The Silurian Rugose Corals of Northern and Eastern Yunnan

正 With 1 Plate. The material dealt with in this paper was collected by the late Mr. W. Y.Lin' from Takuan, Northern Yunnan~2 and by Professor P. Misch from near the Lufengtsun Station~(?) of the Yunnan-Indo-China Railway in 1939; and also by various xcursions of the Department of Geology of the National University of Peking to     

两相法合成Methomyl的研究

报道一种合成Methomyl的两相法。此法能使甲硫基乙醛肟与甲胺基甲酰氯之间的缩合反应在两相界面上进行,从而有效抑制酰氯的水解,并使收率明显提高。     

氟甲砜霉素在水产养殖中应用的研究进展

氯霉素是一种有效的广谱抗生素。但是,氯霉素存在严重的毒副作用,能抑制人体骨髓造血功能,引起人类的再生障碍性贫血、粒状白细胞缺乏症、新生儿和早产儿灰色综合症等疾病,低浓度的药物残留还会诱发致病菌的耐药性,因此水产品中的氯霉素残留对人类的健康构成了巨大威胁[1,2]。     

米曲霉两步活化法对黄芩苷的生物转化及转化产物的纯化与鉴定

利用米曲霉把黄芩苷转化为黄芩素。转化条件为:液体发酵,pH6,30℃,利用综合马铃薯培养基与玉米芯培养基两步发酵。菌种采用两步活化法,将经综合马铃薯培养基培养48 h的米曲霉加入至玉米芯培养基培养48 h后,再加入底物黄芩苷继续培养3 d。玉米芯发酵液加甲醇混匀后过滤,通过高效液相色谱检测,结果表明米曲霉已经将黄芩苷转化为黄芩素,转化率为40.72%。同时用乙醚萃取法从玉米芯发酵液中萃取到黄色固体提取物,经过高效液相色谱与标准品对照鉴定,该提取物为黄芩素。     

软甲纲(节肢动物门:甲壳动物亚门)系统发育线粒体基因组研究

Along with the sequencing technology development and continual enthusiasm of researchers on the mitochondrial genomes, the number of metazoan mitochondrial genomes reported has a tremendous growth in the past decades. Phylomitogenomics—reconstruction of phylogenetic relationships based on mitochondrial genomic data—is now possible across large animal groups. Crustaceans in the class Malacostraca display a high diversity of body forms and include large number of ecologically and commercially important species. In this study, comprehensive and systematic analyses of the phylogenetic relationships within Malacostraca were conducted based on 86 mitochondrial genomes available from Gen Bank. Among 86 malacostracan mitochondrial genomes, 54 species have identical major gene arrangement(excluding t RNAs) to pancrustacean ground pattern,including six species from Stomatopoda, three species from Amphipoda, two krill, seven species from Dendrobranchiata(Decapoda), and 36 species from Pleocyemata(Decapoda). However, the other 32 mitochondrial genomes reported exhibit major gene rearrangements. Phylogenies based on Bayesian analyses of nucleotide sequences of the protein-coding genes produced a robust tree with 100% posterior probability at almost all nodes. The results indicate that Amphipoda and Isopoda cluster together(Edriophthalma)(BPP=100).Phylomitogenomic analyses strong support that Euphausiacea is nested within Decapoda, and closely related to Dendrobranchiata, which is also consistent with the evidence from developmental biology. Yet the taxonomic sampling of mitochondrial genome from Malacostraca is very biased to the order Decapoda, with no complete mitochondrial genomes reported from 11 of the 16 orders. Future researches on sequencing the mitochondrial genomes from a wide variety of malacostracans are necessary to further elucidate the phylogeny of this important group of animals. With the increase in mitochondrial genomes available, phylomitogenomics will emerge as an important component in the Tree of Life researches.     

