Use of species-specific PCR for the identification of 10 sea cucumber species

We developed a species-specific PCR method to identify species among dehydrated products of 10 sea cucumber species. Ten reverse species-specific primers designed from the 16S rRNA gene, in combination with one forward universal primer, generated PCR fragments of ca. 270 bp length for each species. The specificity of the PCR assay was tested with DNA of samples of 21 sea cucumber species. Amplification was observed in specific species only. The species-specific PCR method we developed was successfully applied to authenticate species of commercial products of dehydrated sea cucumber, and was proven to be a useful, rapid, and low-cost technique to identify the origin of the sea cucumber product.     

Regional-scale identification of three-dimensional pattern of vegetation landscapes

The altitudinal pattern of vegetation is usually identified by field surveys,however,these can only provide discrete data on a local mountain.Few studies identifying and analyzing the altitudinal vegetation pattern on a regional scale are available.This study selected central Inner Mongolia as the study area,presented a method for extracting vegetation patterns in altitudinal and horizontal directions.The data included a vegetation map at a 1∶1 000 000 scale and a digital elevation model at a 1∶250 000 scale.The three-dimensional vegetation pattern indicated the distribution probability for each vegetation type and the transition zones between different vegetation landscapes.From low to high elevations,there were five vegetation types in the southern mountain flanks,including the montane steppe,broad-leaved forest,coniferous mixed forest,montane dwarf-scrub and sub-alpine shrub-meadow.Correspondingly,only four vegetation types were found in the northern flanks,except for the montane steppe.This study could provide a general model for understanding the complexity and diversity of mountain environment and landscape.     

A Test Pattern Identification Algorithm and Its Application to CINRAD/SA(B) Data简

A variety of faulty radar echoes may cause serious problems with radar data applications,especially radar data assimilation and quantitative precipitation estimates.In this study,＂test pattern＂ caused by test signal or radar hardware failures in CINRAD （China New Generation Weather Radar） SA and SB radar operational observations are investigated.In order to distinguish the test pattern from other types of radar echoes,such as precipitation,clear air and other non-meteorological echoes,five feature parameters including the effective reflectivity data percentage （Rz）,velocity RF （range folding） data percentage （RRF）,missing velocity data percentage （RM）,averaged along-azimuth reflectivity fluctuation （RNr,z） and averaged along-beam reflectivity fluctuation （RNa,z） are proposed.Based on the fuzzy logic method,a test pattern identification algorithm is developed,and the statistical results from all the different kinds of radar echoes indicate the performance of the algorithm.Analysis of two typical cases with heavy precipitation echoes located inside the test pattern are performed.The statistical results show that the test pattern identification algorithm performs well,since the test pattern is recognized in most cases.Besides,the algorithm can effectively remove the test pattern signal and retain strong precipitation echoes in heavy rainfall events.     

新一代天气雷达三维组网产品在人工防雹的应用

基于冰雹云的雷达三维拼图反射率分布形态特征,提出在人工影响防雹作业中识别冰雹云的6个候选指标,分别是组合反射率、云回波顶高、最大反射率回波顶高、垂直累积液态水含量、垂直累积液态水含量密度以及垂直累积液态水含量跃增值。基于新一代天气雷达组网数据应用风暴跟踪(SCIT)算法实现风暴跟踪并计算部分指标,形成模糊逻辑判断方法,确定防雹作业区域。结果表明,应用该方法对北京、江西的多个降雹个例进行三维风暴单体的冰雹云识别试验,对比地面降雹观测数据,计算多个个例模拟命中率77%,误报率26%,空报率22%。考虑组合反射率三维形态分布特征的模糊逻辑法可以较好地识别大部分可作业冰雹云,跟踪模拟效果较好,有利于在人工影响防雹作业中的有效应用。     

利用高精度遥感信息进行地面风险识别及评估

正一、引言高难山地地震勘探,地表条件复杂,地势险峻,悬崖峭壁众多,风险识别采用地形图和现场踏勘相结合的办法。由于我国大面积1∶50 000地形图绘制于20世纪50—80年代,地面信息有限,特别是经过近20年的快速发展,当年绘制的地面障碍和地形与目前实地有较大差异。现场踏勘,受时间限制不可能覆盖全部工区,且耗时耗力,真正完成工区地面风险源的识别并作出正确评估,往往要测量工序完成后才能实现,严重影响项目安全作业。如果在施工前就能够准确识别工区地表     

解译全景世界,工程化遥感解译又出新利器

正随着我国国产遥感卫星创新发展,综合运用空天地一体化的数据采集和处理手段也不断创新,新一代的工程化遥感解译系统——解译工厂(analyze factory)日前推出。2014年5月29日,在北京召开的"全景天地2014用户大会暨第五届eCognition(易康)中国用户大会"上,北京全景天地科技有限公司进行了解译工厂的发布。该一体化软硬件集群系统可实现各种遥感"大数据"的快速解译分析,从而实现了从影像到信息的工程化、智能化生产。据介绍,该系统采用了"多源影像解译知识库"专利与"工程化影像解译技术"2项核心技     

面向地图综合的矢量曲线弯曲识别研究

对矢量曲线的弯曲识别进行研究,从数学的角度解析了几种弯曲识别方法的原理,在Visual Studio 2008和ArcEngine 9.3基础上编程实现各算法并通过实验对比分析各种识别算法的优缺点。在制图综合中,可根据不同需要及各算法的利弊择优选取不同的识别方法对矢量曲线进行弯曲识别后再化简,既有利于线要素整体形状的自然保持,也可使化简综合的结果更为合理。     

应用属性数学理论的采空区地基稳定性评价

基于属性教学理论,本文提出了评价采空区地基稳定性的属性识别模型.以采煤工作面终采时间、沉降趋势、深厚比、构造复杂程度、上覆岩层强度、相对位置、“活化”因素等作为评价指标,将来空区稳定性等级划分为4个级别,以属性数学理论建立各评价指标的属性测度函数,以该函数计算单指标属性测度值和样本综合属性测度值,最后以置信度准则进行评...     

利用三值模板模糊匹配进行手指静脉识别

针对静脉特征图像中静脉边缘和末梢模糊造成匹配困难的问题,提出了一种基于三值模板图像的模糊匹配方法。该方法将提取出的静脉特征图像分割为三值图像,值1代表目标区,0.5代表模糊区,0代表背景区。三值图像用于模板匹配时,计算模板间非0值区域之间的平均距离作为相似分数,分数越低,则模板越相似。该方法匹配能力强,不要求点与点之间的绝对位置匹配,对模糊的静脉边缘和末梢匹配具有较强的鲁棒性。对实验中采集的456张近红外手指静脉图像进行匹配,正确识别率达99.46%,表明本文方法是可行的和有效的。     

计算几何法在风暴识别中的应用

风暴的合并与分裂对其发展有着重要的影响,是风暴识别与追踪工作中的难点问题。为了避免由资料处理过程引起的虚假合并,以及通过识别风暴的合并与分裂临界区域,对判断风暴的发展趋势提供有效的追踪参数,基于SCIT(Storm Cell Identification and Tracking Algorithm)风暴识别的基础,利用计算几何的凸壳与DT(Delaunay Triangle)算法,结合风暴内部的结构信息,识别出了风暴带以及风暴带合并与分裂的临界区域。并且给出了实现的个例以及算法的评价结果,为风暴合并与分裂的追踪工作提供了一个新的参数。