一例异育银鲫(Carassius auratus gibelio)暴发性出血病病原分析

2016年6月,江苏某异育银鲫(Carassius auratus gibelio)养殖场暴发一种传染性急性出血病,造成养殖银鲫大量死亡。为分析此次疾病病因及流行规律,本研究从发病养殖场采集患出血病的异育银鲫,从细菌、病毒及寄生虫三个方面对病原进行了分析。采用病原菌分离、组织病理学观察、超薄切片电镜观察、病毒核酸分析、回感实验等对病原进行鉴定。结果显示从发病鲫鱼体内分离到病毒一株,未发现寄生虫及细菌感染。经测序及序列分析,该病毒为鲤疱疹病毒Ⅱ型(Cyprinid herpesvirus2,CyHV-2)病毒,组织病理学观察结果显示患病鱼的鳃和肾脏有明显病变,电镜下可观察到病鱼脾脏组织有带囊膜的球形病毒,囊膜直径约为170—200nm,病毒衣壳直径约为110—120nm,核心直径约为60nm,用组织匀浆感染鲫鱼囊胚细胞系(CGB)可稳定地观察到典型的细胞病变,用患病鱼组织匀浆液人工感染异育银鲫的死亡率高达100%,荧光定量PCR检测到该病毒可感染多器官,其中以脾脏中病毒含量最高,其次是脑,肝脏中最少。本研究可为CyHV-2的诊断防控及疫苗研制提供资料。     

岬湾海岸中人工岛建设对岬角涡旋及海湾地形冲淤的影响——以海南岛铺前湾为例

岬湾海岸中一般在岬角的右侧与左侧（面向大海方向）分别发育顺时针与逆时针涡流。这些涡流的存在对泥沙输运、海底地形演变、污染物扩散等都具有重要影响。而在海洋中岛屿的存在将产生局部的岛后尾流。如岛屿与岬角距离较近,岛后尾流可能与岬角涡旋相互作用,反之则相互影响较小。本文以海南岛铺前湾为例,研究在岬湾海岸中湾口处的人工岛建设对湾内涡旋的影响,并进一步分析人工岛建设对整个海湾海底冲淤变化的效应。本项研究主要采用COAWST模型进行研究。结果表明,大潮期在海湾内发育大范围的顺时针涡旋,小潮期涡旋发育不明显,以东向余流为主。人工岛建设并未影响整个海湾的涡旋结构,但人工岛分隔了湾内顺时针涡旋,且在周围产生局部的逆时针与顺时针涡旋。海底冲淤变化的基本格局为湾内以淤积为主,人工岛建设加强了这种趋势,但在人工岛与岸线之间的狭窄通道内,海底出现冲刷。本项研究对岬湾海岸的科学开发管理具有一定意义。     

天然气水合物的声学探测模拟实验

在人工岩心中进行天然气水合物的生成和分解实验,同时获取了体系的温度、压力、声学特性(Vp和Vs、幅度和频率)及含水量等参数。经研究发现,温压法、超声探测法和TDR探测法都能灵敏探测沉积物中天然气水合物的形成和分解过程。分析认为,本次实验中水合物形成速率过快,只能宏观研究水合物对沉积物声学特性的影响,建议采用长岩心进一步研究沉积物中水合物的声学特性。     

Field Study on the Behavior of Destructive and Non-Destructive Piles Under Compression

This paper presents a series of full-scale load tests on long bored piles instrumented with strain gauges along the shafts, including eight field tests of piles loaded to failure and one non-destructive pile load test. The load-displacement response, skin friction, end resistance, and the threshold of the pile-soil relative displacement for fully mobilizing skin resistance were discussed. A simple softening model was proposed to describe the degradation behavior of the skin friction along the pile-soil interface and the load-displacement relationship developed at the pile base. It is found that the shaft resistance degradation investigated in the non-destructive load test only occurs at a shallow depth, and the skin friction of deeper soil is not fully developed. However, unlike the results of the non-destructive load tests, the softening is accompanied by a reduction in skin friction and observed to be along the whole pile depth. The thresholds of pile-soil relative displacement for fully mobilizing skin resistances in different soils have been found to be in the range 0.6% to 2.4% of the pile diameter. Moreover, in practical applications, a bilinear model is assumed to be feasible in analyzing the load-settlement relationship developed at the end of non-destructive pile, whereas the load transmission curve of the soils below the pile base corresponds to a softening model in the field tests of piles loaded to failure.     

