An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified
and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing
four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene.
Supported by High Technology Research and Development Program of China (863 Program, No. 2006AA100309) 相似文献
通过对灰色GM[1,1]模型的算法改进,将改进后的灰色模型与马尔科夫链结合,既可以发挥灰色系统预测精确的特点,又可以利用马尔科夫链对准确预测波动性数据的优势。在灰色马尔科夫链模型的算法基础上,采用Visual Studio 2005开发环境,进行灰色马尔科夫链预测系统的设计。最后利用南方某地区十年来土地利用数据进行系统验证,结果表明,灰色马尔科夫链模型能很大地提高预测的精度和效果,符合实际要求。 相似文献
An explosive extratropical cyclone(EC)over the Eastern Asian region that caused two shipwrecks is analyzed using ERA-Interim reanalysis data from the European Center for Medium-Range Weather Forecasts.Analyses of the evolution of the EC reveal that the positive potential vorticity(PV)at the upper-tropospheric level displays a hook-shaped structure during the mature period of the cyclone.The PV distribution forms a vertically coherent PV structure called a PV tower.The vertical distribution of the PV can induce and strengthen cyclonic circulation from the lower-to upper-levels of troposphere,which is an important deepening mechanism of explosive cyclone.The PV tower occurs approximately ten hours prior to the development of surface occlusion in the cyclone.The evolution of surface fronts closely follows the development of the horizontal upper-tropospheric PV.This tandem development is largely attributed to the ability of the positive upper-tropospheric PV and the PV tower to induce cyclonic circulation simultaneously.The kinematic wrap-up process of cyclonic circulation also accelerates the formation of warm occlusion.A conceptual model of the distributions of positive PV and potential temperature combining the perspectives of dynamic tropopause folding,PV tower,and atmospheric stability,including westward tilting and baroclinicity,is proposed.This model can illustrate the explosive deepening mechanism of ECs.The regions of convective instability and rainfall determined by this model are consistent with those identified from the actual observation. 相似文献
Observations have been conducted using the Ooty Radio Telescope in order to place constraints on the evolutionary scenario
leading to the formation of the present day superclusters. The experiment attempted to detect 21 cm emission from massive
neutral hydrogen condensates at a redshift ofz = 3.3. In an Einstein de-Sitter universe with baryon density Ω = 0.05, about ten condensates were expected in the volume
surveyed if superclusters, having H I masses ≃5 × 1015M⊙, were the first objects to separate out of the Hubble expansion. The sensitivity of our experiment rules out the existence
of these condensates atz = 3.3 unless their lifetimes are less than one-tenth the dispersion in their epoch of formation or the proto-superclusters
subtend angles greater than 6 arcmin. The result indicates that superclusters form at z > 3.3 if indeed they were the first
objects to condense out of the Hubble flow.
An erratum to this article is available at . 相似文献