EFFECTS OF LOW MOLECULAR WEIGHT CHITOSAN (LMC-1) ON SHRIMP PRESERVATION

This study on the effects of low molecular weight chitosan (LMC- 1) and shrimp preserving agentssuch as phytic acid (PA), sodium bisulfite (SB), and crustacean preservative (CP) on the preservation ofshrimp (Trachypenaeus curvirostris) and the bacteriostasis of LMC-1 showed that: (1) Different LMC-1conontration has different bacteriostasis on E. coIi, B. subtilis and S. aureou: (2) LMC-1 and CP arebetter than PA and SB for preserving the freshness of shrimp stored at 4℃.     

新疆巴里坤湖卤虫卵孵化率的研究

研究了我国新疆维吾尔自治区巴里坤湖（43.6°N,92.8°E）产的卤虫卵的采收方法、贮存条件对孵化率的影响和提高孵化率的几种化学和物理处理方法。据此设计了巴里坤湖卤虫卵的加工工艺流程,为进行规模性开发利用盐湖卤虫卵资源打下基础。     

海洋微藻的体外抗肿瘤活性初筛

采用小鼠乳腺癌细胞株tsFT210的细胞周期抑制、细胞凋亡诱导、细胞坏死以及对卤虫的毒性为活性指标,对48种海洋微藻的甲醇提取物的抗肿瘤活性进行了筛选。结果表明,以卤虫毒性为活性指标时,9种海洋微藻显示了不同程度的抗肿瘤活性,以tsFT210为筛选模型时,也有9种具有抗肿瘤活性,其中3种为明显的细胞凋亡诱导活性、2种为细胞坏死性活性、1种为G2/M期抑制活性,因此有必要进行开拓海洋微藻药用新资源的研究。     

The influence of shrimp farms organic waste management on chemical water quality

We evaluated the physical–chemical properties and nutrient concentrations in two shrimp ponds under conventional management, intensive (I-P) and semi-intensive (SI-P), and one under organic management (O-P), three days after loading and before stoking. The mean’s total alkalinity concentrations were 2.26 ± 0.04 mM, 2.28 ± 0.01 mM and 2.59 ± 0.01 mM, respectively for I-P, SI-P and O-P. Bicarbonate and carbonate accounted for 62% and 37% in I-P, 64% and 36% in SI-P and 83–17% in O-P, respectively. Aragonite and calcite were oversaturated around Ω = 5. Mean total phosphate (TP) concentrations were 441.37 ± 92.06 μg/L, 449 ± 48 μg/L and 473.64 ± 84.17 μg/L, under I-P SI-P and O-P management respectively. Following this sequence, NO₃⁻ concentration was 2.98 ± 0.7 μg/L, 1.16 ± 0.16 μg/L and 0.32 ± 0.12 μg/L, under I-P, SI-P and O-P management respectively. Thus, the data suggest that different management of farm organic waste leads to different chemical water quality.     

对虾鳗弧菌卵黄抗体(IgY)的制备及其对人工感染的保护研究

以纯培养的鳗弧菌作为抗原,探讨对虾鳗弧菌(Vibrio anguillarum)卵黄抗体(IgY)的制备技术,以及IgY在对虾体内的代谢及对人工感染和自然感染弧菌病的保护作用。结果表明:鳗弧菌对蛋鸡有较好的免疫原性,产蛋鸡经免疫后,血清与卵黄中很快出现抗体,而且卵黄中的抗体水平要高于血清中的抗体水平。IgY对鳗弧菌的感染有可靠的保护作用,IgY经口服后很快到达直肠,继而血淋巴、肝胰脏中也出现抗体,并可持续12~24 h;投喂IgY的试验组对虾在7 d内的死亡率为15%,极显著地低于对照组对虾的同期死亡率(为60%)。     

Optimal content and ratio of lysine to arginine in the diet of Pacific white shrimp, Litopenaeus vannamei

The optimal quantity of dietary lysine (Lys) and arginine (Arg), and the optimal ratio of dietary Lys to Arg for Pacific white shrimp Litopenaeus vannamei were investigated. Coated Lys and Arg were added to a basal diet (37.99% crude protein and 7.28% crude lipid) to provide graded levels of Lys and Arg. The experimental diets contained three Lys levels (2.51%, 2.11%, and 1.70% of total diet), and three Arg levels (1.41%, 1.80%, and 2.21% of total diet) and all combinations of these levels were tested. Pacific white shrimp, with a mean weight of 3.62±0.1 g, were randomly distributed in 36 fiberglass tanks with 30 shrimp per tank and reared on the experimental diets for 50 days. After the feeding trial, the growth performance, survival, feed conversion rate (FCR), body composition and protease and lipase activities in the hepatopancreases of the experimental shrimps were determined. The results show that weight gain (WG), specific growth rate (SGR), FCR, body protein, body Lys and Arg content were significantly affected by dietary Lys and Arg (P <0.05) and improved when dietary Lys and Arg levels were 2.11% ～ 2.51% and 1.80%～2.21%, respectively. Protease and lipase activities in the hepatopancreases of the shrimps appeared higher when dietary Lys and Arg quantities were 2.11% ～2.51% and 1.80%～2.21%, although the difference was not statistically significant (P >0.05). Therefore, according to our results, the optimal Lys and Arg quantities in the diet of Pacific white shrimp, L. vannamei, were considered to be 2.11%–2.51% and 1.80%–2.21%, respectively, and the optimal ratio to be 1:0.88–1:1.05.     

