Establishment of a spleen cell line from large yellow croaker Pseudosciaena crocea and its primitive application in foreign gene transfection

A large yellow croaker,Pseudosciaena crocea,spleen(LYCS)cell line was established and the feasibility of using it for foreign gene transfection was evaluaed in this study.Primary culture of LYCS cells was initiated from spleen tissue pieces,which were cultured at 25℃ in Dulbecco’s modiced Eagle medium/F12 medium(DMEM/F12,1:1)(pH7.2),supplemented with 20% fetal bovine serum,carboxymethyl chitosan,chondroitin sulfate,basic fibroblast growth factor(bFGF)and insulin-like growth factor-I(IGF-I).The cultured LYCS cells,in fibroblast shape,proliferated to 100% confluency 20 days later.Chromosome analyses indicated that the LYCS cells exhibited chromosomal aneuploidy with a modal chromosome number of 48 which displayed the normal diploid karyotype of P.crocea(6m+6sm+36t,NF=60).A LYCS cell line,with a population doubling time of 48.7 h at passage 60,has been established and subcultured to passage 70.Transgenic feasibility test demonstrated that positive green fluorescence protein(GFP)expression was observed in LYCS cells after pcDNA3.1-GFP plasmid transfection.In conclusion,a continuous foreign gene trans-fection feasible LYCS cell line has been established successfully.The cell line might serve as a valuable tool for studies of transgenic breeding and has potential applications for different kinds of cytotechnological studies.     

Marine Yeasts and Their Applications in Mariculture

1Introduction Becauseyeastscanproducemanybioactivesubstan ces,suchasprotein,aminoacids,vitamin,polysac charide,fattyacid,phospholipid,polyamine,astax anthin,βcarotenoid,trehalose,glutathione,superox idedismutase,chitinase,amylase,phytase,protease,killert…     

Hydrolysis Activities of the Particle of Agarose-Ce^4＋ Complex for Compounds Containing Phosphodiester or Peptide Bonds

Hydrolysis activities of PACC （particle of agarose-Ce^4＋ complex, newly made through double emulsification） for compounds containing phosphodiester or peptide bonds were studied. The results showed that PACC could hydrolyze organophosphorous pesticides not only in water but also in vegetable juice or tea extract. Hydrolysis rates of methamidophos, omethoateand chlorpyrifos in water are 32.39%, 27.12% and 46.62% respectively, those of chlorpyrifos and methamidophos in mung sprout juice 38.28% and 35.45% respectively, and that of chlorpyrifos in tea extract 59.76%. Hydrolysis rates of BSA （bovine serum albumin） in water and protein in tea extract by PACC increase by 54.30% and 86.46% respectively as compared with the control.     

介绍GPS定位中的几个概念

文章概略介绍了利用卫星进行定位的原理 ;由卫星信号传播、信号接收和SA政策等引起的理论误差 ;给出了多种快速、高精度定位的工作模式和观测点选择的原则 ;列出了将WGS - 84坐标转换为我国坐标的公式及解算参数的具体方法 ,供读者在GPS工作中参考。     

绿松石呈色的晶体场理论计算

绿松石中Cu^2 电子组态为3d^9,为中心对称配位八面体，配位体为4个OH^-和2个H2O，在晶体场作用下，五重简并的d轨道分裂为5个能量不同的轨道，dx^2-y^2为基态，其余为激发态，依跃迁选律．从基态到四个激发态的跃迁为自旋允许跃迁，从而形成了铜离子吸收光谱的4条谱带。前人对绿松石中Cu^2 晶体场势能计算时，个别系数有误，笔者进行了修改，并补充了部分角度函数的积分值，从而使结果得到了改进，计算谱带位置与实测可见光吸收光谱谱带位置十分接近。在进行晶体场势能计算时，角度函数积分值是非常重要的，以往的资料只是直接给出了部分积分值，这在计算低对称配位八面体晶体场势能时是不够的，这次重新计算了所有可能的角度函数积分值，对各种对称下配位八面体晶体场能量的计算提供了可能。     

A BOOTSTRAP RESAMPLING SCHEME FOR USING THE CANONICAL CORRELATION TECHNIQUE IN RANK ESTIMATION

Rank estimation by canonical correlation analysis in multivariate statistics has been proposed as analternative approach for estimating the number of components in a multicomponent mixture.Amethodological turning point of this new approach is that it focuses on the difference in structure ratherthan in magnitude in characterizing the difference between the signal and the noise.This structuraldifference is quantified through the analysis of canonical correlation,which is a well-established datareduction technique in multivariate statistics.Unfortunately,there is a price to be paid for having thisstructural difference:at least two replicate data matrices are needed to carry out the analysis.In this paper we continue to explore the potential and to extend the scope of the canonical correlationtechnique.In particular,we propose a bootstrap resampling method which makes it possible to performthe canonical correlation analysis on a single data matrix.Since a robust estimator is introduced to makeinference about the rank,the procedure may be applied to a wide range of data without any restrictionon the noise distribution.Results from real as well as simulated mixture samples indicate that when usedin conjunction with this resampling method,canonical correlation analysis of a single data matrix isequally efficient as of replicate data matrices.     

用于大地网参数平差中计算权系数矩阵的递推公式

本文导出了大地网参数平差中计算权系数矩阵的递推公式,并通过实例说明它在经典平差和自由网伪逆平差中的应用。由于这一方法无需组成法方程组,所以可作为无法方程组的参数平差法的计算基础。 由于本方法具有很高的精度,又比传统方法简便,所以它在平差计算中将有广泛的应用前景。     

无界单元在地下水流计算中的应用

在人为选定的模拟无限边界的有限位置处设置无界单元。在该单元上配置带有衰减特征的线性插值函数,进而可建立按有限元法或不规则网格有限差分法解算地下水流问题所需要的单元刚度矩阵。该单元刚度矩阵可很容易地进入总刚度矩阵。无界单元的应用较好地解决了地下水流数值模拟中无限边界不易确定的问题。计算实例表明本方法具有较高的精度。     

空间权重矩阵的生成方法分析与实验

区域空间动力学过程 (例如疾病传播、区域社会经济可持续发展等 )所涉及的数据样本和状态变量都是以区域多边形形式存在的。区域群发展在空间上的此起彼伏和相互影响则是通过区域之间相互联系得以实现的 ,空间权重矩阵用以传载这一作用过程。因此 ,构建区域空间连接权重矩阵是建立区域空间动力学方程的核心问题之一。本文分析了空间权重矩阵的不同生成方法 ,并对空间权重矩阵的一种形式.     

二端口网络理论在振荡电路分析中的应用

本文将二端口网络理论应用于三点式振荡电路分析,得出了振荡频率较完整的数学表达式,以及振荡条件与器件参数之间的关系,从而为设计该类振荡器提供更加准确的计算依据。