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31.
海洋微生物抗肿瘤活性菌株的分级组合筛选 总被引:10,自引:2,他引:8
为探索如何快速获取活性菌株的筛选模式 ,组合使用海虾生物致死法和tsFT2 10细胞的流式细胞术筛选模型 ,对从青岛前海和胶州湾潮间带海泥、动植物样品中分离的 162株海洋微生物 ,进行了抗肿瘤活性的分级组合筛选。经海虾生物致死法一级初筛 ,得到活性菌株 5 8株 (LC50 <5 0 0 μg/mL) ,阳性率为 3 5 .8%。对初筛有效的 5 8株菌利用流式细胞术筛选模型进行二级复筛 ,得到对tsFT2 10细胞有各种活性的活性菌株共 2 5株 ,阳性率为复筛菌株数的 43 .1%、初筛菌株总数的 15 .4% ,其中 ,诱发细胞坏死伴有凋亡诱导活性的菌株 7株 (占总菌株的 4.3 % ) ,具有细胞周期抑制活性的菌株 18株 (占总菌株的 11.1% )。该分级组合筛选模式与流式细胞术筛选模型的单独筛选模式相比 ,无漏筛 ,具有成本低、速度快、宜于大规模筛选等特点。 相似文献
32.
LU Xi LUAN Sheng CAO Baoxiang SUI Juan DAI Ping MENG Xianhong LUO Kun KONG Jie 《海洋学报(英文版)》2017,36(2):62-68
The aim of the present study is to detect the potential of the base population from diallel crosses of eight introduced strains of the Pacific white shrimp(Litopenaeus vannamei) for improving the yield. Heterosis and heritability were estimated for pond survival at commercial farm conditions for the base population that included 207 full-sib families from a nested mating design by artificial insemination. Among all the hybrids,the heterosis ranged from –11.37%(UA1×UA2) to 20.53%(UA3×SIN) with an average of 0.953%. The results showed that more than half of the hybrids(51.85%) have negative heterosis for survival rate, but most of the hybrids with positive heterosis have high estimates. The high proportion of negative heterosis for survival rate reminders us that the survival trait also should be considered in the crossbreeding program to avoid yield decrease. However, high positive heterosis manifested in most of the hybrids for survival indicates the usefulness of these hybrids for improving the survival to obtain higher yield by crossbreeding in this breeding program. The heritability estimate for pond survival was 0.092±0.043 when genetic groups were included in the pedigree, and it was significantly different from zero(P0.05). The results from this study also indicated that significant improvement for survival is possible through selection in L. vannamei. 相似文献
33.
The diverse clam shrimp Nestoria-Keratestheria fauna is widely distributed in the Dabeigou Formation in northern Hebei and eastern Inner Mongolia of China.Its important component genus Magumbonia from the Dabeigou Formation in the Luanping Basin,northern Hebei,China,is revised on the basis of a scanning electron microscope (SEM) examination of the type species M.jingshangensis Wang,1984,which revealed morphological features not recognized previously.These include coarse reticulation on the umbo and promi... 相似文献
34.
Matt K. Broadhurst Russell B. Millar Damian J. Young Michael E. L. Wooden Stuart Rowland 《新西兰海洋与淡水研究杂志》2013,47(5):755-766
Three manipulative experiments were done to estimate the selectivity of conventional and new sizes and configurations of mesh for school prawns, Metapenaeus macleayi, in three south‐eastern Australian recreational fishing gears (haul, push, and scoop nets). The treatment meshes examined were: (1) conventional‐sized, diamond‐shaped mesh used in all gears (20 mm in scoop nets and 30 mm in push and haul nets); (2) 30 mm in scoop nets; and (3) 40‐mm diamond‐ and (4) 23‐mm square‐shaped mesh in all gears. In all experiments, known quantities of school prawns (6–24 mm carapace length (CL)) were placed in purpose‐built enclosures, monitored to ensure no experimental‐induced stress (as measured by changes in L‐lactate in their haemolymph) and the replicate treatments of the various gear configurations deployed. Escapees from the various treatment nets were collected from the enclosures using fine‐meshed nets. Logistic selection curves were derived for all treatment nets and specific comparisons made within and among gears. All nets had 50% retention lengths (L50) comparable to other penaeid‐catching gears with similar mesh sizes, but most had selection ranges (SRs) that were atypically inflated. The large SRs were attributed to a combination of factors that included the mesh geometry and towing speed of the gears and the behaviour of school prawns. The 20‐mm scoop net had the smallest selection parameters, retaining >99% of individuals larger than 13 mm CL. Mesh size in this gear would need to be increased to at least 30 mm to allow some maturing prawns (>18 mm CL) to escape. 相似文献
35.
