基于cox1条形码的鱼肚DNA分子鉴定

为鉴定鱼肚的正品来源,运用一对特异性引物扩增并测定10 个不同价格（700～1 500 元/kg）鱼肚样本的线粒体DNA 细胞色素c 氧化酶Ⅰ（cox1）约660 bp 的序列.通过BLASTN 比对DNA 序列同源度,判定鱼肚所属种类分别为鲈形目石首鱼科（Sciaenidae）、马鲅科（Eleutheronema）、笛鲷科（Lutjanidae）、带鱼科（Trichiuridae）.cox1 条码序列获取便捷,基于此条码序列的物种鉴定技术可用于鱼肚种类鉴别.     

���ʽ���̽��ƻ���չ���ҹ����������ݶȼƻ���ʵʩ

????????????????????ù????????????????????????????????????????????????????????????????????SST-HL/SGG-Doppler-VLBI????????????????????50~100 km??     

Limited phylogeographic structure for five bathyal ophiuroids at continental scales

There have been comparatively few large-scale studies on spatial genetic structure of bathyal seafloor fauna, despite the importance of these data to the successful management of the world's oceans. We use a comparative analysis of mitochondrial DNA from five bathyal (200–3500 m) species of brittle-stars (Ophiuroidea) to assess phylogeographic structure along an extensive (8000 km) longitudinal gradient at temperate latitudes (28–56°S) from south-west Australia (113°E) to seamounts east of New Zealand (175°W). We found no evidence of a genetic discontinuity between Australia and New Zealand, either across the temperate Tasman Sea or across the Southern Ocean between the South Tasman Rise and the Macquarie Ridge. However, there were latitudinal phylogeographical breaks between tropical, temperate and polar regions; longitudinal breaks across the eastern Indian Ocean; and a bathymetric break at approximately 1700 m. Although there was limited regional structure in the frequency of haplotype distributions within the major clades, and no clade appeared to be strictly panmictic, the regional structure in general was not concordant with a simple isolation-by-distance model. Demographic structure varied with three clades having a simplified haplotype network, low effective population sizes and no evidence of significant population expansion, and two clades having a high diversity of haplotypes, relatively high effective population sizes and signs of recent population expansion. These results are discussed with respect to putative dispersal strategies. We hypothesise that the ‘brooding’ species produce both brooded young and pelagic larvae, allowing for both the maintenance of local populations and long-distance dispersal.     

Homogeneity testing of microanalytical reference materials by electron probe microanalysis (EPMA)

Homogeneity testing of candidate reference materials requires distinguishing the effects of measurement uncertainty of the analytical method from true compositional variations within the material. Many in situ microanalytical techniques do not allow classical ANOVA homogeneity testing due to the infeasibility of truly replicated analyses on the same analysis volume. This also applies to the analysis of beam-sensitive and light element-bearing materials by electron probe microanalysis (EPMA). This reality has led me to reconsider the homogeneity index approach used in the testing of microanalytical reference materials by EPMA. Based on statistical considerations, I show that the homogeneity index is suitable for statistical significance testing using F and chi-squared statistics and allows estimating the contribution of compositional heterogeneity to the total uncertainty budget of the referenced values. However, there are problems of bias and masking of small compositional variations by measurement uncertainty. This contribution shows the strong impact of the total number of measurements on the resolution of a microanalytical homogeneity study and discusses how to quantify the relative contribution of heterogeneity to the total uncertainty budget. I present an example of EPMA to illustrate this approach and show some pitfalls and limitations in its application.     

Analysis of coprolites from the extinct mountain goat Myotragus balearicus

Humans colonized the Balearic Islands 5–4 ka ago. They arrived in a uniquely adapted ecosystem with the Balearic mountain goat Myotragus balearicus (Bovidae, Antilopinae, Caprini) as the only large mammal. This mammal went extinct rapidly after human arrival. Several hypotheses have been proposed to explain the extinction of M. balearicus. For the present study ancient DNA analysis (Sanger sequencing, Roche-454, Ion Torrent), and pollen and macrofossil analyses were performed on preserved coprolites from M. balearicus, providing information on its diet and paleo-environment. The information retrieved shows that M. balearicus was heavily dependent on the Balearic box species Buxus balearica during at least part of the year, and that it was most probably a browser. Hindcast ecological niche modelling of B. balearica shows that local distribution of this plant species was affected by climate changes. This suggests that the extinction of M. balearicus can be related to the decline and regional extinction of a plant species that formed a major component of its diet. The vegetation change is thought to be caused by increased aridity occurring throughout the Mediterranean. Previous hypotheses relating the extinction of M. balearicus directly to the arrival of humans on the islands must therefore be adjusted.     

Fast tidal cycling and the origin of life

Replicating prebiotic polymers are thought to predate the emergence of true life-forms. The initial mode of replication, a prerequisite for Darwinian selection, is unknown, but demands an explanation based on local physicochemistry. Dual consideration of the conditions of the early terrestrial surface, with the unusual physicochemical properties of nucleic acids like DNA, could explain the emergence of nucleic acids as key biomolecules. The early impact that produced the Moon, and fast terrestrial rotation, subjected coastal areas 3.9 Ga ago to rapid tidal flooding (dilution) and drying (concentration), with a likely periodicity in the range of 2-6 h, and could have provided a driving force for cyclic replication of early biomolecules. Such a mechanism applies only to molecules capable of association/polymerization at high salt concentration, and of dissociation at low salinity. Nucleic acids meet these criteria. It is suggested that tidal cycling, resembling the polymerase chain reaction (PCR) mechanism, could only replicate and amplify DNA-like polymers. This mechanism suggests constraints on the evolution of extra-terrestrial life.     