Field and Theoretical Analysis on the Response of Destructive Pile Subjected to Tension Load

Most field tests are carried out using working piles for verification purposes in China, and loading tests are terminated before achieving true pile capacity. In this work, two full-scale destructive loading tests on tension piles instrumented with strain gauges were conducted to capture true pile capacity. The load-displacement response, load transfer, and threshold of the pile-soil relative displacement for fully mobilizing skin resistance in the uplift case were discussed. It was found that the shaft resistance degradation is observed to be along the pile depth with a reduction factor of 0.905 to 0.931, and the thresholds of pile-soil relative displacement for fully mobilizing skin resistance of the tension pile in different soils are found to be in the range 0.67% to 1.34% of the pile diameter. Based on the field test results, a simple softening model was proposed to describe the degradation behavior of skin friction along the pile-soil interface. Further study was conducted to assess the influence of the threshold of pile-soil relative displacement for fully mobilizing skin friction and the reduction factor on the skin friction. As to the analysis of the response of single pile subjected to tension load, a highly effective iterative computer program was developed using the proposed skin friction softening model. Comparisons of the load-settlement response for the well-instrumented tests were given to demonstrate the effectiveness and accuracy of the proposed simple method.     

The invertebrate fauna of a forest stream and its association with fine particulate matter

The invertebrate fauna colonising wooden blocks placed in a beech forest stream was studied over a period of 1 year. At least 31 species were taken during the course of the study, with 3 species of Chironomidae predominating numerically. Most species were associated principally with fine particulate matter (FPM) deposited on the upper surfaces of blocks, although a stonefly, Spaniocerca zelandica, was most abundant on the barer surfaces. Of the Chironomidae, Paucispinig‐era approximata and Polypedilum canum had clearly defined life history patterns with emergence from February to April and October to January, respectively. Psectrocladius sp. had a poorly defined life history. Gut contents of 16 insect species showed that 4 were predominantly predatory, and the remainder were detritivores. The latter could be categorised as shredders (large particle detritivores) or collector‐browsers which mainly ingested particles in the dominant (<50 μm) size range. Oxygen consumption by fine stream sediments was similar at different times of year which suggests that the food quality of detritus may tie relatively constant at all times.     

曹妃甸浅海人工岛工程海底泥沙冲淤

浅海人工岛建设改变了周边海洋环境动力边界条件,引起周边海域泥沙冲淤变化。对卫星遥感数据和地形测量数据进行了对比分析,结合研究区风浪、潮流、悬浮泥沙分析了海底地形变化原因,研究了周边海域的泥沙冲淤规律,并对淤积和侵蚀的程度进行了量化评价,提出可以对护岛潜堤进行优化设计,此研究对类似浅海人工岛建设具有指导作用。     

