红鳍东方鲀(Takifugu rubrips)病原杀对虾弧菌的主要生物学特性研究

采用表观分类学及分子生物学方法,对从发病的养殖红鳍东方()中分离的细菌进行了主要生物学特性研究.主要包括形态特征、理化特性等较系统的表观分类学指征鉴定;同时对代表菌株进行了16S rRNA基因的分子鉴定,测定了16S rRNA基因序列、分析了相关细菌相应序列的同源性、构建了系统发生树;结果表明,分离菌为弧茵属(Vibrio Pacini 1954)的杀对虾弧菌(Vibrio penaeicidasp.nov.Ishimaru et al 1995).代表菌株(Hc051105-6株)16s rRNA基因序列长度(不包括引物结合区)为1443bp(GenBank登录号:EU073023),代表菌株(HC051105-8株)16S rRNA基因序列长度(不包括 引物结合区)为1450bp(GenBank登录号:Eu073024).择代表菌株做对健康红鳍东方鲀的人工感染试验,认为分离鉴定的杀对虾弧菌在被检红鳍东方鱼电病例具有一定的病原学意义.药敏试验结果显示,对供试37种抗菌药物中,在一些药物中存在耐药性的菌株间差异.     

异育银鲫气单胞菌病原菌鉴定和药敏试验

从患病异育银鲫体内分离到5株可疑菌株,经API 20NE细菌生化鉴定,YCS07-03和YZ07-11株鉴定率为99.2%和99.3%。注射感染中5×108 CFU.mL-1剂量组表现出较强的致病性,12 d死亡率100%。菌株在营养肉汤培养液中呈均匀混浊状;在营养琼脂平皿中呈灰白色半透明菌落;在血琼脂培养基中呈光滑灰白色菌落,YZ07-11株出现透明的β溶血环;在TCBS培养基呈黄色菌落;在SS和麦康凯培养基中形成无色透明的扁平菌落。经负染电镜观察,菌株两端圆,呈直杆状,无芽孢,极生单鞭毛,菌株大小(短径×长径)分别为1.53μm×2.71μm和0.80μm×2.12μm。菌株经生化实验鉴定为温和气单胞菌(Aeromonas sobria)和嗜水气单胞菌(Aeromonas hydrophila)。试验结果表明:菌株对丁胺卡那霉素、头孢噻肟、庆大霉素、左氟沙星、复合磺胺、氟嗪酸、洛美沙星、复方新诺明、链霉素和新霉素均产生高度敏感;对万古霉素为中度敏感;氨苄青霉素、羧苄青霉素、强力霉素和青霉素产生耐药。     

高层建筑电梯平衡量体系的动力特性与地震反应分析

本文在文献〔２，１〕所建立的平衡重体系运动方程的基础上，对高层建筑电梯系统中平衡重体系的动力特性进行了探讨，给出了求解该体系自振频率的近似方法，通过对一幢１４层建筑内的平衡重体系进行的大量地震反应分析，研究了其动力反应沿建筑物高度变化的一般规律，给出了最大反应所处的位置。本文的工作为实施和推广文献〔１〕所提出的平衡重体系的抗震控制措施奠定了理论基础。     

固结过程对冻土应力-应变特性的影响

选用四种不同固结方式,并在－５０℃条件下,对冻结砂土样进行三轴压缩试验,试验发现固结过程对冻土的强度与变表有明显影响。经历Ｋ０ＤＣＦ的冻土其强度最大,友坏变形最小,经历ＦＣ的试样强度最小,破坏变形最大;经历ＥＤＣＦ和ＥＵＤＣＦ的产介于中间。     

天然感潮河道水流紊动特性分析

采用多谱勒三相流速仪，在长江口徐六泾水文观测断面分别进行了涨潮、落潮时中泓与近岸垂线的流速观测，根据这些观测资料，对天然感潮河段潮流紊动的周期、频率、概率密度函数等进行了定量的数学描述，并对时均流速、紊动强度、雷诺应力等沿垂线分布进行了分析计算。     

