蟹类微卫星DNA标记的筛选及其在遗传学研究中的应用

微卫星DNA标记是近年来最受研究者青睐的分子标记之一.由于其具有高度多态性、共显性遗传、基因组中含量丰富且随机分布等特点,已被应用于种群分化研究、亲缘分析、基因连锁分析、进化以及生态学研究等许多领域.近年来,蟹类微卫星的研究报道日益增多.文中对蟹类微卫星分离方法、引物设计、遗传学特性以及在种群遗传、家系分析、遗传多样性评价等方面的最新研究进展进行综述,并分析微卫星分析中无效等位基因(null allele)、"结巴"带(stutter bands)和上游等位基因"扩增丢失"现象(upper allelic dropout)的产生原因以及对微卫星基因型判读带来的影响.     

Implementing geostatistical analysis to study spatio-temporal distribution patterns of swimming crabs(Portunus trituberculatus)

This study attempted to compare the performance of local polynomial interpolation,inverse distance weighted interpolation,and ordinary kriging in studying distribution patterns of swimming crabs.Cross-validation was used to select the optimum method to get distribution results,and kriging was used for making spatial variability analysis.Data were collected from 87 sampling stations in November of 2015(autumn) and February(winter),May(spring) and August(summer) of 2016.Results indicate that swimming crabs widely distributed in autumn and summer:in the summer,they were more spatially independent,and resources in each sampling station varied a lot;in the winter and spring,the abundance of crabs was much lower,but the individual crab size was bigger,and they showed the patchy and more concentrative distribution pattern,which means they were more spatially dependent.Distribution patterns were in accordance with ecological migration features of swimming crabs,which were affected by the changing marine environment.This study could infer that it is applicable to study crab fishery or even other crustacean species using geostatistical analysis.It not only helps practitioners have a better understanding of how swimming crabs migrate from season to season,but also assists researchers in carrying out a more comprehensive assessment of the fishery.Therefore,it may facilitate advancing the implementation in the pilot quota management program of swimming crabs in northern Zhejiang fishing grounds.     

Snowmelt detection from QuikSCAT and ASCAT satellite radar scatterometer data across the Alaskan North Slope

The timing of seasonal snowmelt in high-latitude tundra has implications ranging from local biological productivity to global atmospheric circulation, yet remains difficult to quantify, particularly at large spatial scales. Snowmelt detection in such remote polar environments is possible using satellite-based microwave scatterometers, such as NASA’s QuikSCAT. QuikSCAT measured scattering in Ku-band, which is sensitive to snowmelt signals, from 1999 until the antenna failed in 2009. The Advanced Scatterometer (ASCAT) (2006–2021 (projected) operational), which operates at C-band, may be able to extend the QuikSCAT record, but existing techniques fail to adequately monitor tundra environments. Here, we designed a departure threshold algorithm to produce a consistent 15-year time series of melt onset for the tundra of the Alaskan North Slope, using the overlap period for the enhanced resolution datasets to calibrate the ASCAT melt detection record against QuikSCAT. We produced a time series of day of year of melt onset for 4.45 km x 4.45 km grid cells on the Alaskan North Slope from 2000–2014. Time series validation with in situ mean daily air temperature produced mean R² values of 0.75 (QuikSCAT) and 0.72 (ASCAT). We qualitatively observed a difference between early-season melt, which occurred rapidly and was driven by strong wind events, and more typical melt, which occurred gradually along a latitudinal gradient. We speculate that future melt timing will have greater frequency of early-season onset as climate change destabilizes the high-latitude atmosphere.     

