不同营养条件下铜绿微囊藻(Microcystis aeruginosa)和小球藻(Chlorella vulgaris)的生长竞争行为

蓝藻的暴发通常是藻类之间竞争的结果,了解环境中蓝藻与其它藻类的竞争特点和生存策略对揭示藻华暴发的机制及治理具有重要意义。本文以营养竞争为例,以铜绿微囊藻(Microcystis aeruginosa)和小球藻(Chlorella vulgaris)为受试对象,通过测定细胞密度、胞内C、N、P、S四种主要元素含量以及培养基中总氮(TN)、总磷(TP)的消耗,研究不同营养等级(超富营养、富营养、中营养、贫营养)以及单独N、P限制下两种藻在单独培养和共生培养条件下的生长特征和竞争行为。结果表明:(1)在超富营养、富营养、中营养、贫营养条件时,单独培养下的两种藻生物量与营养丰富程度呈正相关;(2)共培养条件下,在高营养水平时铜绿微囊藻在竞争中占优势,低营养水平时小球藻具有竞争优势;(3)胞内C、N、P、S的测定发现,随着营养水平的下降,两种藻胞内N、P的百分含量逐渐减少,而C、S并未呈现显著变化;(4)N限制时,铜绿微囊藻的半饱和常数Ks及最大比增长率μmax均大于小球藻;P限制条件下铜绿微囊藻的半饱和常数Ks小于小球藻,而最大比增长率μmax大于小球藻。综合分析,同一营养条件下,铜绿微囊藻竞争优势的先决因子是N,小球藻是P。因此,从营养竞争与生物适应力角度考虑,降低水体中富余的N或适当提高P的浓度可让小球藻获得竞争优势,对限制单一物种的形成具有平衡作用,可作为防治藻华的潜在方法。     

基于稳定同位素的东太平洋大青鲨(Prionace glauca)营养级研究

碳、氮稳定同位素为分析淡水和海洋生态系统的生物营养关系提供了有力手段,但在大洋鱼类中的应用还很少。本研究以热带东太平洋大青鲨(Prionace glauca)整节脊椎骨作为样品(叉长范围153—242cm),运用稳定同位素技术,分析大青鲨的δ~(13)C值和δ~(15)N值及其变化,比较不同基线生物的选择对营养级计算的影响。结果表明,大青鲨δ~(13)C范围为–15.76‰—–13.41‰,最大差值2.35‰;δ~(15)N范围为10.62‰—17.72‰,最大差值7.1‰;δ~(13)C值和δ~(15)N值随个体长度的变化不明显(可能原因是样品鱼均为较大个体),性别间的差异不显著,但不同基线生物的选择对营养级计算值的影响较大。本文研究表明,通过整节脊椎骨的稳定同位素测定来获得大青鲨的摄食特征信息是可行的。由于基线生物对营养级计算的影响问题难以在短期内解决,今后可将相对营养级或营养级的变化作为重点,研究人类活动(如捕捞)对大洋鱼类摄食和营养级的影响。     

福建龙海杨梅产地元素地球化学特征

福建省是中国杨梅重要产地,其中龙海市浮宫杨梅因其独特品质而名扬海外,是当地的名特优农产品。以龙海杨梅产地为研究区,系统采集了35套岩石-土壤-杨梅果实样品,测定了主量组分、植物营养有益元素、有害元素以及土壤部分营养元素有效态含量。研究表明：受地质背景影响,浮宫镇及其周边杨梅产地土壤中植物营养有益元素的有效度及其有效量普遍高于东泗镇,而有害元素含量较低,为浮宫杨梅的优质高产、富硒杨梅产出提供了地质地球化学条件;土壤中植物营养元素P、Mn、Cu、Zn、S有效态含量与其全量显著正相关;研究区岩石、土壤Se含量均远高于全国平均水平,采集的35件杨梅果实样品富硒率高达74.3%;植物营养有益元素的富集系数明显高于有害元素,显示出杨梅对营养有益元素的选择性吸收以及对有害元素的阻遏作用。     

不同营养方式下藻蓝蛋白对莱茵衣藻光合作用与生长的影响研究

藻蓝蛋白是光合蓝藻重要捕光天线藻胆体的主要组成部分,它能够帮助蓝藻吸收绿色光合生物难以利用的波长范围内的光,为了探究重组藻蓝蛋白对绿色植物光合作用的影响,本研究比较了在光合自养和混合营养两种营养方式下重组表达藻蓝蛋白的莱茵衣藻的光合作用与生长情况。结果表明：表达了藻蓝蛋白的莱茵衣藻中存在着从藻蓝蛋白到光合反应中心的光能传递。光合自养条件下,藻蓝蛋白能够显著促进莱茵衣藻的光合作用与生长;混合营养生长时,藻蓝蛋白也能够促进莱茵衣藻的光合作用,但效果不如光合自养条件下显著。     

荣成湾水产种质资源保护区生态环境质量综合评价

2015—2018年每年8月,对荣成湾国家级水产种质资源保护区表层海水中重金属、营养盐等环境因子连续监测调查,运用单因子指数法、综合质量指数法、有机污染指数法、富营养化指数法从不同方面分析了保护区的水质情况。结果表明,荣成湾保护区主要污染物质为Cd、Pb,水质总体符合二类海水水质标准;综合质量指数范围为0.202～0.615,评价等级为尚清洁状态;有机污染指数和富营养化指数变化范围分别为-0.853～1.133,0.002 28～0.376,保护区未受到有机污染并处于贫营养状态;保护区DIN含量低于山东省近岸海域的平均值,57%的调查站位存在DIP限制,营养盐含量匮乏。     