水分胁迫对东方百合光合特性、叶绿素荧光参数及干物质积累的影响

 以东方百合“索邦”为试材,在科尔沁沙地对其在不同水分胁迫下的光合特性、叶绿素荧光及干物质积累进行了研究。结果显示,随着水分胁迫的加剧,净光合速率(Pn)和蒸腾速率(Tr)明显下降。另外,水分胁迫下百合叶片发生了“光合午休”现象,气孔导度(gs)也呈下降的趋势,胞间二氧化碳浓度(Ci)先下降后升高,并且通过gs和Ci变化方向可以判断出,百合叶片净光合速率受气孔与非气孔两种因素的限制。百合叶片最小荧光(Fo)无明显变化,最大荧光(Fm)、可变荧光(Fv)和光系统Ⅱ(PSⅡ)最大光化学效率(Fv/Fm)下降较明显。百合总干重及各器官干重也随水分胁迫的加剧而明显下降。研究表明,水分胁迫降低了百合叶片净光合速率,抑制了PSⅡ的光化学活性,从而影响到了百合干物质的积累。因此,在科尔沁沙地,应该采取合理的灌溉技术来保证百合在生长过程中得到充足的水分,以此确保切花百合的品质。     

Assessing seawater quality with a variable fuzzy recognition model

With the rapid development of the marine economy industry, human exploitation of marine resources is increasing, which is contributing to the growing trend of eutrophication and frequent occurrence of red tide. Accordingly, investigations of seawater quality have attracted a great deal of attention. This study was conducted to construct a seawater environmental quality assessment model based on the variable fuzzy recognition model. The uncertainty and ambiguity of the seawater quality assessment were then considered, combining the monitoring values of evaluation indicators with the standard values of seawater quality. Laizhou Bay was subsequently selected for a case study. In this study, the correct variable model for different parameters was obtained according to the linear and nonlinear features of evaluation objects. Application of the variable fuzzy recognition model for Laizhou Bay, water quality evaluation and comparison with performance obtained using other approaches revealed that the generated model is more reliable than traditional methods, can more reasonably determine the water quality of various samples, and is more suitable for evaluation of a multi-index, multi-level, nonlinear marine environment system; accordingly, the generated model will be an effective tool for seawater quality evaluation.     

STUDY ON THE CHARACTERISTICS OF TROPICAL CYCLONES ACTIVITY OVER THE WESTERN NORTH PACIFIC IN 2004 AND THE CAUSATION

In this paper, we summarized the characteristics of tropical cyclones (TC) activity over the western North Pacific in 2004 and analyzed their causation. Compared with the normal, the annual frequency of TC in 2004 was slightly higher, tropical cyclones in 2004 had a longer life span and occurred in a concentrated period, the source of TC were situated eastward; in all tracks of TC, the recurvature tracks took up larger proportion, the landfall regions of TC were located northward, which concentrated from East China to Japan. The primary causes were revealed as follows. Firstly, the intensity and area of the western North Pacific subtropical high was stronger and larger than usual respectively, and its ridge was frequently in the form of cells and stretched northwestward. Secondly, the convergence of intertropical convergence zone (ITCZ) was reinforced and the convergence zone moved more eastward than average. Thirdly, the meridionality of the westerlies was larger than average and the cell-shaped ridge formed a saddle region, which is in favor of TC northward motion and recurature.     

Stokes波的谱特性分析

应用理论推导及数值计算方法，对Stokes随机波的谱特性进行了分析。首先将波面方程，海水质点水平速度用一阶波面分量的非线性组合表示，应用平稳随机高阶短的降阶计算法则，得到了波面方程及海水质点水平速度与一阶波面分量的自相关函数之间的关系，从而确定了Stokes随机波浪的波浪谱密度及海水质点水平速度和加速度谱密度，进而求得有关波浪要素的均方根值。文章还应有数值计算方法，分析了波浪基本参数对均方根值的影响。