆皂甙对牙鲆肝脏和肠道蛋白质消化酶活性和基因表达的影响

以鱼粉为蛋白源,小麦粉为糖源,鱼油为脂肪源配制基础饲料,分别添加不同浓度梯度(0.1%、0.4%和0.8%)的大豆异黄酮和大豆皂甙。以不添加大豆异黄酮和大豆皂甙的基础饲料作为对照组,共制成7种等氮(粗蛋白49.1%)、等能(总能20.1kJ/g)的实验饲料。在循环水系统中养殖初始体质量为(2.58±0.01)g的牙鲆(Paralichthys olivaceus)幼鱼,8周后观测牙鲆肝脏和肠道蛋白质消化酶活性和基因表达的影响。结果表明,牙鲆肠道胰蛋白酶的活性不受饲料中大豆异黄酮的显著影响(P>0.05),却随大豆皂甙添加量的升高而显著下降(P<0.05)。肠道糜蛋白酶的活性在饲料中大豆异黄酮添加量为0.1%时取得最大值(37.3±5.9)U/mg,显著高于其它处理组。饲料中添加不同水平的大豆异黄酮对肝脏中胰蛋白酶-1和糜蛋白酶-1基因表达量有显著(P<0.05)的影响,而添加大豆皂甙却对这些基因表达量的影响不显著。由此可见,大豆异黄酮直接作用于基因表达和酶活两个水平,而大豆皂甙只在酶活水平发挥显著的作用。同时,大豆异黄酮对牙鲆饲料利用率影响的双重性依赖于剂量的大小,其中的作用机制有待于进一步研究。     

福州沿海龟足的食性研究

龟足(Capitulum mitella Linnaeus)的食物组成可分为4 类, 动物性食物、植物性食物、有机碎屑和无机颗粒。动物性食物主要以桡足类为主, 植物性食物主要以硅藻类为主, 有机碎屑在龟足的食物组成中占据重要地位。龟足食物的食物多样性指数在夏季最高, 冬季最低。小规格龟足主要摄食植物性食物和有机碎屑, 未见摄食动物性食物; 大规格龟足和中等规格的龟足的食性差异不大, 以桡足类和有机碎屑为主。龟足的摄食强度在8 月最强, 1 月、2 月和12 月最弱。龟足摄取食物的大小范围很广, 能够摄取包括2～2 130 μm 大小的食物。     

Compensatory growth in hybrid tilapia (Oreochromis mossambicus×O. niloticus) reared in seawater, following restricted feeding

1 INTRODUCTION Compensatory growth or catch-up growth is a phase of unusual rapid growth induced by a period of environmental restriction; it had been demon-strated in a wide range of fish species following food deprivation (Bilton and Robins, 1973; Dobson and Holmes, 1984; Miglavs and Jobling, 1989; Quinton and Blake, 990; Wieser et al., 1992; Reim-ers et al., 1993; Kim and Lovell, 1995; Paul et al., 1995), restricted feeding (Weatherley and Gill, 1981; Jobling and Koskela, 1996; P…     

Measurements of Beam Pattern Perturbation in Corrugated Feed Horn Arrays for CMB Observations

An experimental study on the perturbation of the angular response of a W-band corrugated horn produced by nearby feeds, reproducing a typical situation of multi-feed arrays commonly used in high sensitivity Cosmic Microwave Background instruments, is reported in this paper. The effects for different positions of the scattering horn in the two principal polarisation planes were measured. We analyze the effects on both the side-lobe level and on the main beam. In particular, these results allowed us to validate design criterion for the multi-frequency focal array of the ESA PLANCK mission.This revised version was published online in July 2005. The first author’s e-mail address was removed.     

Ingestion of Domoic Acid and Its Impact on King Scallop（Pecten maximus,Linnaeus 1758）

A simple method for spiking formulated feed with domoic acid （DA） was developed in this study. DA feed was prepared by mixing 0.15 mL 100 μg mL-1 DA with 0.1 g formulated feed, and drying the mixture at room temperature for 2 h. The prepared DA feed contained 0.19 pg DA per particle. Of the added DA, 46.72% was retained in the feed. Relatively high DA retention （about 50%） was recorded after DA feed was soaked in water for 2h. Exposure to DA feed for 7d did not cause the increase of tissue DA level of adult king scallop （Pecten maximus） significantly in 60 d. The increase of their gonad index after DA exposure was not significantly different from the control. No significant change in DA level was found in spermary, ovary or fertilized eggs after DA exposure. These results indicated that DA excretion may be more efficient than DA accumulation under the current experimental conditions, and the mechanism of domoic acid incorporation in P. maximus may involve intracellular biotransformation.     