Seasonal variability in free-living marine nematode community structure in a sandy beach of the Taiping Bay of Qingdao, China

Nematode assemblage composition, trophic structure and biodiversity were followed over an annual cycle in a sandy beach of the Taiping Bay of Qingdao, China. Nematode assemblage in the sandy beach maintained a high genus diversity （75 genera）. Mlero- laimus and Bathylaimus were the dominant genus of the nematode assemblage, accounting for 66% of the total nematode abundance. The nematodes＇ dominant trophic structure changed seasonally as a response to the seasonal changes in food quality. Epigrowth-feeder nematodes （2A） were the dominant trophic groups in the trophic structure with the highest abundance in spring because of phytoplankton bloom, while the feeding type （ 1 B） showed higher abundance in summer that was due to the increasing of sediment detritus after spring bloom. Furthermore, species diversity and evenness calculated on nematodes identified to the genus level displayed significant temporal changes, which was also reflected by the index of trophic diversity. According to the cluster analysis, the nematode community structure of the whole year was clearly separated into two periods （A and B）. Biota-Envlron- ment matching （BIOENV） results showed that seawater temperature, sediment Chl a and grain size were responsible for the nema- tode community structure variation in spring and summer period （Period A）. However, seawater/interstitial water temperature, interstitial water dissolved oxygen concentration,interstitial water salinity, and sediment Ph a a were more important in constructing the autumn and winter period （Period B） nematode community structure.     

Nutrient composition and distributions in coastal waters impacted by the Changjiang plume

Four cruises were conducted during 2002--2003 in the Changjiang Estuary and adjacent coastal areas. The data presented show a clear coast to open sea gradient in nutrients related to the river inputs. Maximum values of chlorophyll a were typically observed at intermediate salinities at surface water and coincided with non-conservative decreases in nutrients along the salinity gradient, indicating that removal of nutrients was related to phytoplankton uptake. The seasonal variations of nutrient concentrations were just opposite to those of chlorophyll a, indicating that the seasonal variations of nutrients were mainly controlled by phytoplankton uptake, whereas riverine inputs merely weakened or balanced its extent. During the estuarine mixing, phosphate demonstrated some remobilization during all the four cruises; whereas both conservative and non-conservative behaviors for dissolved inorganic nitrogen and silicate were observed in the study area, indicating that both biotic and abiotic events may affect their behaviors during the estuarine mixing. Under the influence of freshwater inputs with high value of ratio of nitrogen to phosphorus, the estuarine and coastal waters impacted by the Changjiang plume were high （ 〉 30） in ratio of nitrogen to phosphorus, but rates of primary production were apparently not constrained by any kind of nutrient elements. However, the low （ 〈 1 ） ratio of silicate to nitrogen in most of the study area might be linked with the rapidly increasing frequency of harmful algal bloom （HAB） incidents in recent years in the coastal waters impacted by the Changjiang plume.     

氨基酸螯合微量元素的研究及其在水产动物中的应用

微量元素在水产动物的生命过程中是必不可少的,但是仅靠从水体中吸收是不能满足机体的正常需要的,还必须从饲料中摄取。至今,微量元素添加剂的发展经历了三代:第一代为无机盐类,常用的为硫酸盐等。无机盐类的微量元素添加剂易潮易结块,易与维生素发生拮抗,在植酸等抗营养因子存     

湛江海区8种常见海藻营养成分分析

对采自湛江沿海的8种常见海藻的营养成分进行分析。结果显示:碳水化合物是构成这8种藻体的主要成分,占藻体干重的48.22%～69.16%。蛋白质占2.81%～15.44%,平均为9.25%,且蛋白质中氨基酸含量高,平均16.01%,氨基酸组成中天冬氨酸、谷氨酸、甘氨酸、缬氨酸和亮氨酸含量多;绿藻门种类无论是蛋白质还是氨基酸含量都高于红藻门和褐藻门的种类,4种绿藻的氨基酸评分最高。粗脂肪占0.15%～1.17%,平均0.56%。粗纤维的平均含量近似于碳水化合物的一半。根据蛋白质和粗脂肪的比例,叉枝藻Cymogongrus flabelliformis和盾叶蕨藻Caulerpa racemosa var.peltata均可作为高蛋白、低脂肪的良好食物来源。矿物质中铁、锌含量丰富,介于0.311～1.722mg/g间,铜、砷和镍含量超标。     

厦门海域小型浮游动物和桡足类的摄食对浮游植物生长的影响

2005年11月16日和27日,运用稀释法和桡足类添加法,对厦门宝珠屿海域小型浮游动物及桡足类的摄食对浮游植物生长的影响进行了研究.结果表明,各粒级浮游植物的生长率均大于小型浮游动物的摄食率,小型浮游动物对总的Chla和nano-Chla具有一致的显著的摄食作用(0.51～0.78d-1),当存在螺旋环沟藻等大型的异养甲藻时,亦能摄食micro-级浮游植物.所添加的桡足类主要摄食micro-级的浮游植物,也显著摄食小型浮游动物,16日,所添加的桡足类促进nano-级浮游植物的每天生长效应达0.03ind/dm3.说明了厦门海域小型浮游动物及桡足类的摄食共同控制着浮游植物的生长,由于桡足类的杂食性,可产生一定的营养级联效应.