北极Ny-Ålesund地区真菌多样性分析及龙胆苦苷转化菌株的筛选

采用多种培养基对北极高纬地区（土壤及海洋沉积物）真菌进行分离纯化,共得真菌65株。通过分析菌株的18S rDNA序列研究了它们的系统发育多样性,18S rDNA序列分析表明,65株真菌分属25个属,其中数量上位居第一的是青霉属（Penicillium）,有菌株20株,其次为丛赤壳属（Nectria）和节枝孢属（Articulospora）,各有菌株6株。同时对这65株真菌开展转化龙胆苦苷阳性菌株的筛选。以β-葡萄糖苷酶作为筛选转化菌株的标志酶于65株真菌中筛选到阳性菌株28株,其中产酶能力强的9株菌作为转化实验的备选菌株,以龙胆苦苷为底物进行转化,将转化粗提物进行TLC及HPLC分析,最终确定将C-5作为转化龙胆苦苷的菌株。根据菌株形态特征和核酸序列分析结果,初步鉴定菌株C-5为青霉属真菌。暂定名为Penicillium sp.C-5。     

山东省黄河下游地区部分县市高碘型甲状腺肿与地质环境的关系

地方病分布与地质环境中地形地貌、地质构造、地层岩性、土壤及水文地质条件有着密切的关系。本文根据山东省黄河下游区域地方病调查与生态地球化学调查的基础数据,从地形地貌、土壤、饮用水方面研究探讨了高碘型地甲病与地质环境的关系。研究表明调查区高碘型地甲病与饮水中碘含量具有非常好的正相关关系,土壤碘含量与高碘型地甲病不具有相关关系。在此基础上,提出了结合环境地质研究推进高碘型地甲病防治的意见和建议。     

内蒙古甲生盘铅锌硫矿床铅同位素组成及其对成矿物质来源的示踪意义

甲生盘铅锌硫矿床位于华北地台北缘狼山—渣尔泰山成矿带内,赋矿地层为中元古代渣尔泰山群的一套黑色岩系。铅同位素测试结果表明,甲生盘矿床铅同位素组成分为两组:A组以低放射性成因铅为特征,其同位素组成206Pb/204Pb为15.889~16.257,207Pb/204Pb为15.158~15.455,208Pb/204Pb为35.112~36.271;B组以相对高放射性成因铅为特征,其同位素组成206Pb/204Pb为17.871~18.990,207Pb/204Pb为15.509~15.672,208Pb/204Pb为37.525~38.770。矿石硫化物明显分为早期沉积的层纹状、浸染状细粒硫化物和后期脉状产出的粗粒硫化物,均含有A、B两组铅特征,前者以A组特征为主,后者以B组特征为主。根据铅构造模式图解及μ值等铅同位素参数综合分析:A组铅来自上地壳中的造山带,并且有幔源铅的补偿,成矿物质来自于古老的基底地层;B组铅主要来源于上地壳,部分受矿体北翼花岗岩侵入体的影响,成矿物质主要来自赋矿围岩;矿床铅具有多来源混合铅结构特征。     

米曲霉两步活化法对黄芩苷的生物转化及转化产物的纯化与鉴定

利用米曲霉把黄芩苷转化为黄芩素。转化条件为：液体发酵，pH6，30℃，利用综合马铃薯培养基与玉米芯培养基两步发酵。菌种采用两步活化法，将经综合马铃薯培养基培养48h的米曲霉加入至玉米芯培养基培养48h后，再加入底物黄芩苷继续培养3d。玉米芯发酵液加甲醇混匀后过滤，通过高效液相色谱检测，结果表明米曲霉已经将黄芩苷转化为黄芩素，转化率为40．72％。同时用乙醚萃取法从玉米芯发酵液中萃取到黄色固体提取物，经过高效液相色谱与标准品对照鉴定，该提取物为黄芩素。     

贡嘎山东坡亚高山森林系统植被光合作用——双裂蟹甲草（Cacalia davidii）净光合速率对生态因子的响应

以双裂蟹甲草(Cacaliadavidii)为材料，研究其净光合速率对生态因子的响应。结果表明，双裂蟹甲草净光合速率(P