AMS测量海水129I的气载分离制样

本研究建立了AMS(Accelerator mass spectrum,加速器质谱)测定海水中129 I的气载分离制样方法:对加入载体的海水样品进行氧化还原处理,在加热的条件下将生成的单质碘吹出,并使用吸收装置吸收,后经阴离子交换树脂富集纯化,最后生成用于AMS分析的AgI沉淀,本方法全程回收率50%~70%,在相对密闭的体系中进行,减少了碘的损失,相对于萃取-反萃取的制样方法具有可操作性强,避免有机试剂使用等优点,可用于固态、液态和气态样品中129 I的AMS分析制样。     

饥饿对中国龙虾叶状幼体Ⅰ期发育、摄食及存活的影响

中国龙虾叶状幼体Ⅰ期在饥饿条件下的生长发育、变态、存活的研究结果表明:初孵叶状幼体耐饥饿能力较强,完全饥饿144h,成活率为100%,饥饿192～216h,成活率为76%～68%.由生长和变态来看,推迟24～36h投饵,对叶状幼体Ⅰ期的生长没有明显影响.在摄食饵料方面,初孵幼体饥饿144h,仍然有55%的幼体具有摄食能力.饥饿24～120h的初期叶状幼体摄食卤虫幼体的大小(体长为349.5～604.9μm)可达到自身体长的1/2～1/3.当叶状幼体连续摄饵时间达60h,体内储存一定的营养后,即使不再喂食,幼体也能变态,但变态率较低.由间隔投饵效果来看,每隔8,16,24h投饵,幼体的发育变态差异不大.这些研究结果对开展龙虾基础生物学与人工育苗技术研究,提高育苗成活率将有一定的参考意义.     

Sympatric seagrass shrimp show similar structure and selection along the seashore: a comparison of two cryptic Phycomenes zostericola lineages

Small crustaceans are diverse and abundant in seagrass habitats and provide many ecological services. Further information is needed about their long‐ and short‐term response to habitat fragmentation and loss. Shrimp specimens were sampled from coastal seagrasses: latitudes 9°–27° S (Torres Strait to Moreton Bay, Queensland, Australia) and longitudes 141°–153° E (Weipa to Moreton Bay, Queensland, Australia). The seagrass shrimp Phycomenes zostericola (Caridea: Decapoda) underwent phylogeographic analysis using mitochondrial and nuclear sequence data. Lineage relationships were investigated and two deeply divergent sympatric lineages of P. zostericola were discovered in northern samples. The two lineages display vastly different demographic histories in spite of similar overall phylogeographic patterns. The two lineages appear to be in secondary contact with each other, detailing independent histories of sea level change, long distance colonisation and habitat fragmentation. Their regional structure is evidence that the potential for dispersal is only realised under specific environmental conditions. Nuclear data, used to determine reproductive status between lineages, instead displayed a non‐random latitudinal geographic distribution suggesting directional selection, possibly in response to ocean temperatures. The differences between the two deeply divergent lineages highlight the importance of understanding variability among cryptic taxa and their response to habitat change.     

三疣梭子蟹(Portunus trituberculatus)I 型Crustin抗菌肽的基因克隆与真核重组表达

Crustin 是目前报道广泛存在于甲壳类中的一类具有广谱抗菌活性的抗菌肽, 主要由甲壳类血淋巴细胞合成。本研究采用RACE 技术和真核表达技术, 进行了三疣梭子蟹Crustin 基因的克隆和重组表达研究。结果表明, 在三疣梭子蟹血细胞Crustin 基因cDNA 全序列中, 开放阅读框编码110个氨基酸残基的Crustin 前体, Crustin 前体由N 端21 个氨基酸残基的信号肽、富含Cys 结构域和C端WAP 结构域三部分组成, 属于I 型Crustin, 构建的分泌型真核表达质粒pVT102U/α-crustin 转化酿酒酵母S78, 经RT-PCR 和SDS-PAGE 电泳验证, 表明重组Crustin 表达成功。     

页岩I型断裂韧性的分形计算方法与应用

水平井分段体积压裂是页岩气商业规模开发的重要工艺措施,如何评价页岩储层可压性是该工艺成功的关键。页岩断裂韧性是可压性评价的重要支撑参数,从I型断裂裂纹（裂缝）微观形态入手,结合断裂力学理论和分形理论,建立了页岩I型断裂韧性分形计算方法;借助晶体劈裂功法计算页岩表面能,采用密度、声波时差2种参数获取计算结果,对比分析新的分形方法计算数据、传统方法预测数据与试验测试数据,新的分形方法计算平均误差为3.63%,传统预测方法平均误差为 %,验证了方法的准确性;参考实例水平井测井解释数据,计算了水平段I型断裂韧性指数的全井筒连续性剖面,结合可压性级别与较大储层改造体积概率关系,优选可压性级别为III级及II级改造。建立的页岩I型断裂韧性分形计算方法对定量评价页岩储层可压性具有重要意义。     

青藏工程走廊冻土环境工程地质区划及评价

文章采取层次分析和综合评判的三级区划方法,分别考虑冻土的类型、热稳定性和含冰（水）量,将西大滩至安多间的青藏工程走廊划分为3个工程地质区、20个亚区和51个地段,按区（段）简要评价了冻土工程地质条件和寒区环境.文章对青藏工程走廊进行了较全面的冻土工程和寒区环境工程地质综合评价,能为工程设计、施工和运行维护、融冻灾害整治和环境管理提供科学依据.     