基于神经网络的南太平洋长鳍金枪鱼渔场预报

南太平洋长鳍金枪鱼是我国远洋渔业的重点捕捞对象;对南太平洋长鳍金枪鱼进行准确的渔场预报;可以提高捕捞效率;提高渔业的生产能力。本研究根据1993-2010年南太平洋长鳍金枪鱼的延绳钓生产数据以及海洋卫星遥感数据（海水表面温度;SST;海面高度;SSH）和ENSO（El Niño-Southern Oscillation）指标;采用DPS（data processing system）数据处理系统中的BP人工神经网络模型;以渔获产量（单位时间的渔获尾数）和单位捕捞努力量渔获量（CPUE;Catch per unit of effort）分别作为中心渔场的表征因子;并作为BP模型的输出因子;以月、经度、纬度、SST、SSH和ENSO指标等作为输入因子;分别构建4-3-1;5-4-1;5-3-1;6-5-1;6-4-1;6-3-1等BP模型结构;比较渔场预报模型优劣。研究结果表明;以CPUE作为输出因子的BP人工神经网络结构总体上较优;其中以6-4-1模型结构为最优;相对误差只有0.006 41。研究认为;以CPUE为输出因子的6-4-1结构的人工神经网络模型;能够准确预报南太平洋长鳍金枪鱼的渔场位置。     

缢蛏(Sinonovacula constricta)规模增殖放流对三沙湾滩涂底质环境改良的效果

于2013-2014 年在三沙湾选择增殖放流区Ⅰ区和Ⅱ区, 开展了2 批次的缢蛏增殖放流, 计 放流缢蛏苗数量约3747 万粒。采用对缢蛏生长、生物量动态变化和滩涂底质主要因子跟踪监测的方 法, 开展对缢蛏增殖放流效果的评估。研究结果, 2 个增殖放流区的缢蛏密度和缢蛏生物量都比对照 区有极显著提高, 共收获65 吨缢蛏大规格苗, 增殖放流产出比为1: 8.0-9.0, 获得了一定的资源增 殖效果和经济效益。放流期间, 滩涂底泥中的氧化还原电位、硫化物和有机碳等浓度在增殖区Ⅰ区、 Ⅱ区和对照区之间都无显著差异(P>0.05), 但, 底泥中Ⅰ区、Ⅱ区的氧化还原电位平均值分别比对照 区提高了26%和18%, 硫化物浓度平均值分别比对照区降低了45%和34%, 有机碳浓度平均值分别 比对照区下降了5.8%和6.8%, 表明, 缢蛏增殖放流获得了一定的改良底质环境效果。     

Changes of soil surface roughness under water erosion process

The objective of this study was to determine the changing characteristics of soil surface roughness under different rainfall intensities and examine the interaction between soil surface roughness and different water erosion processes. Four artificial management practices (raking cropland, artificial hoeing, artificial digging, and contour tillage) were used according to the local agriculture customs of the Loess Plateau of China to simulate different types of soil surface roughness, using an additional smooth slope for comparison purposes. A total of 20 rainfall simulation experiments were conducted in five 1 m by 2 m boxes under two rainfall intensities (0.68 and 1.50 mm min^?1) on a 15° slope. During splash erosion, soil surface roughness decreased in all treatments except raking cropland and smooth baseline under rainfall intensity of 0.68 mm min^?1, while increasing for all treatments except smooth baseline under rainfall intensity of 1.50 mm min^?1. During sheet erosion, soil surface roughness decreased for all treatments except hoeing cropland under rainfall intensity of 0.68 mm min^?1. However, soil surface roughness increased for the artificial hoeing and raking cropland under rainfall intensity of 1.50 mm min^?1. Soil surface roughness has a control effect on sheet erosion for different treatments under two rainfall intensities. For rill erosion, soil surface roughness increased for raking cropland and artificial hoeing treatments, and soil surface roughness decreased for artificial digging and the contour tillage treatments under two rainfall intensities. Under rainfall intensity of 0.68 mm min^?1, the critical soil surface roughness was 0.706 cm for the resistance control of runoff and sediment yield. Under rainfall intensity of 1.50 mm min^?1, the critical soil surface roughness was 1.633 cm for the resistance control of runoff, while the critical soil surface roughness was 0.706 cm for the resistance control of sediment yield. These findings have important implications for clarifying the erosive nature of soil surface roughness and harnessing sloped farmland. Copyright © 2013 John Wiley & Sons, Ltd.