基于cox1条形码的鱼肚DNA分子鉴定

为鉴定鱼肚的正品来源,运用一对特异性引物扩增并测定10 个不同价格（700～1 500 元/kg）鱼肚样本的线粒体DNA 细胞色素c 氧化酶Ⅰ（cox1）约660 bp 的序列.通过BLASTN 比对DNA 序列同源度,判定鱼肚所属种类分别为鲈形目石首鱼科（Sciaenidae）、马鲅科（Eleutheronema）、笛鲷科（Lutjanidae）、带鱼科（Trichiuridae）.cox1 条码序列获取便捷,基于此条码序列的物种鉴定技术可用于鱼肚种类鉴别.     

Effects of substratum type on fish assemblages in shallow areas of a tropical estuary

While there is already a comprehensive understanding of the effects of environmental variables, such as dissolved oxygen, temperature and salinity, on the structure, biomass and metabolism of aquatic biota in estuarine habitats, the effect of sedimentation, a harmful underlying factor, remains unclear. The aim of this study was to assess the differences in fish assemblages along the freshwater to salt water gradient of a large tropical estuary, and to evaluate the effects on them of habitat disturbance associated with shallow water sedimentation in the intertidal areas. Fish and environmental variables were recorded in the upper, middle and lower salinity zones of the estuary at ebb tide in both the dry and wet seasons. Sediment samples associated with different levels of habitat disturbance were analysed using granulometry, and their organic content and dissolved oxygen levels were quantified. Water temperature, salinity, pH and dissolved oxygen levels were also measured. Habitat disturbance was found to be correlated with the density, biomass and richness of fish assemblages. A total of 77 species were recorded, forming two distinct fish assemblages, with the Eleotridae family dominating in the upper zone, and Gerreidae, Gobiidae and Tetraodontidae the most common in the middle and lower estuary. Changes in the structure of fish assemblages, including reductions in density, biomass and richness, were associated with disturbance to natural features, where muddy sediment was replaced by sandy sediment and the quantity of organic matter fell. Atherinella brasiliensis was the species which showed a preference for the disturbed areas in the middle and lower zones, while Dormitator maculatus showed a preference for them in the upper estuary. They may be taken as indicators of habitat disturbance due to shallow sedimentation.     

利用人工鱼群算法优化高斯过程模型及应用分析

基于高斯过程利用传统共轭梯度法搜索超参数,存在对初始值依赖性强、获得局部最优解的不足。本文采用人工鱼群算法对超参数进行智能寻优,建立了基于人工鱼群算法的高斯过程模型对变形体形变进行预测分析。通过隧道和基坑两个工程实例计算比对分析,NN、SE和RQ 3种核函数中NN核函数的预测效果最好,平均相对误差分别为0.69%和1.06%。结果表明超参数优化模型的预测精度得到了较大的提高,改善了高斯过程算法本身存在的超参数求解方面的不足,效果明显。     

广东省应大力发展海洋牧场

综述了我国及广东省十多年来鱼虾种苗人工放流及人工鱼礁的投放情况，结合广东省广大海区的水温、盐度、ｐＨ值、海流、营养盐含量情况及天然饵料的种类、分布等，提出了除在大亚湾已设置了水产增殖站以外，还应该在广东东部的南澳和西部的湛江各设一个增殖站，会收到更大的效果。人工鱼礁应总结过去投放的经验，加强礁区管理，逐步解决投资与收益的关系问题，并进一步扩大投放人工鱼礁的地点和密度，把广东海区切实建成理想的海洋牧场。     

Primary fish survey in the Huanghe River estuary

The survey in the Huanghe River estuary indicated that the 85 fish species found there comprised three faunal elements: warm-water species (29.4%), warm-temperate species (55.3%) and cold-temperate species (15.3%). The seasonal changes of fish species, density and biomass are obvious. The total biomass is highest (7 699t) in August and lowest (489 t) in January.Setipinna taty has mean density of 9 278 indiv/km², and is the most important species constituting 25.5% of the total weight of fish caught.Trichiurus haumela was a dominant species in the 1950s, but no one was caught in this survey. There were obvious changes of the dominant fish species during the recent three decades. The demersal fish biomass has dropped greatly to only 1/10 in over 50 years since 1930. Contribution No. 2210 from the Institute of Oceanology, Academia Sinica.     