南海原油和燃料油对仔虾和仔鱼的急性毒性试验

测定了南海原油、0号柴油和20号柴油对斑节对虾Penaeusmonodon、日本对虾Penaeusjoponicus、刀额新对虾Metapenaeusensis3种仔虾和黄鳍鲷和Sparuslatus、黑鲷Sparusmacrocephalus、前鳞鲻MugilOphuyseni和七星鲈Lateolabraxjaponicus4种仔鱼的急性毒性。油类对仔虾和仔鱼的毒性大小顺序均为：0号柴油＞20号柴油＞南海原油。油类分散液的毒性大于其水溶性组分。在曝油的3种仔虾中,刀额新对虾对0号柴油和20号柴油的耐受力最弱,而日本对虾对南海原油最为敏感。曝油仔鱼对3种油类的耐受力顺序为：黄鳍绸＞黑鲷＞前鳞鲻,而七星鲈对0号柴油最为敏感。3种仔虾对油类的耐受力明显低于4种仔鱼。     

浙江沿岸海域虾类优势种空间生态位分析

生态位与种间竞争、资源利用密切联系,体现了物种在群落中利用资源的能力。为探明浙江沿岸海域虾类分布格局的形成和影响因素,通过相对重要性指数确定了虾类群落优势种,分别采用Levins公式和Pianka指数计测了虾类优势种生态位宽度和生态位重叠,并通过典范对应分析（CCA）研究生态位分化情况。结果表明:研究海域共出现13种虾类优势种,根据生态位宽度大小将研究海域内的虾类优势种分为3类,细巧仿对虾、细螯虾、鲜明鼓虾和中华管鞭虾属于第一类群,以上4种虾类的空间生态位宽度较宽;日本鼓虾、哈氏仿对虾、鹰爪虾、中国毛虾和葛氏长臂虾属于第二类群,这5种虾类空间生态位略低于第一类群,但其生态位仍相对较宽;戴氏赤虾、脊腹褐虾、东海红虾和大管鞭虾这4种虾类的空间生态位较窄,属第三类群;生态位重叠指数变化范围在0.00~0.78,将重叠指数按照大于0.6、0.3~0.6和小于0.3划分为重叠度较高、重叠度较低和重叠度低3个重叠度等级,结果表明,虾类优势种生态位重叠以较低和低2个等级为主;虾类分布受水深、底层溶解氧和底层盐度的影响较大,受水温影响较小。虾类群落优势种空间生态位与生活习性、种间竞争、摄食以及海洋环境因子等多种因素有关。     

基于Google Earth Engine的红树林年际变化监测研究

遥感技术已广泛应用于红树林资源调查与动态监测中,但仍然存在遥感数据获取困难、数据预处理工作量大、监测时间长而周期过大等问题,影响了学者对红树林演变过程的精细刻画与理解。本文基于Google Earth Engine（GEE）云遥感数据处理平台,选取Landsat系列卫星数据,生成长时间序列年际极少云影像集（云量少于5%）,利用3个红外波段反射率（NIR、SWIR1、SWIR2）和3个特征指数（NDVI、NDWI、NDMI）建立阈值规则集,实现对实验区越南玉显县红树林、红树林-虾塘、不透水面-裸地、水体4种目标地物的专家知识决策树分类和土地覆盖的制图,并基于分类结果监测该区域1993-2017年的红树林年际动态变化。结果表明：GEE平台可满足多云多雨地区红树林的长时间序列年际变化监测需求;本文阈值分类方法可以有效提取红树林及红树林-虾塘,实验区有86%年份的影像分类精度达到80%以上;年际变化监测可精细刻画实验区红树林面积先增后减再增的变化过程,也能准确反映红树林与红树林-虾塘养殖系统面积之间的负相关关系。红树林年际动态监测结果可以降低红树林演变分析的不确定性,并能更精细地量化红树林与其他土地覆盖类型的转化过程,从而评估经济发展、政策等因素对红树林演变的影响。