Rita Cannas Flavio Sacco Maria Cristina Follesa Andrea Sabatini Marco Arculeo Sabrina Lo Brutto Teresa Maggio Anna Maria Deiana Angelo Cau 《Marine Ecology》2012,33(3):350-363
Genetic variation at eight microsatellite loci was studied in nine populations of the blue and red shrimp Aristeus antennatus to investigate whether distinct stocks are present in the Western Mediterranean Sea. A high level of gene flow and no evidence of genetic partitioning were discovered. No significant variation was found (FST = 0.00673, P-value = 0.067) even when shrimps from exploited and those from deep-water unexploited grounds were compared. No evidence of reduction or expansion of population size in the recent past was found, as indicated by the bottleneck and interlocus g-tests. Our results are consistent with previous studies using mitochondrial gene methods and allozymes, indicating that, for this species, extensive pelagic larval dispersal and adult migration are probably responsible for the genetic homogeneity observed. In particular, due to a different bathymetric distribution of males and females, reported to be associated with different water masses and hence with possible differential dispersal capacity between sexes, the hypothesis of sex-biased dispersal was tested. Mean values of corrected assignment indices and mean relatedness values were higher for males, suggesting that females are the more widely dispersing sex. Molecular assessment of A. antennatus from the Western Mediterranean provides data of biological and evolutionary interest for the successful management of such a highly valuable fishery resource. 相似文献
36.
为探讨虾血对抑制兔耳瘢痕增生的影响,以成年新西兰大耳白兔建立兔耳增生性瘢痕模型,将增生性瘢痕块分为对照组(A组)、虾血治疗组1(B组)和虾血治疗组2(C组),以不同组瘢痕组织石蜡包埋HE染色,观察虾血治疗对瘢痕组织中成纤维细胞、胶原纤维的影响。结果表明:肉眼观察虾血治疗组的瘢痕较空白对照组有改善;光镜下见虾血治疗组成纤维细胞减少,胶原纤维含量降低且排列较规则有序,差异具有显著性(p<0.001);虾血治疗组2微血管均数较少,抑制疤痕增生效应最好(p<0.01);虾血可能具有抑制兔耳增生瘢痕的形成及瘢痕组织增生的作用。 相似文献
37.
The fossil dendrobranchiate shrimp Macropenaeus was originally described from the Upper Cretaceous (Cenomanian) limestones of Hadjoula, northwest Lebanon. A new species, M. sidiaichensis sp. nov., was recovered from the Sidi Aïch Formation in the Northern Chotts Range, southern Tunisia. The Barremian occurrence of the genus in Tunisia suggests that Macropenaeus most likely originated in northern Africa and then migrated to Arabia in the Cenomanian. The association of the fossil shrimp with conchostracans, plant fragments, and fish indicates the interaction of freshwater and marine conditions that characterized the northern African margin as part of widespread coastal complex, paralic environments during the Barremian. 相似文献
38.
虾类微量元素含量的主成分分析 总被引:2,自引:0,他引:2
应用主成分分析法对湛江几种养殖对虾和海捕天然对虾的微量元素的含量进行综合评价。结果表明:虾体中微量元素含量均衡;综合排名依次为野生斑节对虾、野生凡纳滨对虾、养殖斑节对虾、养殖凡纳滨对虾,两种野生对虾的综合排名均高于养殖对虾;野生斑节对虾综合得分为1.6708,微量元素含量最均衡,营养价值最高,养殖凡纳滨对虾营养价值最低,综合得分为-1.20943。 相似文献
39.
��� 《中国海洋湖沼学报》2011,29(1):136-140
We collected 3 596 Metapenaeopsis stridulans (Decapoda: Penaeidae) from the southeast coast of India between January and December 2007. Sixty three specimens (43 females and 18 males) were parasitized by the bopyrid isopod Epipenaeon ingens (Isopoda: Bopyridae). This is the first report of the occurrence of E. ingens in this host; therefore, it was considered as a new host record. The highest level of infestation (3.2%) occurred in October 2007, coincident with observations of gravid females (9). The total prevalence and presence of gravid females were 17.46% and 28%, respectively. Infestation caused a characteristic bulge of the branchial chamber, growth retardation, and degeneration of the sex organs, but had no effect on the host weight. 相似文献
40.
对凡纳滨对虾的养殖采用新的投料和管理方法,不施肥,从放苗后2h开始投料,并由2餐/d改为5餐/d;管理上采用不施抗生素药物的生态养殖模式。定期抽样测量对虾的体长。结果表明,实验对虾生长速度比对照塘提高20%~30%,成活率高达86.33%,比对照塘高约20%。实验证明该方法值得推广。 相似文献