Distribution and retention of Petrolisthes armatus in a coastal plain estuary: The role of vertical movement in larval transport

Since transport of planktonic larvae is essential to the maintenance and expansion of many marine species, we examined the spatial and temporal distribution of green porcelain crab Petrolisthes armatus (Gibbes, 1850) larvae and the possible underlying physical and behavioral mechanisms using a combination of field observations and numerical modeling. The field study consisted of observations of larval abundance and distribution as well as hydrographic surveys of the Satilla River estuary on the east coast of the USA in August 2006. Larvae were found throughout the water column within the tributaries but primarily at depth in the main river. A numerical model was used to examine the effect of flow and possible larval behavior responsible for the observed distribution and the consequences for larval retention in the estuary. Model results that included downward larval movement are consistent with the field observations, supporting the hypothesis that P. armatus larvae vertically migrate within the water column, which aids in their retention within the estuary.     

Reproductive periodicity of the tropical crab Callinectes arcuatus Ordway in Central America

The Gulf of Nicoya, an estuary on the Pacific coast of Central America, contains a large population of the portunid crab Callinectes arcuatus. Results of a 12 month survey indicated that spawning activity occurs throughout the year, but with a distinct peak during the five-month dry season (December-April). Mature females were most prevalent in the upper regions of the gulf during the rainy season and appeared to migrate to the lower gulf to spawn during the dry season. Patterns of spawning and apparent migration differed from those reported in an earlier study of C. arcuatus along the Pacific coast of Mexico.     

Population biology of the portunid crab Callinectes arcuatus Ordway in the Gulf of Nicoya,Costa Rica,Central America

Tropical blue crabs Callinectes arcuatus were collected by trawling in the Gulf of Nicoya on the Pacific coast of Costa Rica, Central America. The gulf population was generally dominated by females. Adult females were common in the upper, more estuarine regions of the gulf during rainy season, but appeared to migrate to the lower gulf during dry season for spawning. Biomass varied with seasonal changes in abundance, but was generally similar to biomass of C. sapidus in Chesapeake Bay. Analysis of size frequency indicated that the population is numerically dominated by adults during January and February and that juveniles are common during the remainder of the year. Extrapolation of available data suggests that female crabs reach maturity in approximately one year after hatching.     

Field experiments on the role of epibenthic predators in determining prey densities in an estuarine mudflat

A series of caging experiments was performed on an estuarine mudflat at three seasons of the year, in which Carcinus maenas L. and Pomatoschistus microps (Kroyer) were either excluded from, or allowed to prey upon, the benthos in order to determine to what extent infaunal abundance and mortality was a result of predation by epibenthic predators.The difficulties of conducting and interpreting the results of such experiments are recognized. The benthic macrofauna of this mudflat is dominated numerically by small annelids and there is evidence that adult C. maenas can cause significant increases in the oligochaete component of this assemblage, probably as a result of disturbance caused by its burrowing activity. Juvenile C. maenas on the other hand significantly reduced the abundance of small annelids, particularly the dominant polychaete Manayunkia aestuarina (Bourne) and could be responsible for year-to-year variations in abundance of this species. The role of fish predators (in this case P. microps) is more problematical but it is suggested that in the densities at which they occur naturally on the mudflat they have little direct effect on the abundance of prey species. There is no evidence that seasonal mortality of small annelids is reduced in the absence of predation and this is taken to indicate that not all mortality is due to epibenthic predation. Certain changes in relative abundance of the component species of the harpacticoid copepod community were discerned but it is suggested that the plasticity of their reproductive potential is such that the effect of predation on the group as a whole is usually masked.     

祁连山地区镁铁——超镁铁岩的多样性

祁连山地区出露有不同类型的镁铁－超镁铁岩，包括蛇绿岩，阿拉斯加型岩体，橄榄岩－闪长岩型岩体以有产于北祁连西部的一种特殊的镁铁－超镁铁岩组合。不同的镁铁－超镁铁岩形成于一同的构造环境，蛇绿岩代表洋壳的残片，阿拉斯加型岩体产生岛弧或活动陆缘环境，橄榄岩一闪长岩体则是板块碰撞之后形成的，根据蛇绿岩与大致同时代的阿拉斯加型岩体产出的位置，推测在北祁连早古生代时存在向南的消减事件。