黔桂地区石炭纪I型层序界面沉积记录及成因分析

黔桂地区石炭纪 10个层序中的S1、S3、S6、S9、S10 5个Ⅰ型层序底界面和S10 层序顶界面具有特征的沉积记录 ,表现为岩性岩相的突变 ,侵蚀平行不整合和上超不整合发育 ,风化残积硅铝、铁铝和铝土质粘土层、残积角砾岩、淡水成岩相及白云岩化广泛发育 ,生物化石组合的巨大变化和C、O、Sr同位素值的明显差异。这些Ⅰ型界面是由不同的成因事件形成的。研究结果表明 ,S1底界面对应的是构造事件 ,即柳江运动 ;S10 顶界面反映的构造事件是黔桂运动 ;S3、S6、S9、S10 底界面均是由海平面下降事件形成     

Late Quaternary climate-driven environmental change in the Larsemann Hills, East Antarctica, multi-proxy evidence from a lake sediment core

Little is known about the response of terrestrial East Antarctica to climate changes during the last glacial-interglacial cycle. Here we present a continuous sediment record from a lake in the Larsemann Hills, situated on a peninsula believed to have been ice-free for at least 40,000 yr. A mutli-proxy data set including geochronology, diatoms, pigments and carbonate stable isotopes indicates warmer and wetter conditions than present in the early part of the record. We interpret this as Marine Isotope Stage 5e after application of a chronological age-depth model and similar ice core evidence. Dry and cold conditions are inferred during the last glacial, with lake-level minima, floristic changes towards a shallow water algal community, and a greater biological receipt of ultraviolet radiation. During the Last Glacial Maximum and Termination I the lake was perennially ice-covered, with minimal snowmelt in the catchment. After ca. 10,500 cal yr B.P., the lake became seasonally moated or ice-free during summer. Despite a low accumulation rate, the sediments document some Holocene environmental changes including neoglacial cooling after ca. 2450 cal yr B.P., and a gradual increase in aridity and salinity to the present.     

Polycrystalline amphibole aggregates (clots) in granites as potential I‐type restite: An ion microprobe study of rare‐earth distributions

Aggregates of polycrystalline grains of amphibole (clots) occur widely in the granodiorites of the Strontian pluton, Scotland. These clots are complex structures with numerous small grains in the interior exhibiting zonation from actinolite cores to hornblende rims. Amphiboles in the outer parts of these clots are indistinguishable from hornblendes that have crystallised from the melt. A rare‐earth element (REE) study of individual amphibole and pyroxene grains using an ion microprobe has also shown a marked difference in REE abundances, with clots generally being depleted in their interiors. Modelling of the compositions shows that the clots are consistent with being derived from pyroxene + plagioclase ± amphibole precursors. These granular precursors are recognised as being consistent with the residual crystalline material encountered in dehydration melting experiments of amphibolitic starting materials. It is suggested that these features could represent restite in I‐type granodiorites and tonalites. Extensive, but incomplete equilibration of the clot material provides an explanation for the infrequent identification of restite (other than grain cores) in I‐type granites, in marked contrast with S‐types.     

Application of I / I to define the source of hydrocarbons of the Pol-Chuc, Abkatún and Taratunich–Batab oil reservoirs, Bay of Campeche, southern Mexico

Homogeneous ¹²⁹I / ¹²⁷I ratios from 6.51 ± 1.36 × 10^− 14 to 12.6 ± 1.49 × 10^− 14 were measured in formation brine at the Pol-Chuc, Abkatún, Taratunich–Batab off-shore oil reservoirs, Bay of Campeche in S-Mexico. Cosmogenic production could account for a homogeneous, Late Cretaceous/Paleocene time period (71.3 ± 5.3 to 56.3 ± 2.9 Ma) for the sedimentation and burial of organic material in the source formation. As the actual reservoir column is formed by Paleocene to Kimmeridgian sediments, the lower part of the lithological column must have received hydrocarbons that migrated downward from an initial source rock (Upper Cretaceous?) during a post-Paleocene event, probably during Miocene. Cosmogenic production from Tithonian shales can be excluded, as ¹²⁹I would have been decayed. As an alternative or complementary process, the subsurface, radiogenic production of ¹²⁹I / ¹²⁷I by ²³⁸U-fission in Uranium-enriched sediments should also be considered to explain the present, low ¹²⁹I / ¹²⁷I ratios.