The genotoxic effects of benzo[a]pyrene and methamidophos on black porgy evaluated by comet assay

In this study, two common pollutants （benzo[a]pyrene and methamidophos） in marine environment were tested by comet assay for their inducement of in vivo genotoxic effect to the blood cells of black porgy （ Acanthopagrus schlegeli）. The fish was exposed to 2 μg/L of benzo[a]pyrene （BaP） and methamidophos, and their mixture. The assay was performed on whole blood at 2 h, 5 h, 24 h and 96 h exposure intervals. A significant increase in DNA damage was observed in each treatment with the pollutants. Additive effect of BaP and methamidophos was also found in the experiment. However, the decrease ratios of DNA damage for 5 h and 96 h exposure interals compared with 2 h and 24 h exposure ones, respectively, were noticed. This phenomenon may be explained by the function of repairing process via enzyme cytochrome P450 in the animal. Evidence of the genotoxicity of organophosphorus pesticides （OPs） and polynuclear aromatic hydrocarbons （PAHs） on marine fish are discussed in this paper.     

大别山地区沙坪沟斑岩型钼矿床蚀变及矿化特征研究

沙坪沟钼矿床是大别山地区新近发现的、世界第二大的斑岩型钼矿床。本文在前期以及前人的工作基础上,通过详细的野外地质观察和系统的岩相学、矿相学以及探针测试分析工作,详细研究了沙坪沟钼矿床的蚀变与矿化特征。结果表明,沙坪沟钼矿床的围岩蚀变类型主要有钾硅酸盐化、青磐岩化和绢英岩化,绢英岩化又可细分为以石英为主和以绢云母为主。矿床中产出16种脉体类型,根据矿床的脉体类型划分了4个成矿阶段:(1)石英-钾长石阶段;(2)石英-硫化物阶段;(3)石英-绢云母阶段;(4)石英-萤石-石膏阶段。蚀变及矿化特征显示,沙坪沟钼矿床的钼矿化开始于石英-钾长石阶段晚期,结束于石英-绢云母阶段早期,石英-硫化物阶段是辉钼矿主要的形成阶段。沙坪沟钼矿床经历了多期次脉动式成矿流体的蚀变与矿化过程,在不同蚀变-矿化阶段中,成矿流体的物化条件和组分的变化是控制各阶段蚀变类型和脉体中矿物组合的主要因素。产于以伸展为主的板内环境的沙坪沟钼矿床与其他构造环境下的斑岩钼矿床在与成矿关系密切的岩石、蚀变分带等方面相似,但由于围岩性质的差异,矿体赋存位置及产状不同;而在矿物种类、矿化产出位置及产状、与矿化关系最为紧密的蚀变类型等方面存在差异。斑岩型钼矿床的构造背景可能控制了其岩浆的形成、演化以及含矿性,而岩浆岩最终定位的深度、围岩等条件也是控制蚀变和矿化特征的重要因素。     

RAPD分析中最适样本量和位点数的研究

用25个引物,对40个样本扩增,研究了勒氏笛鲷随机扩增多态性DNA(RAPD)分析中的最适样本量和位点数。结果显示:在样本量达到20且位点数达到70个以上时,遗传距离趋于一个稳定的数值,即在RAPD分析中,样本数应达到20且位点数应达到70才能保证结果的可靠性。     

双链DNA片段的碱基空间模式BSP——基因组调控区的一种可能的信息编码方式

生物体可看作是一种特殊的信息处理系统 ,论文的第二章从这种观点出发讨论了生物细胞内最大的信息源基因组DNA序列的信息编码问题。首先探讨了基因组蛋白质编码区的信息编码问题 ;简要回顾转录水平的基因表达调控后 ,讨论了基因组基因表达调控区的信息编码问题。第三章介绍了我们利用internet上可获取的开放的生物信息学资源 ,对基因组DNA调控区的一种可能的编码方式所进行的理论研究。这种可能的编码方式我们称之为双链DNA上的碱基空间模式BSP (BaseSpatialPattern)。首先 ,我们发现了由三联体BSP介导的一种 4与 2 0的关系 ,称为 4 -BSP -2 0关系。并推测 4 -BSP -2 0关系也许是某些DNA—蛋白质相互作用中的一种识别密码 ;第二 ,研究真核细胞转录调控中的DNA—蛋白质相互作用的实验数据表明 :某些DNA结合蛋白质对双螺旋核酸的专一性识别可能与BSP有关。并找到了 7组由其他生物学家公开发表的实验数据支持这一猜测 ;第三 ,研究了BSP的形式化描述问题 ,找到了一种基于等价类的BSP描述方法 ,并开发了相应的计算机处理算法。该描述方法简明且便于用计算机进行处理 ,更重要的是它提供了一种研究途径 ,可把BSP和 4 -BSP -2 0关系推广到单链DNA (ssDNA)和ssDNA -蛋白质相互作用的研究中 ,从而可进一步推广到RN