环境因子对L型、S型褶皱臂尾轮虫生长的影响

在实验室条件下,通过种群累积培养的方法,研究了不同盐度和小球藻(Chorellavulgaris)、亚心形扁藻(Platymonassubcordiformis)、微绿球藻(Nannochlorisoculata)、波吉卵囊藻(Oocystisborgei)等不同饵料微藻对L型褶皱臂尾轮虫(BrachionusPlicatilistipicus)和S型褶皱臂尾轮虫(BrachionusPlicatilisrotundiformis)生长的影响,探讨这两种轮虫在生态条件上的差异。结果表明:L型和S型褶皱臂尾轮虫对盐度的适应有显著的差异,生长的最适盐度分别为15和20,大水体培养时,其适宜生长的盐度范围分别是15~20和10 ~30。饵料微藻对L型和S型褶皱臂尾轮虫生长的影响一致。小球藻培养效果在4种饵料微藻中最好,其生长率明显高于微绿球藻和亚心形扁藻,波吉卵囊藻培养效果较差。     

导向强度实用计算方法与钻孔轨迹三维控制

通过分析影响导向钻进轨迹设计的主导因素,建立符合实际施工条件的导向强度计算公式,予以了试验和实际施工的验证,并藉此开发了三维轨迹设计系统。     

允饲植物源复方制剂对草鱼(Ctenopharyngodon idellus)组织形态和生长性能的作用研究

为了进一步评估由白芍(Radix paeoniae Alba)、杜仲(Eucommia ulmoides)和知母(Rhizoma anemarrhenae)组成且具有免疫增强作用的复方制剂A、B、C对草鱼(Ctenopharyngodon idellus)组织形态和生长性能方面可能存在的风险,本试验将二龄草鱼随机分成12个试验组和2个对照组,分别连续投喂含有不同复方制剂的饲料和空白饲料,在试验第7、14、21、28天分别随机从对应试验组中采集5尾草鱼肝胰脏、脾脏、肾脏和肠道进行切片制作和组织形态分析;在试验第0、29天分别随机从对应试验组中采集10尾草鱼进行肥满度、增重率、特定生长率、脏体比和肠道承重力测定。结果表明,分别以2%、4%剂量添加于草鱼饲料中的复方制剂A、B、C对试验草鱼阶段增重率、特定生长率、饵料系数、肝胰体比、肾体比和肠道承重力系列指标均无异常影响。组织形态分析方面,复方制剂A对草鱼肝胰脏、脾脏、肾脏和肠道具有良好的安全性,而复方制剂B、C经连续投喂21天起,对应剂量组草鱼肝胰脏、肾脏逐渐呈现不同程度的组织病变。本文得出结论认为,即使完全符合允饲要求的天然植物品种,缺乏基于较全面的评价维度试验证明其具有使用安全性,则容易仅关注允饲天然植物在效果表达的表象,而忽略其诱发的组织病变造成组织器官的功能衰退。     

Compensatory growth in hybrid tilapia (<Emphasis Type="Italic">Oreochromis mossambicus ×O. niloticus</Emphasis>) reared in seawater,following restricted feeding

Hybrid tilapia weighing 7.71 g were reared in seawater at 24.0–29.0°C for 8 weeks. The controls were fed to satiation twice a day throughout the experiment, whereas treatment groups were fed at 0.5%, 1.5% or 3.0% body weight per day for 4 weeks, and then to satiation for the remainder of the experiment. During the first 4-week period, there was a curvilinear relationship between growth rate and ration size. Fish fed 0.5% and 1.5% rations displayed compensatory growth response of 2 weeks duration during realimentation. The weight-adjusted growth rate of fish fed at 3% ration was not significantly different from that of the controls by the end of the experiment, when none of the treatment groups had caught up in body weight with the controls. Hyperphagia was observed for the first 2 weeks of realimenatation in fish previously fed at 3% ration, but persisted for the whole realimentation period in groups previously fed at 0.5% and 1.5% rations. None of the feed restricted groups showed improved digestibility, feed efficiency, or protein and energy retention efficiency. Supported by the State Key Laboratory of Freshwater Ecology and Biotechnology of China and Shanghai Fisheries University (Grant No. 200015).     

瞬变电磁球体参数求解公式的建立

利用两个不同半径重叠回线在球体上方的观测电位,经数学推导,得到求解球状地质体的中心埋深、半径和电阻率的计算公式,计算结果较稳定。