Advances in the Study on Nutrient Requirements of Grouper（Epinephelus sp. ）：a Review

The paper reviews the recent advances in studying grouper nutrition requirement for the development of cost-effective and environmentally friendly artificial diets. It consists of seven parts: protein and amino acid, lipid and essential fatty acid, carbohydrate, vitamin, mineral, alternative protein source, broodstock and larval nutrition. The review provides some basic information for further investigation of nutrient requirements of groupers.     

Flounders follow the tides to feed: Evidence from ultrasonic tracking in an estuary

Ultrasonically tagged flounders were tracked in the estuary of the River Bann, Northern Ireland, for periods of between 2 and 12 h (11 fish for a total of 92 h and 2·5 km), during their summer residence. Positions were determined by triangulation with two receivers.All fish remained within 400 m of the release point; on average flounders moved about 270 m during one tidal cycle, equivalent to 830 body lengths, or just over 1 body length per minute. Movements were most likely at dusk, but other parameters were not contingent upon time of day, although no data were gathered at dawn. Frequency, direction, distance, speed and duration of movement and turn rate were all significantly contingent upon state of the tide. The movement data are interpreted in terms of feeding behaviour, supported by results from other parts of the study. At high tide flounders made active but meandering searches for food on the inundated mudflats. Similar but faster searches heading downstream on falling tides reflected (a) completion of feeding while littoral invertebrates were still available and (b) avoidance of stranding. Flounders made infrequent but long and linear passages downstream at low tide, when they tended to bury themselves at the edge of the main channel where there was little food. Subsequently, the fish followed the rising tide upstream so that the cycle of movements kept most fish in the same area of the estuary in the short term. It is suggested that tolerance of changing salinities enables flounders, more than other flatfish species, to exploit dense populations of estuarine intertidal invertebrates as food.     

Comparison of the acid-base responses to CO2 and acidification in Japanese flounder (Paralichthys olivaceus)

To investigate whether the biological toxicity of aquatic hypercapnia is due to the direct effects of CO2 or to the effects of acidification of seawater by CO2, the Japanese flounder (Paralichthys olivaceus) was subjected to seawater equilibrated with a gas mixture of air containing 5% CO2 (pH 6.18) or seawater acidified to the same pH with 1 N H2SO4. All the fish died within 72 h in the CO2 exposure group, whereas no mortality occurred in the acid group. Acid-base parameters as well as plasma ion concentrations were severely perturbed in the CO2 exposure group, whereas they were minimally affected in the acid group. These results clearly demonstrate that the mortality in the CO2 group is a direct result of the elevated levels of dissolved CO2 and not to the effects of the reduced water pH.     

莫莫格国家级自然保护区的鱼类多样性

2008～2010年,在鱼类资源调查的基础上,分析了莫莫格国家级自然保护区的鱼类多样性特征。调查结果显示,莫莫格国家级自然保护区有鱼类物种5目11科42属49种,其中本区土著种5目10科38属45种,包括中国特有种4种,中国易危种5种,冷水种8种,国家II级保护动物1种。土著种群落中,有鲤形目(Cyprini-formes)鱼类32种,鲤科(Cyprinidae)鱼类29种,鮈亚科(Gobioninae)鱼类13种;古北界北方区黑龙江亚区黑龙江分区的物种为22种;源于东部平原和北方平原生态类群的鱼类有27种;适应于江—湖缓流环境和淡水定居生活的鱼类有33种;草上产卵和水层产卵繁殖的鱼类有37种;杂食性和初级肉食性种类有28种,它们均相对占优势。莫莫格国家级自然保护区与嫩江的鱼类群落物种结构Jaccard系数和Morisita-Horn相似性指数分别为0.918和0.838;稀有种(包括中国易危种)和常见种分别为14种和25种;物种多度格局近似于对数正态分布。这些结果表明,莫莫格国家级自然保护区的鱼类多样性与嫩江密切相关,鱼类区系复杂,物种组成与生态类型多样;群落结构相对较稳定,多样性程度相对较高,但仍面临着稀有种(包括中国易危种)尚占有一定比例而导致群落结构脆弱的潜在威胁。保持嫩江鱼类群落结构的稳定,对维护和发展莫莫格国家级自然保护区的鱼类多样性具有特别重要的意义。